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031607
031607

... – High specificity and efficiency relative to inorganic catalysts, for example – Participate in reactions, but no net change – Lower the activation energy – Do not change equilibrium (get there faster) ...
CHEM523 Test 1
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No Slide Title

... In proline, the side chain is connected to the backbone at two places: the C and the N. Therefore, it is not an amino acid, but an imino acid. Unless it is the N-terminal residue, proline does not have a backbone proton, and thus is not good for helices and strands. Due to the extra covalent bond, ...
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... in a different manner than it does in the wildtype enzyme. The result is that the double bond of the acrylamide rather than the amide carbonyl carbon is adjacent to the active site cysteine. This demonstrates the role of the hydrogen bond between E142 Oε2 and the substrate amino group in positioning ...
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HIV Protease (H1415) - Datasheet - Sigma

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... Lethal illness can be caused by the malfunction of just one type of enzyme out of the thousands of types present in our bodies. E.g., the disease phenylketonuria (PKU) results from a mutation of a single amino acid in the enzyme phenylalanine hydroxylase, which catalyzes the first step in the degrad ...
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Enzyme - Northwest ISD Moodle
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... Enzymes aren’t used up • Enzymes are not changed by the reaction – used only temporarily – re-used again for the same reaction with other molecules – very little enzyme needed to help in many reactions substrate active site ...
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Catalytic triad



A catalytic triad refers to the three amino acid residues that function together at the centre of the active site of some hydrolase and transferase enzymes (e.g. proteases, amidases, esterases, acylases, lipases and β-lactamases). An Acid-Base-Nucleophile triad is a common motif for generating a nucleophilic residue for covalent catalysis. The residues form a charge-relay network to polarise and activate the nucleophile, which attacks the substrate, forming a covalent intermediate which is then hydrolysed to regenerate free enzyme. The nucleophile is most commonly a serine or cysteine amino acid, but occasionally threonine. Because enzymes fold into complex three-dimensional structures, the residues of a catalytic triad can be far from each other along the amino-acid sequence (primary structure), however, they are brought close together in the final fold.As well as divergent evolution of function (and even the triad's nucleophile), catalytic triads show some of the best examples of convergent evolution. Chemical constraints on catalysis have led to the same catalytic solution independently evolving in at least 23 separate superfamilies. Their mechanism of action is consequently one of the best studied in biochemistry.
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