Identification and analysis of new phloem proteins from
... The organelles that can be found in mature SE (mitochondria, plastids, endoplasmic reticulum) appear to be anchored to a membrane system that probably also protects some of the SE proteins from the bulk flow (Ehlers et al., 2000). The ER in SE is modified and is proposed to function as a rail system ...
... The organelles that can be found in mature SE (mitochondria, plastids, endoplasmic reticulum) appear to be anchored to a membrane system that probably also protects some of the SE proteins from the bulk flow (Ehlers et al., 2000). The ER in SE is modified and is proposed to function as a rail system ...
The large universal Pantoea plasmid LPP-1 plays a diversification
... The various ecological niches occupied by Pantoea species including both plant and animal hosts, and their distinctive lifestyles as epi- and endophytes, plantgrowth promoters, biological control agents, or as pathogens of plant and animal hosts are indicative of extensive diversification within the ...
... The various ecological niches occupied by Pantoea species including both plant and animal hosts, and their distinctive lifestyles as epi- and endophytes, plantgrowth promoters, biological control agents, or as pathogens of plant and animal hosts are indicative of extensive diversification within the ...
Pseudomonas aeruginosa Anaerobic Respiration in Biofilms
... formation and cell viability, we grew biofilms in LBN under anaerobic conditions using a panel of mutant strains designed to test genetically whether NO killed the rhlR mutant bacteria. Figure 3E shows that most of the anaerobic ⌬rhlR biofilm bacteria were dead, consistent with the results described ...
... formation and cell viability, we grew biofilms in LBN under anaerobic conditions using a panel of mutant strains designed to test genetically whether NO killed the rhlR mutant bacteria. Figure 3E shows that most of the anaerobic ⌬rhlR biofilm bacteria were dead, consistent with the results described ...
Alternative mRNA Splicing Generates the Two
... isolated from spinach and Arabidopsis thaliana indicates that the two polypeptides of rubisco activase arise from alternative splicing of a common pre-mRNA. In spinach, two 5’ splice sites are used in processing a single 137nucleotide intron near the 3‘ end of the primary transcript. This intron was ...
... isolated from spinach and Arabidopsis thaliana indicates that the two polypeptides of rubisco activase arise from alternative splicing of a common pre-mRNA. In spinach, two 5’ splice sites are used in processing a single 137nucleotide intron near the 3‘ end of the primary transcript. This intron was ...
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... transport activity in the CAM requires the presence of the substrate itself, the eggshell. Chick embryos which develop in long-term shell-less cultures in vitro fail to exhibit the characteristic age-specific onset of calcium transport in their CAM. Biochemical analysis (Tuan, 1980a) revealed that t ...
... transport activity in the CAM requires the presence of the substrate itself, the eggshell. Chick embryos which develop in long-term shell-less cultures in vitro fail to exhibit the characteristic age-specific onset of calcium transport in their CAM. Biochemical analysis (Tuan, 1980a) revealed that t ...
Promiscuity of the Euonymus Carbohydrate-Binding Domain
... A detailed analysis of the EUL sequences in dicot plants revealed that most dicot species such as Arabidopsis only have one or two genes encoding a single domain EUL sequence with a long unrelated N-terminal domain (type S3). In contrast, monocot and lower plant species have a whole set of EUL seque ...
... A detailed analysis of the EUL sequences in dicot plants revealed that most dicot species such as Arabidopsis only have one or two genes encoding a single domain EUL sequence with a long unrelated N-terminal domain (type S3). In contrast, monocot and lower plant species have a whole set of EUL seque ...
scf and cullin/ring h2-based
... inhibitor Sic1. Sic1 is normally destroyed as wild-type cells progress from G1 to S phase but persists indefinitely in the cdc mutants. Subsequently, it was shown that skp1ts mutants have a similar phenotype (Bai et al 1996). yCdc34 was a logical candidate for a Sic1 destabilizing factor because it ...
... inhibitor Sic1. Sic1 is normally destroyed as wild-type cells progress from G1 to S phase but persists indefinitely in the cdc mutants. Subsequently, it was shown that skp1ts mutants have a similar phenotype (Bai et al 1996). yCdc34 was a logical candidate for a Sic1 destabilizing factor because it ...
Acquisition, Conservation, and Loss of Dual
... The dual-targeting ability of a variety of proteins from Physcomitrella patens, rice (Oryza sativa), and Arabidopsis (Arabidopsis thaliana) was tested to determine when dual targeting arose and to what extent it was conserved in land plants. Overall, the targeting ability of over 80 different protei ...
... The dual-targeting ability of a variety of proteins from Physcomitrella patens, rice (Oryza sativa), and Arabidopsis (Arabidopsis thaliana) was tested to determine when dual targeting arose and to what extent it was conserved in land plants. Overall, the targeting ability of over 80 different protei ...
recommendations for tissue preparation and formalin or
... alter the quality of molecular analytes. A recent report recommends that tissue not be fixed for more than 36hrs to avoid overfixation and excessive cross-linking. We have also found that extended fixation can cause excessive brittleness of paraffin-embedded mouse tissues, which are inherently “ ...
