Gene Section FST (follistatin) Atlas of Genetics and Cytogenetics
... et al., 1988) the first two, but not the third, are necessary for activin A binding (Keutmann et al., 2004; Harrington et al., 2006). Aside from activins, follistatin also binds several bone morphogenetic proteins (BMP) including BMP2, BMP4, BMP6 and BMP7 (Iemura et al., 1998; Glister et al., 2004). ...
... et al., 1988) the first two, but not the third, are necessary for activin A binding (Keutmann et al., 2004; Harrington et al., 2006). Aside from activins, follistatin also binds several bone morphogenetic proteins (BMP) including BMP2, BMP4, BMP6 and BMP7 (Iemura et al., 1998; Glister et al., 2004). ...
Substrate Specificity of Human Kallikreins 1 and 6
... same set of fluorescence resonance energy transfer (FRET) peptides that had been used for the analysis of the specificity of KLK1 mentioned above. Specifically, Angelo et al. reported that only Arg is accepted in the S1 subsite of KLK6 whereas S2 strongly prefers Phe and, to a lesser extent Leu, ove ...
... same set of fluorescence resonance energy transfer (FRET) peptides that had been used for the analysis of the specificity of KLK1 mentioned above. Specifically, Angelo et al. reported that only Arg is accepted in the S1 subsite of KLK6 whereas S2 strongly prefers Phe and, to a lesser extent Leu, ove ...
lipids and thyroid hormones - Deep Blue
... and oxidation of fatty acids support increased calorigenesis. Second, thyroid hormones simultaneously exert major anabolic effects; lipogenesis is stimulated, which seems paradoxical for the calorigenic role of the hormones. In thyrotoxic subjects, the energetically wasteful combination of lipid syn ...
... and oxidation of fatty acids support increased calorigenesis. Second, thyroid hormones simultaneously exert major anabolic effects; lipogenesis is stimulated, which seems paradoxical for the calorigenic role of the hormones. In thyrotoxic subjects, the energetically wasteful combination of lipid syn ...
BAG1, a negative regulator of Hsp70 chaperone activity, uncouples
... Permanently denatured [125I]RCMLA or unfolded β-gal was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addit ...
... Permanently denatured [125I]RCMLA or unfolded β-gal was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addit ...
Communication
... damage in vivo, we developed a quantitative assay for measuring levels of 3-nitrotyrosine. The method combines gas chromatography with stable isotope dilution mass spectrometry (GC-MS). Using this assay, we first investigated the relative yields of protein-bound oxidation products in bovine serum al ...
... damage in vivo, we developed a quantitative assay for measuring levels of 3-nitrotyrosine. The method combines gas chromatography with stable isotope dilution mass spectrometry (GC-MS). Using this assay, we first investigated the relative yields of protein-bound oxidation products in bovine serum al ...
Isolation of drug target protein
... MTX (Methotrexate) is an anti metabolite and anti folate drug. It is used in treatment of cancer and autoimmune diseases. We immobilized MTX derivatives on FG beads to capture target molecules in HeLa cell extracts. MTX derivatives Result 1. FG beads captured DFHR which is an enzyme that reduces dih ...
... MTX (Methotrexate) is an anti metabolite and anti folate drug. It is used in treatment of cancer and autoimmune diseases. We immobilized MTX derivatives on FG beads to capture target molecules in HeLa cell extracts. MTX derivatives Result 1. FG beads captured DFHR which is an enzyme that reduces dih ...
MDM2 inhibit p300-mediated p53 acetylation and activation by
... Whether the inhibition requires the association of MDM2 with p53 compare wild-type MDM2 with its N-terminally deleted form lacking amino acid 58-89 (p53 binding domain) ...
... Whether the inhibition requires the association of MDM2 with p53 compare wild-type MDM2 with its N-terminally deleted form lacking amino acid 58-89 (p53 binding domain) ...
Kinetic analysis of retroviral proteases by HELGA EIZERT
... spumavirus genus. Although foamy viruses (FVs) are often highly cytopathic in tissue culture, they are not clearly associated with any disease. FVs are endemic in many vertebrates, including cows, domestic and feral cats, horses, and all primates examined other than humans. It is remarkable that the ...
... spumavirus genus. Although foamy viruses (FVs) are often highly cytopathic in tissue culture, they are not clearly associated with any disease. FVs are endemic in many vertebrates, including cows, domestic and feral cats, horses, and all primates examined other than humans. It is remarkable that the ...
Development of prokaryotic cell-free systems for synthetic
... 1987) to encourage as much protein production as possible. Completely “synthetic” cellfree systems from purified components (Shimizu et al., 2001) have also been developed for hard-to-produce proteins. There has been a recent resurgence of using cell-free systems as a fundamental tool. An overview c ...
... 1987) to encourage as much protein production as possible. Completely “synthetic” cellfree systems from purified components (Shimizu et al., 2001) have also been developed for hard-to-produce proteins. There has been a recent resurgence of using cell-free systems as a fundamental tool. An overview c ...
Presentazione di PowerPoint
... groups are specific combinations of atoms and confer typical features to the molecules. ...
... groups are specific combinations of atoms and confer typical features to the molecules. ...
Prevention of Mitochondrial Oxidative Damage as a
... NADP-dependent isocitrate dehydrogenase and through a ⌬H⫹-dependent transhydrogenase (17). Within the mitochondrial phospholipid bilayer, the fat-soluble antioxidants vitamin E and Coenzyme Q both prevent lipid peroxidation, while Coenzyme Q also recycles vitamin E and is itself regenerated by the ...
