Anti-Alpha Smooth Muscle Actin Antibody (Previously Covance
... Each lot of this antibody is quality control tested by immunohistochemical staining. The optimal working dilution should be determined for each specific assay condition. • WB: 1:100* • IHC: 1:50-1:100** Paraffin sections: This antibody can be used for labelling paraffin-embedded tissue sections fixe ...
... Each lot of this antibody is quality control tested by immunohistochemical staining. The optimal working dilution should be determined for each specific assay condition. • WB: 1:100* • IHC: 1:50-1:100** Paraffin sections: This antibody can be used for labelling paraffin-embedded tissue sections fixe ...
Anti-Alpha Smooth Muscle Actin Antibody (Previously
... Each lot of this antibody is quality control tested by immunohistochemical staining. The optimal working dilution should be determined for each specific assay condition. • WB: 1:100* • IHC: 1:50-1:100** Paraffin sections: This antibody can be used for labelling paraffin-embedded tissue sections fixe ...
... Each lot of this antibody is quality control tested by immunohistochemical staining. The optimal working dilution should be determined for each specific assay condition. • WB: 1:100* • IHC: 1:50-1:100** Paraffin sections: This antibody can be used for labelling paraffin-embedded tissue sections fixe ...
Chapter 3 The Plasma Membrane: transport across cell membrane
... Transport of a solute across a membrane down its concentration gradient or its electrochemical gradient, in which the cell expends no metabolic energy. 2.Ion channel Transmembrane protein complex that forms a water-filled channel across the membrane through which specific ions can diffuse down their ...
... Transport of a solute across a membrane down its concentration gradient or its electrochemical gradient, in which the cell expends no metabolic energy. 2.Ion channel Transmembrane protein complex that forms a water-filled channel across the membrane through which specific ions can diffuse down their ...
Proteins
... Stop codons have tRNA that recognize a signal to stop translation. Release factors bind to the ribosome which cause the peptidyl transferase to catalyze the addition of water to free the molecule and releases the polypeptide. ...
... Stop codons have tRNA that recognize a signal to stop translation. Release factors bind to the ribosome which cause the peptidyl transferase to catalyze the addition of water to free the molecule and releases the polypeptide. ...
Protein Structure - Computer Science, Stony Brook University
... Venclovas et al. Proteins, 2001 ...
... Venclovas et al. Proteins, 2001 ...
Analitical chemistry 1
... Agarose is a linear polysaccharide made up of the basic repeat unit agarobiose. Agarose is usually used at concentrations between 1% and 3%. ...
... Agarose is a linear polysaccharide made up of the basic repeat unit agarobiose. Agarose is usually used at concentrations between 1% and 3%. ...
Supplementary Material (doc 44K)
... in the lysate were determined by Bradford assays and confirmed by applying 10% of the lysate to SDS-PAGE stained with Coomassie blue. IP was carried out by incubating with anti-FLAG M2 (1:1000, Stratagene) antibody overnight and protein G beads for further 4 hours. After extensive washing (5 times w ...
... in the lysate were determined by Bradford assays and confirmed by applying 10% of the lysate to SDS-PAGE stained with Coomassie blue. IP was carried out by incubating with anti-FLAG M2 (1:1000, Stratagene) antibody overnight and protein G beads for further 4 hours. After extensive washing (5 times w ...
SSN Handouts
... • All three effector molecules above decrease the affinity of Hb for O2 . What will the curve look like? • P50 increases. You need to be at a higher pO2 to have 50% of the sites saturated. Hemoglobin in Disease: 1. Sickle cell anemia—point mutation in DNA coding for beta chain Autosomal recessive di ...
... • All three effector molecules above decrease the affinity of Hb for O2 . What will the curve look like? • P50 increases. You need to be at a higher pO2 to have 50% of the sites saturated. Hemoglobin in Disease: 1. Sickle cell anemia—point mutation in DNA coding for beta chain Autosomal recessive di ...
membranes (Ms. Shivani Bhagwat)
... category of transmembrane proteins. In humans, 27% of all proteins have been estimated to be alpha-helical membrane proteins. Beta-barrels. These proteins are so far found only in outer membranes of Gram-negative bacteria, cell wall of Gram-positive bacteria, and outer membranes of mitochondria and ...
... category of transmembrane proteins. In humans, 27% of all proteins have been estimated to be alpha-helical membrane proteins. Beta-barrels. These proteins are so far found only in outer membranes of Gram-negative bacteria, cell wall of Gram-positive bacteria, and outer membranes of mitochondria and ...
Effector Mechanisms of Immune Responses
... opsonization of an antigen • Explain the different mechanisms of complement ...
