• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Hemoglobin - Huntingdon College
Hemoglobin - Huntingdon College

... combination, bind antigenic peptides presented on major histocompatibility complex (MHC) class II molecules on antigen-presenting cells. Helper T cells make cytokines and perform other functions that help coordinate the immune response. • CD8+ cytotoxic T cells: T cells displaying co-receptor CD8 ar ...
Gene Regulation -
Gene Regulation -

... affect regulation. This potentially confusing affect is avoided by using IPTG, an artificial inducer that can enter the cell and bind repressor protein without functional Z or Y. Some mutations in Z and Y have a polar effect. For example, a frameshift in Z will also mean that Y and A won't be made s ...
Gel electrophoresis
Gel electrophoresis

... covalent bonds to each other. The specific information of the nucleic acid is coded in the unique sequence of the four kinds of nucleotides present in the chain. DNA is composed of two nucleotide chains entwined around each other in a double helix. The base sequence of nucleic acids can be determine ...
Bio 263/F94/T2
Bio 263/F94/T2

... b. interaction between phospholipid R groups c. the interaction between adjacent or nearly adjacent regions of the nucleic acid backbone d. the primary structure e. c and d 22. With which of the following methods are S units associated as a unit of measure? a. gel filtration b. ultracentrifugation c ...
file1
file1

... - only use residues in above formula that have a positive Z-score - to reduce noise, restrict to values that have above average positive relative entropy ...
Protein Synthesis
Protein Synthesis

... Ribosomes are made up of 2 parts. When joined there is a small space between them where the mRNA attaches and slides through. This is also the area where the tRNAs attach and for the polypeptide chain. ...
Rabbit anti-Occludin (N-term)
Rabbit anti-Occludin (N-term)

... The 65 kDa occludin protein was first identified in chicken using monoclonal antibodies.1,2 The chicken occludin cDNA was subsequently cloned, with the amino acid sequence revealing that the protein is organized into five distinct domains: a short N-terminal cytoplasmic domain (domain A), two extrac ...
1. The formation of a peptide bond between two amino acids is an
1. The formation of a peptide bond between two amino acids is an

... C) Protein 4 is the protein that shows the greatest overall homology to protein 1. D) Proteins 2 and 3 show a greater evolutionary distance than proteins 1 and 4. E) The portions of amino acid sequence shown suggest that these proteins are completely unrelated. 10. The three-dimensional structure of ...
The six elements that make up 99.9% of all living things include
The six elements that make up 99.9% of all living things include

... down reactions and prevent overheating of the cells 5. they usually speed up chemical reactions ...
How Enzymes Work
How Enzymes Work

... some cases by shielding the had persistent structure and that catalytic site from contact with Elucidating the active site. In the crystal structure of a lysozyme mutant bound to destruction of that structure could a synthetic sugar substrate, the sugar ring in the active site is distorted, and the ...
A steroid/thyroid hormone receptor superfamily member in
A steroid/thyroid hormone receptor superfamily member in

... includes the two cysteine-cysteine zinc fingers that comprlse a DNA-bindlng domaln which typifies all members of the superfamily. ln addition, three regions (Regions lI-IV) in the carboxy-termlnal portion of the protein that encode the putative hormone-binding domain of the 2C gene product resemble ...
nucleic acids
nucleic acids

... As the protein is being made, the first couple amino acids act as a tag to determine where the protein will end up. When the protein is released from the ribosome if needed, it will be taken to the ER and/or golgi for folding, processing, tagging and packaging ...
Review on Biochemistry: Protein Chemistry
Review on Biochemistry: Protein Chemistry

...  -carboxyglutamate: found in prothrombin and certain Ca2+-binding protein.  Desmosine (a derivative of four Lys residues): found in the fibrous protein elastin.  Selenocysteine: Selenium replaces sulfur in cysteine during amino acid synthesis (derived from serine).  Amino acids not as constitue ...
Mass Spectrometry - University of Exeter
Mass Spectrometry - University of Exeter

Reorientation (AMA-1)
Reorientation (AMA-1)

... • Parasite Entry – reorganization of submembrane cytoskeleton – PVM formation – shedding of merozoite surface proteins (eg, MSP-1) ...
DNA/RNA/Protein Questions
DNA/RNA/Protein Questions

... What does Translation mean? What organelle makes proteins? What role does tRNA play in making proteins? What is an "anticodon"? What structure is it on? How is mRNA used to make proteins. Why are proteins so important to life? Given a strand of mRNA, be able to make an amino acid chain. (You will be ...
Mass spectrometry - Justin Benesch
Mass spectrometry - Justin Benesch

... • Separation of ions according to their ability to traverse a region of gas under the influence of a weak electric field • Separation is based on ion ‘mobility’, unlike time-of-flight separation (mass) ...
ces-1 (cG-17): sc-12177 - Santa Cruz Biotechnology
ces-1 (cG-17): sc-12177 - Santa Cruz Biotechnology

... Several proteins involved in regulating and executing programmed cell death have been identified in C. elegans. CED-2, which is similar to the human adaptor protein CrkII, as well as CED-5 and CED-7, which are orthologs of the mammalian DOCK180 and ABC transporter proteins, respectively, are involve ...
CSM 101 Fall 2010 Timeline
CSM 101 Fall 2010 Timeline

... leaves the receptor, diffuses along the membrane, and then binds to an enzyme, altering the enzyme’s shape and activity. Once activated, the enzyme can trigger the next step leading to a cellular response. Binding of signaling molecules is reversible. The activating changes in the GPCR, as well as t ...
Gene Section MAPK4 (mitogen-activated protein kinase 4) Atlas of Genetics and Cytogenetics
Gene Section MAPK4 (mitogen-activated protein kinase 4) Atlas of Genetics and Cytogenetics

... kinases. The human ERK4 protein is made of 587 amino acids and contains a typical kinase domain located at the N-terminal extremity. Another region with homology to the MAP kinase ERK3 (C34 domain) has been identified after the kinase domain. The function of the C34 domain is unknown. ...
Food Chemistry for 1..
Food Chemistry for 1..

Bis2A 9.0 Introduction to Gene Regulation
Bis2A 9.0 Introduction to Gene Regulation

... response to changes in cellular or external factors. This biology is important for understanding how cells adjust changing environments, including how some cells decide to become specialized for certain functions (e.g. tissues) in a multicellular organism. ...
mnw2yr_lec1_2004
mnw2yr_lec1_2004

... • There are about 3bn (3  109) nucleotides in the nucleus of almost all of the trillions (3.5  1012 ) of cells of a human body (an exception is, for example, red blood cells which have no nucleus and therefore no DNA) – a total of ~1022 nucleotides! • Many DNA regions code for proteins, and are ca ...
Document
Document

... An approach to predict a model of the three-dimensional structure of a given protein sequence (TARGET) based on an alignment to one or more known protein structures (TEMPLATES) The homology modeling method is based on the assumption that the structure of an unknown protein is similar to known struct ...
Biochemistry Webquest
Biochemistry Webquest

...  This causes _________in the carbon chain.  These compounds are called _________ because they have fewer than the maximum number of _________.  Fats with the maximum number of _________ are called _________.  Draw and label the two different types of fats _________ are structurally similar to fa ...
< 1 ... 387 388 389 390 391 392 393 394 395 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report