Journal of Bacteriology
... considerable amount of activity was lost during incubation of the enzyme for 10 min at this temperature, probably because cofactors or substrates or both, which may protect the enzyme, were lacking. We were surprised to find that the specific activity of the L-alanine adding enzyme of E. coli strain ...
... considerable amount of activity was lost during incubation of the enzyme for 10 min at this temperature, probably because cofactors or substrates or both, which may protect the enzyme, were lacking. We were surprised to find that the specific activity of the L-alanine adding enzyme of E. coli strain ...
C/EBPα triggers proteasome‐dependent degradation of cdk4 during
... wild-type and C/EBPa-knockout animals indicated a complex regulation of cdk4 by C/EBPa. In addition to the direct inhibition of cdk4 activity, C/EBPa also controls protein levels of cdk4. Figure 1A shows western blotting analysis of cdk4 expression in nuclear extracts isolated from newborn livers. A ...
... wild-type and C/EBPa-knockout animals indicated a complex regulation of cdk4 by C/EBPa. In addition to the direct inhibition of cdk4 activity, C/EBPa also controls protein levels of cdk4. Figure 1A shows western blotting analysis of cdk4 expression in nuclear extracts isolated from newborn livers. A ...
Summary
... and secreted as a hexameric molecule in human HEK293 cells and purified to homogenity by lectin-affinity chromatography. Purified native hexameric BARF1 protein isolated from culture medium was used in ELISA. Considering that BARF1 mutation might affect immune recognition, the humoral immune respons ...
... and secreted as a hexameric molecule in human HEK293 cells and purified to homogenity by lectin-affinity chromatography. Purified native hexameric BARF1 protein isolated from culture medium was used in ELISA. Considering that BARF1 mutation might affect immune recognition, the humoral immune respons ...
Localization and nucleotide specificity of Blastocystis succinyl‐CoA
... Most of the research into the biochemistry and structure of eukaryotic SCS comes from animal model organisms, such as pig (Fraser et al., 2000; 2002). The limited information on protistan SCS suggests that eukaryotic SCS homologues are more diverse than generally presumed. For example, the Trichomon ...
... Most of the research into the biochemistry and structure of eukaryotic SCS comes from animal model organisms, such as pig (Fraser et al., 2000; 2002). The limited information on protistan SCS suggests that eukaryotic SCS homologues are more diverse than generally presumed. For example, the Trichomon ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... Cells contain limited pools of sequestered coenzyme A (CoA) that are essential for the activation of carboxylate metabolites. Esterification of carboxylic acids to CoA through the formation of a thioester bond is a common strategy used in metabolic processes to ‘activate’ the relevant metabolite. Th ...
... Cells contain limited pools of sequestered coenzyme A (CoA) that are essential for the activation of carboxylate metabolites. Esterification of carboxylic acids to CoA through the formation of a thioester bond is a common strategy used in metabolic processes to ‘activate’ the relevant metabolite. Th ...
Contents - Elsevier
... reduction systems, both based on nicotinamide but functionally independent. The experimentally measured ratio [NAD+] / [NADH] is much higher than the ratio [NADP+] / [NADPH]. Thus, these two coenzyme systems also can operate within a cell at different redox potentials. A related generalization that ...
... reduction systems, both based on nicotinamide but functionally independent. The experimentally measured ratio [NAD+] / [NADH] is much higher than the ratio [NADP+] / [NADPH]. Thus, these two coenzyme systems also can operate within a cell at different redox potentials. A related generalization that ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... but at the same time produced a higher background signal and non-specific bands (data not shown). Anti-rat CrAT antibodies are specific to rat CrAT and they do not recognise yeastexpressed rat COT (Fig. 3, lane 2), even though both enzymes share 36% amino acid sequence identity. The absence of bands ...
... but at the same time produced a higher background signal and non-specific bands (data not shown). Anti-rat CrAT antibodies are specific to rat CrAT and they do not recognise yeastexpressed rat COT (Fig. 3, lane 2), even though both enzymes share 36% amino acid sequence identity. The absence of bands ...
