Plant serine/arginine-rich proteins and their role in pre
... www.tigr.org/tdb/e2k1/ath1/altsplicing/splicing_variations. shtml). Retention of introns in plants appears to be fairly common. About 15% of alternatively spliced transcripts have introns retained either in their untranslated regions (190) or in the protein coding region (218 genes) or both (22) [8] ...
... www.tigr.org/tdb/e2k1/ath1/altsplicing/splicing_variations. shtml). Retention of introns in plants appears to be fairly common. About 15% of alternatively spliced transcripts have introns retained either in their untranslated regions (190) or in the protein coding region (218 genes) or both (22) [8] ...
Common infection strategies of plant and animal pathogenic bacteria
... Once the bacteria are close to a host cell, they start to inject effector proteins into the cytosol of the eukaryotic ...
... Once the bacteria are close to a host cell, they start to inject effector proteins into the cytosol of the eukaryotic ...
Morphological and degenerative changes in a suspected post
... Information on degenerative bony changes in the articular surfaces of the joint secondary to BMC has not previously been reported and is of importance to clinicians as such information highlights likely long term morphological and functional alterations from untreated posttraumatic BMC. Fractures an ...
... Information on degenerative bony changes in the articular surfaces of the joint secondary to BMC has not previously been reported and is of importance to clinicians as such information highlights likely long term morphological and functional alterations from untreated posttraumatic BMC. Fractures an ...
A biofilm-forming marine bacterium producing proteins
... another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates while the two others consist mainly in a majority of proteins. Gel electrophoresis analysis showed different EPS proteins composition with molecular weight ...
... another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates while the two others consist mainly in a majority of proteins. Gel electrophoresis analysis showed different EPS proteins composition with molecular weight ...
The proteome of the infectious bronchitis virus Beau
... each replicate (N – 144, 81 and 50; S – 21, 14 and 12; M – 11, 7 and 7). The fourth viral structural protein, E, the replicase proteins and the lineage-specific accessory proteins could not be identified. The total number of proteins identified in each preparation was 399, 289 and 273, respectively. ...
... each replicate (N – 144, 81 and 50; S – 21, 14 and 12; M – 11, 7 and 7). The fourth viral structural protein, E, the replicase proteins and the lineage-specific accessory proteins could not be identified. The total number of proteins identified in each preparation was 399, 289 and 273, respectively. ...
The role of cytosolic proteins in the insertion of tail
... nascent chain to the Sec61 protein-conducting channel (Rapoport, 2007). The well characterized co-translational pathway is, however, not accessible to the large and diverse group of membrane proteins that are classified as tail-anchored (TA) proteins. TA proteins consist of a cytosolic N-terminal do ...
... nascent chain to the Sec61 protein-conducting channel (Rapoport, 2007). The well characterized co-translational pathway is, however, not accessible to the large and diverse group of membrane proteins that are classified as tail-anchored (TA) proteins. TA proteins consist of a cytosolic N-terminal do ...
Full Text - Molecular Biology and Evolution
... F(2,61)¼ 21.07; P ¼ 1.1107; x2 ¼ 0.149). Filtering for proteins shared by eukaryotes and at least one of the prokaryotic domains produces the results in figure 3B. While prokaryotes are largely unaltered and the difference between unicellular and multicellular eukaryotes remains, the effect become ...
... F(2,61)¼ 21.07; P ¼ 1.1107; x2 ¼ 0.149). Filtering for proteins shared by eukaryotes and at least one of the prokaryotic domains produces the results in figure 3B. While prokaryotes are largely unaltered and the difference between unicellular and multicellular eukaryotes remains, the effect become ...
Intracellular Protein Degradation
... inactivation’ that possibly involves their phosphorylation. However this regulated mechanism for vacuolar degradation is limited only to a small and specific group of proteins (see for example Ref. 18; reviewed in Ref. 19). More recent studies have shown that at least for stress-induced macroautopha ...
... inactivation’ that possibly involves their phosphorylation. However this regulated mechanism for vacuolar degradation is limited only to a small and specific group of proteins (see for example Ref. 18; reviewed in Ref. 19). More recent studies have shown that at least for stress-induced macroautopha ...
Proteomic Strategies to Analyze Cell
... The use of Proteomic strategies in the discovery process is imperative since post-transcriptional modification can produce dramatic changes in protein levels and activity that are invisible to DNA arrays. The introduction of new and improved proteomics solutions with increased sensitivity, specifici ...
... The use of Proteomic strategies in the discovery process is imperative since post-transcriptional modification can produce dramatic changes in protein levels and activity that are invisible to DNA arrays. The introduction of new and improved proteomics solutions with increased sensitivity, specifici ...
putative mineral-specific proteins synthesized by a
... several of the protein spots predominately detected when Fe(III) was the terminal electron acceptor. Among those identified were proteins involved in metal reduction, protein transport and secretion, polysaccharide biosynthesis and export, and hypothetical proteins with unknown functions. A comparis ...
