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... We report the solution of the crystal structure of a mutant of the immunoglobulin VL domain of the antibody McPC603, in which the complementarity-determining region 1 segment is replaced with that of a different antibody . The wild-type and mutant crystal structures have been refined to a crystallog ...
... We report the solution of the crystal structure of a mutant of the immunoglobulin VL domain of the antibody McPC603, in which the complementarity-determining region 1 segment is replaced with that of a different antibody . The wild-type and mutant crystal structures have been refined to a crystallog ...
Structure and physical-chemical properties of enzymes
... Maple syrup urine disease - the disorder of the oxidative decarboxylation of -ketoacids derived from valine, isoleucine, and leucine caused by the missing or defect of branched-chain dehydrogenase. The levels of branched-chain amino acids and corresponding -ketoacids are markedly elevated in both ...
... Maple syrup urine disease - the disorder of the oxidative decarboxylation of -ketoacids derived from valine, isoleucine, and leucine caused by the missing or defect of branched-chain dehydrogenase. The levels of branched-chain amino acids and corresponding -ketoacids are markedly elevated in both ...
PPT
... Structural genomics consists in the determination of the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography, NMR spectroscopy or computational approaches such as homology modelling. As opposed to traditional structural biology, the ...
... Structural genomics consists in the determination of the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography, NMR spectroscopy or computational approaches such as homology modelling. As opposed to traditional structural biology, the ...
PicodropTM
... contamination or carry-over on the sample platform. The advanced polymer technology used to make the UVpette tips ensures precise UV absorbtion measurements between 220nm and 950nm, making it ideal for applications involving nucleic acid, protein or microarray dye concentration determination. ...
... contamination or carry-over on the sample platform. The advanced polymer technology used to make the UVpette tips ensures precise UV absorbtion measurements between 220nm and 950nm, making it ideal for applications involving nucleic acid, protein or microarray dye concentration determination. ...
Protein synthesis: Twenty three amino acids and
... ensured that they minimized the sequence space that had to be sampled at each step of their selection procedure. Another key point in this approach was the use of tyrosyltRNA synthetase, which does not have any known editing activity. The principles of this approach are not specific to a particular ...
... ensured that they minimized the sequence space that had to be sampled at each step of their selection procedure. Another key point in this approach was the use of tyrosyltRNA synthetase, which does not have any known editing activity. The principles of this approach are not specific to a particular ...
Quiz - Columbus Labs
... catalyzes GDP-GTP exchange on the α -subunit of the heterotrimer G protein (Gαβγ ), replacing GDP with GTP. The Gα -subunit with GTP bound dissociates from the βγ -subunits and binds to adenylyl cyclase (AC). AC becomes active upon association with Gα :GTP and catalyzes the formation of cAMP from AT ...
... catalyzes GDP-GTP exchange on the α -subunit of the heterotrimer G protein (Gαβγ ), replacing GDP with GTP. The Gα -subunit with GTP bound dissociates from the βγ -subunits and binds to adenylyl cyclase (AC). AC becomes active upon association with Gα :GTP and catalyzes the formation of cAMP from AT ...
GrpL, a Grb2-related Adaptor Protein, Interacts with SLP-76 to
... finally a COOH-terminal SH3 domain (Fig. 1 A). A comparison to other known proteins available in current sequence data banks revealed that this protein is most similar to the adaptor proteins, Grb2 and Grap (Fig. 1 B and Table I). Based on this similarity, we have designated this molecule, GrpL for ...
... finally a COOH-terminal SH3 domain (Fig. 1 A). A comparison to other known proteins available in current sequence data banks revealed that this protein is most similar to the adaptor proteins, Grb2 and Grap (Fig. 1 B and Table I). Based on this similarity, we have designated this molecule, GrpL for ...
Claudins, dietary milk proteins, and intestinal barrier regulation
... tion of the Smad-4 binding site of the claudin-4 promoter, established that Smad-4 binding was required for the ability of both TGFb1 and WPC1 to activate the promoter. Thus, HT-29/B6 cells treated with TGFb1 (60 ng/L) for 48 hours showed an increase in the activity of the claudin-4 promoter. Overe ...