... opsonization of an antigen • Explain the different mechanisms of complement ...
antibody drug conjugates proprietary technology site specific / dar
... Increasing demand for targeted therapies, and more specifically for antibody drug conjugates in oncology, finds its scientific rationale and answers a high medical need : being able to deliver in a selective manner a cytotoxic drug into tumoral cells, with the promise to avoid healthy ones, by using ...
... Increasing demand for targeted therapies, and more specifically for antibody drug conjugates in oncology, finds its scientific rationale and answers a high medical need : being able to deliver in a selective manner a cytotoxic drug into tumoral cells, with the promise to avoid healthy ones, by using ...
Plasma Membrane - Motlow State Community College
... Diffusion of water across a selectively permeable membrane From high concentration of WATER to lower concentration of WATER Moves toward higher solute concentration ...
... Diffusion of water across a selectively permeable membrane From high concentration of WATER to lower concentration of WATER Moves toward higher solute concentration ...
Product Insert Sheet
... produce a mature soluble sequence. Epigen Recombinant Human ?produced in E.Coli is a single, non-glycosylated, ...
... produce a mature soluble sequence. Epigen Recombinant Human ?produced in E.Coli is a single, non-glycosylated, ...
200-Level Biochemistry
... Biotechnology is seen as a new industrial revolution, with the smoke stacks, factories and mines of the 19th Century being replaced with a knowledge-based industry of biochemical processes and information relating to genes, proteins and metabolism. In this course we will introduce you to the fundame ...
... Biotechnology is seen as a new industrial revolution, with the smoke stacks, factories and mines of the 19th Century being replaced with a knowledge-based industry of biochemical processes and information relating to genes, proteins and metabolism. In this course we will introduce you to the fundame ...
File
... Molecule Z will function at any temperature above 20°C. Molecule Z is composed of a string of molecular bases represented by A, T, G, and Molecule Z will function best at a specific pH. Molecule Z is not specific, so this reaction can be controlled by any other chemical in the body. ...
... Molecule Z will function at any temperature above 20°C. Molecule Z is composed of a string of molecular bases represented by A, T, G, and Molecule Z will function best at a specific pH. Molecule Z is not specific, so this reaction can be controlled by any other chemical in the body. ...
Unknown function, JCSG
... As part of its mission to increase structural coverage of protein families, JCSG is targeting proteins from the large CATH homologous superfamily 3.40.630.10 of zinc peptidases, which belong to the phosphorylase/hydrolase-like fold in SCOP and are comprised of proteins from several Pfam families (th ...
... As part of its mission to increase structural coverage of protein families, JCSG is targeting proteins from the large CATH homologous superfamily 3.40.630.10 of zinc peptidases, which belong to the phosphorylase/hydrolase-like fold in SCOP and are comprised of proteins from several Pfam families (th ...
Teacher`s Guide - Cornell Science Inquiry Partnerships
... explain the meaning of each program’s output. For example, SignalP and BLAST give results in terms of probability, so what do the numerical and graphical results mean? 4) To understand the biological relevance of bioinformatic analysis, and be confident in making inferences or defending conclusions ...
... explain the meaning of each program’s output. For example, SignalP and BLAST give results in terms of probability, so what do the numerical and graphical results mean? 4) To understand the biological relevance of bioinformatic analysis, and be confident in making inferences or defending conclusions ...
Proteins
... Most proteins contain all of the essential amino acids (EAAs)!! (except gelatin) Complete proteins supply all the EAAs needed to meet biologic requirements when consumed at recommended amounts ...
... Most proteins contain all of the essential amino acids (EAAs)!! (except gelatin) Complete proteins supply all the EAAs needed to meet biologic requirements when consumed at recommended amounts ...
Probabilistic Approaches to Predicting the Secondary Structure of Proteins
... the C=O group of residue n is hydrogen bonded to the N-H group of residue (n + 4). β-sheets have hydrogen bonds between C=O and N-H groups in distant regions of the same chain of amino acids, or even on different strands of residues. Consideration of the environment of the protein can also provide g ...
... the C=O group of residue n is hydrogen bonded to the N-H group of residue (n + 4). β-sheets have hydrogen bonds between C=O and N-H groups in distant regions of the same chain of amino acids, or even on different strands of residues. Consideration of the environment of the protein can also provide g ...
Modeling the Cell Membrane
... The job of the cell membrane is to both separate the cell from what surrounds it and, most importantly, control what is able to enter and exit the cell. The cell membrane is selectively permeable meaning that only some things are able to enter and leave the cell easily. Passive transport happens wit ...
... The job of the cell membrane is to both separate the cell from what surrounds it and, most importantly, control what is able to enter and exit the cell. The cell membrane is selectively permeable meaning that only some things are able to enter and leave the cell easily. Passive transport happens wit ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.