... several of the protein spots predominately detected when Fe(III) was the terminal electron acceptor. Among those identified were proteins involved in metal reduction, protein transport and secretion, polysaccharide biosynthesis and export, and hypothetical proteins with unknown functions. A comparis ...
The epidermal intermediate filament proteins of
... (antisense, nt 1549 ± 1515) 5'-CCAAGTGGACGTTTAGAATTCCTTTCTTTCTACTC-3' for D. Primers were designed to create unique restriction sites flanking the 5'- and 3'-ends of the coding sequences. PCR products were digested with the appropriate enzymes, gel purified and ligated in frame into pET23a vector DN ...
... (antisense, nt 1549 ± 1515) 5'-CCAAGTGGACGTTTAGAATTCCTTTCTTTCTACTC-3' for D. Primers were designed to create unique restriction sites flanking the 5'- and 3'-ends of the coding sequences. PCR products were digested with the appropriate enzymes, gel purified and ligated in frame into pET23a vector DN ...
KUMASI™ stabilized colloidal stain for polyacrylamide gels
... Silver stain is inarguably the most sensitive stain. It is mimics a photographic process in which the latent image is “developed” by the reduction of silver ions. However, since the binding of silver ions to proteins is highly dependent on amino acid composition, all proteins are not stained equally ...
... Silver stain is inarguably the most sensitive stain. It is mimics a photographic process in which the latent image is “developed” by the reduction of silver ions. However, since the binding of silver ions to proteins is highly dependent on amino acid composition, all proteins are not stained equally ...
The Methylosome, a 20S Complex Containing JBP1 and pICln
... in vivo. SMN binds preferentially and directly to the symmetrical dimethylarginine (sDMA)-modified arginineand glycine-rich (RG-rich) domains of SmD1 and SmD3. We found that the unmodified, but not the sDMAmodified, RG domains of SmD1 and SmD3 associate with a 20S methyltransferase complex, termed t ...
... in vivo. SMN binds preferentially and directly to the symmetrical dimethylarginine (sDMA)-modified arginineand glycine-rich (RG-rich) domains of SmD1 and SmD3. We found that the unmodified, but not the sDMAmodified, RG domains of SmD1 and SmD3 associate with a 20S methyltransferase complex, termed t ...
Molecular mechanisms of Salmonella invasion
... structural components which assist in the translocation process, since non-polar mutations in the spa genes abolish Salmonella entry into tissue culture cells, and prevent the secretion [16] of type III target proteins. Outer membrane proteins Three proteins may be localized in Salmonella outer memb ...
... structural components which assist in the translocation process, since non-polar mutations in the spa genes abolish Salmonella entry into tissue culture cells, and prevent the secretion [16] of type III target proteins. Outer membrane proteins Three proteins may be localized in Salmonella outer memb ...
PDF document
... Cellulose is the main structural component of plant cell wall and thus the most abundant carbohydrate in nature. However, extracting the energy from this abundant source is limited by its recalcitrant nature. The hydrolysis of plant cell wall requires synergystic action of different enzymes, includi ...
... Cellulose is the main structural component of plant cell wall and thus the most abundant carbohydrate in nature. However, extracting the energy from this abundant source is limited by its recalcitrant nature. The hydrolysis of plant cell wall requires synergystic action of different enzymes, includi ...
occasional article intracellular protein degradation: from a vague
... to RNA and translated to proteins, but how proteins are degraded has remained a neglected research area. With the discovery of the lysosome by Christian de Duve it was assumed that cellular proteins are degraded within this organelle. Yet, several independent lines of experimental evidence strongly ...
... to RNA and translated to proteins, but how proteins are degraded has remained a neglected research area. With the discovery of the lysosome by Christian de Duve it was assumed that cellular proteins are degraded within this organelle. Yet, several independent lines of experimental evidence strongly ...
The Bacterial Phosphotransferase System: Structure, Function
... PTS auxiliary proteins such as the fructose repressor, FruR, and the Mlc transcription factor are believed to control transcription of the PTS genetic apparatus as well as of genes encoding central pathways of carbon metabolism in enteric bacteria (Plumbridge, 1999; Saier and Ramseier, 1996). These ...
... PTS auxiliary proteins such as the fructose repressor, FruR, and the Mlc transcription factor are believed to control transcription of the PTS genetic apparatus as well as of genes encoding central pathways of carbon metabolism in enteric bacteria (Plumbridge, 1999; Saier and Ramseier, 1996). These ...
