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Proteases - Home - KSU Faculty Member websites
Proteases - Home - KSU Faculty Member websites

... If the HIV-1 protease can be selectively inhibited, then new HIV particles cannot form Several novel protease inhibitors are currently marketed as AIDS drugs Many such inhibitors work in a culture dish However, a successful drug must be able to kill the virus in a human subject without blocking othe ...
Glyceraldehyde-3-Phosphate Dehydrogenase Is a GABAA Receptor
Glyceraldehyde-3-Phosphate Dehydrogenase Is a GABAA Receptor

c Syun-Ru Yeh‡ and Denis L. Rousseau
c Syun-Ru Yeh‡ and Denis L. Rousseau

... may form deep kinetic traps during folding and have been reported to be linked to human pathogenesis (10, 11). To unify the kinetic studies from a large variety of proteins, a “new view” of protein folding has been postulated in which unique folding channels are replaced by a statistical distributio ...
Safety assessment - Federal Register of Legislation
Safety assessment - Federal Register of Legislation

... 88017 (MON 88017) in Australia and New Zealand is agreed on the basis of the available scientific evidence for the following reasons: ...
From Sequence to Structure
From Sequence to Structure

... Replication of the AIDS virus HIV depends on the action of a protein-cleaving enzyme called HIV protease. This enzyme is the target for protease-inhibitor drugs (shown in grey). (PDB 1a8k) ...


... spend high expenditure for importing Glycine max (soya beans) because it is the most popular and main protein source for vegetarians as well as animal feed. The search for an alternative source of plant protein is important to cut down the expenditure, reduce protein energy malnutrition and to maint ...
Fatty acid and phospholipid metabolism in prokaryotes
Fatty acid and phospholipid metabolism in prokaryotes

ARF-Aux/IAA interactions through domain III/IV are not strictly
ARF-Aux/IAA interactions through domain III/IV are not strictly

... DNA target sites or through interactions with other transcription factors or chromatin-associated proteins. Potential involvement of chromatin modifications in negatively regulating auxin response gene expression is suggested from studies with TPL, which likely plays a role in inducing a repressive ...
Evolution of the enzymes of the citric acid cycle and the
Evolution of the enzymes of the citric acid cycle and the

Gluconeogenesis Glycogen metabolism
Gluconeogenesis Glycogen metabolism

... unless they are near chain ends or branch points. The products are then maltose, maltotriose and a mixture of small branched fragments (with 5 - 9 glucose residues) called α-dextrins. Those products are hydrolysed to free glucose by the action of both maltase and saccharase-isomaltase, bound in the ...
Mechanisms underlying the essential role of mitochondrial
Mechanisms underlying the essential role of mitochondrial

... Oxidative Medicine and Cellular Longevity Another aspect of lipid metabolism and transport known to define longevity of chronologically aging yeast is the abundance of triacylglycerols (TAGs) [67–70]. These so-called neutral lipids are synthesized in the endoplasmic reticulum (ER) and then deposited ...
Identification of the Human Erythrocyte Glucose Transporter (GLUT1
Identification of the Human Erythrocyte Glucose Transporter (GLUT1

... facilitative glucose transporters selectively pump ...
Three-dimensional Structure of Goose
Three-dimensional Structure of Goose

dna sequence analysis: pairwise comparison
dna sequence analysis: pairwise comparison

... BestFit uses the local homology algorithm of Smith and Waterman (Advances in Applied Mathematics 2; 482-489 (1981)) to find the best segment of similarity between two sequences. BestFit reads a scoring matrix that contains values for every possible GCG symbol match. The program uses these values to ...
PhoB by Phosphate Stress and Controlled by
PhoB by Phosphate Stress and Controlled by

... in response to Pi stress (52). Identification of the interrupted ackA gene in mutant strain RmMSU4 was accomplished by cloning the transposon and flanking DNA from RmMSU4 (previously designated psi25 [52]), sequencing the transposon-chromosome junctions, and then conducting homology searches of majo ...
Metabolism of Macromolecules in Bacteria Treated
Metabolism of Macromolecules in Bacteria Treated

... Mixing the two virginiamycin components potentiated their antibiotic activity about 200-fold. In fact, a level of 0.5 pg./ml. of factors M and S induced an inhibitory effect comparable to that of IOO pg. of a single component. The numbers of colony-forming units after incubation for different period ...
Substrate Specificity and Mechanism from the Structure of
Substrate Specificity and Mechanism from the Structure of

... bacterial sepsis. Galactokinase deficiency (galactosemia II; MIM 230200) in man is an inborn error of galactose metabolism and is linked to development of cataracts during the first months of life and also pre-senile cataracts, the onset of which is between 20 and 50 years of age.3 Over 20 mutations ...
Report Organelles in Blastocystis that Blur the
Report Organelles in Blastocystis that Blur the

... same organelles is more suggestive of hydrogenosomal metabolism. To clarify the metabolic properties of the Blastocystis MLOs, we used 3330 clusters constructed from our EST data for a comparative BLAST search against the yeast and human mitochondrial proteomes [14, 15]. In addition, the KEGG Automa ...
Sample pages 2 PDF
Sample pages 2 PDF

... scenario, the pathway developed over a long time period, where it probably showed many variations, but when the chemical reactions fitted neatly together, it was fixed by selective pressure. No organism could tinker with alternative pathways when living on carbohydrates. They could only refine its r ...
Sequence Analysis Tools
Sequence Analysis Tools

... – The presence of a gap is ascribed more significance than the length of the gap • A single mutational event may cause the insertion or deletion of more than ...
Aromatic Amino Acids-Guanidinium Complexes through
Aromatic Amino Acids-Guanidinium Complexes through

... In order to study the effect that guanidinium complexation has on the aromaticity of the amino acids in all the systems considered, we have calculated the NICS values at 0, 1 and 2 Å over the ring center of each aromatic system. Some authors prefer the use of NICS(zz) [also called NICS(out-of-plane) ...
Aromatic Amino Acids-Guanidinium Complexes through
Aromatic Amino Acids-Guanidinium Complexes through

... In order to study the effect that guanidinium complexation has on the aromaticity of the amino acids in all the systems considered, we have calculated the NICS values at 0, 1 and 2 Å over the ring center of each aromatic system. Some authors prefer the use of NICS(zz) [also called NICS(out-of-plane) ...
Structure, mechanism and function of prenyltransferases
Structure, mechanism and function of prenyltransferases

... mutagenesis study in which Y81 was systematically replaced with each of the other 19 amino-acid residues showed that Y81A, Y81G and Y81S are capable of making hexaprenyl pyrophosphate (C30) [59]. The final products of Y81C, Y81H, Y81I, Y81L, Y81N, Y81T and Y81V are C25 geranylfarnesyl pyrophosphate. ...
View - BioOne
View - BioOne

... Jianying Shen, Antonio DiTommaso, Mingquan Shen, Wei Lu, and Zhengming Li* Nitrogen-fixing cyanobacteria are vital photosynthetic microorganisms that contribute to soil fertility by fixing atmospheric nitrogen and are also important for maintaining ecosystem stability. These microorganisms can be ve ...
WHAT_CHECK report for 1BFX
WHAT_CHECK report for 1BFX

... Residue. Residues/atoms in tables are normally given in a few parts: • A number. This is the internal sequence number of the residue used by WHAT IF. The first residues in the file get number 1, 2, etc. • The residue type. Normally this is a three letter amino acid type. • The sequence number, betwe ...
< 1 ... 37 38 39 40 41 42 43 44 45 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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