... alter the quality of molecular analytes. A recent report recommends that tissue not be fixed for more than 36hrs to avoid overfixation and excessive cross-linking. We have also found that extended fixation can cause excessive brittleness of paraffin-embedded mouse tissues, which are inherently “ ...
Document
... The body of the lophophore is entirely ciliated, except possibly for mucous cells. The outer surfaces of the filaments are entirely ciliated, the cilia being in four tracts: frontal, abfrontal and paired lateral. The ciliary feeding mechanism of M. cranium has been briefly described (Atkins, 1956) a ...
... The body of the lophophore is entirely ciliated, except possibly for mucous cells. The outer surfaces of the filaments are entirely ciliated, the cilia being in four tracts: frontal, abfrontal and paired lateral. The ciliary feeding mechanism of M. cranium has been briefly described (Atkins, 1956) a ...
Type III secretion: The bacteria-eukaryotic cell
... chaperone, helps the hydrophobic translocators to integrate in the eukaryotic plasma membrane and to form there a pore (Fig. 2). This model is supported by similar observations made in P. aeruginosa [25], Shigella [26,27] and EPEC [28], but different aspects still need to be challenged. Firstly, the ...
... chaperone, helps the hydrophobic translocators to integrate in the eukaryotic plasma membrane and to form there a pore (Fig. 2). This model is supported by similar observations made in P. aeruginosa [25], Shigella [26,27] and EPEC [28], but different aspects still need to be challenged. Firstly, the ...
Benzoate mediates the simultaneous repression of anaerobic 4
... cells were used as inoculum (data not shown). A notable observation is the unaltered utilization hierarchy with the ternary mixture, i.e. benzoate over succinate and 4methylbenzoate, mirroring the preferences observed with respective binary mixtures (Figure 1a–c). Transcript and protein dynamics (te ...
... cells were used as inoculum (data not shown). A notable observation is the unaltered utilization hierarchy with the ternary mixture, i.e. benzoate over succinate and 4methylbenzoate, mirroring the preferences observed with respective binary mixtures (Figure 1a–c). Transcript and protein dynamics (te ...
Tracing the Archaeal Origins of Eukaryotic Membrane
... similarity to the Gtr1_RagA/PF04670 domain (supplementary table S1, Supplementary Material online). Proteins with this domain comprise atypical members of the Ras superfamily functioning in target of rapamycin complex 1 (TORC1) signaling at the lysosome/vacuole (Sekiguchi et al. 2001; Kim et al. 200 ...
... similarity to the Gtr1_RagA/PF04670 domain (supplementary table S1, Supplementary Material online). Proteins with this domain comprise atypical members of the Ras superfamily functioning in target of rapamycin complex 1 (TORC1) signaling at the lysosome/vacuole (Sekiguchi et al. 2001; Kim et al. 200 ...
Proteolytic and other metabolic pathways in lysosomes
... N-acetylhexosamines. The final links to the protein may be tence of a number of activator proteins that dramatically 0-glycosidic links to serine or threonine, or N-glycosidic enhance the action of certain of the enzymes on the lipid links to asparagine. The 0-glycosidic links are hydrolysed substra ...
... N-acetylhexosamines. The final links to the protein may be tence of a number of activator proteins that dramatically 0-glycosidic links to serine or threonine, or N-glycosidic enhance the action of certain of the enzymes on the lipid links to asparagine. The 0-glycosidic links are hydrolysed substra ...
The Evolution of SMC Proteins: Phylogenetic Analysis and Structural
... The SMC proteins are found in nearly all living organisms examined, where they play crucial roles in mitotic chromosome dynamics, regulation of gene expression, and DNA repair. We have explored the phylogenetic relationships of SMC proteins from prokaryotes and eukaryotes, as well as their relations ...
... The SMC proteins are found in nearly all living organisms examined, where they play crucial roles in mitotic chromosome dynamics, regulation of gene expression, and DNA repair. We have explored the phylogenetic relationships of SMC proteins from prokaryotes and eukaryotes, as well as their relations ...
Systematic Characterisation of Cellular Localisation and
... Many methods have been developed for predicting which peptides are presented by MHC class I and II molecules [1–4], since these peptides could be used for vaccine or diagnostic purposes. The focus of the available methods is, however, to identify the optimal peptides from provided protein sequences, ...
... Many methods have been developed for predicting which peptides are presented by MHC class I and II molecules [1–4], since these peptides could be used for vaccine or diagnostic purposes. The focus of the available methods is, however, to identify the optimal peptides from provided protein sequences, ...
Proteomic Approach to Identify Novel
... location of the respiratory chain that reoxidizes NAD⫹, transfers electrons to molecular O2, and generates a proton gradient across the inner mitochondrial membrane, and they are the site of ADP phosphorylation by the ATP synthase complex. Furthermore, mitochondria are involved in several anabolic r ...
