Purification and Some: Characteristics of a Monomeric Racemase
... thermostability of the enzyme exceeded that from B. stearothermophilus. The alanine racemase from T. thermophilus HB8 showed no loss of activity even after being incubated for 30min at 8O”C, while the enzyme from B. stearothermophilus only retained about 40% of its original activity under similar co ...
... thermostability of the enzyme exceeded that from B. stearothermophilus. The alanine racemase from T. thermophilus HB8 showed no loss of activity even after being incubated for 30min at 8O”C, while the enzyme from B. stearothermophilus only retained about 40% of its original activity under similar co ...
Lecture 6 - Andrew.cmu.edu
... last three residues (Ala-Phe-Leu )could not be determined due to limitation in the Edman degradation to 7 steps, these are highlighted gray). Ala ...
... last three residues (Ala-Phe-Leu )could not be determined due to limitation in the Edman degradation to 7 steps, these are highlighted gray). Ala ...
Green Fluorescent Protein (GFP) Purification Kit
... removed from their agar plates and allowed to multiply in liquid nutrient media. The bacterial cells are then broken open (lysed) to release the Green Fluorescent Protein. GFP is subsequently purified from the contaminating bacterial debris using the disposable chromatography columns provided in thi ...
... removed from their agar plates and allowed to multiply in liquid nutrient media. The bacterial cells are then broken open (lysed) to release the Green Fluorescent Protein. GFP is subsequently purified from the contaminating bacterial debris using the disposable chromatography columns provided in thi ...
Protein 3D-structure analysis
... Find 3D-structures for transthyretin at RCSB PDB, then look for proteins with similar 3D-structure http://www.rcsb.org/pdb/explore/explore.do?structureId=1THC Similar structure: 2H6U What is the function of transthyretin, respectively of other proteins with similar structure? Have a look at the co ...
... Find 3D-structures for transthyretin at RCSB PDB, then look for proteins with similar 3D-structure http://www.rcsb.org/pdb/explore/explore.do?structureId=1THC Similar structure: 2H6U What is the function of transthyretin, respectively of other proteins with similar structure? Have a look at the co ...
Metal ion reconstitution studies of yeast copper
... [25]. The holo protein is first dialyzed against 100 mM acetate buffer at pH 3.8 in the presence of 10 mM EDTA (four times against 1 L each time). EDTA is then removed by dialysis against 100 mM acetate buffer (pH 3.8) in the presence of 100 mM NaCl (three times against 1 L each time). Metal-substit ...
... [25]. The holo protein is first dialyzed against 100 mM acetate buffer at pH 3.8 in the presence of 10 mM EDTA (four times against 1 L each time). EDTA is then removed by dialysis against 100 mM acetate buffer (pH 3.8) in the presence of 100 mM NaCl (three times against 1 L each time). Metal-substit ...
Chemistry 501 Lecture 3 Amino Acids
... • AA are optically active molecules and asymmetry of their mirror images is not superimposable (except in the case of glycine where the R-group is hydrogen) ...
... • AA are optically active molecules and asymmetry of their mirror images is not superimposable (except in the case of glycine where the R-group is hydrogen) ...
The trimethoprim-resistant dihydrofolate reductase associated with
... 10 fold concentrated TMENa-9 containing 6 M urea. Elution was with the same buffer mixture. Fractions of 5 ml were collected. The fractions containing dihydrofolate reductase were freed of urea by gel filtration in Sephadex G-2 5 (3.5x30 cm) equilibrated with 1O mM Na-phosphate, pH 6.8 containing 1 ...
... 10 fold concentrated TMENa-9 containing 6 M urea. Elution was with the same buffer mixture. Fractions of 5 ml were collected. The fractions containing dihydrofolate reductase were freed of urea by gel filtration in Sephadex G-2 5 (3.5x30 cm) equilibrated with 1O mM Na-phosphate, pH 6.8 containing 1 ...
Transport of amino acids (L-valine, L-lysine, L
... Lanfermeijer et al., 1991; McDonald et al., 1996). In addition, more recent work with faba bean and pea has shown that transcripts of a gene encoding a Hqusucrose symporter localize to the outer cell layers of the cotyledons (Harrington et al., 1997; Weber et al., 1997; Tegeder et al., 1999). Proton ...
