Do Legume Storage Proteins Play a Role in
... salt-soluble globulins of which the legumins are formed by two chains, linked by disulfide bonds. They are generally encoded by a large number of genes whose translation products are proteolytically processed to the final two-chain proteins. Legumins aggregate to form hexamers and are also known as ...
... salt-soluble globulins of which the legumins are formed by two chains, linked by disulfide bonds. They are generally encoded by a large number of genes whose translation products are proteolytically processed to the final two-chain proteins. Legumins aggregate to form hexamers and are also known as ...
Protein Targeting into the Complex Plastid of Cryptophytes
... amino acid of the signal peptide cleavage site of the nuclear-encoded proteins is in boldface. Note that all plastid proteins share a phenylalanine at their N-terminal transit peptide, which is not present in PPC proteins. Only the first 20 amino acids of the transit peptides are shown. ...
... amino acid of the signal peptide cleavage site of the nuclear-encoded proteins is in boldface. Note that all plastid proteins share a phenylalanine at their N-terminal transit peptide, which is not present in PPC proteins. Only the first 20 amino acids of the transit peptides are shown. ...
Thesis - u
... We are interested in understanding the molecular mechanisms involved in the targetedtransport of macromolecules from CC to the SE. The dimeric phloem lectins, also known as PP2s, for Phloem Proteins 2, have been proposed to be involved in the shuttling of glycoproteins from CC to the SE [5, 6]. Thes ...
... We are interested in understanding the molecular mechanisms involved in the targetedtransport of macromolecules from CC to the SE. The dimeric phloem lectins, also known as PP2s, for Phloem Proteins 2, have been proposed to be involved in the shuttling of glycoproteins from CC to the SE [5, 6]. Thes ...
Nedd8 processing enzymes inSchizosaccharomyces pombe
... that regulates many cellular processes including protein degradation, endocytosis, DNA repair and cell signalling. The protein called Nedd8 in humans and Rub1 in budding yeast is a ubiquitin-like modifier that is covalently conjugated to a lysine residue in the cullin subunits of SCF-type E3 ubiquit ...
... that regulates many cellular processes including protein degradation, endocytosis, DNA repair and cell signalling. The protein called Nedd8 in humans and Rub1 in budding yeast is a ubiquitin-like modifier that is covalently conjugated to a lysine residue in the cullin subunits of SCF-type E3 ubiquit ...
Short review - BioPublisher
... or more than one specific subcellular location(s) in order to play their biological roles, a process called protein targeting or sorting. Experimental approaches for identifying protein subcellular locations are widely exploited including isolation of organelles, green fluorescence tagging proteins, ...
... or more than one specific subcellular location(s) in order to play their biological roles, a process called protein targeting or sorting. Experimental approaches for identifying protein subcellular locations are widely exploited including isolation of organelles, green fluorescence tagging proteins, ...
The Identity of Proteins Associated with a Small Heat Shock Protein
... (from 50 ml of heat-stressed cells) was separated by two-dimensional gel electrophoresis and stained with Coomassie Blue. Polypeptides identified as heat-associated with Hsp16.6 and released by DnaK, DnaJ, and GrpE were excised from the gel and subjected to trypsin digestion according to Shevchenko ...
... (from 50 ml of heat-stressed cells) was separated by two-dimensional gel electrophoresis and stained with Coomassie Blue. Polypeptides identified as heat-associated with Hsp16.6 and released by DnaK, DnaJ, and GrpE were excised from the gel and subjected to trypsin digestion according to Shevchenko ...
Characterization of proteins secreted from a Type III secretion
... and eseD genes in the wild-type strain as described growth of bacteria in the macrophages (Okuda et al. previously (Tan et al. 2005) with a slight modification 2006). However, only scant information is available on using the suicide vector plasmid, pRE112 (Okuda et al. the pathogenicity of E. tarda. ...
... and eseD genes in the wild-type strain as described growth of bacteria in the macrophages (Okuda et al. previously (Tan et al. 2005) with a slight modification 2006). However, only scant information is available on using the suicide vector plasmid, pRE112 (Okuda et al. the pathogenicity of E. tarda. ...
Outer dense fibre protein 2 (ODF2) is a self
... to these fibres. Instead of being directly involved in the induction of progressive motility the ODF seem to have a modulating influence on sperm motility. They may be necessary for the maintenance of the elastic properties of the sperm tail and may provide tensile strength that is necessary to prot ...
