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How do proteins recognize DNA
How do proteins recognize DNA

B2 Protein structure and function
B2 Protein structure and function

... • Computational methods will allow the prediction of both structure and possible function from simple amino acid sequence information. • Understanding of the true function of a protein still requires its isolation and biochemical and structure characterization. • Identification of all the other prot ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

... II State whether the following statements are True or False ...
Biomolecules Worksheet
Biomolecules Worksheet

... 3). There are 3 main groups of amino acids in cells: non-polar, polar, charged. Explain what you would look for in an amino acid to identify it as one of these types. ...
• Double helix -- twisted ladder shape of DNA, like spiral staircase
• Double helix -- twisted ladder shape of DNA, like spiral staircase

... “rung”? hydrogen Why is this a good bond for this location? allows DNA to unzip easily ...
Macromolecule Scramble
Macromolecule Scramble

... (blood or phloem)  metabolic roles  Ex: enzymes in all organisms, plasma proteins and antibodies in mammals  Fibrous  form long fibres  mostly consist of repeated sequences of amino acids which are insoluble in water  usually have structural roles  Ex.  Collagen in bone and cartilage  Kerat ...
Macromolecules - Essentials Education
Macromolecules - Essentials Education

... poypeptides usually make up a protein. They can also code for the production of an RNA molecule.   ...
Intro-Cell-Physiology
Intro-Cell-Physiology

... • Transfer RNA (tRNA) - small, between 70 and 80 nucleotides, cloverleaf-shaped molecules that “transfer” amino acid molecules to the mRNA. ...
Prescott`s Microbiology, 9th Edition Chapter 18 – Microbial
Prescott`s Microbiology, 9th Edition Chapter 18 – Microbial

... Figure 18.4 Why is it important that the DNA to be sequenced is immobilized in all three of these techniques? With a flow cell, unless the DNA was attached to the substrate, it would flow away. Figure 18.6 Which step (or steps) in this process is (are) replaced by PCR amplification and immobilizatio ...
slides
slides

WHAT`S A CARBOHYDRATE
WHAT`S A CARBOHYDRATE

Proteins and Electrophoresis
Proteins and Electrophoresis

... • The –R groups determine, for the most part, the properties of the protein • Proteins rich in Asp, Glu are acidic (albumin is an example) • Post-translational modifications of ...
Membranes
Membranes

... o (i.e. a selective barrier – controls concentration variation of molecules in cell) ...
Laboratory of Dr. Wayne L. Hubbell Protocol designed by Carlos J
Laboratory of Dr. Wayne L. Hubbell Protocol designed by Carlos J

... acetate, 0.5 M NaCl, pH 4.0) buffers to remove any traces of ethylenediamine that may be nonspecifically bound to the beads. For long-term storage, store the resin in 20% ethanol. For functionalization with DBCO, pre-equilibrate the amino-Sepharose beads with PBS (pH 7.2), then add a freshly prepar ...
Biomolecules PPT
Biomolecules PPT

... other hormones controlling human sexual development ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... mechanisms, including the production of substances with phytohormonal activity or antimicrobial substances such as antibiotics (13, 15, 21). The production of HCN in pseudomonads has been reported (5), for example, but it has also been reported in other bacteria. In contrast to plants, cyanogenesis ...
Bioinformatics in Biochemistry, small
Bioinformatics in Biochemistry, small

... A. Does the mouse protein have all of the highlighted residues that you know to be important to the structure and/or function of human hemoglobin? Mark/highlight the locations of these residues in the mouse protein on the printout of your BLAST results. B. What can you conclude about the importance ...
Macromolecule Review (PP)
Macromolecule Review (PP)

... Function: Provide structure for tissues and organs, allow muscles to contract, transport oxygen, and make up enzymes which carry out chemical reactions. ...
1.3.6 Structural Role of Biomolecules
1.3.6 Structural Role of Biomolecules

... other hormones controlling human sexual development ...
Powerpoint on Proteins
Powerpoint on Proteins

... • The signal consists of a peptide: a sequence of amino acids in a particular order that form an integral part of the protein. • Specific amino acid sequences (topogenic signals) determine whether a protein will pass through a membrane into a particular organelle, become integrated into the membrane ...
translation
translation

...  TRANSLATION: In the ribosome, tRNAs match up with their codons in the mRNA.  The backsides of the tRNAs have specific amino acids attached to them. When the tRNAs line up, the amino acids bond to each other and let go of the tRNA.  The chain of amino acids is called a protein.  The protein then ...
What Do I already know about Prehistoric Cultures?
What Do I already know about Prehistoric Cultures?

... • most plant foods contain limited amounts of one or two amino acids • for this reason single-item diets, such as those made up almost solely of corn or yams, can lead to protein deficiency The Cultural Feast, 2nd Ed., p. 52 ...
Marvelous Macromolecules - Pregitzersninjascienceclasses
Marvelous Macromolecules - Pregitzersninjascienceclasses

... Collagen – three polypeptides coiled like a rope – good for structure Hemoglobin – four polypeptide (two different types) – carries oxygen ...
0c5168dab2ecd61778b5bb175973dab5 UNPDF
0c5168dab2ecd61778b5bb175973dab5 UNPDF

... 10. The significance of “directionality” of the monomers in a polymer is that when you put the monomers together in a certain sequence/order they have ______________________ a. The process of “putting monomers together” is called b. What is lost during the process ? c. What kind of bond is formed ge ...
2. Propensity
2. Propensity

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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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