SHORT COMMUNICATION One-step multiplex RT-PCR
... confers a diagnostic value for chronic myeloid leukaemia (CML). The aim of this study was to detect the presence of BCR/ABL fusion gene using a One-Step Multiplex Reverse Transcriptase –Polymerase Chain Reaction (RT-PCR) and identify the types of breakpoints in Malay patients diagnosed as CML. Their ...
... confers a diagnostic value for chronic myeloid leukaemia (CML). The aim of this study was to detect the presence of BCR/ABL fusion gene using a One-Step Multiplex Reverse Transcriptase –Polymerase Chain Reaction (RT-PCR) and identify the types of breakpoints in Malay patients diagnosed as CML. Their ...
The role of Cdx genes in the mammalian gut
... establishment as intestinal stem cells in the basal region of the mucosa whence they have migrated. This in turn might cause the overlying normal gastric mucosa to disappear in favour of the newly established intestinal phenotype. Another possibility is that the protein pump promoter used may be act ...
... establishment as intestinal stem cells in the basal region of the mucosa whence they have migrated. This in turn might cause the overlying normal gastric mucosa to disappear in favour of the newly established intestinal phenotype. Another possibility is that the protein pump promoter used may be act ...
Cytogenetic and Molecular Delineation of a Region of Chromosome
... Cytogenetic delineation of the commonly deleted segments. To determine the location of genes on 7q that may be involved in myeloid leukemogenesis, we first examined the breakpoints of the deletions in a consecutive series of 81 patients. This analysis included 26 patients with t-MDSI t-AMLand 55 pat ...
... Cytogenetic delineation of the commonly deleted segments. To determine the location of genes on 7q that may be involved in myeloid leukemogenesis, we first examined the breakpoints of the deletions in a consecutive series of 81 patients. This analysis included 26 patients with t-MDSI t-AMLand 55 pat ...
The Hematologist - American Society of Hematology
... interactive discussions in which they identified progressive and provocative strategies that address all aspects of SCD, including basic, translational, and clinical research, as well as access to care issues, both in North America and around the world. The discussions identified key partners, inclu ...
... interactive discussions in which they identified progressive and provocative strategies that address all aspects of SCD, including basic, translational, and clinical research, as well as access to care issues, both in North America and around the world. The discussions identified key partners, inclu ...
Gene Nomenclature System for Rice
... Genes with approved names and symbols will all be associated with a gene function or phenotype and, where possible, at the time of registration, researchers will be asked to identify a systematic locus identifier for the new gene from the RAP annotation database. Links to systematic_locus_IDs in oth ...
... Genes with approved names and symbols will all be associated with a gene function or phenotype and, where possible, at the time of registration, researchers will be asked to identify a systematic locus identifier for the new gene from the RAP annotation database. Links to systematic_locus_IDs in oth ...
Risk factors and treatment outcomes in osteomyelitis Alan D. Tice
... or reinfections over a period of 4 years. The incidence of recurrence peaked at 3 months at >6%. In fact, about half (56%) of the recurrences occurred by this time. Relapses occurred earlier than reinfections. Amputations or bone excisions were carried out in 27 cases. Of these procedures, 93% (25/2 ...
... or reinfections over a period of 4 years. The incidence of recurrence peaked at 3 months at >6%. In fact, about half (56%) of the recurrences occurred by this time. Relapses occurred earlier than reinfections. Amputations or bone excisions were carried out in 27 cases. Of these procedures, 93% (25/2 ...
Inferring gene-to-phenotype and gene-to
... reporters), need to be computationally excluded from consideration. For example, mice carrying an inducible knock-in of a mutant form of mouse Kcnj11 in the Gt(ROSA)26Sor locus and a transgene expressing cre recombinase in pancreatic cells, Tg(Ins2-cre)23Herr (genotype MGI:4430413), are annotated to ...
... reporters), need to be computationally excluded from consideration. For example, mice carrying an inducible knock-in of a mutant form of mouse Kcnj11 in the Gt(ROSA)26Sor locus and a transgene expressing cre recombinase in pancreatic cells, Tg(Ins2-cre)23Herr (genotype MGI:4430413), are annotated to ...