... tion of the Smad-4 binding site of the claudin-4 promoter, established that Smad-4 binding was required for the ability of both TGFb1 and WPC1 to activate the promoter. Thus, HT-29/B6 cells treated with TGFb1 (60 ng/L) for 48 hours showed an increase in the activity of the claudin-4 promoter. Overe ...
Sequencing the World of Possibilities for Energy & Environment
... Sequencing the World of Possibilities for Energy & Environment ...
... Sequencing the World of Possibilities for Energy & Environment ...
Trends in Sports Drink Formulations
... saturation of the glucose transport mechanism. Once this metabolic pathway becomes saturated, the body will not absorb any more glucose for the time being. However, this study from U.K. researchers found that combining more than one sugar (in this case glucose and fructose) allowed for greater carbo ...
... saturation of the glucose transport mechanism. Once this metabolic pathway becomes saturated, the body will not absorb any more glucose for the time being. However, this study from U.K. researchers found that combining more than one sugar (in this case glucose and fructose) allowed for greater carbo ...
Nanosep® Centrifugal Devices - Protocols for Use
... Synthesis of radioactively-labeled DNA is one of the most commonly used procedures in molecular biology labs. These sequence-specific labeled DNA fragments can be hybridized to specific DNA sequences bound to membranes, allowing them to be detected by autoradiography. Recently, a variety of non-radi ...
... Synthesis of radioactively-labeled DNA is one of the most commonly used procedures in molecular biology labs. These sequence-specific labeled DNA fragments can be hybridized to specific DNA sequences bound to membranes, allowing them to be detected by autoradiography. Recently, a variety of non-radi ...
Biochemistry of Sulfur
... a leader peptide with a twin-arginine translocation motif (TAT) not present in the mature protein suggesting a transport across the membrane (Fig. 7). Fe and Ni were present in membrane and in enriched hydrogenase fractions in accordance with the observed sequence similarity. The Ac. ambivalens SR g ...
... a leader peptide with a twin-arginine translocation motif (TAT) not present in the mature protein suggesting a transport across the membrane (Fig. 7). Fe and Ni were present in membrane and in enriched hydrogenase fractions in accordance with the observed sequence similarity. The Ac. ambivalens SR g ...
An Efficient Protocol for Identifying Separation-of-Function
... ABSTRACT Mutations that confer the loss of a single biochemical property (separation-of-function mutations) can often uncover a previously unknown role for a protein in a particular biological process. However, most mutations are identified based on loss-offunction phenotypes, which cannot differenti ...
... ABSTRACT Mutations that confer the loss of a single biochemical property (separation-of-function mutations) can often uncover a previously unknown role for a protein in a particular biological process. However, most mutations are identified based on loss-offunction phenotypes, which cannot differenti ...
Document
... • example: hydration of a cis alkene (but not its trans isomer) to give an R alcohol (but not the S alcohol) • Review Cahn–Ingold–Prelog priority rules (R/S) at Wikipedia. ...
... • example: hydration of a cis alkene (but not its trans isomer) to give an R alcohol (but not the S alcohol) • Review Cahn–Ingold–Prelog priority rules (R/S) at Wikipedia. ...
Expt. 2 Bioinformatics
... Computers, the Web and Bioinformatics The computer is a critical tool in laboratory research, particularly in biochemistry. All major pieces of scientific equipment in research laboratories are connected to a computer to enable data collection, particularly over extended periods of time. Not only is ...
... Computers, the Web and Bioinformatics The computer is a critical tool in laboratory research, particularly in biochemistry. All major pieces of scientific equipment in research laboratories are connected to a computer to enable data collection, particularly over extended periods of time. Not only is ...
WAVE/Scars in platelets
... The Triton X-100–insoluble cytoskeleton was isolated as previously described.27 An equal amount of lysis buffer was added to the platelet suspensions to solubilize the platelets. After 5 minutes on ice, the lysate was centrifuged at 10 000g. The resulting pellet was washed twice in washing buffer. F ...
