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Lab 8 - Electrophoresis
Lab 8 - Electrophoresis

... polypeptide chain is called the secondary structure of the protein. The secondary structures of proteins are stabilized by hydrogen bonds in which hydrogen serves as a bridge between oxygen and nitrogen atoms (-C=O HN-). A common secondary structure is the -helix that consists of a single polypepti ...
A dietary supplement is intended to provide nutrients that may
A dietary supplement is intended to provide nutrients that may

... branched-chain amino acids or individual amino acids (such as glutamine). Amino acids are considered to be inferior to whole protein and have been used by some companies to artificially inflate and falsify protein values in their product (protein spiking). Many protein supplements explicitly indicat ...
Developing a Novel Means of Observing the
Developing a Novel Means of Observing the

Biochemistry_of_Cells abridged
Biochemistry_of_Cells abridged

Dr Gisela Storz Biosketch
Dr Gisela Storz Biosketch

... Institute  of  Child  Health  and  Human   Development  in  Bethesda,  where  she  is  a  Senior  Investigator.    Dr.   Storz  has  made  contributions  in  multiple  fields  of  molecular  biology,   including  groundbreaking  experiments ...
Chapter 02 The Molecules of Life
Chapter 02 The Molecules of Life

... energetically favorable form that it will assume. However, for other proteins, a particular sequence may have several forms that are just as energetically favorable as another. These proteins require a helper molecule, known as a chaperone, to help it fold correctly. ...
Life Without Water: Expression of Plant LEA Genes - The Keep
Life Without Water: Expression of Plant LEA Genes - The Keep

... Fig. 1. (A) mRNA expression profiles for the LEA genes Afrlea1 and Afrlea2 from Artemia franciscana embryos. LEA mRNAs are maintained 7–14-fold higher in the two desiccation-tolerant embryonic stages (i.e., diapause and post-diapause) compared to the desiccation-intolerant nauplius larva that served ...
Novel domains and orthologues of eukaryotic
Novel domains and orthologues of eukaryotic

... hindered by the nucleosome, a structure containing DNA wrapped around two copies each of histones H2A, H2B, H3 and H4. Disruption of the structural integrity of the nucleosome, by histone acetylation and/or methylation, by DNA unwinding, or by histone translocation, allows passage of the RNAP-II com ...
Dietary Protein Quality: Its importance in Nutrition and Health
Dietary Protein Quality: Its importance in Nutrition and Health

... In particular vegetable-based proteins are of lower quality than dairy based proteins ...
Fueling First-Class Athletes
Fueling First-Class Athletes

... The hours that athletes spend in practices, training, and competing place large demands on the physical conditioning of players. The peak energy level and power needed to compete in any sport can be maximized with a balanced whole-foods diet focused on high-quality protein, healthy fats, and whole-g ...
NMR-driven secondary and tertiary structure model of Ca
NMR-driven secondary and tertiary structure model of Ca

... residues, which, in other EF-hand proteins, are known to interact with basic residues on the target [16]. Lastly, the fingerprint region within EF-III contains one or more Met residues, which may also be important in target interaction and allow multiple targets to interact with calmodulin [17]. It i ...
Small G-protein
Small G-protein

... Monomeric G-proteins are activated by proteins which induce a conformational change resulting in reduced affinity to GDP, and thus in GDP release. The general term for such proteins is GEF (guanine nucleotide exchange factor). The GEFs of the small G-proteins are not the activated receptors, but rat ...
printed handout sheet
printed handout sheet

Improved insights into protein thermal stability: from the
Improved insights into protein thermal stability: from the

... The idea developed in [18, 7] consists in taking advantage of the bias of the potentials towards the dataset from which they are extracted. We constructed a set of about 200 proteins with known melting temperature and 3-dimensional (3D) structure and divided it into different subsets according to th ...
Protein Modeling Challenge Science Olympiad Trial Event
Protein Modeling Challenge Science Olympiad Trial Event

... RasMol is a great program and may be continued to be used by Science Olympiad Teams. However, since there have not been updates to RasMol to allow for it to operate easily on Macs, Jmol will be the computer visualization program to be used in the future. The commands that you have used in RasMol are ...
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... pharmacokinetics and pharmacological effects of drugs. It is one of the most important areas of investigation in drug pharmacokinetics research. Drug binding to plasma proteins was first considered to be a nonspecific physical phenomenon, similar to the adsorption of small molecules to charcoal. Acc ...
B2 Protein structure
B2 Protein structure

... b-sheet: hydrogen bonding of the peptide bond N-H and C=O groups to the complementary groups of another section of the polypeptide chain x ...
Coronavirus JHM: a Virion-assoeiated Protein Kinase
Coronavirus JHM: a Virion-assoeiated Protein Kinase

... cells suggested that the enzymic activity responsible was a consequence of virus infection and by analogy to many other viruses m a y be associated with released virion particles. We therefore assayed purified virus for in vitro kinase activity and Fig. 3 demonstrates that there is indeed a virion-a ...
CHAPTER 5 CARBON CONTENT: LOW LARGE HYDROPHOBIC
CHAPTER 5 CARBON CONTENT: LOW LARGE HYDROPHOBIC

... The genetic code plots the relationship between a triplet base sequence on RNA and an amino acid that corresponds to a protein associated with a required function in organisms. Proteins are considered as the major functional units in all living organisms. They evolve from mRNAs with post-translation ...
Science Take-Out: From DNA to Protein Structure and Function
Science Take-Out: From DNA to Protein Structure and Function

... Each DNA molecule consists of many genes. Each gene carries coded information for how to make one type of protein. Your body makes many different kinds of proteins. Every protein in your body has a specific shape. It is this specific shape that allows each protein to perform a specific job. The comb ...
Digestion of Proteins
Digestion of Proteins

CH 3: The Molecules of Life
CH 3: The Molecules of Life

... Order of amino acids in a protein Bonding: peptide bonds between amino acids • Peptide bonds are _________ bonds ...
Pa I I, hl.  L.   Blasticidin-S: on... Cycloheximide  has been used widely  as  ...
Pa I I, hl. L. Blasticidin-S: on... Cycloheximide has been used widely as ...

... 2 . In conjunction with the spindle, specialized regions (spindle plaques) on the external surface of the nwleus act as termini for opposite poles of the spindle and may be involved in the polymerization ond orientation of the forming spindle fibers. 3. A dense granule-spindle plaque complex ob=rved ...
Protein Similarities II
Protein Similarities II

... The "lighter" amino acids aren't necessary to the electron-carrying function; at least, they don't have to be precisely the same for the protein to work. This isn't at all unlikely. For instance, there is an alpha helix going up the left side of the image above. It probably has to be an alpha helix ...
HA Tagged Yeast Clones and Collection - Technical
HA Tagged Yeast Clones and Collection - Technical

... The HA insertion provides a means to study the function of yeast genes in a large-scale fashion by analysis of conditional phenotypes. The generation of conditional alleles and hypomorphic mutants that exhibit partial gene function is especially important for the analysis of essential genes2,3. It h ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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