• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Structural Prediction of Membrane
Structural Prediction of Membrane

... refinement with the exceptions of the peak for the fourth helix which was not as high (vide infiu) and the correlation with the ...
Osmoadaptative Strategy and Its Molecular
Osmoadaptative Strategy and Its Molecular

... stable intracellular K+ content that is 17 higher than E. coli, but 0.3–0.6 that of Halobacterium salinarum (Youssef et al. 2014). As expected, Hala. paucihalophilus possesses a highly acidic proteome. More puzzling, however, are the cases of the Halanaerobiales Halanaerobium praevalens, “Halanaer ...
Higher plant cellulose synthases | Genome Biology | Full Text
Higher plant cellulose synthases | Genome Biology | Full Text

... All cellulose synthases described to date have a number of conserved structural features. It is thought that CesA acts as a member of a protein complex that can be visualized by electron microscopy on the surface of the plasma membrane in structures called ‘rosettes’. These appear to consist of six ...
Carbon-Based Molecules
Carbon-Based Molecules

... chemical energy in organisms. Animal fats are found in foods such as meat and butter. You know plant fats as oils, such as olive oil and peanut oil. The structures of fats and oils are similar. They both consist of a molecule called glycerol (glihs-uh-rawl) bonded to molecules called fatty acids. Fa ...
Sports Nutrition for Beginners Basic Eating Tips for Active People
Sports Nutrition for Beginners Basic Eating Tips for Active People

... are appropriate guidelines. Recommended Dietary Allowance (RDA)- adequate intake level for ~ 98% of healthy people. Adequate Intake (AI)- value estimated when RDA is unable to be determined. ...
Computational  Biophysics
Computational  Biophysics

... Conformational stability is a critical property of the collagen triple helix. It is in part ensured by the presence of a repetitive GXY sequence motif, with a high frequency of proline residues in the X position and hydroxyproline residues in the Y position. A high natural abundance of lysine residu ...
PPT - Hirst Group - The University of Nottingham
PPT - Hirst Group - The University of Nottingham

... Predicts dihedral angles from various amino acid properties amino acid composition and predicted structure. ...
A Superfamily of S Locus-Related Sequences in
A Superfamily of S Locus-Related Sequences in

... The expected results agree well with the actual results except for the 8.8-kb EcoRI fragment, which is unaccounted for in our collection of clones. Our screens of genomic libraries failed to identify additional S-related genes that might correspond to this EcoRI fragment. Because the genomic DMA pre ...
FoldSynth: Interactive 2D/3D Visualisation Platform for Molecular
FoldSynth: Interactive 2D/3D Visualisation Platform for Molecular



... Choice C: Pick any super-secondary structure. Describe, or sketch, its structure and briefly discuss the intramolecular forces that stabilize it. β-α-β an alpha helix placed on top of a two stranded β-sheet (2 pts) H-bonds would stabilize the individual secondary structures. (2 pts) The sheet would ...
Iron-sulfur proteins and the role of iron in the gene expression of
Iron-sulfur proteins and the role of iron in the gene expression of

Slide 1
Slide 1

... Neuronal/synaptic signaling is mediated by neurotransmitters released at the interface between the signaling and the target cell, called synapse. The release of neurotransmitters at the synapse is controlled from the cell body through electrical signals. Neurotransmitters bind cell surface receptors ...
03_Lecture_Presentation - Cornerstone Charter Academy
03_Lecture_Presentation - Cornerstone Charter Academy

...  Two polynucleotide strands wrap around each other to form a DNA double helix – The two strands are associated because particular bases always hydrogen bond to one another ...
Localization of Low-sulfur Keratin Proteins in the Wool Follicle Using
Localization of Low-sulfur Keratin Proteins in the Wool Follicle Using

... proteins, the low-sulfur proteins, and that the matrix is composed of a mixture of proteins designated the high-sulfur and high-tyrosine proteins (13). Cross-linking by disulfide bridges stabilizes the keratin structures and renders the proteins insoluble (14). Although much of the earlier biochemic ...
MedicalBiochemistry
MedicalBiochemistry

Diapositiva 1
Diapositiva 1

...  Besides introducing carbonyl groups into the protein, ROS are also responsible for deamidation, racemization and isomerization of residues.  Gln and Asn residues deamidate and racemize about their C alpha atoms to the Disomers.  Asymmetric side chains of Thr and Ile residues convert from the L-i ...
Experimentally testing the hypothesis of a limited amino acid
Experimentally testing the hypothesis of a limited amino acid

... gradually increased up to 20 through the course of evolution. If it was the case, how many number of and which types of amino acids were involved in the earliest protein synthesis system? By comparing homologous amino acid sequences from different organisms or referring to various criteria for amino ...
Fast procedure for reconstruction of full
Fast procedure for reconstruction of full

... To efficiently sample protein-conformational space, protein structure prediction algorithms often use a reduced protein representation. For example, instead of calculating interactions between all protein atoms, every amino acid can be represented by a single center of interaction (e.g., positioned o ...
How Enzymes Are Named - Our biological products and solutions
How Enzymes Are Named - Our biological products and solutions

Initiation, elongation, and termination strategies in polyketide and
Initiation, elongation, and termination strategies in polyketide and

Poster
Poster

... of chains of amino acids bonded together. Molecules called tRNA (transfer RNA) carry single amino acids to a ribosome, where these amino acids get attached to one another in a sequence based on the genetic information in the mRNA (messenger RNA). ...
Ribosome Display: In Vitro Selection of Protein
Ribosome Display: In Vitro Selection of Protein

... To accelerate the procedure of bringing a library into the ribosome-display format, we generated a vector containing the necessary flanking regions (ribosome-display vector, pRDV; Fig. 2) . The library is PCR amplified, cut with the appropriate restriction enzymes, and ligated into the vector such t ...
THE IDENTIFICATION AND CHARACTERISATION OF THE
THE IDENTIFICATION AND CHARACTERISATION OF THE

... The sequences of clones A-18 and A-66 were identical and determined by the NRPSpredictor 2 to be specific for phenylalanine. Protein BLAST analysis of the amino acid sequence of both clones showed a high similarity to an amino acid adenylation protein in Granuliella mallensis MP5ACIX8 (YP_00507340.1 ...
Myoglobin from equine skeletal muscle (M0630)
Myoglobin from equine skeletal muscle (M0630)

... helical regions, joined by non-ordered or random coil regions. These 8 helices (A, B, C, D, E, F, G, H) are folded back on top of one another, and the heme is situated between helices E and F. The heme is almost totally buried. Only the edge carrying the two hydrophylic propionic acid groups is expo ...
PLANT PROTEIN PHOSPHATASES
PLANT PROTEIN PHOSPHATASES

... one or more regulatory subunits. Genetic studies have shown yeast PP1 catalytic subunit genes to be essential for cellular processes as diverse as glycogen accumulation, mitosis, and translational control (118). Regulatory subunits define specific functions of PP1 catalytic activity in vivo by contr ...
< 1 ... 121 122 123 124 125 126 127 128 129 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report