X inactivation Xplained
... Building a cis-limited repressive nuclear compartment The human Xi is comprised of a heterogeneous chromatin landscape [33]. Not all genes are repressed on the Xi and a relatively large set of genes escape from inactivation in human. Recently, a gene activity profile has been derived for the human X ...
... Building a cis-limited repressive nuclear compartment The human Xi is comprised of a heterogeneous chromatin landscape [33]. Not all genes are repressed on the Xi and a relatively large set of genes escape from inactivation in human. Recently, a gene activity profile has been derived for the human X ...
Making the Grade: Testing for Human Genetic Disorders
... possibility of a mistaken diagnosis raises questions regarding the distribution of the risks and benefits of an imperfect testing procedure. 9 Nevertheless, this Article assumes, for the purposes of this discussion, that the tests are flawless, since it is important to look at the ethical issues ari ...
... possibility of a mistaken diagnosis raises questions regarding the distribution of the risks and benefits of an imperfect testing procedure. 9 Nevertheless, this Article assumes, for the purposes of this discussion, that the tests are flawless, since it is important to look at the ethical issues ari ...
Chapter 10
... hedgehog, shrew, opposum, horse, elephant, pangolin, sloth, llama, and dolphin. Also sequenced are the genomes of many species of fruit flies, worms, and fungi, hundreds of bacteria, mitochondria, and chloroplasts, and thousands of viruses. Together these genomes represent a colossal amount of seque ...
... hedgehog, shrew, opposum, horse, elephant, pangolin, sloth, llama, and dolphin. Also sequenced are the genomes of many species of fruit flies, worms, and fungi, hundreds of bacteria, mitochondria, and chloroplasts, and thousands of viruses. Together these genomes represent a colossal amount of seque ...
Slide 1
... with their eyesight. They may be acquired to wear glasses or contact lenses to correct eye problems such as: nearsightedness, farsightedness and astigmatism. There is also sensitivity to light because there is not enough color in the iris to protect the retina. Aside from giving color to the hair, e ...
... with their eyesight. They may be acquired to wear glasses or contact lenses to correct eye problems such as: nearsightedness, farsightedness and astigmatism. There is also sensitivity to light because there is not enough color in the iris to protect the retina. Aside from giving color to the hair, e ...
Functional and ecological impacts of horizontal gene transfer in
... at the genomic level, and here HGT has emerged as a clear factor in the adaptation to various stresses. Nutritionally, two of the major limitations for marine microbes are iron and nitrogen [40]. Iron-stress especially has been the focus of recent attention, as it is known to be the limiting nutrien ...
... at the genomic level, and here HGT has emerged as a clear factor in the adaptation to various stresses. Nutritionally, two of the major limitations for marine microbes are iron and nitrogen [40]. Iron-stress especially has been the focus of recent attention, as it is known to be the limiting nutrien ...
E NEWS -
... of a family of enzymes that are common in bacteria, which use them to remodel their cell walls. Bacteriophages use the same enzymes to invade bacteria by chewing holes in their cell walls. In addition, the gene’s presence in an insect, the pea aphid, had previously been reported. But when they exami ...
... of a family of enzymes that are common in bacteria, which use them to remodel their cell walls. Bacteriophages use the same enzymes to invade bacteria by chewing holes in their cell walls. In addition, the gene’s presence in an insect, the pea aphid, had previously been reported. But when they exami ...
100 letí - originál
... sibling in each family was 98 or older; the others were at least 90. In those families, a stretch of DNA on Chromosome 4 stood out; another stretch, on Chromosome 2 was also a candidate, though not as strong. Now, Centagenetix will try to replicate the study with more subjects, and zero in on the ge ...
... sibling in each family was 98 or older; the others were at least 90. In those families, a stretch of DNA on Chromosome 4 stood out; another stretch, on Chromosome 2 was also a candidate, though not as strong. Now, Centagenetix will try to replicate the study with more subjects, and zero in on the ge ...