... to these fibres. Instead of being directly involved in the induction of progressive motility the ODF seem to have a modulating influence on sperm motility. They may be necessary for the maintenance of the elastic properties of the sperm tail and may provide tensile strength that is necessary to prot ...
Light Modulates the Biosynthesis and
... Synechococcus strain. The eGFP fluorescence (green) indicates the subcellular localization of carboxysomes, and the endogenous chlorophyll fluorescence (red) shows the organization of thylakoid membranes. In addition, the specific DNA-staining dye 49,6-diaminophenylindole (DAPI) was used to image chrom ...
... Synechococcus strain. The eGFP fluorescence (green) indicates the subcellular localization of carboxysomes, and the endogenous chlorophyll fluorescence (red) shows the organization of thylakoid membranes. In addition, the specific DNA-staining dye 49,6-diaminophenylindole (DAPI) was used to image chrom ...
Xanthomonas campestris
... and dissemination of Xcc in infected tissue [9]. Some studies have reported the possibility of an increase in metabolism proteins during the infection process, which could be involved in bacterial pathogenicity [30]. Nevertheless, the functions of these genes in bacterial physiology and their relati ...
... and dissemination of Xcc in infected tissue [9]. Some studies have reported the possibility of an increase in metabolism proteins during the infection process, which could be involved in bacterial pathogenicity [30]. Nevertheless, the functions of these genes in bacterial physiology and their relati ...
Glutathionylation in the Photosynthetic Model Organism
... liters/min. Helium was used as cooling and fragmentation gas at a pressure of 6 ⫻ 10⫺7 mbar. Purification of Glutathionylated Peptides—After glutathionylation and G-25 gel filtration steps, the proteins were concentrated up to 8 –10 mg/ml using Microcon devices YM-10 (Millipore). 65 g of proteins w ...
... liters/min. Helium was used as cooling and fragmentation gas at a pressure of 6 ⫻ 10⫺7 mbar. Purification of Glutathionylated Peptides—After glutathionylation and G-25 gel filtration steps, the proteins were concentrated up to 8 –10 mg/ml using Microcon devices YM-10 (Millipore). 65 g of proteins w ...
Vitamin B12 (Cobalamin) - SpectraCell Laboratories
... cardiovascular disease and connective tissue abnormalities. Hypochlorhydria and gastrointestinal disturbances are frequently associated with vitamin B12 deficiency. ...
... cardiovascular disease and connective tissue abnormalities. Hypochlorhydria and gastrointestinal disturbances are frequently associated with vitamin B12 deficiency. ...
RbcL | Rubisco large subunit, form I and form II (50 µl) product
... This antibody is especially suitable for quantifying of Rubisco in plant and algal samples. Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants a ...
... This antibody is especially suitable for quantifying of Rubisco in plant and algal samples. Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants a ...
1. introduction - International Journal of Computer Applications
... Rajiv Gandhi Centre for Biotechnology, Thiruvanathapuram Kerala, India ...
... Rajiv Gandhi Centre for Biotechnology, Thiruvanathapuram Kerala, India ...
Differentially Expressed Soluble Proteins in Aortic Cells from
... spots were analyzed with Phoretix Software (Nonlinear Dynamics, version 6.01) to identify relative isoelectric point (pI) and molecular weight (MW). Individual spots were not identified on each gel. Only differentially expressed spots (WC vs. SR) identified by Phoretix Software were recorded. For th ...
... spots were analyzed with Phoretix Software (Nonlinear Dynamics, version 6.01) to identify relative isoelectric point (pI) and molecular weight (MW). Individual spots were not identified on each gel. Only differentially expressed spots (WC vs. SR) identified by Phoretix Software were recorded. For th ...
Ch 9 modified
... – Cells regulate the last stage of exocytosis (fusion) for most exocytic vesicles, to control when and how much material is released into the extracellular space and to control the delivery of membrane-associated proteins to the plasma membrane. – Controlled secretion is also called regulated secret ...
... – Cells regulate the last stage of exocytosis (fusion) for most exocytic vesicles, to control when and how much material is released into the extracellular space and to control the delivery of membrane-associated proteins to the plasma membrane. – Controlled secretion is also called regulated secret ...
Oxidative stress in bacteria and protein damage by reactive oxygen
... reaction is viewed as a “caged” process in which the active oxygen species is not released into the surrounding medium; it preferentially reacts with functional groups of amino acid residues at the metal-binding site. In this mechanism, it is assumed that the hydroxyl radical (·OH) is the reactive o ...
