Studying the Interaction Profiles of Nonnatural Amino Acids –
... large assemblies such as virus particles or muscle fibers. They also provide specific binding sites, as found in enzymes or proteins that carry oxygen and they regulate the function of DNA. The omnipresence of peptides and proteins in biological systems includes a wide variety of processes responsib ...
... large assemblies such as virus particles or muscle fibers. They also provide specific binding sites, as found in enzymes or proteins that carry oxygen and they regulate the function of DNA. The omnipresence of peptides and proteins in biological systems includes a wide variety of processes responsib ...
THE MULTIFARIOUS AND DYNAMIC REGULATION OF THE LIVING CELL Karen van Eunen
... In yeast it involves mechanisms in which the regulators interact directly with the ribosome or with associated initiation factors, thus influencing the recognition of the translation-initiation region by the ribosome complex [184]. In addition, regulatory factors may bind to untranslated regions (UT ...
... In yeast it involves mechanisms in which the regulators interact directly with the ribosome or with associated initiation factors, thus influencing the recognition of the translation-initiation region by the ribosome complex [184]. In addition, regulatory factors may bind to untranslated regions (UT ...
Data mining for important amino acid residues in multiple sequence
... secondary structures like the extremely regular local sub-structures α-helix and β-sheet which are linked by flexible regions, called loops. These folding patterns are mostly stabilized by backbone interactions like hydrogen bonds in which hydrogens are bound to highly electronegative atoms such as ...
... secondary structures like the extremely regular local sub-structures α-helix and β-sheet which are linked by flexible regions, called loops. These folding patterns are mostly stabilized by backbone interactions like hydrogen bonds in which hydrogens are bound to highly electronegative atoms such as ...
patrick_tb_ch18b
... Which of the following disadvantages apply to the above structure? Feedback: There are a variety of mechanisms by which tumour cells can gain resistance to drugs. For example, resistance to paclitaxel can arise due to mutation of tubulin resulting in a weakened binding interaction with paclitaxel. R ...
... Which of the following disadvantages apply to the above structure? Feedback: There are a variety of mechanisms by which tumour cells can gain resistance to drugs. For example, resistance to paclitaxel can arise due to mutation of tubulin resulting in a weakened binding interaction with paclitaxel. R ...
Wax ester fermentation and fatty acid biosynthesis in the facultatively
... et al. 2001; Butterfield 2000). At the time deep ocean water was still anoxic and the sulfide level was very high. The advent of oxygen took place during the time from 2.5 to ca 0.6 Ga (Canfield 1998; Anbar and Knoll 2001; Canfield et al. 2007) and the eukaryotes must have arisen and diversified by ...
... et al. 2001; Butterfield 2000). At the time deep ocean water was still anoxic and the sulfide level was very high. The advent of oxygen took place during the time from 2.5 to ca 0.6 Ga (Canfield 1998; Anbar and Knoll 2001; Canfield et al. 2007) and the eukaryotes must have arisen and diversified by ...
The semi-phosphorylative Entner–Doudoroff pathway in
... ment, the resulting crude extracts were diluted 1:1 with buffer A and subjected to a heat precipitation for 30 min at different temperatures. Extracts containing recombinant T. tenax and S. solfataricus protein were incubated at the following temperatures: T. tenax and S. solfataricus KD(P)G aldolas ...
... ment, the resulting crude extracts were diluted 1:1 with buffer A and subjected to a heat precipitation for 30 min at different temperatures. Extracts containing recombinant T. tenax and S. solfataricus protein were incubated at the following temperatures: T. tenax and S. solfataricus KD(P)G aldolas ...
Answers to Quiz Questions
... © 2008 Humana Press, a part of Springer Science+Business Media, LLC All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, 999 Riverview Drive, Suite 208, Totowa, NJ 07512 USA), except for brief excerpts in co ...
... © 2008 Humana Press, a part of Springer Science+Business Media, LLC All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, 999 Riverview Drive, Suite 208, Totowa, NJ 07512 USA), except for brief excerpts in co ...
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... domain (Riemer et al., 1993). An extensive biochemical and immunological search suggested the existence of only a single lamin protein in the mollusc Spisula soldissima (Dessev and Goldman, 1990). Also, the arthropod Drosophila melanogaster has long been thought to contain only a single nuclear lami ...
