Genome-wide analysis of DNA copy-number
... approximately 85% specificity (15% false positives) for detection of single-copy gene ‘deletion’ (using a decision threshold at the point where the distributions of fluorescence ratios for autosomal and X-chromosomal genes crossed, at ∼1 s.d. apart from their means). By using a ‘moving average’ of f ...
... approximately 85% specificity (15% false positives) for detection of single-copy gene ‘deletion’ (using a decision threshold at the point where the distributions of fluorescence ratios for autosomal and X-chromosomal genes crossed, at ∼1 s.d. apart from their means). By using a ‘moving average’ of f ...
Comparison of Target-Capture and Restriction
... ultraconserved elements (UCEs), are emerging as two of the most popular methods for phylogenomics using reducedrepresentation genomic data sets. These two methods were designed to target different evolutionary timescales: RAD-seq was designed for population-genomic level questions and UCEs for deepe ...
... ultraconserved elements (UCEs), are emerging as two of the most popular methods for phylogenomics using reducedrepresentation genomic data sets. These two methods were designed to target different evolutionary timescales: RAD-seq was designed for population-genomic level questions and UCEs for deepe ...
The S RNA segment of tomato spotted wilt virus has an ambisense
... endogenous translation products in the absence of R N A transcripts; lane 2, translation products of TMV R N A ( M r 126K and 183K); lane 3, translation products of CCMV R N A ( M r 23K, 35K, 100K and 105K); lanes 4 and 7, translation products directed by the pTSWV-vcORF transcripts; lane 6, transla ...
... endogenous translation products in the absence of R N A transcripts; lane 2, translation products of TMV R N A ( M r 126K and 183K); lane 3, translation products of CCMV R N A ( M r 23K, 35K, 100K and 105K); lanes 4 and 7, translation products directed by the pTSWV-vcORF transcripts; lane 6, transla ...
FEMS symposium on microbial communities in soil
... methods such as direct and indirect inmunofluorescence, tube agglutinacion, íammodif fusión, ii3Bimo«lectrophoresis , capillary precipitación Cecbttiques and radioaccive techniques are üapractical for large scale field scudies. In recent years, Azospirillma bras líense has b«en used worldwide as a c ...
... methods such as direct and indirect inmunofluorescence, tube agglutinacion, íammodif fusión, ii3Bimo«lectrophoresis , capillary precipitación Cecbttiques and radioaccive techniques are üapractical for large scale field scudies. In recent years, Azospirillma bras líense has b«en used worldwide as a c ...
Restriction enzymes
... usually shows two lines for each of the STRs tested. This is because usually, the STRs inherited from the parents are of different lengths. Occasionally, only one line appears because both STRs in a pair are of the same length. • When the DNA profile of a child is compared to the profiles of the chi ...
... usually shows two lines for each of the STRs tested. This is because usually, the STRs inherited from the parents are of different lengths. Occasionally, only one line appears because both STRs in a pair are of the same length. • When the DNA profile of a child is compared to the profiles of the chi ...
DNA Analysis
... Sir Alec Jereys is credited with developing DNA profiling using RFLP. In September of 1984, after years of work, he saw his first series of blots on an X ray. The technique was first used in forensics when, in 1985, he was asked by police to confirm the rape confession of 17-year-old Richard Buckla ...
... Sir Alec Jereys is credited with developing DNA profiling using RFLP. In September of 1984, after years of work, he saw his first series of blots on an X ray. The technique was first used in forensics when, in 1985, he was asked by police to confirm the rape confession of 17-year-old Richard Buckla ...
Forensics Ch 12
... cooling, and strand rebuilding is repeated typically 25 to 30 times, yielding more than one million copies of the original DNA molecule. Each cycle takes less than two minutes from start to finish. ...
... cooling, and strand rebuilding is repeated typically 25 to 30 times, yielding more than one million copies of the original DNA molecule. Each cycle takes less than two minutes from start to finish. ...
Alpha Thalassemia - ARUP Lab Test Directory
... o An extra functional α-globin gene present Deletion or duplication of unknown clinical significance detected • Breakpoints of large deletions/duplications will not be determined; therefore, it may not be possible to distinguish mutations of similar size • This assay does not assess for nondeletiona ...