... NADP-dependent isocitrate dehydrogenase and through a ⌬H⫹-dependent transhydrogenase (17). Within the mitochondrial phospholipid bilayer, the fat-soluble antioxidants vitamin E and Coenzyme Q both prevent lipid peroxidation, while Coenzyme Q also recycles vitamin E and is itself regenerated by the ...
Amino acids - Zanichelli
... groups are specific combinations of atoms and confer typical features to the molecules. ...
... groups are specific combinations of atoms and confer typical features to the molecules. ...
Design and Evaluation of Hydrophobic Ion
... efficiency. This approach offers several advantages including (i) alteration the aqueous solubility of a hydrophilic protein molecule in a reversible manner, (ii) higher solubility of complex in the lipid phase which may result in higher permeation across cell membrane, (iii) enhanced encapsulation ...
... efficiency. This approach offers several advantages including (i) alteration the aqueous solubility of a hydrophilic protein molecule in a reversible manner, (ii) higher solubility of complex in the lipid phase which may result in higher permeation across cell membrane, (iii) enhanced encapsulation ...
An introduction to informatics - Swiss
... Dear Sir/Madame, I have a question concerning selection of data from UniProt protein database. I wonder if there are any examples of two or more protein entries, which concern exactly the same protein of two or more individuals representing the same species. In other words, I would like to know, if ...
... Dear Sir/Madame, I have a question concerning selection of data from UniProt protein database. I wonder if there are any examples of two or more protein entries, which concern exactly the same protein of two or more individuals representing the same species. In other words, I would like to know, if ...
Enzymes:The Catalysts of Life I
... reasonable rates. Since the energy content of a given molecule must exceed EA before that molecule is capable of undergoing reaction, the only way a reaction involving metastable reactants will proceed at an appreciable rate is to increase the proportion of molecules with sufficient energy. This can ...
... reasonable rates. Since the energy content of a given molecule must exceed EA before that molecule is capable of undergoing reaction, the only way a reaction involving metastable reactants will proceed at an appreciable rate is to increase the proportion of molecules with sufficient energy. This can ...
PDF - Geoff McFadden`s Lab
... • Debate over whether endosymbiont was red or green alga continues159,160. • Human trials of anti-apicoplast malaria drug 89. ...
... • Debate over whether endosymbiont was red or green alga continues159,160. • Human trials of anti-apicoplast malaria drug 89. ...
Hydrolysis of Bradykinin by Angiotensin
... (HEPES) was purchased from Calbiochem. The dipeptides, serylproline (Ser-Pro), phenylalanylarginine (PheArg), and Gly-Phe, were purchased from Fox Chemical Company. The concentrations of standard solutions of the peptides were determined by amino acid analysis following acid hydrolysis. The syntheti ...
... (HEPES) was purchased from Calbiochem. The dipeptides, serylproline (Ser-Pro), phenylalanylarginine (PheArg), and Gly-Phe, were purchased from Fox Chemical Company. The concentrations of standard solutions of the peptides were determined by amino acid analysis following acid hydrolysis. The syntheti ...
An Introduction to Enzyme Science
... integrate diverse input signals, thereby generating the most appropriate changes in catalytic activity. An interaction is said to be allosteric if binding of a low-molecular weight substance results in a metabolically significant conformational change. In most cases, modulating effects are negative ...
... integrate diverse input signals, thereby generating the most appropriate changes in catalytic activity. An interaction is said to be allosteric if binding of a low-molecular weight substance results in a metabolically significant conformational change. In most cases, modulating effects are negative ...
Kinetic Assay of Human Pepsin with Albumin
... one-to-one molar ratio. The value of k, the stability constant, was 3.72 x i0, which indicates that very little free dye or albumin is present when the two compounds are allowed to react in unimolar proportions. One of the curves is illustrated in Figure 1. Spectral properties of the complex. In Fig ...
... one-to-one molar ratio. The value of k, the stability constant, was 3.72 x i0, which indicates that very little free dye or albumin is present when the two compounds are allowed to react in unimolar proportions. One of the curves is illustrated in Figure 1. Spectral properties of the complex. In Fig ...
Sex-based individual variation of snake venom proteome among
... proteome variation among the venoms of this litter as well as the sex-based differences observed by both SDS-PAGE and 2D-PAGE we decided to compare the individual venom samples by measuring their proteolytic activities on protein and peptide substrates. At first we determined the plasma coagulant ac ...
... proteome variation among the venoms of this litter as well as the sex-based differences observed by both SDS-PAGE and 2D-PAGE we decided to compare the individual venom samples by measuring their proteolytic activities on protein and peptide substrates. At first we determined the plasma coagulant ac ...
... (Figure 1a) could be due to the presence of elastin, another stromal protein that is commonly found in collagen isolates due to the interlinking between these two proteins (Foster, 2013). After 1 h of hydrolysis with each enzyme, collagen extracted from fins and arms showed several bands with lower ...
... The pure isomeric formylvinylporphyrins were deesterified as described for diformyldeuteroporphyrin DTBE. It is especially advantageous to use tertiary butyl esters in situations such as this because they can be removed in the absence of water. Hydration of vinyl groups has long been a problem durin ...
Prediction of Folding, Stability and Structure of Proteins from Amino
... for the synthesis of a RNA molecule. This process is called transcription because during this phase of gene expression a transfer of information from one nucleic acid type to another occurs. Next, the RNA molecule is translated into a protein sequence. The RNA that is translated into a protein is ca ...
... for the synthesis of a RNA molecule. This process is called transcription because during this phase of gene expression a transfer of information from one nucleic acid type to another occurs. Next, the RNA molecule is translated into a protein sequence. The RNA that is translated into a protein is ca ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.