... The Triton X-100–insoluble cytoskeleton was isolated as previously described.27 An equal amount of lysis buffer was added to the platelet suspensions to solubilize the platelets. After 5 minutes on ice, the lysate was centrifuged at 10 000g. The resulting pellet was washed twice in washing buffer. F ...
Chemistry 110 Enzymes
... product of a series of enzyme-catalyzed reactions inhibits an earlier reaction in a sequence. The inhibition may be competitive or noncompetitive. ¾A proenzyme or zymogen is an inactive form of an enzyme that must have part of its polypeptide chain cleaved before it becomes active. An example is try ...
... product of a series of enzyme-catalyzed reactions inhibits an earlier reaction in a sequence. The inhibition may be competitive or noncompetitive. ¾A proenzyme or zymogen is an inactive form of an enzyme that must have part of its polypeptide chain cleaved before it becomes active. An example is try ...
Unknown title - Sigma
... applications of such strategies for example relate to larger membrane proteins14,15. In addition, block labeling16,17 as well as reverse18 labeling strategies have successfully been used in ssNMR. In these experiments, a dedicated set of aminoacid precursors or amino acids is used during expression. ...
... applications of such strategies for example relate to larger membrane proteins14,15. In addition, block labeling16,17 as well as reverse18 labeling strategies have successfully been used in ssNMR. In these experiments, a dedicated set of aminoacid precursors or amino acids is used during expression. ...
Middle-Term Test Paper on Biochemistry
... Isoenzymes ( isozymes ) are different forms of an enzyme which catalyze the same reaction, but exhibit different physical or kinetic properties, such as isoelectric point, pH optimum, substrate affinity or effect of inhibitors. 3) zymogen and its activation Several enzymes are synthesized as larger ...
... Isoenzymes ( isozymes ) are different forms of an enzyme which catalyze the same reaction, but exhibit different physical or kinetic properties, such as isoelectric point, pH optimum, substrate affinity or effect of inhibitors. 3) zymogen and its activation Several enzymes are synthesized as larger ...
Spermatogenesis-preventing substance - Development
... a boiling water bath for 5 minutes, centrifuged at 9000 g for 5 minutes, and then the supernatant of the tissue extracts was collected. Samples were separated using SDS-PAGE on 10% gels. The separated proteins were transferred to polyvinylidene difluoride membranes (Millipore). After blotting, the m ...
... a boiling water bath for 5 minutes, centrifuged at 9000 g for 5 minutes, and then the supernatant of the tissue extracts was collected. Samples were separated using SDS-PAGE on 10% gels. The separated proteins were transferred to polyvinylidene difluoride membranes (Millipore). After blotting, the m ...
full size
... control is an enzyme-regulation process where the product of a series of enzyme-catalyzed reactions inhibits an earlier reaction in a sequence. The inhibition may be competitive or noncompetitive. ¾A proenzyme or zymogen is an inactive form of an enzyme that must have part of its polypeptide chain c ...
... control is an enzyme-regulation process where the product of a series of enzyme-catalyzed reactions inhibits an earlier reaction in a sequence. The inhibition may be competitive or noncompetitive. ¾A proenzyme or zymogen is an inactive form of an enzyme that must have part of its polypeptide chain c ...
Computational Tools For Protein Modeling
... of structure and function. A correlated mutation between two residue positions can be used to predict a probable physical contact in structure [15] using programs such as WHATIF [16]. A profile derived from multiple sequence alignment is often more sensitive with less noise than the information prov ...
... of structure and function. A correlated mutation between two residue positions can be used to predict a probable physical contact in structure [15] using programs such as WHATIF [16]. A profile derived from multiple sequence alignment is often more sensitive with less noise than the information prov ...
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis
... subsequently used for visualizing the stained nucleic acids. Throughout this chapter, the common methods for staining and visualization of DNA are described in details. Agarose gel electrophoresis provides multiple advantages that make it widely popular. For example, nucleic acids are not chemically ...
... subsequently used for visualizing the stained nucleic acids. Throughout this chapter, the common methods for staining and visualization of DNA are described in details. Agarose gel electrophoresis provides multiple advantages that make it widely popular. For example, nucleic acids are not chemically ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.