... Lanfermeijer et al., 1991; McDonald et al., 1996). In addition, more recent work with faba bean and pea has shown that transcripts of a gene encoding a Hqusucrose symporter localize to the outer cell layers of the cotyledons (Harrington et al., 1997; Weber et al., 1997; Tegeder et al., 1999). Proton ...
www.iplantcollaborative.org
... D. Hedgecock et al. (2007) Transcriptomic analysis of growth heterosis in larval Pacific oysters (Crassostrea gigas) PNAS 104; p2313-2318 ...
... D. Hedgecock et al. (2007) Transcriptomic analysis of growth heterosis in larval Pacific oysters (Crassostrea gigas) PNAS 104; p2313-2318 ...
Highly Conserved Region 141–168 of the NS1 Protein Is a New
... The underlines indicate the restriction enzyme recognition sites. ...
... The underlines indicate the restriction enzyme recognition sites. ...
Leukaemia Section t(9;22)(q34;q11) in CML Atlas of Genetics and Cytogenetics
... course of the disease, they do not indicate the imminence of a blast crisis, although these additional anomalies also emerge frequently at the time of acute transformation; 2- These are: +der(22), +8, i(17q), +19, most often, but also: +21, -Y, -7, -17, +17; acute transformation can also be accompan ...
... course of the disease, they do not indicate the imminence of a blast crisis, although these additional anomalies also emerge frequently at the time of acute transformation; 2- These are: +der(22), +8, i(17q), +19, most often, but also: +21, -Y, -7, -17, +17; acute transformation can also be accompan ...
Protegrins: leukocyte antimicrobial peptides that combine features of
... 218TPlOlO column wtth a gradient (Buffer A, 0.1% TFA; Buffer B, 0.1% TFA in acetomtrtle) that increased in acetomtrtle concentratton The inset shows an AU-PAGE gel. stained with by l%.min-‘. Coomassie blue, that contams the starting mixture (M). composed of pooled Fractions 7678. and the indtvtdual ...
... 218TPlOlO column wtth a gradient (Buffer A, 0.1% TFA; Buffer B, 0.1% TFA in acetomtrtle) that increased in acetomtrtle concentratton The inset shows an AU-PAGE gel. stained with by l%.min-‘. Coomassie blue, that contams the starting mixture (M). composed of pooled Fractions 7678. and the indtvtdual ...
Development of Competitive Direct Enzyme
... serum and peroxidase-conjugated goat anti-mouse IgG. Each well of the microtiter plates was coated with 100 µl gentamicin-BSA conjugate (5 µg/ml) in 50 mM sodiumbicarbonate buffer (pH 9.6) and incubated for 3 h at 37°C. Unbound conjugate was removed from the plate using a washing solution (0.02% Twe ...
... serum and peroxidase-conjugated goat anti-mouse IgG. Each well of the microtiter plates was coated with 100 µl gentamicin-BSA conjugate (5 µg/ml) in 50 mM sodiumbicarbonate buffer (pH 9.6) and incubated for 3 h at 37°C. Unbound conjugate was removed from the plate using a washing solution (0.02% Twe ...
The Vps13p–Cdc31p complex is directly required for TGN late
... Kex2p) was from vps13Δ cells and acceptor MSS was from VPS13+ cells, a significant increase in PSHA cleavage was seen over the entire 20-min time course (Figs. 1 B and S1 A, red diamonds). These results indicate that direct, Gga-dependent TGN–PVC transport is completely defective when both donor and ...
... Kex2p) was from vps13Δ cells and acceptor MSS was from VPS13+ cells, a significant increase in PSHA cleavage was seen over the entire 20-min time course (Figs. 1 B and S1 A, red diamonds). These results indicate that direct, Gga-dependent TGN–PVC transport is completely defective when both donor and ...
ENZYME WEBQUEST Name
... Induced Fit 17. Observe the INDUCED FIT ANIMATION and describe what happens below: ...
... Induced Fit 17. Observe the INDUCED FIT ANIMATION and describe what happens below: ...
