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Use the illustration below to answer
Use the illustration below to answer

... When RNA is produced from a DNA template, there is a specific region just before the actual start of RNA production. What is this region called, and what is the process called? a. Initiation region; translation b. Promoter; transcription c. Promoter; translation d. Initiation; RNA processing ...
Characterization of the IEll0 Gene of Herpes Simplex Virus Type 1
Characterization of the IEll0 Gene of Herpes Simplex Virus Type 1

... to an Sstl site), pJR3 and p110 are similar except that p110 contains only a single BamHI site, while pJR3 has an additional BamHI site in the vector sequences, p175, used in later experiments, was derived from pGX58 and contained the coding sequences of the IE175 gene under the control of the simia ...
Comparative genomics and metabolic reconstruction of
Comparative genomics and metabolic reconstruction of

... – L36, L33, L31, S14 are the only ribosomal proteins duplicated in more than one species – L36, L33, L31, S14 are four out of seven ribosomal proteins that contain the zinc-ribbon motif (four cysteines) – Out of two (or more) copies of the L36, L33, L31, S14 proteins, one usually contains zinc-ribbo ...
Introduction to Spectroscopy and Fluorescence
Introduction to Spectroscopy and Fluorescence

... protein hydrophobic cores. The aromatic side chain can also mean that Tryptophan is involved in stacking interactions with other aromatic sidechains. Role in function: As it contains a non-carbon atom (nitrogen) in the aromatic ring system, Tryptophan is more reactive than Phenylalanine though it is ...
Comparison of Trypsin Immobilization Techniques With or Without a
Comparison of Trypsin Immobilization Techniques With or Without a

Oxidative Phosphorylation - Study in Universal Science College
Oxidative Phosphorylation - Study in Universal Science College

... is responsible for the final combination of reducing equivalents with molecular oxygen. • It has a high affinity for O2 thus allowing the respiratory chain to function at its maximum. • This is the only irreversible reaction in the chain and hence provides direction to the movement of reducing equiv ...
A Simple Lattice Model That Captures Protein Folding, Aggregation
A Simple Lattice Model That Captures Protein Folding, Aggregation

... The ability of many proteins to convert from their functional soluble state to amyloid fibrils can be attributed to intermolecular beta strand formation. Such amyloid formation is associated with neurodegenerative disorders like Alzheimer’s and Parkinson’s. Molecular modelling can play a key role in ...
PowerPoint-Präsentation
PowerPoint-Präsentation

... noise“(variations of other cell components such as RNA polymerase) Idea for an experiment: Gene for CFP (green fluorescent protein) und YFP (yellow fluorescent protein, shown in red) are controlled by the same promotor, hence the mean concentration of CFP and YFP is equal => Expression probability s ...
Lecture 1 - Health Computing: Pitt CPATH Project
Lecture 1 - Health Computing: Pitt CPATH Project

... Chromosomes • A chromosome is a long and tightly wound DNA string (visible under a microscope) • Chromosomes can be linear or circular • Prokaryotes usually have a single chromosome, often a circular DNA molecule ...
Amino Acids, Proteins and Enzymes
Amino Acids, Proteins and Enzymes

... (COOH, NH3, etc.) but different spatial arrangement (like cis-trans) ...
Sample pages 2 PDF
Sample pages 2 PDF

... structure elements and interactions between side chains of the amino acids. An assembly of several polypeptide chains into one large protein molecule is called the quaternary structure. Hemoglobin was the first oligomeric protein for which the complete tertiary and quaternary structures were determi ...
Biochemistry
Biochemistry

... Biochemistry and Molecular Biology is a required course for basic Medicine. It can be defined as the science to clarify the phenomenon and the nature of life and to explain the biochemistry problems about normal human and disease processes at the molecular level. Biochemistry and medicine are closel ...
10 CODON ANTI- CODON CYTOPLASM RIBOSOME tRNA AMINO
10 CODON ANTI- CODON CYTOPLASM RIBOSOME tRNA AMINO