Cloning, Expression and Interaction Studies of the Potential
... ____________________________________ Sabine Heinhorst, Ph.D., Chair Department of Chemistry and Biochemistry ...
... ____________________________________ Sabine Heinhorst, Ph.D., Chair Department of Chemistry and Biochemistry ...
The plastid division proteins, FtsZ1 and FtsZ2, differ in their
... PQE31. In this plasmid the protein is fused to a small histidine tag, and such small tags have been shown not to interfere with the biological activities of bacterial FtsZ [19,20]. In the absence of IPTG, the proteins were not expressed, as shown by Western blot with anti-histidine antibodies (Figur ...
... PQE31. In this plasmid the protein is fused to a small histidine tag, and such small tags have been shown not to interfere with the biological activities of bacterial FtsZ [19,20]. In the absence of IPTG, the proteins were not expressed, as shown by Western blot with anti-histidine antibodies (Figur ...
Plastid-Targeting Peptides from the
... (Emanuelsson et al. 1999) was used in combination with a multiple alignment of homologous proteins from other eukaryotes and eubacteria. In instances where ChloroP was unable to predict a transit peptide, or if the cleavage site of the transit peptide fell within the conserved portion of the mature ...
... (Emanuelsson et al. 1999) was used in combination with a multiple alignment of homologous proteins from other eukaryotes and eubacteria. In instances where ChloroP was unable to predict a transit peptide, or if the cleavage site of the transit peptide fell within the conserved portion of the mature ...
lecture 8
... but recent study shows only a portion of those are completely chaperonin-dependent Belongs to so-called Group I chaperonins which includes evolutionarily-related bacterial GroEL, mitochondrial Hsp60, and chloroplast Rubisco subunit-binding protein (Rubisco is most abundant protein on earth and req ...
... but recent study shows only a portion of those are completely chaperonin-dependent Belongs to so-called Group I chaperonins which includes evolutionarily-related bacterial GroEL, mitochondrial Hsp60, and chloroplast Rubisco subunit-binding protein (Rubisco is most abundant protein on earth and req ...
Proteomic analyses of the time course responses of mice infected
... markedly greater intensity than average were identified using MALDI-TOF MS. The results of the protein identification analysis are shown in Table 3. These data revealed that multiple immunogenic proteins at different locations on 2DE-immunoblotting showed diverse Mr and pI values and were correlated ...
... markedly greater intensity than average were identified using MALDI-TOF MS. The results of the protein identification analysis are shown in Table 3. These data revealed that multiple immunogenic proteins at different locations on 2DE-immunoblotting showed diverse Mr and pI values and were correlated ...
The trans-Golgi network GRIP-domain proteins form α
... Members of the family of coiled-coil proteins specifically located on the Golgi (termed golgins), such as p115 and GM130, have been shown to be important as tethering molecules and in the biogenesis of membranes of the Golgi stack [1–5]. A number of golgins specifically associated with the TGN (tran ...
... Members of the family of coiled-coil proteins specifically located on the Golgi (termed golgins), such as p115 and GM130, have been shown to be important as tethering molecules and in the biogenesis of membranes of the Golgi stack [1–5]. A number of golgins specifically associated with the TGN (tran ...
The Roles of Moonlighting Proteins in Bacteria
... along with their classic neuropeptide function (Campbell and Scanes, 1995). The term “moonlighting protein” was introduced to describe a single protein that has multiple functions not due to gene fusions, splice variants or multiple proteolytic fragments (Jeffery, 2009; Huberts and van der Klei, 201 ...
... along with their classic neuropeptide function (Campbell and Scanes, 1995). The term “moonlighting protein” was introduced to describe a single protein that has multiple functions not due to gene fusions, splice variants or multiple proteolytic fragments (Jeffery, 2009; Huberts and van der Klei, 201 ...
The origin of the eukaryotic cell: A genomic investigation
... small ribosomal RNA and a host of proteins have identified this cell as being an extremely early-diverging eukaryote (4). The loss of about 567 proteins as one brings Giardia into the search for ESPs is caused by the absence of a mitochondrion in Giardia and to the simplification of cellular structu ...
... small ribosomal RNA and a host of proteins have identified this cell as being an extremely early-diverging eukaryote (4). The loss of about 567 proteins as one brings Giardia into the search for ESPs is caused by the absence of a mitochondrion in Giardia and to the simplification of cellular structu ...
Bacterial microcompartment
Bacterial Microcompartments (BMCs) are organelles consisting of a protein shell that encloses enzymes and other proteins. BMCs are typically about 40-200 nanometers in diameter and are entirely made of proteins. The shell functions like a membrane, as it is selectively permeable. Other protein-based compartments found in bacteria and archaea include encapsulin nanocompartments and gas vesicles. Eukaryotes have also been observed to have proteinaceous organelles, such as the mysterious vault complex.