Effects of Genic Base Composition on Growth Rate in G+C
... Pseudomonas aeruginosa. In C. cresentus, the strains expressing G+C-rich gene variants had higher growth rates than strains producing the identical protein from G+C-poor gene variants, mirroring results previously reported for E. coli (Raghavan et al. 2012). In both C. cresentus and E. coli, selecti ...
... Pseudomonas aeruginosa. In C. cresentus, the strains expressing G+C-rich gene variants had higher growth rates than strains producing the identical protein from G+C-poor gene variants, mirroring results previously reported for E. coli (Raghavan et al. 2012). In both C. cresentus and E. coli, selecti ...
ISEcp1 pneumoniae Research Article
... blaTEM and blaSHV revealed that TEM gene was present in 19(20%), SHV in 8(8.4%) and both TEM and SHV genes in 67.3 percent (64) of the isolates studied [10]. Our study on molecular characterization of CTX-M, TEM and SHV ESBLs revealed a higher proportion of CTX-M (49.5%) than TEM (24.8%) and SHV (7. ...
... blaTEM and blaSHV revealed that TEM gene was present in 19(20%), SHV in 8(8.4%) and both TEM and SHV genes in 67.3 percent (64) of the isolates studied [10]. Our study on molecular characterization of CTX-M, TEM and SHV ESBLs revealed a higher proportion of CTX-M (49.5%) than TEM (24.8%) and SHV (7. ...
Genome Research 17
... (P = 0.002), with the ratio roughly 30% larger for coding sequences that mapped to the Z chromosome. The avian karyotype differs from that of many other organisms by showing significant heterogeneity in chromosome size, including a large number of very small (<20 Mb) chromosomes, the “microchromosom ...
... (P = 0.002), with the ratio roughly 30% larger for coding sequences that mapped to the Z chromosome. The avian karyotype differs from that of many other organisms by showing significant heterogeneity in chromosome size, including a large number of very small (<20 Mb) chromosomes, the “microchromosom ...
DNA - Wise Science
... 2. RNA polymerase uses one strand of DNA as a template to produce a complimentary strand of RNA (Remember A-U in RNA). 3. RNA detaches from the DNA and transcription is complete. Transcription produces three types of RNA molecules: 1. Messenger RNA (mRNA) – takes the DNA message for conversion to pr ...
... 2. RNA polymerase uses one strand of DNA as a template to produce a complimentary strand of RNA (Remember A-U in RNA). 3. RNA detaches from the DNA and transcription is complete. Transcription produces three types of RNA molecules: 1. Messenger RNA (mRNA) – takes the DNA message for conversion to pr ...
November 2010 Prof Angela van Daal Forensic DNA
... Flanking regions are the stretches of DNA outside the region of interest. For STRs for example, these sequences are the non-repeated DNA regions which, unlike the repeat regions, are are the same amongst individuals. The primer sequences are designed from DNA in the flanking regions such that they w ...
... Flanking regions are the stretches of DNA outside the region of interest. For STRs for example, these sequences are the non-repeated DNA regions which, unlike the repeat regions, are are the same amongst individuals. The primer sequences are designed from DNA in the flanking regions such that they w ...
Poster Abstracts - Ecological Genomics Institute
... rufiabdominalis and S. avenae as the second and third most common aphids, respectively. The aphid species that were found to overlap between wheat and big bluestem were R. padi and S. avenae, with these being the first and second most common aphids in big bluestem, respectively. Results from summer ...
... rufiabdominalis and S. avenae as the second and third most common aphids, respectively. The aphid species that were found to overlap between wheat and big bluestem were R. padi and S. avenae, with these being the first and second most common aphids in big bluestem, respectively. Results from summer ...
Mycobacterium kyorinense sp. nov., a novel, slow
... The sequence of the 16S rRNA gene was identical in all strains, but was different from all other available 16S rRNA sequences. The sequences of the hsp65, rpoB and sodA genes were also identical in the three isolates. It was assumed that these three isolates belong to the same species, and strain KU ...
... The sequence of the 16S rRNA gene was identical in all strains, but was different from all other available 16S rRNA sequences. The sequences of the hsp65, rpoB and sodA genes were also identical in the three isolates. It was assumed that these three isolates belong to the same species, and strain KU ...
Genotyping BoLA-DRB3 alleles in Brazilian Dairy Gir cattle (Bos
... alleles. This methodology cannot accurately determine differences between all current alleles, and this may have led to the different conclusions in disease association studies. Another technique, which has been used for typing HLA genes, is polymerase chain reaction using sequencespecific oligonucl ...
