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BeefTalk 644: It`s All About DNA As our scientific endeavors
BeefTalk 644: It`s All About DNA As our scientific endeavors

... technology is being utilized to read or see something we  could not in the past. How we use this new‐found information still is in the early stages of development.  However, if one thinks back to those wonderful building blocks we played with as a child, those same  principles would be in play.  ...
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... • multiple copies (is usually advantageous) • several unique RE localized to a specific region (mcs) • convenient method for detection of cloned DNA ...
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variation in fitness - University of California, Berkeley

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16.6 * Locating and Sequencing Genes

... Remember that each tube probably contains millions of copies of the DNA template, countless nucleotides, and a good supply of the specific terminator nucleotide. Due to this, you get a variety of ‘partially completed’ DNA strands, because they have been ‘terminated’ at different points. ...
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Mutation



In biology, a mutation is a permanent change of the nucleotide sequence of the genome of an organism, virus, or extrachromosomal DNA or other genetic elements. Mutations result from damage to DNA which is not repaired or to RNA genomes (typically caused by radiation or chemical mutagens), errors in the process of replication, or from the insertion or deletion of segments of DNA by mobile genetic elements. Mutations may or may not produce discernible changes in the observable characteristics (phenotype) of an organism. Mutations play a part in both normal and abnormal biological processes including: evolution, cancer, and the development of the immune system, including junctional diversity.Mutation can result in several different types of change in sequences. Mutations in genes can either have no effect, alter the product of a gene, or prevent the gene from functioning properly or completely. Mutations can also occur in nongenic regions. One study on genetic variations between different species of Drosophila suggests that, if a mutation changes a protein produced by a gene, the result is likely to be harmful, with an estimated 70 percent of amino acid polymorphisms that have damaging effects, and the remainder being either neutral or weakly beneficial. Due to the damaging effects that mutations can have on genes, organisms have mechanisms such as DNA repair to prevent or correct mutations by reverting the mutated sequence back to its original state.
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