• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Elsevier Scientific Publishing Company, Amsterdam
Elsevier Scientific Publishing Company, Amsterdam

... The role of acyl chain length and unsaturation in bilayers with strong negative charge was also examined (Fig. 5). In this case the activity was largely independent on the chain length and unsaturation. Maximal reactivation of ATPase complex occurred at amounts of phospholipids lower than the amount ...
Nitrate reductase activity in chicory roots
Nitrate reductase activity in chicory roots

... Detopping plantlets abolishes the shoot-to-root relationships. In the absence of sucrose, nitrate reduction is rapidly decreased (Brouquissee/o/., 1992). This resembles senescence in leaves. The decrease of enzymatic activities related to nitrogen assimilation and the subsequent increases of protein ...
Isolated Spinach Ribulose-1,5-Bisphosphate
Isolated Spinach Ribulose-1,5-Bisphosphate

... “The relationship betWeen the trimethylation of lysine 77 and cytochrome c metabolism in Saccharomyces cerevisiae, ” Int. J. Biochem. 26(5):721—734 (1994); Cessay et al., “Further investigations regarding the role of trimeth yllysine for cytochrome c uptake into mitochondria,” Int. J. Biochem. 23(7, ...
File
File

... With reference to Table 4.2, and the information in (iii), state the cause or causes of reduced fertility in these mutant worms at each temperature. ...
Phylogeny of Firmicutes with special reference to Mycoplasma
Phylogeny of Firmicutes with special reference to Mycoplasma

The relevance of carbon dioxide metabolism in
The relevance of carbon dioxide metabolism in

The Primary Structure of the Calcium Ion
The Primary Structure of the Calcium Ion

Renin Precursor Synthesis and Renin
Renin Precursor Synthesis and Renin

... Proteins are synthesized in the cytoplasma of the cell by the ribosomes, which use RNA as a messenger for the amino acid sequence. Ribosomes from one species and messenger RNA from another can interact and produce a protein with the correct structure. This is used in the so-called cellfree translati ...
Biochemical and Biophysical Research Communications
Biochemical and Biophysical Research Communications

... ularly antagonistic activity against Candida albicans and Saccharomyces cerevisiae. Fusaricidins also have excellent germicidal activity to gram-positive bacteria such as Staphylococcus aureus [3,4]. In addition, they have antifungal activity against Leptosphaeria maculans, which causes black root r ...
Single-Amino Acid Substitutions Alter the Specificity and Affinity of
Single-Amino Acid Substitutions Alter the Specificity and Affinity of

No Slide Title
No Slide Title

... ubiquitin-tagged intracellular proteins takes place in the proteasomes of all cells. ...
Chemistry 1010
Chemistry 1010

... – 9000 different proteins in a cell – Individual human being >100,000 different – Fibrous Protein • Insoluble in H2O • Used mainly for structural purposes ...
Purification, Characterization, and Amino Acid
Purification, Characterization, and Amino Acid

... the proteins were reduced and pyridylethylated. By automated Edman degradation, the first 48 residues of acanthin II were identified unequivocally with a repetitive yield of 93%. The remaining sequence was determined by treatment with BNPS-skatole, which cleaves at the C-terminal side of tryptophan ...
PDF
PDF

... also be used for the production of the bio-based biodegradable polymer polylactic acid (Benninga, 1990; Datta et al., 1995). The pH sensitivity and limited ability to synthesize B-vitamins and amino acids of these lactobacilli, increases the cost of lactic acid production due to the requirement of c ...
Electron Transfer Chain
Electron Transfer Chain

... Transmembrane H+ flux associated with this reaction will be discussed in the section on oxidative phosphorylation. An atomic-level structure is not yet available for the entire complex I, which in mammals includes at least 46 proteins, along with prosthetic groups FMN & several Fe-S centers. ...
Text - Enlighten: Publications
Text - Enlighten: Publications

... protein [64]. Structural studies on the domain have revealed that it contains two amphipathic -helices (Helix I and Helix II) that are separated by a non-structured segment with several hydrophobic residues, termed the hydrophobic loop (HL) [63]. Removal of segments from D2 that includes deletion o ...
Protein digestion and amino acid absorption along
Protein digestion and amino acid absorption along

... ileum of cod. If the protein content in minced saithe fillet is assumed to be 60 % (86 % of the diet), the extent of hydrolysis can be estimated approximately. In rainbow trout fed a casein diet, the peptide fraction of the pyloric caeca region contains a maximum of 89 % of total amino acids (Dabrow ...
The tyrosine regulated DAHP synthase and the biosynthetic
The tyrosine regulated DAHP synthase and the biosynthetic

... ARO4, that can be feedback-inhibited by phenylalanine and tyrosine, respectively. Crystal structures of the phenylalanine regulated DAHP synthase of Escherichia coli and of the tyrosine inhibitable isoenzyme of S. cerevisiae are available. With the help of amino acid substitutions at the catalytic c ...
Scientific Opinion on Dietary Reference Values for protein
Scientific Opinion on Dietary Reference Values for protein

... 0.66 g protein/kg body weight per day based on a meta-analysis of nitrogen balance data as the average requirement (AR). In healthy adults, the protein requirement per kg body weight is considered to be the same for both sexes and for all body weights. Considering the 97.5th percentile of the popula ...
The first true obligately syntrophic propionate
The first true obligately syntrophic propionate

... were inoculated with 0?2 g freeze-dried granular sludge. After addition of 20 mM sodium propionate and 10 % (v/v) of a H2/CO2-grown culture of Methanospirillum hungatei JF-1T, the enrichment culture was incubated at 37 uC. After 3 months, the propionate was depleted and CH4 was produced. Acetate was ...
Gepard: a rapid and sensitive tool for creating dotplots on genome
Gepard: a rapid and sensitive tool for creating dotplots on genome

... A novel functionality of the Gepard project is a client–server dotplot mode. The server provides all publicly available genomes including functional annotations of the genes. This feature allows the user to rapidly compare different genomes interactively without need to download any data manually. ...
BLAST Homepage and Selected Search Pages
BLAST Homepage and Selected Search Pages

Neural Marker Antibodies Epitope Tag Antibodies
Neural Marker Antibodies Epitope Tag Antibodies

... The metabotropic glutamate receptors (mGluR’s) represent a gene family of G-protein coupled receptors. These receptors all bind the amino acid glutamate – the major excitatory neurotransmitter of the nervous system – but differ in their primary amino acid structure, their pharmacology, and their dis ...
Euglena gracilis Rhodoquinone:Ubiquinone Ratio and
Euglena gracilis Rhodoquinone:Ubiquinone Ratio and

... subsequent analysis by two-dimensional PAGE was cultured as described previously (6). Euglena cultures for determination of UQ9 and RQ9 were grown in a BIOSTAT B 10-liter fermenter (Braun Biotech) at a culturing volume of 7 liters with light continuously at 5000 lux, constant temperature at 28 °C, a ...
Proteases - Home - KSU Faculty Member websites
Proteases - Home - KSU Faculty Member websites

... If the HIV-1 protease can be selectively inhibited, then new HIV particles cannot form Several novel protease inhibitors are currently marketed as AIDS drugs Many such inhibitors work in a culture dish However, a successful drug must be able to kill the virus in a human subject without blocking othe ...
< 1 ... 36 37 38 39 40 41 42 43 44 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report