... alleles. This methodology cannot accurately determine differences between all current alleles, and this may have led to the different conclusions in disease association studies. Another technique, which has been used for typing HLA genes, is polymerase chain reaction using sequencespecific oligonucl ...
microbial growth requirements
... Criteria a culture media must meet. must contain the appropriate nutrients. must contain sufficient moisture. must contain a properly adjusted pH. must contain suitable levels of oxygen. media must initially be sterile. Culture media may be a liquid (broth) or a solid (agar) . The agar is the solidi ...
... Criteria a culture media must meet. must contain the appropriate nutrients. must contain sufficient moisture. must contain a properly adjusted pH. must contain suitable levels of oxygen. media must initially be sterile. Culture media may be a liquid (broth) or a solid (agar) . The agar is the solidi ...
Infinium Multi-Ethnic EUR/EAS/SAS BeadChip
... a. Estimated sample throughput based on use of 1 HiScan System, 1 AutoLoader 2.x, 1 Tecan robot, and a 5-day work week. b. Values are derived from genotyping 708 HapMap reference samples. c. Value expected for typical projects using standard Illumina protocols. Tumor samples and samples prepared ...
... a. Estimated sample throughput based on use of 1 HiScan System, 1 AutoLoader 2.x, 1 Tecan robot, and a 5-day work week. b. Values are derived from genotyping 708 HapMap reference samples. c. Value expected for typical projects using standard Illumina protocols. Tumor samples and samples prepared ...
ppt - Chair of Computational Biology
... analysis of B.subtilis genomic data. The total height of the four letters in each position indicates the position specific information content, while the height of each letter is proportional to the nucleotide frequency. ...
... analysis of B.subtilis genomic data. The total height of the four letters in each position indicates the position specific information content, while the height of each letter is proportional to the nucleotide frequency. ...
Intergeneric transfer of Panton-Valentine Leukocidin
... Bacteriophage are currently researched for medical purposes, so that they may be used to lyse and kill harmful bacteria without affecting other human bodily functions. This is an issue since bacteriophage pick up and transfer new DNA from the host bacteria they lyse and transfer it to new bacteria. ...
... Bacteriophage are currently researched for medical purposes, so that they may be used to lyse and kill harmful bacteria without affecting other human bodily functions. This is an issue since bacteriophage pick up and transfer new DNA from the host bacteria they lyse and transfer it to new bacteria. ...
Testing enhancers predicted by high constraint
... transcription factor expressed in early development and mutated in Townes-Brocks syndrome19 (Fig. 2). Seven of the elements flanking SALL1 directed tissue-specific reporter gene expression in the transgenic in vivo assay, recapitulating aspects of SALL1’s endogenous expression characteristics at e11 ...
... transcription factor expressed in early development and mutated in Townes-Brocks syndrome19 (Fig. 2). Seven of the elements flanking SALL1 directed tissue-specific reporter gene expression in the transgenic in vivo assay, recapitulating aspects of SALL1’s endogenous expression characteristics at e11 ...
Genetic Linkage Mapping of Zebrafish Genes and
... panel (Dr.261, Dr.274, Dr.345, Dr.1339). These may represent genes with pseudogenes or they may be pairs of duplicate genes generated by chromosomal duplications. ...
... panel (Dr.261, Dr.274, Dr.345, Dr.1339). These may represent genes with pseudogenes or they may be pairs of duplicate genes generated by chromosomal duplications. ...
Globin gene family
... from each other between 2 and 4 billion years ago • Highly conserved genes can be studied in one model organism, and the results applied to other organisms Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
... from each other between 2 and 4 billion years ago • Highly conserved genes can be studied in one model organism, and the results applied to other organisms Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
Analysis and Evolution of Two Functional Y
... Based on sequence divergence for the internal transcribed spacer (ITS) sequences of ribosomal DNA (Desfeux et al. 1996), using accepted rates of silent site substitutions in plants (Gaut 1998), we estimate that in the genus Silene, the sex chromosomes probably evolved about 20 MYA. Plant sex chromos ...
... Based on sequence divergence for the internal transcribed spacer (ITS) sequences of ribosomal DNA (Desfeux et al. 1996), using accepted rates of silent site substitutions in plants (Gaut 1998), we estimate that in the genus Silene, the sex chromosomes probably evolved about 20 MYA. Plant sex chromos ...
Rec.DNA.BCH 446,31-32
... – Vector DNA functions to insert and amplify the DNA of intersit . • Vectors should contain an origin of replication – Enables the vector, together with the foreign DNA fragment inserted into it, to replicate • they contain one or more single (unique) restriction endonuclease sites that provide a ch ...
