Activation of the Interleukin-3 Gene by Chromosome
... the locations of the two cloned breakpoints in relation to the IL-3 gene. The two chromosome 5 breakpoints were separated by less than 500 bp. The genomic structure in Cases 1 and 2 suggested that a normal IL-3 gene product was over-expressed as a result of the altered promotor structure. This would ...
... the locations of the two cloned breakpoints in relation to the IL-3 gene. The two chromosome 5 breakpoints were separated by less than 500 bp. The genomic structure in Cases 1 and 2 suggested that a normal IL-3 gene product was over-expressed as a result of the altered promotor structure. This would ...
Expression and characterization of 1
... AL of sterile distilled water. The DNA concentration of the sample was determined spectrophotometrically at 260 nm. The digested DNA was then ligated to the pET-30a vector (Novagen) which had been previously digested with BamHI and HindIII and purified the same way as the bpoolQ of ACC deaminase gen ...
... AL of sterile distilled water. The DNA concentration of the sample was determined spectrophotometrically at 260 nm. The digested DNA was then ligated to the pET-30a vector (Novagen) which had been previously digested with BamHI and HindIII and purified the same way as the bpoolQ of ACC deaminase gen ...
Selecting Degenerate Multiplex PCR Primers
... input sequences. Both MC-DPD and MD-DPD have been shown to be NP-Hard problems [11]. In this paper, we describe the Multiple Degenerate Primer Design Problem (MDPD), present an algorithm to solve this problem and describe how the results can be used to select a large set of primers that can be used ...
... input sequences. Both MC-DPD and MD-DPD have been shown to be NP-Hard problems [11]. In this paper, we describe the Multiple Degenerate Primer Design Problem (MDPD), present an algorithm to solve this problem and describe how the results can be used to select a large set of primers that can be used ...
A High Density Integrated Genetic Linkage Map of Soybean and the
... and contained a total of 1015 SSR loci (Song et al., 2004). More recently, single DNA base differences between homologous DNA fragments, plus small insertions and deletions (INDELs), collectively referred to as SNPs, were shown to be the most abundant source of DNA polymorphisms in soybean (Hyten e ...
... and contained a total of 1015 SSR loci (Song et al., 2004). More recently, single DNA base differences between homologous DNA fragments, plus small insertions and deletions (INDELs), collectively referred to as SNPs, were shown to be the most abundant source of DNA polymorphisms in soybean (Hyten e ...
The Mitochondrial Genome of Chara vulgaris
... results of overall genome comparisons with MultiPipMaker (Schwartz et al., 2000). It can be seen that for many Chara genes, the greatest scores of sequence similarity are found with Marchantia and/or angiosperm mtDNAs. In addition, for some Chara genes (e.g., rpl5, rpl6, rpl16, and rps3), the region ...
... results of overall genome comparisons with MultiPipMaker (Schwartz et al., 2000). It can be seen that for many Chara genes, the greatest scores of sequence similarity are found with Marchantia and/or angiosperm mtDNAs. In addition, for some Chara genes (e.g., rpl5, rpl6, rpl16, and rps3), the region ...
Comparative gene mapping in Arabidopsis lyrata chromosomes 6
... the formation of the A. thaliana chromosomes. Like A. thaliana chromosome I, chromosome IV evolved from two A. lyrata chromosomes, but, rather than a fusion, there was a reciprocal translocation between AL6 and AL7, with the loss of an NOR probably present on A. lyrata chromosome 7 (Lysak et al., 20 ...
... the formation of the A. thaliana chromosomes. Like A. thaliana chromosome I, chromosome IV evolved from two A. lyrata chromosomes, but, rather than a fusion, there was a reciprocal translocation between AL6 and AL7, with the loss of an NOR probably present on A. lyrata chromosome 7 (Lysak et al., 20 ...
Identification of the mRNA targets of tRNA
... response to rare-tRNA induced ribosomal pausing. Translation is the most resource- and energy-consuming process in the cell, and is therefore highly regulated by a range of protein trans factors in response to environment and nutrient availability (16–22). However, while it is clear that tRNA concen ...
... response to rare-tRNA induced ribosomal pausing. Translation is the most resource- and energy-consuming process in the cell, and is therefore highly regulated by a range of protein trans factors in response to environment and nutrient availability (16–22). However, while it is clear that tRNA concen ...
... into close proximity [11-13]. 3C technology detects interactions between DNA regions by in vivo crosslinking of chromatin and PCR with primers specific for novel junctions created by ligation of sequences that were in close proximity through specific interactions. To determine if the Kcnq1 promoter ...
CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI The
... chromatid. Interestingly, we sometimes detected an unaltered product in addition to the rearranged product with the missegregated haplotype. We hypothesize that these two products may be generated by breakage of a partially replicated sequence near a replication fork with only one side of the break ...
... chromatid. Interestingly, we sometimes detected an unaltered product in addition to the rearranged product with the missegregated haplotype. We hypothesize that these two products may be generated by breakage of a partially replicated sequence near a replication fork with only one side of the break ...
Disintegrin, hemorrhagic, and proteolytic activities of Mohave
... lacked Mojave toxin. Two disintegrin genes, scutustatin and mojavestatin, were identified by PCR of genomic sequences. Scutustatin is a highly conserved disintegrin, while mojavestatin shows low conservation to other known disintegrins. Venoms with the highest LD50 measurements lacked both disintegr ...
... lacked Mojave toxin. Two disintegrin genes, scutustatin and mojavestatin, were identified by PCR of genomic sequences. Scutustatin is a highly conserved disintegrin, while mojavestatin shows low conservation to other known disintegrins. Venoms with the highest LD50 measurements lacked both disintegr ...
Transcriptional Attenuation
... Transcriptional attenuation might, in the broadest sense, include any phenomenon that reduces the extent or rate of transcription. In studies of bacterial gene regulation, however, it has acquired a more restricted definition and is used to describe a mechanism in which the level of transcriptional ...
... Transcriptional attenuation might, in the broadest sense, include any phenomenon that reduces the extent or rate of transcription. In studies of bacterial gene regulation, however, it has acquired a more restricted definition and is used to describe a mechanism in which the level of transcriptional ...
Fanconi anemia and RAD50 deficiency: genetic and functional
... Basic research involving human chromosomal instability and cancer susceptibility syndromes is necessary to gain insights into molecular mechanisms which maintain genomic integrity. The focus of this thesis is directed at the molecular, cellular and clinical aspects of two groups of inherited disease ...
... Basic research involving human chromosomal instability and cancer susceptibility syndromes is necessary to gain insights into molecular mechanisms which maintain genomic integrity. The focus of this thesis is directed at the molecular, cellular and clinical aspects of two groups of inherited disease ...
Identity-by-descent filtering of exome sequence data for disease
... past several decades has mainly proceeded by means of positional cloning to identify chromosomal linkage intervals followed by the sequencing of candidate genes (Collins, 1995). Efforts at diseasegene identification involving linkage analysis or association studies usually result in a genomic interv ...
... past several decades has mainly proceeded by means of positional cloning to identify chromosomal linkage intervals followed by the sequencing of candidate genes (Collins, 1995). Efforts at diseasegene identification involving linkage analysis or association studies usually result in a genomic interv ...
Replication timing and transcriptional control: beyond
... assays for replication [29]. There are only two mammalian loci for which both replication-timing switches and origin localization have been studied. At the mouse immunoglobulin IgH locus, new origins are activated in pre-B cells when the promoter region of this locus becomes transcriptionally active ...
... assays for replication [29]. There are only two mammalian loci for which both replication-timing switches and origin localization have been studied. At the mouse immunoglobulin IgH locus, new origins are activated in pre-B cells when the promoter region of this locus becomes transcriptionally active ...
Mitochondrial diseases and the role of the yeast models
... possible molecular mechanisms underlying the disease have been described here. The introduction of POLG mutations associated with progressive external ophthalmoplegia (PEO), L304R, 467T, G923D, R943H, Y955C, A957S, into yeast MIP1 caused increased mtDNA mutability, increased nuclear mutation rates a ...
... possible molecular mechanisms underlying the disease have been described here. The introduction of POLG mutations associated with progressive external ophthalmoplegia (PEO), L304R, 467T, G923D, R943H, Y955C, A957S, into yeast MIP1 caused increased mtDNA mutability, increased nuclear mutation rates a ...
Methods of Human Heredity Study
... attributes (for example, position of centromere). This term was coined by Russian scientist G.A. Levitzky in 1924. In all types of higher organisms (eukaryota), the well-organized nucleus contains definite number of chromosomes of definite size and shape. The term karyotype is given to the group of ...
... attributes (for example, position of centromere). This term was coined by Russian scientist G.A. Levitzky in 1924. In all types of higher organisms (eukaryota), the well-organized nucleus contains definite number of chromosomes of definite size and shape. The term karyotype is given to the group of ...
