Deletions of NF1 gene and exons detected by multiplex ligation
... Usually, NF1 diagnostic screening strategies employ PCRbased screening methods such as the protein truncation test (PTT),17 25 single-strand conformational polymorphism (SSCP),26 or denaturing high-performance liquid chromatography (DHPLC)15 27 with varying degrees of sensitivity for each method. Di ...
... Usually, NF1 diagnostic screening strategies employ PCRbased screening methods such as the protein truncation test (PTT),17 25 single-strand conformational polymorphism (SSCP),26 or denaturing high-performance liquid chromatography (DHPLC)15 27 with varying degrees of sensitivity for each method. Di ...
Cytogenetics with special reference to domestic animals
... Conserved synteny between the human and mouse genomes. Regions from different mouse chromosomes (indicated by the colors of each mouse in B) show conserved synteny (gene order) with the indicated regions of the human genome (A). For example the genes present in the upper portion of human chromosome ...
... Conserved synteny between the human and mouse genomes. Regions from different mouse chromosomes (indicated by the colors of each mouse in B) show conserved synteny (gene order) with the indicated regions of the human genome (A). For example the genes present in the upper portion of human chromosome ...
Effect of the Polymorphisms of Keratin Associated Protein 8.2 Gene
... cashmere fibre diameter; in long-term artificial fertilization and selection the excellent trait is always primarily preserved. The sequencing results showed that two unreported mutations, A214G and T218C were identified in the KAP8.2 gene. A214G did not change the amino acid, while T218C led to an ...
... cashmere fibre diameter; in long-term artificial fertilization and selection the excellent trait is always primarily preserved. The sequencing results showed that two unreported mutations, A214G and T218C were identified in the KAP8.2 gene. A214G did not change the amino acid, while T218C led to an ...
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
... • Larger fragments move more slowly through the agarose gel matrix than small fragments and hence the fragments are size fractionated • The resolution of separation depends on the type and concentration of gel and the length of the run: ‚mini‘ gels are fairly low resolution and typically used for se ...
... • Larger fragments move more slowly through the agarose gel matrix than small fragments and hence the fragments are size fractionated • The resolution of separation depends on the type and concentration of gel and the length of the run: ‚mini‘ gels are fairly low resolution and typically used for se ...
What is p53
... the term "p53" appears in the title! This massive interest in a single protein is almost unprecedented and reflects the central place of p53 in the regulation of cell number and the frequency with which abnormalities of p53 occur in human tumours. p53 has been named Molecule of the Year by the edito ...
... the term "p53" appears in the title! This massive interest in a single protein is almost unprecedented and reflects the central place of p53 in the regulation of cell number and the frequency with which abnormalities of p53 occur in human tumours. p53 has been named Molecule of the Year by the edito ...
Emended description of the order Chlamydiales, proposal of
... (Everett & Andersen, 1997). The 16s rDNA from strain SFPD was resequenced; the 16s rRNA and 16s-23s intergenic spacer sequence for strain WSU 86-1044 were provided by Fred Rurangirwa (Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA), The 23s rRNA se ...
... (Everett & Andersen, 1997). The 16s rDNA from strain SFPD was resequenced; the 16s rRNA and 16s-23s intergenic spacer sequence for strain WSU 86-1044 were provided by Fred Rurangirwa (Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA), The 23s rRNA se ...
SLOs - 3.3 Genetics small - Miss Jan`s Science Wikispace
... Explain how metabolic pathways are regulated in prokaryotes through gene induction Explain what a repressor is Explain how the end-product of a metabolic pathway can activate a repressor and switch genes off (gene repression) Explain the difference between an inducible and non-inducible syst ...
... Explain how metabolic pathways are regulated in prokaryotes through gene induction Explain what a repressor is Explain how the end-product of a metabolic pathway can activate a repressor and switch genes off (gene repression) Explain the difference between an inducible and non-inducible syst ...