... domain (Riemer et al., 1993). An extensive biochemical and immunological search suggested the existence of only a single lamin protein in the mollusc Spisula soldissima (Dessev and Goldman, 1990). Also, the arthropod Drosophila melanogaster has long been thought to contain only a single nuclear lami ...
Mammalian CSAD and GADL1 have distinct biochemical properties
... data analysis” section below). The activated gel was transferred to an Immun-Blot nitrocellulose membrane (Bio-Rad) in 7 min using the Trans-Blot Turbo Transfer System (Bio-Rad) with Trans-Blot Turbo Midi Transfer Packs. Pre-made western blots of protein samples from mouse brain sampled at 11 differ ...
... data analysis” section below). The activated gel was transferred to an Immun-Blot nitrocellulose membrane (Bio-Rad) in 7 min using the Trans-Blot Turbo Transfer System (Bio-Rad) with Trans-Blot Turbo Midi Transfer Packs. Pre-made western blots of protein samples from mouse brain sampled at 11 differ ...
Candida rugosa - Universität Stuttgart
... Transesterification of (±)-menthol using propionic acid anhydride and Candida rugosa lipase was performed in chloroform and water at different pressures (1, 10, 50, and 100 bar) to study the pressure dependence of enantioselectivity E. As a result, E significantly decreased with increasing pressure ...
... Transesterification of (±)-menthol using propionic acid anhydride and Candida rugosa lipase was performed in chloroform and water at different pressures (1, 10, 50, and 100 bar) to study the pressure dependence of enantioselectivity E. As a result, E significantly decreased with increasing pressure ...
phosphoinositides: tiny lipids with giant impact on cell regulation
... the membranes and controlling vesicular trafficking and organelle physiology. The attention of scientists who study ion channels also turned toward PPIs as it became obvious that many channels or transporters require PPIs for their activity or control. The discovery of phosphatidylinositol 3-kinases ...
... the membranes and controlling vesicular trafficking and organelle physiology. The attention of scientists who study ion channels also turned toward PPIs as it became obvious that many channels or transporters require PPIs for their activity or control. The discovery of phosphatidylinositol 3-kinases ...
Characterization of the Sucrose Phosphate Phosphatase (SPP
... around 100 kDa, formed by subunits of approximately 50 kDa [19–21]. A carboxy-terminal domain of about 160 amino acids is present in higher plant SPPs that has been proposed to participate in dimerization (S6PPc domain), while prokaryotic forms of SPP are monomeric and lack this domain [2]. However, ...
... around 100 kDa, formed by subunits of approximately 50 kDa [19–21]. A carboxy-terminal domain of about 160 amino acids is present in higher plant SPPs that has been proposed to participate in dimerization (S6PPc domain), while prokaryotic forms of SPP are monomeric and lack this domain [2]. However, ...
Antimicrobial peptides in crustaceans
... its hydrophobic portion. The microorganisms are then destroyed via membrane destabilization and/or pore formation (Brogden, 2005; Yount et al., 2006). The detailed mechanism of pore formation (barrel-stave, toroidal and carpet-like models) has been described in detail elsewhere (Brogden, 2005; Saldi ...
... its hydrophobic portion. The microorganisms are then destroyed via membrane destabilization and/or pore formation (Brogden, 2005; Yount et al., 2006). The detailed mechanism of pore formation (barrel-stave, toroidal and carpet-like models) has been described in detail elsewhere (Brogden, 2005; Saldi ...
metabolism - Garland Science
... use many other minerals and elements, albeit in much smaller quantities. This means of nutrition, from inorganic compounds, is known as autotrophy (“self-feeding”). Organisms that obtain their carbon and nitrogen only from organic compounds—that is, ultimately, from plants—have a form of nutrition k ...
... use many other minerals and elements, albeit in much smaller quantities. This means of nutrition, from inorganic compounds, is known as autotrophy (“self-feeding”). Organisms that obtain their carbon and nitrogen only from organic compounds—that is, ultimately, from plants—have a form of nutrition k ...
Quercetin Attenuating Doxorubicin Induced Hepatic
... of alkaline copper reagent (Reagent C = 50mL of Reagent A + 0.5mL of Reagent B: Reagent A had 2% sodium carbonate and 0.1 N NaOH (w/v); Reagent B contained 0.5% copper sulphate in 1% sodium potassium tartarate). After adding reagent C, the reaction mixture was incubated at room temperature for 10 mi ...
