Homework - The Fenyo Lab
... The ChIP-seq tutorial typically uses the HPC cluster to process large data files. Requires a user account and a basic level of skill with Unix. The usual ChIP-seq informatics workflow is as follows: ...
... The ChIP-seq tutorial typically uses the HPC cluster to process large data files. Requires a user account and a basic level of skill with Unix. The usual ChIP-seq informatics workflow is as follows: ...
Lecture 21-23
... given gene) allows for efficiency and diversity. Consider: each gene contains about 20 times the number of base pairs necessary for a functional protein product (because of promoters, introns, etc). So if we can stick a few different proteins within the same coding region, we save a lot of space ove ...
... given gene) allows for efficiency and diversity. Consider: each gene contains about 20 times the number of base pairs necessary for a functional protein product (because of promoters, introns, etc). So if we can stick a few different proteins within the same coding region, we save a lot of space ove ...
The amount of DNA, # of genes and DNA per gene in various
... A pre-defined DNA sequence in the genome can be greatly amplified by repeated Polymerization cycles using 2 primers which hybridize to the ends of the target DNA. In each cycle, the amount of target DNA is doubled. After 10, 20 and 30 cycles, there is a 1000-, million- and billion-fold amplification ...
... A pre-defined DNA sequence in the genome can be greatly amplified by repeated Polymerization cycles using 2 primers which hybridize to the ends of the target DNA. In each cycle, the amount of target DNA is doubled. After 10, 20 and 30 cycles, there is a 1000-, million- and billion-fold amplification ...
Genome Sequence Quality - Rice Genome Annotation Project
... • Cot analysis of DNA was common in the 1970s to assess the repeat content in a given genome using a spectrophotometer and knowledge of reassociation kinetics • Principle is that highly repeated DNA will hybridize quickly to itself, moderately repeated DNA will take longer to reanneal and low comple ...
... • Cot analysis of DNA was common in the 1970s to assess the repeat content in a given genome using a spectrophotometer and knowledge of reassociation kinetics • Principle is that highly repeated DNA will hybridize quickly to itself, moderately repeated DNA will take longer to reanneal and low comple ...
DMD Reviews 101 - Action Duchenne
... Another brief review (3) stresses the importance of the correct diagnosis of DMD, noting not just the large mutations in the dystrophin gene but also any small ones. The authors remind us that the dystrophin gene is the largest known human gene and that 1/3 of mutations are new. These facts underlie ...
... Another brief review (3) stresses the importance of the correct diagnosis of DMD, noting not just the large mutations in the dystrophin gene but also any small ones. The authors remind us that the dystrophin gene is the largest known human gene and that 1/3 of mutations are new. These facts underlie ...
How do I identify codon numbers with the UCSC Genome Browser
... to navigate there. At the TP53 location we can see that there are multiple isoforms including a number of different start sites and in several locations, exons that are in one isoform and not another. The various gene sets have different numbers of isoforms and have various transcription or translat ...
... to navigate there. At the TP53 location we can see that there are multiple isoforms including a number of different start sites and in several locations, exons that are in one isoform and not another. The various gene sets have different numbers of isoforms and have various transcription or translat ...
A Long-Term Evolutionary Pressure on the Amount of Noncoding DNA
... is available on request. General Principles The simulated organisms have circular, double-strand binary genomes containing both coding and noncoding sequences (fig. 1). Each coding sequence encodes a ‘‘protein,’’ able to either activate or inhibit a number of functions. The phenotype is defined as t ...
... is available on request. General Principles The simulated organisms have circular, double-strand binary genomes containing both coding and noncoding sequences (fig. 1). Each coding sequence encodes a ‘‘protein,’’ able to either activate or inhibit a number of functions. The phenotype is defined as t ...
Ch. 13.1: BIOTECHNOLOGY
... Small, circular DNA Separate from chromosome. Contains a few genes. Make copies of itself Can be shared/ transferred b/w bacteria. ...
... Small, circular DNA Separate from chromosome. Contains a few genes. Make copies of itself Can be shared/ transferred b/w bacteria. ...
Directed Reading A
... ______ 1. What are chromosomes made of? a. inherited characteristics c. cells and structures b. generations d. protein and DNA ______ 2. What is the name of the material that determines inherited characteristics? a. deoxyribonucleic acid c. RNA b. ribosome d. amino acid ...
... ______ 1. What are chromosomes made of? a. inherited characteristics c. cells and structures b. generations d. protein and DNA ______ 2. What is the name of the material that determines inherited characteristics? a. deoxyribonucleic acid c. RNA b. ribosome d. amino acid ...
When is the gene not DNA? - Physicians and Scientists for Global
... molecule could be resynthesised generation after generation using the DNA molecule itself to guide the connections being formed between the units of a new molecule. In other words, the molecule fit our expectations of how biological information could be duplicated and passed on. This process was dub ...
... molecule could be resynthesised generation after generation using the DNA molecule itself to guide the connections being formed between the units of a new molecule. In other words, the molecule fit our expectations of how biological information could be duplicated and passed on. This process was dub ...
Ch. 5.1 Human Inheritance
... recessive gene on your X, you will have that recessive trait because the Y cannot offer a gene to compete! ...
... recessive gene on your X, you will have that recessive trait because the Y cannot offer a gene to compete! ...
DNA and Protein Synthesis Review Questions
... - Draw what would be seen after DNA is run through Gel Electrophoresis. Label which strands are the smallest and which are the largest. How did you determine this? ...
... - Draw what would be seen after DNA is run through Gel Electrophoresis. Label which strands are the smallest and which are the largest. How did you determine this? ...