Type III secretion: The bacteria-eukaryotic cell
... the secreted proteins. Indeed, EM experiments provide evidence for the extrusion of the P. syringae effector proteins AvrPto and HrpZ from the tip of the Hrp pilus [6]. The proper assembly of the injectisome requires around 25 proteins, generally encoded by genes clustered in one locus. The precise f ...
... the secreted proteins. Indeed, EM experiments provide evidence for the extrusion of the P. syringae effector proteins AvrPto and HrpZ from the tip of the Hrp pilus [6]. The proper assembly of the injectisome requires around 25 proteins, generally encoded by genes clustered in one locus. The precise f ...
Glycolytic enzymes localize to ribonucleoprotein
... specification and contribute to the protection of germline DNA against transposons. These data provide the first evidence for the connection between metabolism and transposon silencing. The biochemical screen and mass spectrometry data reported here (Fig 1A) indicated that PyK is an abundant compone ...
... specification and contribute to the protection of germline DNA against transposons. These data provide the first evidence for the connection between metabolism and transposon silencing. The biochemical screen and mass spectrometry data reported here (Fig 1A) indicated that PyK is an abundant compone ...
She2p Is a Novel RNA Binding Protein
... 30 m3, Tyson et al. [1979]) suggests that She2p is present at a cellular concentration of about 230 nM. As She2p is localized in yeast cells, the effective concentration is almost certainly higher. Although this calculation is unlikely to provide an accurate estimate of She2p concentration in cells ...
... 30 m3, Tyson et al. [1979]) suggests that She2p is present at a cellular concentration of about 230 nM. As She2p is localized in yeast cells, the effective concentration is almost certainly higher. Although this calculation is unlikely to provide an accurate estimate of She2p concentration in cells ...
Disallowed Ramachandran Conformations of Amino Acid Residues
... Bulky hydrophobic residues which are found predominantly in well packed interiors of proteins like Leu, Ile, Val and Phe do not generally adopt disallowed conformations. The bulky charged residues Glu, Lys and Arg also have a relatively low propensity for backbone distortions. An unusually high prop ...
... Bulky hydrophobic residues which are found predominantly in well packed interiors of proteins like Leu, Ile, Val and Phe do not generally adopt disallowed conformations. The bulky charged residues Glu, Lys and Arg also have a relatively low propensity for backbone distortions. An unusually high prop ...
Novel NCC mutants and functional analysis in a new
... A total of seven identified NCC missense mutations, including five previously unidentified (Glu121Asp, Asn442Ser, Tyr489His, Pro751Leu and Gln1030Arg) and two known variants (Ser475Cys and Thr392Ile) were selected to study their effect on NCC function. Thr392Ile was picked, as it appeared de novo to ...
... A total of seven identified NCC missense mutations, including five previously unidentified (Glu121Asp, Asn442Ser, Tyr489His, Pro751Leu and Gln1030Arg) and two known variants (Ser475Cys and Thr392Ile) were selected to study their effect on NCC function. Thr392Ile was picked, as it appeared de novo to ...
Amino Acids, Proteins, and Enzymes
... In addition to the amino and carboxyl groups, amino acids have a side chain or R group attached to the α-carbon. Each amino acid has unique characteristics arising from the size, shape, solubility, and ionization properties of its R group. As a result, the side chains of amino acids exert a profound ...
... In addition to the amino and carboxyl groups, amino acids have a side chain or R group attached to the α-carbon. Each amino acid has unique characteristics arising from the size, shape, solubility, and ionization properties of its R group. As a result, the side chains of amino acids exert a profound ...
The ATP-Cone: An Evolutionarily Mobile, ATP
... metabolism in a ligand-dependent fashion. The presence of the conserved ATP-cone domain in ribonucleotide reductases of all three classes is of particular interest given the extreme divergence of the catalytic domain between class I+II and class III (Figure 3). In principle, this could indicate that ...
... metabolism in a ligand-dependent fashion. The presence of the conserved ATP-cone domain in ribonucleotide reductases of all three classes is of particular interest given the extreme divergence of the catalytic domain between class I+II and class III (Figure 3). In principle, this could indicate that ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.