Acetylcholine Receptor-associated 43K Protein Contains Covalently
... Three 60-ram cultures of BC3Ht cells were labeled for 4 h with [3H]myristate and 43K protein was immunoprecipitated from the cell lysate with anti-43K serum. Immunoprecipitated proteins were resolved by SDS-PAGE and the region of the unfixed, undried gel containing 43K protein excised using prestain ...
... Three 60-ram cultures of BC3Ht cells were labeled for 4 h with [3H]myristate and 43K protein was immunoprecipitated from the cell lysate with anti-43K serum. Immunoprecipitated proteins were resolved by SDS-PAGE and the region of the unfixed, undried gel containing 43K protein excised using prestain ...
Basics of Fluorescence
... The word "tyrosine" is from the Greek tyros, meaning cheese, as it was first discovered in 1846 by German chemist Justus von Liebig in the protein casein from cheese. ...
... The word "tyrosine" is from the Greek tyros, meaning cheese, as it was first discovered in 1846 by German chemist Justus von Liebig in the protein casein from cheese. ...
Structural Biology: What does 3D tell us?
... Domains are the fundamental units of structure classification Different domains in a protein are also often associated with different functions carried out by the protein, though some functions occur at the interface between domains domain organization of P53 tumor suppressor ...
... Domains are the fundamental units of structure classification Different domains in a protein are also often associated with different functions carried out by the protein, though some functions occur at the interface between domains domain organization of P53 tumor suppressor ...
The Sec34/35 Golgi Transport Complex Is Related to the Exocyst
... McNew et al., 2000; Pelham, 2001). Although there are specific sets of SNARE proteins associated with each transport step, it is now clear that while they may contribute to the fidelity of fusion between membranes, other factors must also play a role. For several transport steps it has been possible ...
... McNew et al., 2000; Pelham, 2001). Although there are specific sets of SNARE proteins associated with each transport step, it is now clear that while they may contribute to the fidelity of fusion between membranes, other factors must also play a role. For several transport steps it has been possible ...
Lab 6
... When arabinose is present in the bacterium’s environment, arabinose binds with the AraC protein, forming a complex. This prevents the DNA loop from forming. The binding of arabinose also causes a change in the protein’s conformation (shape) resulting in the formation of a small pocket that will help ...
... When arabinose is present in the bacterium’s environment, arabinose binds with the AraC protein, forming a complex. This prevents the DNA loop from forming. The binding of arabinose also causes a change in the protein’s conformation (shape) resulting in the formation of a small pocket that will help ...
Metal Ion Transport and Storage
... • Charged Ions must pass through a Hydrophobic Membrane – Neutral gases (O2, CO2) and low charge density ions (anions) can move directly through the membrane – High charge density cations require help • Once inside the cell, metal ions must be transported to the location of their use, then released ...
... • Charged Ions must pass through a Hydrophobic Membrane – Neutral gases (O2, CO2) and low charge density ions (anions) can move directly through the membrane – High charge density cations require help • Once inside the cell, metal ions must be transported to the location of their use, then released ...
Silk-inspired polymers and proteins
... spiders (ADF-3 and ADF-4) that contain β-sheet-forming blocks of Ala6 or Ala8 and are known as eADF-3 and eADF-4 respectively [24]. Our proteins (molecular masses between 46 and 106 kDa) can be produced in high yield by high-density fermentation in E. coli (or other suitable hosts such as yeast) and ...
... spiders (ADF-3 and ADF-4) that contain β-sheet-forming blocks of Ala6 or Ala8 and are known as eADF-3 and eADF-4 respectively [24]. Our proteins (molecular masses between 46 and 106 kDa) can be produced in high yield by high-density fermentation in E. coli (or other suitable hosts such as yeast) and ...
Actin - WordPress.com
... o Monomers add to both ends, but add faster at the + end. o The Hydrolysis of ATP is slow. What happens when a low concentration of ATP is bound to G-Actin? o Treadmilling can occur if it is not too low. This is where monomers add to + end and come off of the – end at the same rate, causing the leng ...
