Study of Alternative Functions of the Mitochondrial Protein Bak
... Figure 2: Mitochondrial electron transport chain and ROS production. Mitochondrial complex I uses electrons carried by NADH and complex II uses electrons from FADH 2 formed by succinate dehydrogenase to reduce coenzyme Q. Coenzyme Q shuttles these electrons to complex III, where they are transferred ...
... Figure 2: Mitochondrial electron transport chain and ROS production. Mitochondrial complex I uses electrons carried by NADH and complex II uses electrons from FADH 2 formed by succinate dehydrogenase to reduce coenzyme Q. Coenzyme Q shuttles these electrons to complex III, where they are transferred ...
Identification of Two Mammalian Reductases
... equivalent of yeast Elo2p (8). Ssc1 and Ssc2 (sequence similarity to Cig30 1 and 2) subsequently were identified based on homology to Cig30 (9). Ssc1 is the functional equivalent of Elo3p in yeast (9). Definitive fatty acid substrate specificity has not been assigned to Ssc2, although two of its sub ...
... equivalent of yeast Elo2p (8). Ssc1 and Ssc2 (sequence similarity to Cig30 1 and 2) subsequently were identified based on homology to Cig30 (9). Ssc1 is the functional equivalent of Elo3p in yeast (9). Definitive fatty acid substrate specificity has not been assigned to Ssc2, although two of its sub ...
Supplementary document Trehalose/2
... Trehalose/2-sulfotrehalose biosynthesis and glycine-betaine uptake are widely spread mechanisms for osmoadaptation in the Halobacteriales ...
... Trehalose/2-sulfotrehalose biosynthesis and glycine-betaine uptake are widely spread mechanisms for osmoadaptation in the Halobacteriales ...
9-Amino Acids and Proteins
... and let them cool. When they are cool, add 10% NaOH to each tube dropwise until the solutions are basic. (You can do this by adding one drop of NaOH, mixing well with a stirring rod, and touching the stirring rod to a piece of red litmus paper. If the litmus paper does not turn blue, add another dro ...
... and let them cool. When they are cool, add 10% NaOH to each tube dropwise until the solutions are basic. (You can do this by adding one drop of NaOH, mixing well with a stirring rod, and touching the stirring rod to a piece of red litmus paper. If the litmus paper does not turn blue, add another dro ...
protein, glutathione, essential oils, energy, weight loss
... The most active glutathiones are complexes with selenium. The first glutathione genes were produced when bacteria were exposed to oxygen, long before human life. It forms our most important antioxidant system, and mostly makes up for primates’ loss of ability to make vitamin C. However, lack of redu ...
... The most active glutathiones are complexes with selenium. The first glutathione genes were produced when bacteria were exposed to oxygen, long before human life. It forms our most important antioxidant system, and mostly makes up for primates’ loss of ability to make vitamin C. However, lack of redu ...
- Cal State LA - Instructional Web Server
... Entrez integrates the scientific literature, DNA and protein sequence databases, 3D protein structure and protein domain data, and other information into a tightly interlinked system. ...
... Entrez integrates the scientific literature, DNA and protein sequence databases, 3D protein structure and protein domain data, and other information into a tightly interlinked system. ...
Turnover-based in vitro selection and evolution of biocatalysts from
... ranging from 10–2 to 10–6 M. Binding of candidate scFvs to the reactive substrate was also analyzed by BIAcore18. Monitored in real time, covalent binding is indicated by very slow association to the chip, because only a fraction of the molecules are trapped after turnover. Covalently trapped molecu ...
... ranging from 10–2 to 10–6 M. Binding of candidate scFvs to the reactive substrate was also analyzed by BIAcore18. Monitored in real time, covalent binding is indicated by very slow association to the chip, because only a fraction of the molecules are trapped after turnover. Covalently trapped molecu ...
P site - Industrial ISD
... • A point mutation that results in replacement of a pair of complimentary nucleotides with another nucleotide pair is called a base-pair substitution. • Some base-pair substitutions have little or no impact on protein function. • In silent mutations, alterations of nucleotides still indicate the sa ...