... – Vector DNA functions to insert and amplify the DNA of intersit . • Vectors should contain an origin of replication – Enables the vector, together with the foreign DNA fragment inserted into it, to replicate • they contain one or more single (unique) restriction endonuclease sites that provide a ch ...
Designing Microarray Experiments
... need to worry about how to best pair the samples. The most common design is the reference design, which puts the same control sample on every array. Dye swap is not necessary and statistical analysis is simple, but … you need twice as many arrays as in designs that use both channels. There are lots ...
... need to worry about how to best pair the samples. The most common design is the reference design, which puts the same control sample on every array. Dye swap is not necessary and statistical analysis is simple, but … you need twice as many arrays as in designs that use both channels. There are lots ...
Pathway analysis
... • Pathway analysis should probably be called information enrichment analysis – a more accurate term • Used prudently, it is a useful tool for exploring the functional landscape of an experiment • To make it meaningful, you need to interpret the results in the context of the specific biology under st ...
... • Pathway analysis should probably be called information enrichment analysis – a more accurate term • Used prudently, it is a useful tool for exploring the functional landscape of an experiment • To make it meaningful, you need to interpret the results in the context of the specific biology under st ...
PowerPoint Presentation - Aminosugar metabolism
... antifolate drugs are commonly used in cancer chemotherapy and as antimicrobials. Plants, fungi, and bacteria synthesize the precursor DHF de novo from GTP and p-aminobenzoic acid (pABA). The NADPH-dependent reduction of DHF catalyzed by DHFR is essential both in the de novo and in the recycling path ...
... antifolate drugs are commonly used in cancer chemotherapy and as antimicrobials. Plants, fungi, and bacteria synthesize the precursor DHF de novo from GTP and p-aminobenzoic acid (pABA). The NADPH-dependent reduction of DHF catalyzed by DHFR is essential both in the de novo and in the recycling path ...
Bacterial Transformation - Tamalpais Union High School
... bacteria to another Color Marker gene- Betagalactosidase-produces enzyme that breaks down lactose Antibiotic Resistance: Some bacteria have genes coding for enzymes that destroy certain antibiotics! ...
... bacteria to another Color Marker gene- Betagalactosidase-produces enzyme that breaks down lactose Antibiotic Resistance: Some bacteria have genes coding for enzymes that destroy certain antibiotics! ...
Where is the root of the universal tree of life?
... proteins used to root the tree were plagued with unrecognised paralogies and mostly with unequal rates of evolution. More recently, we have shown that most positions are saturated with respect to amino acid substitutions in the six data sets used to root the universal tree till now.(12) This makes t ...
... proteins used to root the tree were plagued with unrecognised paralogies and mostly with unequal rates of evolution. More recently, we have shown that most positions are saturated with respect to amino acid substitutions in the six data sets used to root the universal tree till now.(12) This makes t ...
Rick Kittles, PhD Uses Golden Helix Software to Identify Novel
... with those smaller studies, money,’ I respond that it’s there’s still over a million SNPs. better to have one program It’s been very easy to manage that can do everything than those large datasets using to have to look for updates SVS’s data sheet management on multiple applications all system.” Mos ...
... with those smaller studies, money,’ I respond that it’s there’s still over a million SNPs. better to have one program It’s been very easy to manage that can do everything than those large datasets using to have to look for updates SVS’s data sheet management on multiple applications all system.” Mos ...
2. Methods and Data Analysis - National Genetics Reference
... changes, termed as ‘silent’, are only used to back up information gained from new peaks. The analysis was carried out by SEQUENOM using SNP Discovery software. The version of the program used was specifically designed to analyse target sequences in a (large) sample sets for SNP discovery. Consequent ...
... changes, termed as ‘silent’, are only used to back up information gained from new peaks. The analysis was carried out by SEQUENOM using SNP Discovery software. The version of the program used was specifically designed to analyse target sequences in a (large) sample sets for SNP discovery. Consequent ...
a database designed for the polymorphisms of the human ccr2 gene
... We have developed a specialized database that contains information about polymorphic markers (predominantly SNPs) in the CCR2 gene and its neighboring DNA regions, their population frequencies and also about the trait and diseases associated with these polymorphisms. The database consists of 4 inter ...
... We have developed a specialized database that contains information about polymorphic markers (predominantly SNPs) in the CCR2 gene and its neighboring DNA regions, their population frequencies and also about the trait and diseases associated with these polymorphisms. The database consists of 4 inter ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.