... reaction is viewed as a “caged” process in which the active oxygen species is not released into the surrounding medium; it preferentially reacts with functional groups of amino acid residues at the metal-binding site. In this mechanism, it is assumed that the hydroxyl radical (·OH) is the reactive o ...
Severa1 Proteins lmported into Chloroplasts Form
... An abundant chloroplast stromal protein has been implicated in the assembly of the photosynthetic enzyme ribulose-1,.Ç-bisphosphate carboxylase/oxygenase (Rubisco) (Barraclough and Ellis, 1980; Roy et al., 1982; Cannon, Wang, and Roy, 1986; Gatenby et al., 1988), and has been referred to as either t ...
... An abundant chloroplast stromal protein has been implicated in the assembly of the photosynthetic enzyme ribulose-1,.Ç-bisphosphate carboxylase/oxygenase (Rubisco) (Barraclough and Ellis, 1980; Roy et al., 1982; Cannon, Wang, and Roy, 1986; Gatenby et al., 1988), and has been referred to as either t ...
HydF as a scaffold protein in [FeFe] hydrogenase H
... showed that all three proteins are capable of binding FeS clusters [6,7]. In our most recent work, we have shown that cell extracts of E. coli in which all three Hyd proteins from C. acetobutylicum are expressed simultaneously can activate heterologously expressed HydA [8]. The in vitro activation o ...
... showed that all three proteins are capable of binding FeS clusters [6,7]. In our most recent work, we have shown that cell extracts of E. coli in which all three Hyd proteins from C. acetobutylicum are expressed simultaneously can activate heterologously expressed HydA [8]. The in vitro activation o ...
LysM, a widely distributed protein motif for binding to
... obtained in the range of pH 4–10. A similar fusion with the LysM domain of AcmD only bound at a pH below the pI of that domain (~pH 4) (G. Buist et al., unpublished), suggesting that positive charges play an important role in binding. Binding studies using the AcmA fusion protein and chemically trea ...
... obtained in the range of pH 4–10. A similar fusion with the LysM domain of AcmD only bound at a pH below the pI of that domain (~pH 4) (G. Buist et al., unpublished), suggesting that positive charges play an important role in binding. Binding studies using the AcmA fusion protein and chemically trea ...
No Slide Title
... Some secreted proteins undergo multiple cleavages Proinsulin - regulated secretion. PC2 and PC3 endoproteases - also in Kex2 family. Only found in cells with regulated secretion. Localized to secretory vesicles. ...
... Some secreted proteins undergo multiple cleavages Proinsulin - regulated secretion. PC2 and PC3 endoproteases - also in Kex2 family. Only found in cells with regulated secretion. Localized to secretory vesicles. ...
(GCKIII) proteins using a mechanism analogous to CCM3
... tissue-specific deletion in the vascular endothelium (6). A noncell autonomous role for CCM3 in neuroglia on the vasculature has also been uncovered in mouse recently, indicating that CCMs may arise in the central nervous system by the loss of CCM3 signaling in neural as well as endothelial populati ...
... tissue-specific deletion in the vascular endothelium (6). A noncell autonomous role for CCM3 in neuroglia on the vasculature has also been uncovered in mouse recently, indicating that CCMs may arise in the central nervous system by the loss of CCM3 signaling in neural as well as endothelial populati ...
Proteomics studies of post-translational modifications in plants
... pathogen treatment, a suspension culture of Arabidopsis was pulse-labelled with 32P and the phosphorylated proteins were visualized by 2DE. One of these proteins, AtPhos43, that was phosphorylated within minutes after treatment with flagellin or chitin fragments was identified by nanoESI MS/MS (Peck ...
... pathogen treatment, a suspension culture of Arabidopsis was pulse-labelled with 32P and the phosphorylated proteins were visualized by 2DE. One of these proteins, AtPhos43, that was phosphorylated within minutes after treatment with flagellin or chitin fragments was identified by nanoESI MS/MS (Peck ...
Bacterial microcompartment
Bacterial Microcompartments (BMCs) are organelles consisting of a protein shell that encloses enzymes and other proteins. BMCs are typically about 40-200 nanometers in diameter and are entirely made of proteins. The shell functions like a membrane, as it is selectively permeable. Other protein-based compartments found in bacteria and archaea include encapsulin nanocompartments and gas vesicles. Eukaryotes have also been observed to have proteinaceous organelles, such as the mysterious vault complex.