File
... different genes from those of the bacterial chromosome. Plasmids can replicate independently of the main chromosomes of bacteria. The plasmid can be cleaved with restriction enzymes. If the plasmid and the foreign DNA have been cleaved with the same restriction enzyme, the sticky ends of each will m ...
... different genes from those of the bacterial chromosome. Plasmids can replicate independently of the main chromosomes of bacteria. The plasmid can be cleaved with restriction enzymes. If the plasmid and the foreign DNA have been cleaved with the same restriction enzyme, the sticky ends of each will m ...
Biotechnology Powerpoint
... A new DNA sequence created when the DNA of one organism is inserted into the DNA of another organism. This “new combination” of DNA is known as recombinant DNA. ...
... A new DNA sequence created when the DNA of one organism is inserted into the DNA of another organism. This “new combination” of DNA is known as recombinant DNA. ...
Genetics of Organelles III GENE330
... by mitochondrial defects, and in some cases, these defects are due to mutations in the mtDNA. One such disease is Leber’s hereditary optic neuropathy (LHON), a condition characterized by the sudden onset of blindness in adults. This disease is associated with the death of the optic nerve (at a physi ...
... by mitochondrial defects, and in some cases, these defects are due to mutations in the mtDNA. One such disease is Leber’s hereditary optic neuropathy (LHON), a condition characterized by the sudden onset of blindness in adults. This disease is associated with the death of the optic nerve (at a physi ...
Alignment of mRNA to genomic DNA Sequence
... then sequenced. The resulting EST sequences are compared with the nucleotide sequence of the entire genome (or the sequence of a single gene) to locate the gene (or parts of a gene) that contains each EST. ...
... then sequenced. The resulting EST sequences are compared with the nucleotide sequence of the entire genome (or the sequence of a single gene) to locate the gene (or parts of a gene) that contains each EST. ...
Quantitative Genetics and Whole Genome Approaches
... e. What have we learned about complex diseases from GWAS 1) Compared to the “good old days” before the ability to assess millions of SNPs, we now have discovered hundreds of loci linked to disease 2) These loci are primarily associated with a much reduced effect on the disease (low risk) and much hi ...
... e. What have we learned about complex diseases from GWAS 1) Compared to the “good old days” before the ability to assess millions of SNPs, we now have discovered hundreds of loci linked to disease 2) These loci are primarily associated with a much reduced effect on the disease (low risk) and much hi ...
Title goes here
... 1. Problems of metagenomic data (metagenomic data is the problem) (see IMG/M -> Using IMG/M -> About IMG/M -> Background for definitions) ...
... 1. Problems of metagenomic data (metagenomic data is the problem) (see IMG/M -> Using IMG/M -> About IMG/M -> Background for definitions) ...
Study Guide - Pierce College
... Putman/Pierce College Biol 160 09s Exam 4 Study Guide/20130426/Page 1 ...
... Putman/Pierce College Biol 160 09s Exam 4 Study Guide/20130426/Page 1 ...
Slide 1
... interested in getting the probability of getting exactly 8 annotation hits in the cluster but rather the probability of having 8 or more hits. In this case the probabilities of each of the possible matrices is summed. ...
... interested in getting the probability of getting exactly 8 annotation hits in the cluster but rather the probability of having 8 or more hits. In this case the probabilities of each of the possible matrices is summed. ...
Exploring the new world of the genome with DNA microarrays.
... nih.gov/Entrez/Genome/org.html). These genome sequences have not only made a new era of exploration imperative, but, providentially, they have also made it possible. DNA microarrays provide a simple and natural vehicle for exploring the genome in a way that is both systematic and comprehensive4–10. ...
... nih.gov/Entrez/Genome/org.html). These genome sequences have not only made a new era of exploration imperative, but, providentially, they have also made it possible. DNA microarrays provide a simple and natural vehicle for exploring the genome in a way that is both systematic and comprehensive4–10. ...
Human genome
The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. Human genomes include both protein-coding DNA genes and noncoding DNA. Haploid human genomes, which are contained in germ cells (the egg and sperm gamete cells created in the meiosis phase of sexual reproduction before fertilization creates a zygote) consist of three billion DNA base pairs, while diploid genomes (found in somatic cells) have twice the DNA content. While there are significant differences among the genomes of human individuals (on the order of 0.1%), these are considerably smaller than the differences between humans and their closest living relatives, the chimpanzees (approximately 4%) and bonobos. Humans share 50% of their DNA with bananas.The Human Genome Project produced the first complete sequences of individual human genomes, with the first draft sequence and initial analysis being published on February 12, 2001. The human genome was the first of all vertebrates to be completely sequenced. As of 2012, thousands of human genomes have been completely sequenced, and many more have been mapped at lower levels of resolution. The resulting data are used worldwide in biomedical science, anthropology, forensics and other branches of science. There is a widely held expectation that genomic studies will lead to advances in the diagnosis and treatment of diseases, and to new insights in many fields of biology, including human evolution.Although the sequence of the human genome has been (almost) completely determined by DNA sequencing, it is not yet fully understood. Most (though probably not all) genes have been identified by a combination of high throughput experimental and bioinformatics approaches, yet much work still needs to be done to further elucidate the biological functions of their protein and RNA products. Recent results suggest that most of the vast quantities of noncoding DNA within the genome have associated biochemical activities, including regulation of gene expression, organization of chromosome architecture, and signals controlling epigenetic inheritance.There are an estimated 20,000-25,000 human protein-coding genes. The estimate of the number of human genes has been repeatedly revised down from initial predictions of 100,000 or more as genome sequence quality and gene finding methods have improved, and could continue to drop further. Protein-coding sequences account for only a very small fraction of the genome (approximately 1.5%), and the rest is associated with non-coding RNA molecules, regulatory DNA sequences, LINEs, SINEs, introns, and sequences for which as yet no function has been elucidated.