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Learning Target #1: Know vocabulary that builds the
Learning Target #1: Know vocabulary that builds the

... ______ 3. The process by which a cell makes a copy of the DNA. ______ 4. The building blocks of a protein. ______ 5. One form of a gene. ______ 6. An organism’s genetic makeup or the letters used to represent the trait. ______ 7. A chart or “family tree” that tracks the inheritance of a particular t ...
Genome Assembly and Annotation
Genome Assembly and Annotation

... • Public genome initiative used clone-by-clone strategy, private used WGS • Merits heavily debated, combination might be preferable ...
PPT - Larry Smarr - California Institute for Telecommunications and
PPT - Larry Smarr - California Institute for Telecommunications and

... • For a variation to be considered a SNP, it must occur in at least 1% of the population • SNPs make up about 90% of all human genetic variation • SNPs occur every 100 to 300 bases along the 3-billion-base human genome • Many SNPs have no effect on cell function, but scientists believe others could ...
Biology UNIT 2 Heredity: Inheritance and Variation of traits Big Ideas
Biology UNIT 2 Heredity: Inheritance and Variation of traits Big Ideas

...  All cells contain genetic information in the form of DNA molecules. Genes are regions in the DNA that contain the instructions that code for the formation of proteins. (secondary to HS-LS3-1) (Note: This Disciplinary Core Idea is also addressed by HS-LS1-1.) LS3.A: Inheritance of Traits  Each chr ...
Genetic Diseases and Gene Therapy
Genetic Diseases and Gene Therapy

... • What are the differences between cloning, recombinant DNA, and genetic engineering? • What are the tools we use for genetic engineering? – Plasmids – Restriction Enzymes – DNA Ligase ...
Agriculture - eduBuzz.org
Agriculture - eduBuzz.org

...  The bacteria Bacillus thuringiensis produces proteins (known as BT toxins) that have been used for insecticides  The toxin is isolated from the bacteria genome and inserted into Ti plasmid of agrobacterium  The resulting plant kills insects that eat it ...
Recitation Section 17 Answer Key Recombinant DNA and Cloning
Recitation Section 17 Answer Key Recombinant DNA and Cloning

... Cloning DNA means to isolate a gene or fragment of DNA away from the other DNA of an organism and be able to propagate this piece. 2. What is a vector? Vectors (or plasmids) are naturally occurring small pieces of circular DNA that can replicate in various organisms. In this course we concentrate on ...
Bi 430 / 530 Theory of Recombinant DNA Techniques Syllabus
Bi 430 / 530 Theory of Recombinant DNA Techniques Syllabus

... modified in the test tube? Why is PCR such a versatile tool for nucleic acid studies? What DNA sequences exist in nature, and what are they for? How are computers used to manage and study biological information? How can all of the genes in a genome be studied at once? How is DNA moved into and betwe ...
Emanuel BS, Warren ST , Garber KB. The human genome: a diamond in the rough. Curr Opin Genet Dev. 2012 Jun;22(3):189-90. doi: 10.1016/j.gde.2012.04.005. Epub 2012 May 18. No abstract available.
Emanuel BS, Warren ST , Garber KB. The human genome: a diamond in the rough. Curr Opin Genet Dev. 2012 Jun;22(3):189-90. doi: 10.1016/j.gde.2012.04.005. Epub 2012 May 18. No abstract available.

... complete picture of the human genome. One could argue, though, that we still only have a rough understanding of how to interpret a full genome sequence and that we need to move from an understanding of individual genes towards an understanding of genomes. A major advance that the Human Genome Projec ...
2421_Ch9.ppt
2421_Ch9.ppt

... by creating a desired sequence of nucleotides DRAWBACKS to synthetic DNA  can only make short sequences (120 nucleotides) – usually too short for an entire gene  must link all the short sequences together to form the whole gene  must already know the desired sequence to be synthesized (can be pre ...
Bacterial Comparative Genomics
Bacterial Comparative Genomics

... • If two genes are orthologs, that does not imply they have same function, but they often do • If two genes are paralogs, they have traditionally thought to often differ in function, and paralogy is thought to be one of the main sources of “new” genes • …but there is some evidence to suggest that pa ...
Genes for Speed or Endurance?
Genes for Speed or Endurance?

... Slow twitch fibres are more efficient in using oxygen to generate energy, while fast twitch fibres are less efficient in energy generation. Genetics The DNA molecule is the carrier of genetic information. Genes consist of the four types of DNA building bases called A, C, G, & T. The order of these b ...
day2
day2

first of four for Chapter 9
first of four for Chapter 9

... • These plasmids with multiple resistance genes are called R plasmids, and cause ...
Communication_files/Bad Presentation #3
Communication_files/Bad Presentation #3

... Escherichia coli ...
Recombinant DNA Biotech Summary Questions
Recombinant DNA Biotech Summary Questions

Vocabulary:
Vocabulary:

... forms  words  and  sentences  that  help  you  understand  the  author’s  meaning.   The  alphabet  of  your  DNA  only  has  4  letters:  A,C,G,  and  T!  These  letters  aren’t   the  same  as  the  letters  of  our  alphabet;  th ...
Genetic changes - Southington Public Schools
Genetic changes - Southington Public Schools

... Mutations in gametes will be passed to the offspring. Types of mutations 1. Point mutations—a change in a single base of a DNA chain. This results in a different “message.” Example: normal sequence  THE DOG BIT THE CAT mutation  THE DOG BIT THE CAR Sense mutation: the changed codon makes a differe ...
Ligation and Transformation
Ligation and Transformation

... Overall Transformation Process 1. The plasmid vector must be cut with a restriction endonuclease (aka: restriction enzyme) 2. DNA ligase joins the DNA fragment & vector DNA 3. Host cell is made competent so can plasmid can enter 4. Transformed cells are grown on selection media ...
DNA Mutations - U
DNA Mutations - U

... Cells have the ability to repair damages, but as an organism ages, DNA repair does not work as effectively; thus changes occur in the DNA ...
OGM - unisalento.it
OGM - unisalento.it

... pest-resistant genes inserted into crop plants might escape into other plants, creating hard-tocontrol superweeds. There is also the possibility of unexpected effects on other flora and fauna, the risk of agriculture being controlled by biotech companies, and, as with any new technology, problems th ...
Cloning Class - Open Bio Labs
Cloning Class - Open Bio Labs

... genes as its parent. Molecular cloning: a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. Transformation: the genetic alteration of a cell resulting from the direct uptake and incorporation of ...
How can recombinant DNA be used?
How can recombinant DNA be used?

... •Frostban strawberries- protects strawberries from bacteria that cause frost to form •Pesticide resistant plants- pesticides will kills pests (weeds, insects, etc.) but not plants •Oncomous – mouse with a gene for cancer (for research) •Pharming- inserting genes into livestock so they produce pharma ...
From the principle of heredity to the molecular - diss.fu
From the principle of heredity to the molecular - diss.fu

... been characterised by rapid advances during the last 65 years. With the sequencing of several genomes, including that of man, contemporary molecular genetics is now focussing on genotype – phenotype correlations as a means of identifying functions for each of the human genes. ...
Citrus Breeding - Aggie Horticulture
Citrus Breeding - Aggie Horticulture

... • Natural mechanism for species to maintain genetic uniformity- pummelo, mandarins • Serious inbreeding depression in citrus overcome by apomixis- nucellar embryony • Important for gene inheritance and function studies ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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