Unraveling the mechanisms of RNA
... affinity of the U1A/U1hpII interaction by more than 100fold. Measurements of the individual rate constants further showed that this loss of affinity was over-whelmingly due to a decrease in association rate. Although as expected, increasing salt concentration also reduced the association rates for the ...
... affinity of the U1A/U1hpII interaction by more than 100fold. Measurements of the individual rate constants further showed that this loss of affinity was over-whelmingly due to a decrease in association rate. Although as expected, increasing salt concentration also reduced the association rates for the ...
Hemoglobin Receptor in Leishmania Is a Hexokinase Located in the
... fractions were collected. The protein profile of each fraction was determined by SDS-PAGE followed by silver staining. HbR-containing fractions were pooled and dialyzed against LSB. Detection of Hb Binding with HbR—Determination of Hb binding with HbR was carried out using biotinylated hemoglobin (B ...
... fractions were collected. The protein profile of each fraction was determined by SDS-PAGE followed by silver staining. HbR-containing fractions were pooled and dialyzed against LSB. Detection of Hb Binding with HbR—Determination of Hb binding with HbR was carried out using biotinylated hemoglobin (B ...
Heinrichs, V., and Baker, B. S.
... region, and the SR domain to activate the female-specific dsx splicing pattern in tissue culture. For this purpose, RBP1 expression constructs were cotransfected together with a dsx minigene construct into Drosophila Schneider (SL2) cells as described recently (15). Overexpression of several RBP1 pr ...
... region, and the SR domain to activate the female-specific dsx splicing pattern in tissue culture. For this purpose, RBP1 expression constructs were cotransfected together with a dsx minigene construct into Drosophila Schneider (SL2) cells as described recently (15). Overexpression of several RBP1 pr ...
prosthetic group as non polypeptide biocatalyst essential for
... furniture into your house and up a few flights of stairs well, friends are good to have in situations like this, particularly strong ones who will lift anything for some ...
... furniture into your house and up a few flights of stairs well, friends are good to have in situations like this, particularly strong ones who will lift anything for some ...
Defining How Botulinum Toxin Binds to the
... helical conformation on binding to a hydrophobic groove within the binding domain of BoNT/B. This is further validated by mutagenesis of residues on Syt-II in this region, carried out as part of our studies, which is observed to negatively affect BoNT/B binding. In addition, our molecular docking st ...
... helical conformation on binding to a hydrophobic groove within the binding domain of BoNT/B. This is further validated by mutagenesis of residues on Syt-II in this region, carried out as part of our studies, which is observed to negatively affect BoNT/B binding. In addition, our molecular docking st ...
BPM§1.2_Protein Struktur.key
... This describes the overall shape of the domain structure as determined by the orientations of the secondary structures but ignores the connectivity between the secondary structures. ...
... This describes the overall shape of the domain structure as determined by the orientations of the secondary structures but ignores the connectivity between the secondary structures. ...
Onset of lactation in the bovine mammary gland:
... USA). The membrane was rinsed three times in TBS and incubated for 1 h at room temperature in TBS containing 1% (wt/vol) non-fat milk powder and 1:2,000 dilution of a horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (Invitrogen). The membrane was finally washed five times in ...
... USA). The membrane was rinsed three times in TBS and incubated for 1 h at room temperature in TBS containing 1% (wt/vol) non-fat milk powder and 1:2,000 dilution of a horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (Invitrogen). The membrane was finally washed five times in ...
Chen-6-Translation
... acids to the C-terminus of the growing polypeptide • Synthesis of each peptide bond requires energy derived from the cleavage of the AA-tRNA ester bond. The ribosomal enzyme doing this is called Peptidyl Transferase • Elongation is repeated as many times as there are codons in the mRNA ...
... acids to the C-terminus of the growing polypeptide • Synthesis of each peptide bond requires energy derived from the cleavage of the AA-tRNA ester bond. The ribosomal enzyme doing this is called Peptidyl Transferase • Elongation is repeated as many times as there are codons in the mRNA ...
3.6: ENZYMES - HS Biology IB
... random collisions more frequent; activity levels off / plateau; high concentration no change in activity; as all active sites fully utilised; [8 max] Award [6 max] if only specificity or substrate concentration aspects addressed. ...
... random collisions more frequent; activity levels off / plateau; high concentration no change in activity; as all active sites fully utilised; [8 max] Award [6 max] if only specificity or substrate concentration aspects addressed. ...
Influence of Valine Analogues on Protein Synthesis of Chicken
... Statistical analysis of data was performed by one-way ANOVA using the General Linear Model Procedures of SAS (SAS/STAT version 6) (SAS Institute, 1999). After ANOVA, a multiple comparison procedure (Dunnett’s test) was performed to compare each treatment and control. Differences between means were c ...
... Statistical analysis of data was performed by one-way ANOVA using the General Linear Model Procedures of SAS (SAS/STAT version 6) (SAS Institute, 1999). After ANOVA, a multiple comparison procedure (Dunnett’s test) was performed to compare each treatment and control. Differences between means were c ...