... o Monomers add to both ends, but add faster at the + end. o The Hydrolysis of ATP is slow. What happens when a low concentration of ATP is bound to G-Actin? o Treadmilling can occur if it is not too low. This is where monomers add to + end and come off of the – end at the same rate, causing the leng ...
Subviral-Particle Biogenesis Hepatitis B Virus Small Surface
... days posttransfection, the culture supernatant was harvested and centrifuged for 10 min at 13,000 rpm, and 38 l of the supernatant was reduced and denatured with dithiothreitol and sodium dodecyl sulfate and used for polyacrylamide gel electrophoresis. Cells were washed once with 2 ml of phosphate- ...
... days posttransfection, the culture supernatant was harvested and centrifuged for 10 min at 13,000 rpm, and 38 l of the supernatant was reduced and denatured with dithiothreitol and sodium dodecyl sulfate and used for polyacrylamide gel electrophoresis. Cells were washed once with 2 ml of phosphate- ...
10 white P20 2 - Metabolomics Workbench
... N2 Dryer, Organomation Asscociates, Inc- OA-HEAT: Flip green power switch to “on” (located on bottom right of box). Of the three black switches, set the start/reset switch to neutral. Set the heat switch to neutral. Set the gas switch to Manual. To obtain gas flow, turn the gas nozzle on right side ...
... N2 Dryer, Organomation Asscociates, Inc- OA-HEAT: Flip green power switch to “on” (located on bottom right of box). Of the three black switches, set the start/reset switch to neutral. Set the heat switch to neutral. Set the gas switch to Manual. To obtain gas flow, turn the gas nozzle on right side ...
Catalysis by Enzymes
... Lectins are sugar-binding proteins which are highly specific for their sugar moieties. They typically play a role in biological recognition phenomena involving cells and proteins. For example, some bacteria use lectins to attach themselves to the cells of the host organism during infection. ...
... Lectins are sugar-binding proteins which are highly specific for their sugar moieties. They typically play a role in biological recognition phenomena involving cells and proteins. For example, some bacteria use lectins to attach themselves to the cells of the host organism during infection. ...
Using antibody catalysis to study the outcome of multiple
... and the light chains were expressed from two CMV promoters to give an IgG1 hybrid antibody with a human kappa constant region and human ␥1 constant region (C.R. and C.F.B., unpublished data). The antibodies were expressed in a human cell line and were secreted into the cell culture medium, from whic ...
... and the light chains were expressed from two CMV promoters to give an IgG1 hybrid antibody with a human kappa constant region and human ␥1 constant region (C.R. and C.F.B., unpublished data). The antibodies were expressed in a human cell line and were secreted into the cell culture medium, from whic ...
70-74 Research Article Molecular Docking Studies of Deacetylbisaco
... Docking technique is a method which predicts the preferred orientation of one molecule to a second when bound to each other to form a stable complex. Understanding the preferred orientation can be used to predict the strength of binding affinity between two molecules. As such, docking studies can be ...
... Docking technique is a method which predicts the preferred orientation of one molecule to a second when bound to each other to form a stable complex. Understanding the preferred orientation can be used to predict the strength of binding affinity between two molecules. As such, docking studies can be ...
Computational Biology
... Center for Protein Sequences) database (29). For this analysis, mitochondrial proteins were predicted with an accuracy of 70% as scored by the selfconsistency test. 10. Lecture WS 2003/04 ...
... Center for Protein Sequences) database (29). For this analysis, mitochondrial proteins were predicted with an accuracy of 70% as scored by the selfconsistency test. 10. Lecture WS 2003/04 ...
(1) Peter Mitchell and the Chemiosmotic Theory
... • In 1949, Morris Friedkin, together with his PhD supervisor, Albert Lehninger , showed the existence of a connection between different metabolic pathways for coenzyme NADH to oxygen as a source of energy in oxidative phosphorylation. ...
... • In 1949, Morris Friedkin, together with his PhD supervisor, Albert Lehninger , showed the existence of a connection between different metabolic pathways for coenzyme NADH to oxygen as a source of energy in oxidative phosphorylation. ...