Array-based sequencing of filaggrin gene for
... The filaggrin gene (FLG) is essential for skin differentiation and epidermal barrier formation with links to skin diseases, however it has a highly repetitive nucleotide sequence containing very limited stretches of unique nucleotides for precise mapping to reference genomes. Sequencing strategies u ...
... The filaggrin gene (FLG) is essential for skin differentiation and epidermal barrier formation with links to skin diseases, however it has a highly repetitive nucleotide sequence containing very limited stretches of unique nucleotides for precise mapping to reference genomes. Sequencing strategies u ...
Oxalate decarboxylase of the white-rot fungus
... Dichomitus squalens, and cloned the corresponding Ds-odc gene. The primary structure of the Ds-ODC protein contains two conserved Mn-binding cupin motifs, but at the N-terminus, a unique, approximately 60 aa alanine-serine-rich region is found. Real-time quantitative RT-PCR analysis confirmed gene e ...
... Dichomitus squalens, and cloned the corresponding Ds-odc gene. The primary structure of the Ds-ODC protein contains two conserved Mn-binding cupin motifs, but at the N-terminus, a unique, approximately 60 aa alanine-serine-rich region is found. Real-time quantitative RT-PCR analysis confirmed gene e ...
Designing synthetic MLPA probes - MRC
... accession number. A good overview of the various transcript variants of a gene can be obtained on the Entrez Gene website: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene. For exon numbering, which cannot be found in NM_sequences anymore, look for corresponding NG_sequence here: http://www.ncbi.nlm ...
... accession number. A good overview of the various transcript variants of a gene can be obtained on the Entrez Gene website: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene. For exon numbering, which cannot be found in NM_sequences anymore, look for corresponding NG_sequence here: http://www.ncbi.nlm ...
Why are most organelle genomes transmitted maternally?
... (sorting-out) of genetically distinct organelles (Box 1; Fig. 1), and the virtual absence of recombination [1, 2]. Due to the different evolutionary origins and inheritance modes of the genomes of the eukaryotic cell, severe evolutionary consequences arise: (i) Nuclear and organellar genomes differ ...
... (sorting-out) of genetically distinct organelles (Box 1; Fig. 1), and the virtual absence of recombination [1, 2]. Due to the different evolutionary origins and inheritance modes of the genomes of the eukaryotic cell, severe evolutionary consequences arise: (i) Nuclear and organellar genomes differ ...
Chromosomal translocations deregulated BCL6
... within BCL6 exons 4-5 which, based on the organization of the der(3) junctions, should be retained in both normal and abnormal BCL6 RNA species. For both SM1444 and KC1445 RNA, two RNA species were identified based on a nucleotide sequence analysis of the cloned RACE products. The first species show ...
... within BCL6 exons 4-5 which, based on the organization of the der(3) junctions, should be retained in both normal and abnormal BCL6 RNA species. For both SM1444 and KC1445 RNA, two RNA species were identified based on a nucleotide sequence analysis of the cloned RACE products. The first species show ...
Studies on the Mechanisms of Homolog Pairing and Sister
... Without their encouragement and understanding it would have been impossible for me to finish this work. ...
... Without their encouragement and understanding it would have been impossible for me to finish this work. ...
Epigenetics & Chromatin Xist through transcriptional control of Dnmt3a
... Interwoven into this complex regulatory circuitry there is an additional pathway that ensures only a single Xist gene is expressed in XX cells, and that the single Xist allele in XY cells remains repressed. The classical model for this process invokes the presence of a blocking factor present at lim ...
... Interwoven into this complex regulatory circuitry there is an additional pathway that ensures only a single Xist gene is expressed in XX cells, and that the single Xist allele in XY cells remains repressed. The classical model for this process invokes the presence of a blocking factor present at lim ...
Analysis of large and small colony L5178Y tk−/− mouse lymphoma
... Analysis of 122 spontaneous large and small colony mutants derived from L5178Y tk+l~ mouse lymphoma cells at 28 heteromorphic microsatellite loci on chromosome 11 showed that extensive loss of heterozygosity (LOH) is common in both large colony and small colony mutants, eliminating most chromosome 1 ...