Second bioinformatics lab:Exercise on disease
... gives the highest score for identical residues. Substitutions are scored based on how conservative the changes are (a nonpolar small amino acid replaced by a nonpolar large amino acid). The output shows a list of sequences, with the highest scoring sequence at the top. The scoring output is given as ...
... gives the highest score for identical residues. Substitutions are scored based on how conservative the changes are (a nonpolar small amino acid replaced by a nonpolar large amino acid). The output shows a list of sequences, with the highest scoring sequence at the top. The scoring output is given as ...
Chapter 7 7 The Behavior of Proteins: Enzymes Mechanisms and
... also so se serves es as a positive (stimulatory) modulator, or activator; e.g., the binding g of aspartate p ((its substrate)) to ATCase. ((tends to be positive regulator) • hetero heterotropic tropic p effects: allosteric interactions that occur when different substances are bound to the protein; e ...
... also so se serves es as a positive (stimulatory) modulator, or activator; e.g., the binding g of aspartate p ((its substrate)) to ATCase. ((tends to be positive regulator) • hetero heterotropic tropic p effects: allosteric interactions that occur when different substances are bound to the protein; e ...
Amassin, an olfactomedin protein, mediates the massive
... The clotting of coelomocytes can be demonstrated by pouring coelomic fluid into a beaker, swirling it gently, and observing with the unaided eye. Clot formation is visible within 50 s and is complete by 150 s (Fig. 1). Clots settle onto the substratum; the cells spread out at the edges of the clots ...
... The clotting of coelomocytes can be demonstrated by pouring coelomic fluid into a beaker, swirling it gently, and observing with the unaided eye. Clot formation is visible within 50 s and is complete by 150 s (Fig. 1). Clots settle onto the substratum; the cells spread out at the edges of the clots ...
BMC Genomics Expansion of the Bactericidal/Permeability Increasing-like (BPI-like) protein locus in cattle
... The remaining five are most similar to PSP from mouse and human, for which there is no unambiguous ortholog in bovine (see Table 2 for % amino acid identities). Two of these have been previously described as BSP30A and BSP30B [19]. We therefore named the two additional ...
... The remaining five are most similar to PSP from mouse and human, for which there is no unambiguous ortholog in bovine (see Table 2 for % amino acid identities). Two of these have been previously described as BSP30A and BSP30B [19]. We therefore named the two additional ...
Identification of proteins that putatively bind the
... I represent and warrant this is my original work, and does not infringe or violate any rights of others. I warrant that I have obtained written permissions from the owner of any third party copyrighted material included in these files. I acknowledge that I retain ownership rights to the copyright of ...
... I represent and warrant this is my original work, and does not infringe or violate any rights of others. I warrant that I have obtained written permissions from the owner of any third party copyrighted material included in these files. I acknowledge that I retain ownership rights to the copyright of ...
Supplemental Digital Content
... extension. As positive control and reference an in-house cyclophilin (gCYC) qPCR was included. The specificities of the in house KRAS mutation assays varied between 0.1% and better than 0.01%. The final primer mixtures are shown in Table 1. All qPCR reactions were performed in a volume of 25 µl in ...
... extension. As positive control and reference an in-house cyclophilin (gCYC) qPCR was included. The specificities of the in house KRAS mutation assays varied between 0.1% and better than 0.01%. The final primer mixtures are shown in Table 1. All qPCR reactions were performed in a volume of 25 µl in ...
Molecular Immunology Circular Dichroism reveals evidence of coupling between immunoglobulin
... produced results that when compared across studies are inconsistent with one another as well as with the data presented in this paper (Vermeer and Norde, 2000; Vermeer et al., 1998; Jiskoot et al., 1991). Hence, the four isotypes studied here were more similar to one another with regards to secondar ...
... produced results that when compared across studies are inconsistent with one another as well as with the data presented in this paper (Vermeer and Norde, 2000; Vermeer et al., 1998; Jiskoot et al., 1991). Hence, the four isotypes studied here were more similar to one another with regards to secondar ...
Pairwise alignment
... gap creation and gap extension. • In BLAST, it is rarely necessary to change gap values from the default. ...
... gap creation and gap extension. • In BLAST, it is rarely necessary to change gap values from the default. ...
33_organelles.txt 3/25/2010 Limited proteolysis, phosphorylation
... disease. Their membranes contain hydrogen ion pumps and chloride ion channel proteins that maintain an interior pH of about 4.8, ideal for their proteasomes and other hydrolytic enzymes. Autophagy occurs in, FTP, these organelles responsible for enzymatic digestion of cell components and ingested ma ...
... disease. Their membranes contain hydrogen ion pumps and chloride ion channel proteins that maintain an interior pH of about 4.8, ideal for their proteasomes and other hydrolytic enzymes. Autophagy occurs in, FTP, these organelles responsible for enzymatic digestion of cell components and ingested ma ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.