... o An extra functional α-globin gene present Deletion or duplication of unknown clinical significance detected • Breakpoints of large deletions/duplications will not be determined; therefore, it may not be possible to distinguish mutations of similar size • This assay does not assess for nondeletiona ...
Holoprosencephaly Panel, Nonsyndromic Sequencing and Deletion
... Holoprosencephaly Panel, Nonsyndromic, Sequencing and Deletion/Duplication, 11 Genes 2008848 • Preferred test for individuals with clinical phenotype of HPE and a normal karyotype Holoprosencephaly Sequencing, 11 Genes 2008853 • Acceptable test for individuals with clinical phenotype of HPE and a no ...
... Holoprosencephaly Panel, Nonsyndromic, Sequencing and Deletion/Duplication, 11 Genes 2008848 • Preferred test for individuals with clinical phenotype of HPE and a normal karyotype Holoprosencephaly Sequencing, 11 Genes 2008853 • Acceptable test for individuals with clinical phenotype of HPE and a no ...
FEBS Letters
... pVK100 [19]. These cosmids were in vitro packaged into lambda particles (Gigapack III Gold Packaging Extract, Stratagene, Amsterdam, The Netherlands) and were used to infect E. coli TG1 [18]. 1384 TcR KmS separate clones were obtained harboring mainly 12^30 kbp segments of the S. leopoliensis genome ...
... pVK100 [19]. These cosmids were in vitro packaged into lambda particles (Gigapack III Gold Packaging Extract, Stratagene, Amsterdam, The Netherlands) and were used to infect E. coli TG1 [18]. 1384 TcR KmS separate clones were obtained harboring mainly 12^30 kbp segments of the S. leopoliensis genome ...
Comprehensive and Rapid Genotyping of Mutations - HAL
... stored at -20°C in 96-well plates so that both PCRs and sequence reactions set-up can be done with pipetting robot or multichannel pipettors. PCRs were performed in 96-well plates, in a 25-µl final volume containing 1X PCR Master Mix (Promega, Madison, WI), 3.2 pmol of each PCR primer and 10 ng of ...
... stored at -20°C in 96-well plates so that both PCRs and sequence reactions set-up can be done with pipetting robot or multichannel pipettors. PCRs were performed in 96-well plates, in a 25-µl final volume containing 1X PCR Master Mix (Promega, Madison, WI), 3.2 pmol of each PCR primer and 10 ng of ...
Where Is DNA Found?
... strand rebuilding is repeated typically 25 to 30 times, yielding more than one million copies of the original DNA molecule. Each cycle takes less than two minutes from start to finish. ...
... strand rebuilding is repeated typically 25 to 30 times, yielding more than one million copies of the original DNA molecule. Each cycle takes less than two minutes from start to finish. ...
Sequences 5` to Translation Start Regulate
... 1985a). Although the rbcS genes show a high degree of homology within the coding region and also in the 5'- and 3'-flanking regions (Dean et al., 1985b; Dean et al., 1987a), there is a 100-fold range in expression levels in leaf tissue (Dean et al., 1985b; Dean et al., 1987b). We are investigating t ...
... 1985a). Although the rbcS genes show a high degree of homology within the coding region and also in the 5'- and 3'-flanking regions (Dean et al., 1985b; Dean et al., 1987a), there is a 100-fold range in expression levels in leaf tissue (Dean et al., 1985b; Dean et al., 1987b). We are investigating t ...
A pseudogene cluster in the leader region of the Euglena
... base pairing 1n stem regions. These alternations rendered 1t unable to function normally, and thus this gene was identified as a pseudogene. Similar consideration might also be possible for the DNA segment corresponding to the tRNA Ala gene region, although, in this case, the tRNA A1a anticodon 1s a ...
... base pairing 1n stem regions. These alternations rendered 1t unable to function normally, and thus this gene was identified as a pseudogene. Similar consideration might also be possible for the DNA segment corresponding to the tRNA Ala gene region, although, in this case, the tRNA A1a anticodon 1s a ...
Microarray Data Analysis
... one sample from the training set and uses it as a test sample. The remaining training samples are used to predict the removed test sample. Best predictor genes obtained in training phase will also selected from the test samples for further analysis. Example of Cross-validation in AML ALL data set (3 ...