Saccharomyces cerevisiae Functional Expression and Stabilization of Horseradish Peroxidase by Directed Evolution in
... tested. Mutant libraries were screened for activity and their ability to survive incubation at 50°C in 0.5 mM H2O2 (see Materials and Methods). Libraries with a high mutation rate (>50% inactive clones) produced no mutants with increased activity or stability against H2O2 and elevated temperature (3 ...
... tested. Mutant libraries were screened for activity and their ability to survive incubation at 50°C in 0.5 mM H2O2 (see Materials and Methods). Libraries with a high mutation rate (>50% inactive clones) produced no mutants with increased activity or stability against H2O2 and elevated temperature (3 ...
Amino Acids in Animal Nutrition
... Amino acids found in proteins belong to the L-series. If an animal is supplied with amino acids in both the D- and L-forms (that is a 50:50 mixture of L- and D-amino acids, also called a “racemic mixture”) then the D-form has to be converted into the L-amino acid before it can participate in metabol ...
... Amino acids found in proteins belong to the L-series. If an animal is supplied with amino acids in both the D- and L-forms (that is a 50:50 mixture of L- and D-amino acids, also called a “racemic mixture”) then the D-form has to be converted into the L-amino acid before it can participate in metabol ...
Trichomonas vaginalis - Università degli Studi di Sassari
... The pathobiology of Trichomonas vaginalis involves direct and indirect interactions with host tissue, bacteria and viruses. In this work, we investigated adhesion and interaction with human microbiota, pathogenic mechanisms of protozoan. We characterized a new protein, TVAG339720, belonging to M60-l ...
... The pathobiology of Trichomonas vaginalis involves direct and indirect interactions with host tissue, bacteria and viruses. In this work, we investigated adhesion and interaction with human microbiota, pathogenic mechanisms of protozoan. We characterized a new protein, TVAG339720, belonging to M60-l ...
Chapter 6 Slides
... • Why are chaperones needed if the information for folding is inherent in the sequence? – to protect nascent proteins from the concentrated protein matrix in the cell and perhaps to accelerate slow steps ...
... • Why are chaperones needed if the information for folding is inherent in the sequence? – to protect nascent proteins from the concentrated protein matrix in the cell and perhaps to accelerate slow steps ...
PDF - School of Chemical Sciences
... close to the value of 1.0 ± 0.1 for this mutant with NADP (DV ⁄ KNADP). The slightly higher value for NAD (Table 2), may reflect that PTDH-E175A ⁄ A176R is not strictly ordered with respect to substrate binding because if NAD is the compulsory first substrate to bind, DV ⁄ K for the first substrate sho ...
... close to the value of 1.0 ± 0.1 for this mutant with NADP (DV ⁄ KNADP). The slightly higher value for NAD (Table 2), may reflect that PTDH-E175A ⁄ A176R is not strictly ordered with respect to substrate binding because if NAD is the compulsory first substrate to bind, DV ⁄ K for the first substrate sho ...
Structure of bacterial luciferase
... Structure of bacterial luciferaseBaldwin et the protease labile region that is nfissing from the [3 subunit. In the [~ subunit, the corresponding excursion consists o f - 3 5 residues. In the 0t subunit, amino acid residues from Phe272 to Thr288 were not seen in the electron density map [3°'], cons ...
... Structure of bacterial luciferaseBaldwin et the protease labile region that is nfissing from the [3 subunit. In the [~ subunit, the corresponding excursion consists o f - 3 5 residues. In the 0t subunit, amino acid residues from Phe272 to Thr288 were not seen in the electron density map [3°'], cons ...
please refer to anzfa`s guide to applications and proposals for a
... for food, feed and environmental approvals in Canada (Health Canada and the Canadian Food Inspection Agency). Regulatory submissions for food import approvals have also been made in Mexico and the European Union. The Applicant has advised that further submissions for import approvals in key internat ...
... for food, feed and environmental approvals in Canada (Health Canada and the Canadian Food Inspection Agency). Regulatory submissions for food import approvals have also been made in Mexico and the European Union. The Applicant has advised that further submissions for import approvals in key internat ...
Intracellular Localization of Interleukin-6 in
... IgG1) were used as irrelevant negative controls for these MoAbs (Becton Dickinson). A total of 2 µL of the supernatants were placed on nitrocellulose strips, allowed to dry, and blocked in 5% milk powder (Sigma). The blocked membrane strips were incubated with appropriate antibodies, and after exten ...