... Analysis of 122 spontaneous large and small colony mutants derived from L5178Y tk+l~ mouse lymphoma cells at 28 heteromorphic microsatellite loci on chromosome 11 showed that extensive loss of heterozygosity (LOH) is common in both large colony and small colony mutants, eliminating most chromosome 1 ...
Human RIF1 and protein phosphatase 1 stimulate DNA replication
... Overall, the authors fill two gaps in the literature with this paper: 1. They confirm in humans much of which has been observed in yeast, which lends to RIF1's conserved and important role in genome regulation. 2. They observe a new role for RIF1 in origin stabilization, which adds another chapter t ...
... Overall, the authors fill two gaps in the literature with this paper: 1. They confirm in humans much of which has been observed in yeast, which lends to RIF1's conserved and important role in genome regulation. 2. They observe a new role for RIF1 in origin stabilization, which adds another chapter t ...
Persistence and Loss of Meiotic Recombination
... 2003). As predicted by the DSBR model, the less-active hotspot allele preferentially replaces the more-active one. The break-and-repair mechanism provides an elegant explanation for the link between biased gene conversion and crossing over but has the drawback of making the long-term maintenance of ...
... 2003). As predicted by the DSBR model, the less-active hotspot allele preferentially replaces the more-active one. The break-and-repair mechanism provides an elegant explanation for the link between biased gene conversion and crossing over but has the drawback of making the long-term maintenance of ...
Lethal Mutations and Balanced Lethal Systems in
... assumption that the frequency of lethals is the same for all chromosomes-an assumption unlikely to be fully justified-the lethal frequency for the whole genome was between 24 yoand 32 %. This agrees well with the 25 yo found by Kafer & Chen (1964) under similar conditions. Despite an intensive inves ...
... assumption that the frequency of lethals is the same for all chromosomes-an assumption unlikely to be fully justified-the lethal frequency for the whole genome was between 24 yoand 32 %. This agrees well with the 25 yo found by Kafer & Chen (1964) under similar conditions. Despite an intensive inves ...
The Nicotiana tabacum genome encodes two cytoplasmic
... (X58527), and represents the first thioredoxin h gene isolated from a higher plant. It encodes a polypeptide of 118 amino acids with the conserved thioredoxin active site Trp - Cys - Gly -Pro - Cys. This gene comprises two introns which have lengths of 1071 and 147 bp respectively, and three exons w ...
... (X58527), and represents the first thioredoxin h gene isolated from a higher plant. It encodes a polypeptide of 118 amino acids with the conserved thioredoxin active site Trp - Cys - Gly -Pro - Cys. This gene comprises two introns which have lengths of 1071 and 147 bp respectively, and three exons w ...
a method for detecting and typing of salmonella by multiplex pcr
... database GenBank (http://www.ncbi.nlm.nih.gov/genbank/) were analyzed. Multiple alignment of selected sequences, and their subsequent analysis to select PCR primers was performed using the computer program Bio Edit (v.7.2.4). The protocol of polymerase chain reaction has been developed on the basis ...
... database GenBank (http://www.ncbi.nlm.nih.gov/genbank/) were analyzed. Multiple alignment of selected sequences, and their subsequent analysis to select PCR primers was performed using the computer program Bio Edit (v.7.2.4). The protocol of polymerase chain reaction has been developed on the basis ...
Genetics Principles And Analysis
... Gene recombination in Escherichia coli Connection: Is a Bacteriophage an "Organism"? Alfred D. Hershey and Raquel Rotman 1948 Genetic recombination between host-range and plaque-type mutants of bacteriophage in single bacterial cells ...
... Gene recombination in Escherichia coli Connection: Is a Bacteriophage an "Organism"? Alfred D. Hershey and Raquel Rotman 1948 Genetic recombination between host-range and plaque-type mutants of bacteriophage in single bacterial cells ...