... • A point mutation that results in replacement of a pair of complimentary nucleotides with another nucleotide pair is called a base-pair substitution. • Some base-pair substitutions have little or no impact on protein function. • In silent mutations, alterations of nucleotides still indicate the sa ...
Metazoan Remaining Genes for Essential Amino Acid Biosynthesis
... amino acid supplements [1]. This means that pathways for de novo amino acid biosynthesis are missing in their genomes, characterizing the Essential Amino Acid (EAA) phenotype. There is no consensus over the exact number of essential amino acids, but it is normally accepted that His, Ile, Leu, Lys, M ...
... amino acid supplements [1]. This means that pathways for de novo amino acid biosynthesis are missing in their genomes, characterizing the Essential Amino Acid (EAA) phenotype. There is no consensus over the exact number of essential amino acids, but it is normally accepted that His, Ile, Leu, Lys, M ...
Characterisation of the novel proteins expressed in corn line 1507
... Corn (Zea mays L.) has undergone substantial genetic breeding by conventional methods over many centuries of cultivation and has been safely consumed as food and feed for thousands of years. Products derived from corn include highly processed corn grain fractions such as flour, high fructose corn sy ...
... Corn (Zea mays L.) has undergone substantial genetic breeding by conventional methods over many centuries of cultivation and has been safely consumed as food and feed for thousands of years. Products derived from corn include highly processed corn grain fractions such as flour, high fructose corn sy ...
A Simple and Sensitive Detection of OmpA Protein from Escherichia... Crystal Microbalance Jung-Chih Chen , S. Sadhasivam
... proposed. Fig. 3 step (a) show the thiolated crystals were activated with EDC-NHS and the first decrease in frequency was noted at 25 min which indicated the formation of active NHS ester on the crystal surface, then wash out by PBS (step (b)). When OmpA antibody was injected into the flow cell, sub ...
... proposed. Fig. 3 step (a) show the thiolated crystals were activated with EDC-NHS and the first decrease in frequency was noted at 25 min which indicated the formation of active NHS ester on the crystal surface, then wash out by PBS (step (b)). When OmpA antibody was injected into the flow cell, sub ...
Sequence and Tissue Distribution of a Second Protein of Hepatic
... isolated rat and mouse liver gap junction fractions (Nicholson et al., 1987). The sequence also shows extensive homology throughout its length with two previously cloned junetional proteins (Cx32 and Cx43). Alignment of the coding region of Cx26 with Cx32 (Paul, 1986) requires the insertion of a gap ...
... isolated rat and mouse liver gap junction fractions (Nicholson et al., 1987). The sequence also shows extensive homology throughout its length with two previously cloned junetional proteins (Cx32 and Cx43). Alignment of the coding region of Cx26 with Cx32 (Paul, 1986) requires the insertion of a gap ...
Rapid increase of cytosolic content of acetyl-CoA
... N-terminal 200 amino acids of ACC-β has no homology at all with the first N-terminal 70 amino acids of ACC-α whereas the remaining region containing all known functional domains shares approximately 70% identity. The molecular difference arises from N-terminal region of ACC. In addition, N-terminus ...
... N-terminal 200 amino acids of ACC-β has no homology at all with the first N-terminal 70 amino acids of ACC-α whereas the remaining region containing all known functional domains shares approximately 70% identity. The molecular difference arises from N-terminal region of ACC. In addition, N-terminus ...
ScrFl restriction/modification system from
... GATC 3' sites and shows significant identity with its isoschizomers DpnII (31YO)and MboI (34 O/O ;Moineau et al., 1995) but has relatively little homology with other ENases. While the majority of type I1 ENases do not have any obvious homology at the primary sequence level, X-ray crystallography stu ...
... GATC 3' sites and shows significant identity with its isoschizomers DpnII (31YO)and MboI (34 O/O ;Moineau et al., 1995) but has relatively little homology with other ENases. While the majority of type I1 ENases do not have any obvious homology at the primary sequence level, X-ray crystallography stu ...