... location of the respiratory chain that reoxidizes NAD⫹, transfers electrons to molecular O2, and generates a proton gradient across the inner mitochondrial membrane, and they are the site of ADP phosphorylation by the ATP synthase complex. Furthermore, mitochondria are involved in several anabolic r ...
Site-selective incorporation and ligation of
... diols encountered in carbohydrates.24 Care must be taken when using this method however as other amino acids can be oxidised under certain conditions (cysteine and methionine residues in particular).23 Therefore, the reaction is usually performed with or quenched by another component such as excess ...
... diols encountered in carbohydrates.24 Care must be taken when using this method however as other amino acids can be oxidised under certain conditions (cysteine and methionine residues in particular).23 Therefore, the reaction is usually performed with or quenched by another component such as excess ...
Sample pages 1 PDF
... tion of tissues. Hence, it is always recommended that a fresh formaldehyde solution is made from paraformaldehyde (PFA) powder and used immediately. Glutaraldehyde was introduced by Sabatini et al. in 1963 as an especially useful fixative for ultrastructural studies [22]. This compound has two aldeh ...
... tion of tissues. Hence, it is always recommended that a fresh formaldehyde solution is made from paraformaldehyde (PFA) powder and used immediately. Glutaraldehyde was introduced by Sabatini et al. in 1963 as an especially useful fixative for ultrastructural studies [22]. This compound has two aldeh ...
PDF Datastream - Brown Digital Repository
... RsaA- using Q-TOF or LTQ-Orbitrap……………………...............................................81 Figure 3.8: Functional classification of total 91 putative surface-exposed proteins identified using both Q-TOF and LTQ-Orbitrap method..................................................82 Figure 3.9: Distribu ...
... RsaA- using Q-TOF or LTQ-Orbitrap……………………...............................................81 Figure 3.8: Functional classification of total 91 putative surface-exposed proteins identified using both Q-TOF and LTQ-Orbitrap method..................................................82 Figure 3.9: Distribu ...
Silaffins of Diatoms: From Applied Biotechnology to Biomedicine
... and can then self-immobilize to form silicon dioxide [30]. Using GOX-R5, the galvanic cell, a biosensor, was developed to control the concentration of glucose in eukaryotic cells. This method of immobilization can be applied to various types of biomolecules. A simple, universal technique for synthes ...
... and can then self-immobilize to form silicon dioxide [30]. Using GOX-R5, the galvanic cell, a biosensor, was developed to control the concentration of glucose in eukaryotic cells. This method of immobilization can be applied to various types of biomolecules. A simple, universal technique for synthes ...
Silaffins of Diatoms: From Applied Biotechnology to
... and can then self-immobilize to form silicon dioxide [30]. Using GOX-R5, the galvanic cell, a biosensor, was developed to control the concentration of glucose in eukaryotic cells. This method of immobilization can be applied to various types of biomolecules. A simple, universal technique for synthes ...
... and can then self-immobilize to form silicon dioxide [30]. Using GOX-R5, the galvanic cell, a biosensor, was developed to control the concentration of glucose in eukaryotic cells. This method of immobilization can be applied to various types of biomolecules. A simple, universal technique for synthes ...
The twin arginine protein transport pathway exports multiple
... potent inhibitor of cellulose synthesis. The toxin induces plant cell hypertrophy in expanding plant tissues and likely facilitates penetration of plant tissue by the pathogen (King et al., 1989; Healy et al., 2000; Scheible et al., 2003; Bischoff et al., 2009). The mechanism by which thaxtomin inhi ...
... potent inhibitor of cellulose synthesis. The toxin induces plant cell hypertrophy in expanding plant tissues and likely facilitates penetration of plant tissue by the pathogen (King et al., 1989; Healy et al., 2000; Scheible et al., 2003; Bischoff et al., 2009). The mechanism by which thaxtomin inhi ...
Formation of Enzymes of Autotrophic Metabolism
... Alcaligenes eutrophus is an obligate aerobic, facultative chemolithoautotrophic microorganism. During autotrophic growth on carbon dioxide and hydrogen, the latter substrate is activated by a soluble NAD+-linked hydrogenase (EC 1.12.1.2) and a membrane-bound hydrogenase unable to reduce pyridine nuc ...
... Alcaligenes eutrophus is an obligate aerobic, facultative chemolithoautotrophic microorganism. During autotrophic growth on carbon dioxide and hydrogen, the latter substrate is activated by a soluble NAD+-linked hydrogenase (EC 1.12.1.2) and a membrane-bound hydrogenase unable to reduce pyridine nuc ...
Bacterial microcompartment
Bacterial Microcompartments (BMCs) are organelles consisting of a protein shell that encloses enzymes and other proteins. BMCs are typically about 40-200 nanometers in diameter and are entirely made of proteins. The shell functions like a membrane, as it is selectively permeable. Other protein-based compartments found in bacteria and archaea include encapsulin nanocompartments and gas vesicles. Eukaryotes have also been observed to have proteinaceous organelles, such as the mysterious vault complex.