Operon Models
... 7. Cut a piece of wire from a coat hanger and shove the wire into the repressor protein and bend the repressor protein into a shape so that it will not fit the operator region if the co-repressor (tryptophan) is not in place. 8. Write the word “tryptophan” on one of the tennis balls. Write “represso ...
... 7. Cut a piece of wire from a coat hanger and shove the wire into the repressor protein and bend the repressor protein into a shape so that it will not fit the operator region if the co-repressor (tryptophan) is not in place. 8. Write the word “tryptophan” on one of the tennis balls. Write “represso ...
Review Process - Molecular Systems Biology
... results provide a nice validation of the approach. As such, I think this manuscript is publishable in MSB. There are other papers out there looking at drug side effects (correctly cited), and this is the best one I've run across so far. I do have a few issues, however, which I would like to see addr ...
... results provide a nice validation of the approach. As such, I think this manuscript is publishable in MSB. There are other papers out there looking at drug side effects (correctly cited), and this is the best one I've run across so far. I do have a few issues, however, which I would like to see addr ...
prediction
... For a database containing N sequences with known tertiary (and hence secondary) structure, a prediction is made for one test sequence after training the method on the remaining training database containing the N1 remaining sequences (one-at-a-time jackknife testing). A complete jackknife test would ...
... For a database containing N sequences with known tertiary (and hence secondary) structure, a prediction is made for one test sequence after training the method on the remaining training database containing the N1 remaining sequences (one-at-a-time jackknife testing). A complete jackknife test would ...
INVESTIGATION INTO THE ALLOSTERIC REGULATION OF MITOTIC KINESIN EG5 Introduction Results
... yield a high-resolution structure of the Klp61F-L5 chimera motor domain-drug complex. Despite using the same crystallization techniques and conditions, there is a noticeable difference from the Klp61F-L5 crystals obtained in previous successful crystallization experiments (right). We believe this co ...
... yield a high-resolution structure of the Klp61F-L5 chimera motor domain-drug complex. Despite using the same crystallization techniques and conditions, there is a noticeable difference from the Klp61F-L5 crystals obtained in previous successful crystallization experiments (right). We believe this co ...
The Crystal Structure of Arabidopsis thaliana Allene Oxide Cyclase
... hydroperoxide cyclase involved in the conversion of 13(S)-HPOT into OPDA. The allene oxide 12,13-EOT was shown to serve as the immediate precursor of OPDA in plants (Baertschi et al., 1988; Crombie and Morgan, 1988; Hamberg and Hughes, 1988). Hamberg and Fahlstadius (1990) showed that this cyclizati ...
... hydroperoxide cyclase involved in the conversion of 13(S)-HPOT into OPDA. The allene oxide 12,13-EOT was shown to serve as the immediate precursor of OPDA in plants (Baertschi et al., 1988; Crombie and Morgan, 1988; Hamberg and Hughes, 1988). Hamberg and Fahlstadius (1990) showed that this cyclizati ...
Cereals, Pulses and Nuts - Cookery and Nutrition Beginners
... when grains are refined by a process called milling (for example, to produce white flour), the bran and germ are removed, leaving only the endosperm. This process causes a great loss of nutrients and fibre. Some fibre, vitamins and minerals may be added back (enriched) into refined cereal products ...
... when grains are refined by a process called milling (for example, to produce white flour), the bran and germ are removed, leaving only the endosperm. This process causes a great loss of nutrients and fibre. Some fibre, vitamins and minerals may be added back (enriched) into refined cereal products ...
Spinalin, a new glycine- and histidine
... 100 µg of the antigen per injection. For western blot analysis nematocyst proteins or total Hydra proteins were dissolved in SDS-sample buffer, separated by Tricine SDS-PAGE (Schägger and von Jagow, 1987), transferred to nitrocellulose membranes and incubated with spinalin19K antibody (1/1000 diluti ...
... 100 µg of the antigen per injection. For western blot analysis nematocyst proteins or total Hydra proteins were dissolved in SDS-sample buffer, separated by Tricine SDS-PAGE (Schägger and von Jagow, 1987), transferred to nitrocellulose membranes and incubated with spinalin19K antibody (1/1000 diluti ...