... Protein synthesis is the process of making proteins. The DNA contains the codes to make the proteins, but it CANNOT leave the nucleus. As a result, it must deliver the message in a different way. STEP 1 OF PROTEIN SYNTEHSIS-TRANSCRIPTION. The section of the DNA that contains the code for the needed ...
A CHASE domain containing protein kinase OsCRL4
A CHASE domain containing protein kinase OsCRL4

... acids long, have drawn special at tention, as they are the putative recognition sites for cytokinin. CRE1/AHK4 mutation of Thr 278 to Ile in this domain leads to a loss of function (Mahonen et al., 2000). Sequence comparison showed the presence of this domain in other receptor-like protein in both p ...
The Strategic Use of Ruminally Protected Amino Acids in Dairy
The Strategic Use of Ruminally Protected Amino Acids in Dairy

... percentage (Bucholtz et al., 2008). In another experiment with similar results (Benefield et al., 2006) it appeared that because of higher than expected DMI, estimated gram requirements were in excess, and this may have been the reason for the failure, but it certainly was not the result of low MET ...
Heterologous products from the yeast Kluyveromyces lactis
Heterologous products from the yeast Kluyveromyces lactis

... process operations and costs of possible industrial productions. These advantageous perspectives make heterologous protein production a common practice of applied research. However, drawbacks are often encountered in developing new production systems, depending on a large variety of problems and inc ...
Effect of
Effect of

... through a filter. Ammonia is produced in fish as an end product of protein catabolism and is also generated in the aquatic system by the breakdown of uneaten feed and waste. Ammonia is soluble in water, and minimal amounts are lost through evaporation. Even low levels of ammonia can be toxic to gill ...
IJEB 48(4) 373-377
IJEB 48(4) 373-377

... The diet rich in riboflavin increased the induction period of liver tumours by azo-dyes21. Similary, rats fed with both riboflavin and cupric oxyacetate hexahydrate delayed the formation of liver tumour induced by azo-dyes18. The results of the present study also indicate that supplementation of vit ...
Bchm2000_P1 - U of L Class Index
Bchm2000_P1 - U of L Class Index

Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry

... IV (Merck, Germany) was applied to determine protein subunit molecular masses in kDa: cytochrome C (12), myoglobin (16), carboanhydrase (30), ovalbumin (42), albumin (66), and ovotransferrin (78). The MWs of unknown proteins were estimated by SDS-PAGE by plotting the log of the MW of standard protei ...
Chem 465 Biochemistry II Hour Exam 2
Chem 465 Biochemistry II Hour Exam 2

... Essay questions - Answer any 5. 1. In the respiratory chain there are 5 types of compounds used to transport electrons. What are these five electron carriers, and how are they similar or different from each other? NADH Nicotinamide adenine dinucleotide. Nicotinic acid attached to an adenine nucleoti ...
Projection Structure of a Plant Vacuole Membrane Aquaporin by
Projection Structure of a Plant Vacuole Membrane Aquaporin by

... a-TIP was routinely obtained. Mature, ungerminated seeds were used so that the puri®ed a-TIP would be at a physiological ground state of phosphorylation (Johnson & Chrispeels, 1992). When obtained from dry seed, a-TIP eluted as a single broad peak by anion-exchange chromatography (Figure 1(c)). By S ...
Protein Measurement with the Folin Phenol Reagent
Protein Measurement with the Folin Phenol Reagent

Molecules - Chapter 2
Molecules - Chapter 2

... acids. They are molecules with diverse structures and functions.  Each different type of protein has a characteristic amino acid composition and order.  Proteins range in size from a few amino acids to thousands of them.  Folding is crucial to the function of a protein and is influenced largely b ...
Amino Acid Composition of Enzymatically Hydrolysed Potato Protein
Amino Acid Composition of Enzymatically Hydrolysed Potato Protein

... was heated at 85°C for 15 min, was conducted in a reaction vessel. After cooling down, the hydrolysates were centrifuged for 15 min with a Rotofix 32A by Hettich at 3000 g. The supernatants containing hydrolysed potato protein were freeze-dried and stored at –25°C for further analysis. Moreover, a c ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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