... one sample from the training set and uses it as a test sample. The remaining training samples are used to predict the removed test sample. Best predictor genes obtained in training phase will also selected from the test samples for further analysis. Example of Cross-validation in AML ALL data set (3 ...
KOD -Plus
... blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201], KOD -Plus- Neo [Code No. KOD-401] or KOD FX [Code No. KFX-101]. The kit contains pTA2 Vector, 2x Ligation Buffer, T4 DNA Ligase and 10x A-attachment Mix. 10 x A-attachment mix is a reagent comprising anti-KOD DNA polymerase antibo ...
... blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201], KOD -Plus- Neo [Code No. KOD-401] or KOD FX [Code No. KFX-101]. The kit contains pTA2 Vector, 2x Ligation Buffer, T4 DNA Ligase and 10x A-attachment Mix. 10 x A-attachment mix is a reagent comprising anti-KOD DNA polymerase antibo ...
Genetics, environment and cognitive abilities
... With just 350 markers (i.e. at 10 cM intervals ± roughly one in every 10 million base pairs throughout the 3.5 billion in the genome), it is possible to search the genome for linkage because each marker can scan millions of base pairs for a gene of large effect. However, linkage cannot detect genes ...
... With just 350 markers (i.e. at 10 cM intervals ± roughly one in every 10 million base pairs throughout the 3.5 billion in the genome), it is possible to search the genome for linkage because each marker can scan millions of base pairs for a gene of large effect. However, linkage cannot detect genes ...
Kingdom Prokaryotae (Monera)
... selective pressure) Their origin is ancient (began when earth is quite different: reduced atmosphere, acid pools, hot volcanic habitats) ...
... selective pressure) Their origin is ancient (began when earth is quite different: reduced atmosphere, acid pools, hot volcanic habitats) ...
What`s in a Genotype? - CEUR Workshop Proceedings
... Historically and today, biologists have explored the basis of biology and disease by correlating genotype with phenotype, wherein a genotype represents the genetic composition of a phenotype - a physical trait as realized in a certain environment. This paradigm has supported research in human and mo ...
... Historically and today, biologists have explored the basis of biology and disease by correlating genotype with phenotype, wherein a genotype represents the genetic composition of a phenotype - a physical trait as realized in a certain environment. This paradigm has supported research in human and mo ...
Variable and Feature Selection in Machine Learning (Review
... => the classifier’s performance usually will degrade for a large number of features! In many cases the information that is lost by discarding variables is made up for by a more accurate mapping/sampling in the lower-dimensional space ! ...
... => the classifier’s performance usually will degrade for a large number of features! In many cases the information that is lost by discarding variables is made up for by a more accurate mapping/sampling in the lower-dimensional space ! ...
Alpha Thalassemia - Lab Test Directory
... o An extra functional α-globin gene present Deletion or duplication of unknown clinical significance detected • Breakpoints of large deletions/duplications will not be determined; therefore, it may not be possible to distinguish mutations of similar size • This assay does not assess for nondeletiona ...
... o An extra functional α-globin gene present Deletion or duplication of unknown clinical significance detected • Breakpoints of large deletions/duplications will not be determined; therefore, it may not be possible to distinguish mutations of similar size • This assay does not assess for nondeletiona ...
pdf
... genera Azoarcus and Thauera were further characterized in terms of their taxonomic and metabolic diversity related to aromatic compound degradation [17]. Denitrifying bacterial consortia capable of degrading 4-chlorobenzoate were characterized with terminal restriction fragment length polymorphism ( ...
... genera Azoarcus and Thauera were further characterized in terms of their taxonomic and metabolic diversity related to aromatic compound degradation [17]. Denitrifying bacterial consortia capable of degrading 4-chlorobenzoate were characterized with terminal restriction fragment length polymorphism ( ...
Determining the Relationship between Phlyctimantis and Kassina
... It is possible that Kassina is the sister taxa to Phlyctimantis - an idea that has been tossed around for a while but has had no confirmation. It has also been suggested that they are all members of the same genus. To try to get some resolution about the position of Phlyctimantis within the “kassino ...
... It is possible that Kassina is the sister taxa to Phlyctimantis - an idea that has been tossed around for a while but has had no confirmation. It has also been suggested that they are all members of the same genus. To try to get some resolution about the position of Phlyctimantis within the “kassino ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.