Slide 1
... Energy stored in NADH & FADH2 as electrons from the metabolic pathways is used for ATP synthesis by the process of oxidative phosphorylation When NADH and FADH2 are re-oxidized to NAD+ and FAD, the electrons released from them are transferred through a chain of electron carrier complexes (redox pro ...
... Energy stored in NADH & FADH2 as electrons from the metabolic pathways is used for ATP synthesis by the process of oxidative phosphorylation When NADH and FADH2 are re-oxidized to NAD+ and FAD, the electrons released from them are transferred through a chain of electron carrier complexes (redox pro ...
Rice 5 S Ribosomal RNA and Its Binding Protein Genes: Structure
... Amino acid sequence analysis of the RL5 protein revealed that it has many intriguing features. These include the presence of three repeated amino acid sequences and the conservation of glycine residues, which may he important for 5 S rRNA/RL5 protein interactions. Genomic DNA-blot analysis indicates ...
... Amino acid sequence analysis of the RL5 protein revealed that it has many intriguing features. These include the presence of three repeated amino acid sequences and the conservation of glycine residues, which may he important for 5 S rRNA/RL5 protein interactions. Genomic DNA-blot analysis indicates ...
Proteases: Hydrolysis of Peptide Bonds
... Caspase Cascade represents intracellular signaling pathways via proteolysis, with initiation, propagation, and termination phases. Initiation phase: Caspases activated hierarchically by protein-protein interactions to convert from single chain zymogens to two chain active proteases Propagation phase ...
... Caspase Cascade represents intracellular signaling pathways via proteolysis, with initiation, propagation, and termination phases. Initiation phase: Caspases activated hierarchically by protein-protein interactions to convert from single chain zymogens to two chain active proteases Propagation phase ...
Document
... The challenge is to turn this raw data into biological knowledge A valuable tool for this challenge is an automated diagnostic pipe through which newly determined sequences can be streamlined ...
... The challenge is to turn this raw data into biological knowledge A valuable tool for this challenge is an automated diagnostic pipe through which newly determined sequences can be streamlined ...
Docking of B-cell epitope antigen to specific hepatitis B antibody
... antigen and in understanding where important regions of an antigen are located in its three-dimensional structure. Therefore, mapping the interaction surface between antibody and antigen that bind each other is of outstanding interest in molecular immunology. Complementary to various experimental te ...
... antigen and in understanding where important regions of an antigen are located in its three-dimensional structure. Therefore, mapping the interaction surface between antibody and antigen that bind each other is of outstanding interest in molecular immunology. Complementary to various experimental te ...
Topology Prediction of Membrane Proteins
... All polar amide and carbonyl groups in the backbone are hydrogen bonded internally within the helix. This lowers the cost of transferring polar entities into the hydrocarbon interior and makes the conformation energetically stable. Another stabilizing factor is the enrichment of aromatic residues (T ...
... All polar amide and carbonyl groups in the backbone are hydrogen bonded internally within the helix. This lowers the cost of transferring polar entities into the hydrocarbon interior and makes the conformation energetically stable. Another stabilizing factor is the enrichment of aromatic residues (T ...
Problem Set 1 Solution
... f) Name the amino acid present in 1A3N that is substituted to Val6 in 2HBS. How does the nature of the side-chain of this amino acid differ from Val? Normal hemoglobin has glu6, which is substituted by val in 2HbS. The amino acid glu has a polar, hydrophilic side-chain in comparison to val6 which ha ...
... f) Name the amino acid present in 1A3N that is substituted to Val6 in 2HBS. How does the nature of the side-chain of this amino acid differ from Val? Normal hemoglobin has glu6, which is substituted by val in 2HbS. The amino acid glu has a polar, hydrophilic side-chain in comparison to val6 which ha ...
N-terminal signals
... •Let’s predict the secondary structure of the little transmembrane protein using a multiple sequence alignment with homologs. •Load littleMSA_fasta.txt on JalView •Calculate secondary structure prediction using Web Service > Secondary Structure Prediction > Jnet (Do not select any sequences when doi ...
... •Let’s predict the secondary structure of the little transmembrane protein using a multiple sequence alignment with homologs. •Load littleMSA_fasta.txt on JalView •Calculate secondary structure prediction using Web Service > Secondary Structure Prediction > Jnet (Do not select any sequences when doi ...
as PDF
... matrix synthesis and apoptosis. They play major roles during prenatal development and postnatal growth, remodeling, and maintenance of a variety of tissues and organs. In accordance with their role in regulating vital biological processes, the amino-acid sequences of homologous TGF-β proteins from d ...