The Advantage and Application of Genetically Humanized Mouse
... testing. To overcome this limitation, mice expressing the human version of a particular drug target can be generated. The generation of mPGES-1 knock-In mice provides an excellent example of the use of humanized mice for drug efficacy and safety application27. Microsomal prostaglandin E synthase-1 m ...
... testing. To overcome this limitation, mice expressing the human version of a particular drug target can be generated. The generation of mPGES-1 knock-In mice provides an excellent example of the use of humanized mice for drug efficacy and safety application27. Microsomal prostaglandin E synthase-1 m ...
IVIg Therapy in PANDAS: Analysis of the Current Literature
... Our study had several limitations mainly associated with lack of uniformity within and among the published cases of PANDAS that precluded statistically meaningful comparisons. First, the dose, duration of treatment and timing of IVIg therapy was not uniform with some patients treated for one month a ...
... Our study had several limitations mainly associated with lack of uniformity within and among the published cases of PANDAS that precluded statistically meaningful comparisons. First, the dose, duration of treatment and timing of IVIg therapy was not uniform with some patients treated for one month a ...
Meet the Gene Machine
... family secrets involving paternity or adoption. If a baby tests positive for sickle-cell anaemia it means that both of his or her parents carry the mutation that causes it. Someone identified as carrying the gene may feel anger, while one who hasn’t inherited the condition may be overwhelmed by guil ...
... family secrets involving paternity or adoption. If a baby tests positive for sickle-cell anaemia it means that both of his or her parents carry the mutation that causes it. Someone identified as carrying the gene may feel anger, while one who hasn’t inherited the condition may be overwhelmed by guil ...
Hormone Replacement Therapy
... prescribed for off-label use, which constitutes about a fifth of all prescriptions. They are not approved by the FDA for such use, and yet it is well accepted that physicians should be able to use their discretion to prescribe medications for off-label use. ...
... prescribed for off-label use, which constitutes about a fifth of all prescriptions. They are not approved by the FDA for such use, and yet it is well accepted that physicians should be able to use their discretion to prescribe medications for off-label use. ...
p53 as a target for anti-cancer drug development
... 393 amino acids in human (Fig. 1). It has five structural and functional domains: an N-terminal transactivation domain, a proline-rich regulatory domain, a sequence-specific DNAbinding domain, an oligomerization domain and a C-terminal domain involved in the regulation of DNA-binding. In terms of th ...
... 393 amino acids in human (Fig. 1). It has five structural and functional domains: an N-terminal transactivation domain, a proline-rich regulatory domain, a sequence-specific DNAbinding domain, an oligomerization domain and a C-terminal domain involved in the regulation of DNA-binding. In terms of th ...
Respiratory Epithelial Gene Expression in Patients with Mild and
... Despite having identical cystic fibrosis transmembrane conductance regulator genotypes, individuals with ⌬F508 homozygous cystic fibrosis (CF) demonstrate significant variability in severity of pulmonary disease. This investigation used high-density oligonucleotide microarray analysis of nasal respi ...
... Despite having identical cystic fibrosis transmembrane conductance regulator genotypes, individuals with ⌬F508 homozygous cystic fibrosis (CF) demonstrate significant variability in severity of pulmonary disease. This investigation used high-density oligonucleotide microarray analysis of nasal respi ...
Quantitative and Single-Gene Perspectives on the Study of Behavior
... Genetic Dissection: Circadian Rhythms in Drosophila The clearest contribution single-gene mutants have made is in the realm of identifying individual genes that are central to behavioral mechanisms, pointing the way to the unraveling of the cellular mechanisms. Perhaps the best example of this strat ...
... Genetic Dissection: Circadian Rhythms in Drosophila The clearest contribution single-gene mutants have made is in the realm of identifying individual genes that are central to behavioral mechanisms, pointing the way to the unraveling of the cellular mechanisms. Perhaps the best example of this strat ...
Laboratory Diagnosis and Treatment of Hyperadrenocorticism in
... B. Insulin requirements may change rapidly. Owner should monitor urine samples 2-3 times a day for glucose. With negative results, insulin dose is lowered 25% at next injection. C. Cortisol causes insulin antagonism so with resolution of hyperadrenocorticism: 1. Insulin requirements will decrease. ...