N-terminus of M2 protein could induce antibodies with inhibitory
... New influenza vaccines have been designed based on the fact that the extracellular domain of M2 protein (M2e) is nearly invariant in all influenza A strains. To clarify which exact region of M2e could induce antibodies with inhibitory activities against influenza virus replication, four overlapping ...
... New influenza vaccines have been designed based on the fact that the extracellular domain of M2 protein (M2e) is nearly invariant in all influenza A strains. To clarify which exact region of M2e could induce antibodies with inhibitory activities against influenza virus replication, four overlapping ...
SEP PyMOL Guide
... PyMOL is also capable of making view changes to parts of the protein structure and not all of it. To do this however it needs to know what part you want to look at or change. For that, you need to know about selections. Making selections requires typing commands. Although the commands are relatively ...
... PyMOL is also capable of making view changes to parts of the protein structure and not all of it. To do this however it needs to know what part you want to look at or change. For that, you need to know about selections. Making selections requires typing commands. Although the commands are relatively ...
INDIGO-BINDING DOMAINS IN CELLULASE MOLECULES
... Gusakov A.V., Sinitsyn A.P., Berlin A.G., Popova N.N., Markov A.V., Okunev O.N., Tikhomirov D.F., and Emalfarb M. Appl. Biochem. Biotechnol. 1998. 75. P. 279. Van Tilbeurgh H., Tomme P., Claeyssens M., Bhikhabhai R., and Pettersson G. FEBS Lett. 1986. 204. P. 223. Kastelyanos O.F., Ermolova O.V., Si ...
... Gusakov A.V., Sinitsyn A.P., Berlin A.G., Popova N.N., Markov A.V., Okunev O.N., Tikhomirov D.F., and Emalfarb M. Appl. Biochem. Biotechnol. 1998. 75. P. 279. Van Tilbeurgh H., Tomme P., Claeyssens M., Bhikhabhai R., and Pettersson G. FEBS Lett. 1986. 204. P. 223. Kastelyanos O.F., Ermolova O.V., Si ...
Lecture 21: Structure of Prokaryotic Cells
... bacterial plasmid to perform the function of vector are as follows1. Origin of replication-Like any other replicating DNA, plasmid DNA needs its own independent origin of replication to provide replication start site to make more copies. It decides the range of bacterial host strain can be use with ...
... bacterial plasmid to perform the function of vector are as follows1. Origin of replication-Like any other replicating DNA, plasmid DNA needs its own independent origin of replication to provide replication start site to make more copies. It decides the range of bacterial host strain can be use with ...
Evidence for the absence of amino acid isomerization in microwave
... Samples of UHT milk and reconstituted formulas were heated under conditions corresponding to those applied a t home for warming up or cooking food (600 W for 3 min), as well as a t a lower power (70 W) for a prolonged time (20min). The latter conditions were applied as an attempt to distinguish an i ...
... Samples of UHT milk and reconstituted formulas were heated under conditions corresponding to those applied a t home for warming up or cooking food (600 W for 3 min), as well as a t a lower power (70 W) for a prolonged time (20min). The latter conditions were applied as an attempt to distinguish an i ...
Activity 3
... 6. What do you think would happen if you reduced the incubation time to 30 minutes? How did this affect optical density results? • Reducing the incubation time to 30 minutes reduced the optical density due to the reduced amount of time for digestion of BAPNA. ...
... 6. What do you think would happen if you reduced the incubation time to 30 minutes? How did this affect optical density results? • Reducing the incubation time to 30 minutes reduced the optical density due to the reduced amount of time for digestion of BAPNA. ...
Protein structure and function
... molecules essential to life, whereas immunoglobulins fight infectious bacteria and viruses. 5- Many hormones are proteins or peptides. Protein hormones include insulin, prolactin and LH. Important peptide hormones include glucagon, ADH and calcitonin. ...
... molecules essential to life, whereas immunoglobulins fight infectious bacteria and viruses. 5- Many hormones are proteins or peptides. Protein hormones include insulin, prolactin and LH. Important peptide hormones include glucagon, ADH and calcitonin. ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.