... of alkaline copper reagent (Reagent C = 50mL of Reagent A + 0.5mL of Reagent B: Reagent A had 2% sodium carbonate and 0.1 N NaOH (w/v); Reagent B contained 0.5% copper sulphate in 1% sodium potassium tartarate). After adding reagent C, the reaction mixture was incubated at room temperature for 10 mi ...
NAD+-dependent formate dehydrogenase. From a model enzyme to
... (resolution 1.80 Å) [17], the ternary complex of enzyme with NAD+ and azide mimicking putative transition state (2.05 Å) [17], and a complex with ADPR (1.50 Å) [23]. Several other binary complexes of PseFDH (PseFDH-formate, PseFDH-NAD+, PseFDH-NADH) have been crystallized and preliminary X-ray data ...
... (resolution 1.80 Å) [17], the ternary complex of enzyme with NAD+ and azide mimicking putative transition state (2.05 Å) [17], and a complex with ADPR (1.50 Å) [23]. Several other binary complexes of PseFDH (PseFDH-formate, PseFDH-NAD+, PseFDH-NADH) have been crystallized and preliminary X-ray data ...
488KB - The Doudna Lab - University of California, Berkeley
... the platform and RNase IIIa domains (Fig. 1a). We were especially interested in this position of the protein because in the proposed model of dsRNA bound to G. intestinalis Dicer, the dsRNA had to be bent at the RNase IIIa–platform junction to allow a reasonable fit onto the Dicer crystal structure1 ...
... the platform and RNase IIIa domains (Fig. 1a). We were especially interested in this position of the protein because in the proposed model of dsRNA bound to G. intestinalis Dicer, the dsRNA had to be bent at the RNase IIIa–platform junction to allow a reasonable fit onto the Dicer crystal structure1 ...
Single-Amino Acid Substitutions Alter the Specificity and Affinity of
... products were subcloned into the EcoRI-XhoI sites of the pET-32a expression vector (Novagen, Inc., Madison, WI) which contains sequences encoding thioredoxin, an Nterminal 15-amino acid S tag, and a hexahistidine nickel binding motif. Clones with the correct sequence were electroporated into BL21(λD ...
... products were subcloned into the EcoRI-XhoI sites of the pET-32a expression vector (Novagen, Inc., Madison, WI) which contains sequences encoding thioredoxin, an Nterminal 15-amino acid S tag, and a hexahistidine nickel binding motif. Clones with the correct sequence were electroporated into BL21(λD ...
biochemical investigation into initiation of fatty acid synthesis in the
... parasite T. brucei. In my dissertation, I addressed various aspects of the regulation of TbACC, which catalyzes the first committed step in FA synthesis. In the second chapter, I hypothesized that TbACC is regulated in response to environmental lipids. I examined changes in TbACC RNA, protein abunda ...
... parasite T. brucei. In my dissertation, I addressed various aspects of the regulation of TbACC, which catalyzes the first committed step in FA synthesis. In the second chapter, I hypothesized that TbACC is regulated in response to environmental lipids. I examined changes in TbACC RNA, protein abunda ...
Enzyme Mechanisms - Weber State University
... • Thrombin is active only when converted from its inactive form, “prothrombin,” to thrombin by Factor X, another serine protease enzyme located in platelet membranes. • Prothrombin contains a number of glutamate residues that have been altered. • Following synthesis at the ribosome, the first 10 glu ...
... • Thrombin is active only when converted from its inactive form, “prothrombin,” to thrombin by Factor X, another serine protease enzyme located in platelet membranes. • Prothrombin contains a number of glutamate residues that have been altered. • Following synthesis at the ribosome, the first 10 glu ...
Aminolaevulinic acid synthase of Rhodobacter capsulatus: high
... the internal aldimine in a transaldimination reaction is common to both pathways [9,23]. The existence of the α-amino-β-oxoadipate intermediate has not been experimentally confirmed for ALAS catalysis as yet, but it is known that the corresponding intermediates occur in AONS reactions [21]. The enzy ...
... the internal aldimine in a transaldimination reaction is common to both pathways [9,23]. The existence of the α-amino-β-oxoadipate intermediate has not been experimentally confirmed for ALAS catalysis as yet, but it is known that the corresponding intermediates occur in AONS reactions [21]. The enzy ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.