... B. Insulin requirements may change rapidly. Owner should monitor urine samples 2-3 times a day for glucose. With negative results, insulin dose is lowered 25% at next injection. C. Cortisol causes insulin antagonism so with resolution of hyperadrenocorticism: 1. Insulin requirements will decrease. ...
A novel de novo frame-shift mutation of the EDA gene in a
... the maxilla) (Sekiguchi et al. 2005), suggesting phenotypic heterogeneity of this syndrome. It is interesting to note that heterozygous mutation carriers of XLHED may have variable clinical features, displaying minor or moderate degrees of hypodontia, hypotrichosis, and hypohidrosis. In some familie ...
... the maxilla) (Sekiguchi et al. 2005), suggesting phenotypic heterogeneity of this syndrome. It is interesting to note that heterozygous mutation carriers of XLHED may have variable clinical features, displaying minor or moderate degrees of hypodontia, hypotrichosis, and hypohidrosis. In some familie ...
Biomarkers for Epithelial Ovarian Cancers
... vs rest signature. In this gure, the solid line represents the accuracy of classi cation with respect to the rst 30 genes obtained by our method from the original 7; 069 genes. The dashed line represents the LOOCV accuracy with respect to the rst 30 of the 73 genes provided by [17]. Notice that w ...
... vs rest signature. In this gure, the solid line represents the accuracy of classi cation with respect to the rst 30 genes obtained by our method from the original 7; 069 genes. The dashed line represents the LOOCV accuracy with respect to the rst 30 of the 73 genes provided by [17]. Notice that w ...
Genetic Testing of Inherited Cardiac Disorders
... including Sydney, Melbourne and Brisbane and these comprise a multidisciplinary team of cardiologists, clinical geneticists and genetic counsellors/coordinators[10]. Some DNA testing is performed within Australian laboratories, however testing for many conditions requires sending samples to overseas ...
... including Sydney, Melbourne and Brisbane and these comprise a multidisciplinary team of cardiologists, clinical geneticists and genetic counsellors/coordinators[10]. Some DNA testing is performed within Australian laboratories, however testing for many conditions requires sending samples to overseas ...
Gene therapy
Gene therapy is the therapeutic delivery of nucleic acid polymers into a patient's cells as a drug to treat disease. Gene therapy could be a way to fix a genetic problem at its source. The polymers are either expressed as proteins, interfere with protein expression, or possibly correct genetic mutations.The most common form uses DNA that encodes a functional, therapeutic gene to replace a mutated gene. The polymer molecule is packaged within a ""vector"", which carries the molecule inside cells.Gene therapy was conceptualized in 1972, by authors who urged caution before commencing human gene therapy studies. By the late 1980s the technology had already been extensively used on animals, and the first genetic modification of a living human occurred on a trial basis in May 1989 , and the first gene therapy experiment approved by the US Food and Drug Administration (FDA) occurred on September 14, 1990, when Ashanti DeSilva was treated for ADA-SCID. By January 2014, some 2,000 clinical trials had been conducted or approved.Early clinical failures led to dismissals of gene therapy. Clinical successes since 2006 regained researchers' attention, although as of 2014, it was still largely an experimental technique. These include treatment of retinal disease Leber's congenital amaurosis, X-linked SCID, ADA-SCID, adrenoleukodystrophy, chronic lymphocytic leukemia (CLL), acute lymphocytic leukemia (ALL), multiple myeloma, haemophilia and Parkinson's disease. Between 2013 and April 2014, US companies invested over $600 million in the field.The first commercial gene therapy, Gendicine, was approved in China in 2003 for the treatment of certain cancers. In 2011 Neovasculgen was registered in Russia as the first-in-class gene-therapy drug for treatment of peripheral artery disease, including critical limb ischemia.In 2012 Glybera, a treatment for a rare inherited disorder, became the first treatment to be approved for clinical use in either Europe or the United States after its endorsement by the European Commission.