... IgG1) were used as irrelevant negative controls for these MoAbs (Becton Dickinson). A total of 2 µL of the supernatants were placed on nitrocellulose strips, allowed to dry, and blocked in 5% milk powder (Sigma). The blocked membrane strips were incubated with appropriate antibodies, and after exten ...
Flux analysis in central carbon metabolism in plants
... balances 3-fold. This resulted in a simplified system that offers the advantages of faster computation, and easier flux identifiability analysis. Furthermore, a computational technique called Boolean function mapping was introduced to aid in the formulation of bondomer or isotopomer balances. This t ...
... balances 3-fold. This resulted in a simplified system that offers the advantages of faster computation, and easier flux identifiability analysis. Furthermore, a computational technique called Boolean function mapping was introduced to aid in the formulation of bondomer or isotopomer balances. This t ...
Membrane transporters in a human genome-scale
... COBRA modeling are discussed elsewhere (Palsson, 2006;Thiele and Palsson, 2010a;Schellenberger et al., 2011). The comprehensive biochemical knowledge captured by GENREs includes gene-protein-reaction associations (GPRs), where individual metabolic/transport reactions are represented along with the g ...
... COBRA modeling are discussed elsewhere (Palsson, 2006;Thiele and Palsson, 2010a;Schellenberger et al., 2011). The comprehensive biochemical knowledge captured by GENREs includes gene-protein-reaction associations (GPRs), where individual metabolic/transport reactions are represented along with the g ...
Essentiality of Histidine in Ruminant and Other Animals Including
... ABSTRACT : Concept and establishment of essential amino acids in animals and human beings rendered immeasurable contributions to animal production and human health. In ruminant animals, however, essential amino acids have never been completely established. The present review proposes a hypothesis th ...
... ABSTRACT : Concept and establishment of essential amino acids in animals and human beings rendered immeasurable contributions to animal production and human health. In ruminant animals, however, essential amino acids have never been completely established. The present review proposes a hypothesis th ...
Purification and characterization of pyruvate decarboxylase from
... during the fermentation of glucose, but 100 mM-ethanol caused only a slight reduction (22%) in pyruvate decarboxylase activity. Phosphate increased the cooperativity and decreased the apparent affinity for pyruvate of pyruvate decarboxylase from Sacch. cerevisiae (Boiteux & Hess, 1970), but 100 mM-p ...
... during the fermentation of glucose, but 100 mM-ethanol caused only a slight reduction (22%) in pyruvate decarboxylase activity. Phosphate increased the cooperativity and decreased the apparent affinity for pyruvate of pyruvate decarboxylase from Sacch. cerevisiae (Boiteux & Hess, 1970), but 100 mM-p ...
Tag-ItTM Mutation Detection Kit for CFTR 70+6
... Conductance Regulator) protein which enhances the secretion of chloride in mucus producing epithelial cells. ...
... Conductance Regulator) protein which enhances the secretion of chloride in mucus producing epithelial cells. ...
Metabolic decision making by protein-metabolite - ETH E
... form of metabolites that modulate the activity of regulatory proteins. However, how this complex interplay of regulatory layers and metabolic feedback ultimately gives rise to a coordinated metabolic response is currently only poorly understood. The aim of this thesis is to elucidate ...
... form of metabolites that modulate the activity of regulatory proteins. However, how this complex interplay of regulatory layers and metabolic feedback ultimately gives rise to a coordinated metabolic response is currently only poorly understood. The aim of this thesis is to elucidate ...
ISOLATION OF PHYTASE PRODUCING BACTERIA FROM POULTRY FAECES AND
... inhibit a number of nutritionally important enzymes invivo [2]. It is to be noted that phytic acid, owing to its strong chelating ability forms cation-phytic acid complexes with multivalent cations [5]. In order to enhance the phosphate assimilation ability of simplestomached animal, phytases were a ...
... inhibit a number of nutritionally important enzymes invivo [2]. It is to be noted that phytic acid, owing to its strong chelating ability forms cation-phytic acid complexes with multivalent cations [5]. In order to enhance the phosphate assimilation ability of simplestomached animal, phytases were a ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.