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pptx format
pptx format

... Humans combined from billions of cells. ...
Whole Exome Sequencing
Whole Exome Sequencing

... Exons tell our body how to make proteins. Proteins tell the cell what kind of cell it will be, such as a muscle cell, a brain cell, a skin cell etc., and how that cell should act. All the exons of our genes combined together are called the exome. Most of the changes in genes that cause medical probl ...
Exam 2
Exam 2

... ____31. When a typical restriction enzyme cuts a DNA molecule, the cuts are staggered so that the DNA fragments have singlestranded ends. This is important in recombinant DNA work because _____. A. it allows a cell to recognize fragments produced by the enzyme B. the single-stranded ends serve as st ...
Hutchinson Gilford Progeria Syndrome: A
Hutchinson Gilford Progeria Syndrome: A

... a trans-activating CRISPR RNA (tracrRNA), and the Cas9 protein. Portions of the DNA of the invading agent, usually a virus, are inserted within the bacterial genomic CRISPR loci. Those sequences are transcribed into specific crRNA. crRNAs determine the target DNA sequence that will be manipulated by ...
Document
Document

... Interchange. Representation of alphanumeric and some special characters as 1-byte (8 bit) unsigned integers {0 ... 255} (the set {20-1 ... 28-1}). The ASCII character set also includes nonprinting control characters such as carriage return (CR) or line feed (LF). Minimum storage requirement for huma ...
Week 5 EOC Review DNA, Mitosis, Meiosis, and Genetics
Week 5 EOC Review DNA, Mitosis, Meiosis, and Genetics

... Which of the following carries amino acids to the site of protein synthesis? A. mRNA B. rRNA C. tRNA D. nRNA ...
Determination of Transgenic Loci by Expression FISH
Determination of Transgenic Loci by Expression FISH

... in the trans configuration. Because of the high intensity of signal at the transcription site, it was possible to remove spurious fluorescence or diffuse signal by establishing a threshold level for the images. Signal was verified by the single bright foci for each color present in most cells. The m ...
File
File

File
File

... choosing only females, dairy farmers can be guaranteed that all of their calves will be female, rather than having to use their resources to raise ...
Genetic Recombination in Eukaryotes
Genetic Recombination in Eukaryotes

... greater chance of recombination by crossingover • 1% recombinants = 1 map unit (m.u.) • 1 m.u. = 1 centiMorgan (cM) ...
Genterapi – The future of medicine
Genterapi – The future of medicine

... feature to construct as viruses have this as an fundamental function of their lifecycle. What is more difficult is to make the virus unable to reproduce on its own. A good analogy to what might happen if this isn’t insured is this. Imagine taking a drug in the form of a pill. Once the pill is swallo ...
Photosynthesis - Mrs. Brenner's Biology
Photosynthesis - Mrs. Brenner's Biology

... Cleaves vector (plasmid) and foreign (human) DNA. Cleaving DNA makes DNA fragments ending in short single-stranded segments with “sticky ends.” The “sticky ends” allow insertion of foreign DNA into vector DNA. Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or displa ...
Exam 3
Exam 3

... another. This shift alters the hydrogen bonding between bases which results in improper basepairing, allowing the tautomerized base to pair with bases other than the one it is normally paired with during DNA replication. Base analogues are compounds sufficiently similar to basepair with the correct ...
Introduction Presentation
Introduction Presentation

... 4. How the parents’ genetic code (genotype), and their genetics-based biochemical, physical and behavioral traits (an organism’s phenotype) are inherited by their progeny 5. How naturally-occurring “markers” in this genetic code (DNA sequence) can inform questions re. fish population structure and e ...
Genetic engineering
Genetic engineering

... This method is called Southern blotting, after its inventor, Edwin Southern. ...
Ch 13 student notes
Ch 13 student notes

Bz gene identification
Bz gene identification

... open it online http://www.dnai.org/media/bioinformatics/genefinding/bzgeneprediction_ws.doc. If you opened this document in an Internet browser click File, click Save as, and save it to a directory on your C- or A-drives. Then, close the browser, open the document in MS Word, and follow the instruct ...
DNA Profiles
DNA Profiles

... Microarray Testing  Each field of the microarray contains a unique sequence of single-stranded DNA  Test DNA and normal DNA are converted to single strands, tagged with fluorescent dyes, and hybridized to the chip  Each result has a different color • Normal alone is green; mutant alone is red • ...
Question In the last 100 years… What is Feed Efficiency?
Question In the last 100 years… What is Feed Efficiency?

Bio 101 Homework 2 Prof. Fournier
Bio 101 Homework 2 Prof. Fournier

... 68. For many years, humans have used a variety of techniques that have influenced the genetic makeup of organisms. These techniques have led to the production of new varieties of organisms that possess characteristics that are useful to humans. Identify one technique presently being used to alter th ...
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses

... Both use naturally occurring mutations and genetic recombinations. ...
Exam Review - Roosevelt High School
Exam Review - Roosevelt High School

File
File

... The technique of chromosome painting is the result of scientific research. Scientists use chromosome painting to mark the locations of genes on human chromosomes with fluorescent tags. Its also possible to apply this technique to the chromosomes of many different species. Chromosome painting allows ...
recombinant dna technology
recombinant dna technology

... THE SAME STICKY ENDS CARRIED BY THE FRAGMENTS • MIXING THE FRAGMENTS WITH THE CUT PLASMIDS ALLOWS BASE-PAIRING AT THE STICKY ENDS. • APPLICATION OF DNA LIGASE STABILIZES THE ATTACHMENT. • THE RECOMBINANT PLASMID IS THEN INTRODUCED INTO A BACTERIUM BY TRANSFORMATION ...
GOBASE—a database of organelle and bacterial
GOBASE—a database of organelle and bacterial

... all of which have been collected and verified by expert curators. Gene and product names are assigned from a standardized list maintained internally, to allow for ease of searching and sorting. This assembly of data is made available to researchers through an intuitive interface allowing for a wide ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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