Carbon Compounds 2-3 Foldable Instructions
... & lipids. It turns red in the presence of fats & lipids. Benedict’s solution is an indicator solution for simple sugars. It changes from blue to yellow, orange or red. Iodine solution is an indicator solution for complex sugars. It changes from brown to blue ...
... & lipids. It turns red in the presence of fats & lipids. Benedict’s solution is an indicator solution for simple sugars. It changes from blue to yellow, orange or red. Iodine solution is an indicator solution for complex sugars. It changes from brown to blue ...
C483 Exam I 2014 Answer Key
... ___T____The variable domains of antibodies are at the end of the heavy and light chain and interact with the antigen. ___F___ After a protein is denatured with denaturing agents, it cannot be renatured. ___F____ The tertiary structure of a protein may be formed from the folding of independent subuni ...
... ___T____The variable domains of antibodies are at the end of the heavy and light chain and interact with the antigen. ___F___ After a protein is denatured with denaturing agents, it cannot be renatured. ___F____ The tertiary structure of a protein may be formed from the folding of independent subuni ...
Targeting of interleukin-2 to the periplasm of
... that were located either in the middle of the cells or at the polar caps (Fig. 5). This confirmed previous findings by Devos et al. (1983) that IL-2 aggregates in the cytoplasm of E. coli. To overcome this, we have investigated the targeting of IL-2 to the periplasm of E. coli by fusing it either to ...
... that were located either in the middle of the cells or at the polar caps (Fig. 5). This confirmed previous findings by Devos et al. (1983) that IL-2 aggregates in the cytoplasm of E. coli. To overcome this, we have investigated the targeting of IL-2 to the periplasm of E. coli by fusing it either to ...
Slide 1
... A protein’s shape depends on four levels of structure Protein secondary structure results from coiling or folding of the polypeptide – Coiling results in a helical structure called an alpha helix – Folding may lead to a structure called a pleated sheet – Coiling and folding result from hydrogen b ...
... A protein’s shape depends on four levels of structure Protein secondary structure results from coiling or folding of the polypeptide – Coiling results in a helical structure called an alpha helix – Folding may lead to a structure called a pleated sheet – Coiling and folding result from hydrogen b ...
Test File
... 41. One turn of the cycle produces two reduced cofactors, _______ and _______, and the carbons are oxidized to two molecules of _______. 42. The reduced cofactors transfer their electrons to O2 to produce H2O via a set of four membrane-bound complexes collectively called the _______. These complexes ...
... 41. One turn of the cycle produces two reduced cofactors, _______ and _______, and the carbons are oxidized to two molecules of _______. 42. The reduced cofactors transfer their electrons to O2 to produce H2O via a set of four membrane-bound complexes collectively called the _______. These complexes ...
Organic Macromolecules
... What are the monomers for proteins Amino acids What is the relationship between an amino acid, polypeptide, and protein? Amino acids are linked together in a chain called a ...
... What are the monomers for proteins Amino acids What is the relationship between an amino acid, polypeptide, and protein? Amino acids are linked together in a chain called a ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... i. pectin. (5 marks) ii. classification of amino acids (15 marks) 30. a) i. Define active site. Explain the mechanism of enzyme action (8 marks). ii. Define enzyme inhibition. Explain the types of enzyme inhibition. (12 marks) ...
... i. pectin. (5 marks) ii. classification of amino acids (15 marks) 30. a) i. Define active site. Explain the mechanism of enzyme action (8 marks). ii. Define enzyme inhibition. Explain the types of enzyme inhibition. (12 marks) ...
Ammonium carbonate - Sigma
... incorporates ammonium carbonate has been published.2 Ammonium carbonate has been used in the isolation of cell surface proteins from Candida albicans.3 The use of ammonium carbonate in the comparative peptide analysis of equine, bovine, and canine cytochrome c by capillary electrophoresis/mass spect ...
... incorporates ammonium carbonate has been published.2 Ammonium carbonate has been used in the isolation of cell surface proteins from Candida albicans.3 The use of ammonium carbonate in the comparative peptide analysis of equine, bovine, and canine cytochrome c by capillary electrophoresis/mass spect ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.