... matrix synthesis and apoptosis. They play major roles during prenatal development and postnatal growth, remodeling, and maintenance of a variety of tissues and organs. In accordance with their role in regulating vital biological processes, the amino-acid sequences of homologous TGF-β proteins from d ...
Thermal unfolding of proteins at high pH range studied by UV
... The UV absorbance changes following tyrosyl ionization can be used to monitor protein unfolding. Buried tyrosine residues are not affected by the pH. The unfolding of the protein molecule leads to the exposure of these residues at the protein surface and therefore tyrosyl ionization is detected. In ...
... The UV absorbance changes following tyrosyl ionization can be used to monitor protein unfolding. Buried tyrosine residues are not affected by the pH. The unfolding of the protein molecule leads to the exposure of these residues at the protein surface and therefore tyrosyl ionization is detected. In ...
Reprogramming Cells to Fight Disease
... develop drugs one at a time? Individual proteins are completely different molecules from each other, and therefore the production and delivery of each is highly specific to that protein. The mRNAs that code for these proteins, however, are remarkably similar to each other, meaning that once the proc ...
... develop drugs one at a time? Individual proteins are completely different molecules from each other, and therefore the production and delivery of each is highly specific to that protein. The mRNAs that code for these proteins, however, are remarkably similar to each other, meaning that once the proc ...
The Lamin B Receptor of the Nuclear Envelope Inner Membrane: A
... integral membrane proteins that may be involved in the anchorage of these structures. In support of this notion, several integral membrane proteins associated with these structures have been identified and localized to specific nuclear envelope membrane domains. An integral membrane glycoprotein (gp ...
... integral membrane proteins that may be involved in the anchorage of these structures. In support of this notion, several integral membrane proteins associated with these structures have been identified and localized to specific nuclear envelope membrane domains. An integral membrane glycoprotein (gp ...
Homology Claims
... If the specification provides sufficient support that the isolated protein has a specific, substantial, and credible use related to activity X, limiting the claims to proteins having activity X may help resolve a scope of enablement issue. ...
... If the specification provides sufficient support that the isolated protein has a specific, substantial, and credible use related to activity X, limiting the claims to proteins having activity X may help resolve a scope of enablement issue. ...
PowerPoint
... Genetic code scoring system – This assumes that changes in protein sequence arise from mutations. If only one point mutation is needed to change a given AA to another (at a specific position in alignment), the two amino-acids are more closely related than if two point mutations were required. Physic ...
... Genetic code scoring system – This assumes that changes in protein sequence arise from mutations. If only one point mutation is needed to change a given AA to another (at a specific position in alignment), the two amino-acids are more closely related than if two point mutations were required. Physic ...
Chapter 7
... • Facilitated diffusion is still passive because the solute moves down its concentration gradient • Some transport proteins, however, can move solutes against their concentration gradients ...
... • Facilitated diffusion is still passive because the solute moves down its concentration gradient • Some transport proteins, however, can move solutes against their concentration gradients ...
Purified dextransucrase from Pediococcus pentosaceus CRAG3 as
... determination—The dextransucrase assay was carried out in 1 mL reaction mixture containing 5% (w/v) sucrose in 20 mM sodium acetate buffer (pH 5.4) and 20 µL of enzyme sample. The enzyme reaction was performed at 30 oC for 15 min. An aliquot (100 µL) was taken from the reaction mixture and the reduc ...
... determination—The dextransucrase assay was carried out in 1 mL reaction mixture containing 5% (w/v) sucrose in 20 mM sodium acetate buffer (pH 5.4) and 20 µL of enzyme sample. The enzyme reaction was performed at 30 oC for 15 min. An aliquot (100 µL) was taken from the reaction mixture and the reduc ...
SeeProtStructFromPDB
... The second frame contains a text box for entering commands, and a message box that displays information about the structure or a particular part of the structure that has been selected. The third frame, containing the actual structure, is the largest. Right clicking on the structure (Windows users) ...
... The second frame contains a text box for entering commands, and a message box that displays information about the structure or a particular part of the structure that has been selected. The third frame, containing the actual structure, is the largest. Right clicking on the structure (Windows users) ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.