Investigating the DNA-Binding Interactions of Small
... thank the Goodson Group members for their help and support. I consider the Goodson group to be family. I would also like to thank my collaborators (Prof. Wilson, Prof. Imperiali, Prof. Laine) and dissertation committee members (Prof. Chen, Prof. Kuroda, Prof. Robertson). Finally, I would like to tha ...
... thank the Goodson Group members for their help and support. I consider the Goodson group to be family. I would also like to thank my collaborators (Prof. Wilson, Prof. Imperiali, Prof. Laine) and dissertation committee members (Prof. Chen, Prof. Kuroda, Prof. Robertson). Finally, I would like to tha ...
CFTR Mutations in Congenital Absence of Vas Deferens
... either the brothers with CBAVD inherited two different alleles from one of their parents (18) or in which fertile brothers inherited the same CFTR alleles as their brothers with CBAVD. Such discordance between phenotypes and marker haplotypes suggested that CBAVD was not caused by two mutated CFTR a ...
... either the brothers with CBAVD inherited two different alleles from one of their parents (18) or in which fertile brothers inherited the same CFTR alleles as their brothers with CBAVD. Such discordance between phenotypes and marker haplotypes suggested that CBAVD was not caused by two mutated CFTR a ...
2008 HSC Notes from the Marking Centre Biology
... principles and ideas. Many candidates wrote a lot of information that was not relevant to the question. Some responses showed evidence of rote learning of an anticipated answer based on a single source. These responses did not address the syllabus content and/or outcomes being assessed and hence did ...
... principles and ideas. Many candidates wrote a lot of information that was not relevant to the question. Some responses showed evidence of rote learning of an anticipated answer based on a single source. These responses did not address the syllabus content and/or outcomes being assessed and hence did ...
PDF
... Separate assays were developed to detect Mutation 1 (c.ins356T) and 2 (c.C.T406), and a third assay was designed which could detect both the adjacent Mutations 3 (c.del474T) and 4 (c.A.C475), as well as determine the phase of the 2 mutations. In all 3 assays, a fluorescent dye was incorporated into ...
... Separate assays were developed to detect Mutation 1 (c.ins356T) and 2 (c.C.T406), and a third assay was designed which could detect both the adjacent Mutations 3 (c.del474T) and 4 (c.A.C475), as well as determine the phase of the 2 mutations. In all 3 assays, a fluorescent dye was incorporated into ...
Development of a repressible mycobacterial
... both Gram positive and Gram negative bacteria, only a few of them have been successfully used in mycobacteria. The first regulated gene expression system to be widely exploited in mycobacteria is based on the acetamideinducible promoter of Mycobacterium smegmatis. It is very complex, based on two pos ...
... both Gram positive and Gram negative bacteria, only a few of them have been successfully used in mycobacteria. The first regulated gene expression system to be widely exploited in mycobacteria is based on the acetamideinducible promoter of Mycobacterium smegmatis. It is very complex, based on two pos ...
Recurrent Pregnancy Loss and Its Relation to Combined Parental
... Current results showed the maternal mutated thrombophilic genes’ profiles mainly have a potential risk for RPL but paternal mutation profiles in specific genes may also have a combined effect on RPL phenomenon. Study of mutations in a wide range of thrombophilic genes associated with the disease m ...
... Current results showed the maternal mutated thrombophilic genes’ profiles mainly have a potential risk for RPL but paternal mutation profiles in specific genes may also have a combined effect on RPL phenomenon. Study of mutations in a wide range of thrombophilic genes associated with the disease m ...
Rh phenotype prediction by DNA typing and its
... found with weak D antigen expression encode aberrant RhD with amino acid substitutions (Flegel et al., 1998). We noted that in all weak D samples characterized at the molecular level the substituted residues were located in transmembranous and intracellular RhD protein segments (Wagner et al., 1998b ...
... found with weak D antigen expression encode aberrant RhD with amino acid substitutions (Flegel et al., 1998). We noted that in all weak D samples characterized at the molecular level the substituted residues were located in transmembranous and intracellular RhD protein segments (Wagner et al., 1998b ...
Mechanisms of translational regulation in bacteria
... Consequently, the genetic code is degenerate: Except for tryptophan and methionine, the amino acids are encoded by two, four or six different so called synonymous codons. Hence an organism can tune the codon sequence according to its needs without changing the amino acid sequence of the expressed pr ...
... Consequently, the genetic code is degenerate: Except for tryptophan and methionine, the amino acids are encoded by two, four or six different so called synonymous codons. Hence an organism can tune the codon sequence according to its needs without changing the amino acid sequence of the expressed pr ...