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Matrix Specific Protocol
Matrix Specific Protocol

... guidelines described in this technical note are optimized for manually shaking of the tubes with 2 metal balls. If using a mechanical shaker, the strength of the tubes and the number of metal balls must be considered. Tubes can easily break. Other labs using such mechanical methods recommend using o ...
Lyophilization, Concentration or Evaporation
Lyophilization, Concentration or Evaporation

... dryer with a collector of -15° C to -20° C or lower is required. If the sample is Acetonitrile, a -84˚C cascade freeze dryer is recommended. If the sample contains alcohols a -105˚C freeze dryer is needed. During primary drying, 92-93% of the moisture is removed from the sample. Labconco’s FreeZone® ...
Dried blood spot analysis on the Biochrom 30 Amino Acid Analyser
Dried blood spot analysis on the Biochrom 30 Amino Acid Analyser

... Typically the Biochrom 30 Amino Acid Analyser is used for the amino acid analysis of plasma and urine. However it can also be used for analysis of other types of samples such as dried blood spots following suitable sample preparation. Sample preparation Trichloroacetic acid (TCA) and 5-sulphosalicyl ...
When it comes to reliable automation of protein digestion for LC and
When it comes to reliable automation of protein digestion for LC and

... When it comes to reliable automation of in-gel and in-solution protein digestion followed by sample preparation for LC-MS, the choice is simple. The ProPrep LC is the only instrument that has been specifically designed for this purpose. The ProPrep LC gives you peace of mind and your samples the bes ...
7.0 Volatile Nitrogenous Bases
7.0 Volatile Nitrogenous Bases

... METHOD 7:0 Printed with effect from 1st March 2014 ...
MALDI-TOF Diagnostic Service - University of Pretoria Archived
MALDI-TOF Diagnostic Service - University of Pretoria Archived

... Upon receipt of the sample analysis request form, an analysis date will be scheduled and the researcher notified to allow sufficient time for sample preparation. There is no restriction on the number of samples to be submitted for analysis. However, samples are routinely analysed in batches of 200-2 ...
The samples were dissolved in 25 µL of 100 mM ammonium
The samples were dissolved in 25 µL of 100 mM ammonium

... Da for the major protein in the A sample and the equivalent protein in the B sample. It was also seen that there were 6 cysteines, so we postulated that there could be 3 disulfide bonds that lowers the mass by 6 Da. To determine the sequences for the other two proteins in the sample B, we looked for ...
Word - ASDL Community
Word - ASDL Community

... 1. The sample is ionized: the sample is usually ionized to a cation which is caused by the loss of an electron or addition of a proton. 2. The mass is analyzed: the ions are then separated according to their mass-to-charge ratio by an electromagnetic field. 3. The ions are detected: the separated io ...
Liggins Institute - Analytical Capabilities - Wiki
Liggins Institute - Analytical Capabilities - Wiki

... more easily. Q-Exactive High resolution quadrupole / orbitrap mass spectrometer In addition to targeted analysis via the triple quads we also have a Q-Exactive for (mainly) untargeted metabolic profiling. This machine allows researchers to see the entire ‘forest’ of compounds in a sample and compare ...
102 : Sample Pre
102 : Sample Pre

... would be fine). Sample matrix is flushed to waste. Again, it may also be desirable to wash the trap bed with a salt-free solution after loading, to ensure that any buffer salts have been rinsed away. Once the entire sample has been pumped through the trap cartridge, the elution step can commence. Pr ...
DESI - plaza
DESI - plaza

... Advantages of DESI Ambient condition Large range of molecules from small to proteins Sample can be moved in space during analysis (Imaging) No need for Matrix unlike MALDI Multiple charge ions is an advantage to extend mass range (proteins) Variable distance and angel allows different energy ...
bushmeat
bushmeat

... Sample #A62498: Sitatunga Sample #B77342: African bushed tail porcupine Sample #C79942: olive baboon Sample #C80217: African Bush Elephant Sample #C90034: Drill Sample #D51210: western gorilla Sample #G34176: Pressus's monkey Sample #G54358: greater cane rat Sample #H64991: western red colobus Sampl ...
External service charges
External service charges

... Contact details: Dr James McCullagh, CRL, Dept of Chemistry, University of Oxford, Mansfield Road, Oxford. OX1 3TA.  ...
Flow Cytometer Sample Request Form
Flow Cytometer Sample Request Form

... Is your sample TOXIC? State all biological and chemical hazards associated with your samples. _____________________________________________________________________________ Have you included all the necessary CONTROLS? _____________________________________ Please list your controls: _________________ ...
Document
Document

... Photoionzation – Multiple matrix molecule photon absorption – Energy transferred to the analyte in the solid-phase or the gas-phase ...
Amino Acid Sequencing Order Form
Amino Acid Sequencing Order Form

... Amino Acid Sequencing Order Form Requested by: ...
Mass Spectrometry Sample Analysis Form
Mass Spectrometry Sample Analysis Form

... ...
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Sample preparation in mass spectrometry

The sample preparation for mass spectrometry is used for the optimisation of a sample for the analysis in a mass spectrometer (MS).These have depending on their ion source different requirements for volume, concentration, and composition of the analyte solution. Furthermore, in protein mass spectrometry usually the protein of interest is cleaved into peptides before analysis, either by in-gel digestion or by proteolysis in solution.
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