Lecture Slides
... of male smoking is relatively high Women’s lung cancer is different from men’s lung cancer. High proportion of female lung cancer is adenocarcinoma of the lung The RR for women’s lung cancer is higher than male lung cancer, which indicates that women may be susceptible to low dose tobacco smoking ...
... of male smoking is relatively high Women’s lung cancer is different from men’s lung cancer. High proportion of female lung cancer is adenocarcinoma of the lung The RR for women’s lung cancer is higher than male lung cancer, which indicates that women may be susceptible to low dose tobacco smoking ...
Human Vascular Endothelial Growth Factor Receptor 2
... This assay has been correlated to the Quantikine® ELISA Kit with a slope of 0.9-1.1 and an R2 value greater than 0.9. ...
... This assay has been correlated to the Quantikine® ELISA Kit with a slope of 0.9-1.1 and an R2 value greater than 0.9. ...
Appendix A: Matrix primary transpositions on PIN`s adjacent
... Appendix S1: Matrix primary transpositions on PIN’s adjacent matrix keep information synchronization. The information of protein interactions in a PIN contained in the matrix is still complete after rearranging the rows and columns in the same way, and the information of the rearrangement will be sa ...
... Appendix S1: Matrix primary transpositions on PIN’s adjacent matrix keep information synchronization. The information of protein interactions in a PIN contained in the matrix is still complete after rearranging the rows and columns in the same way, and the information of the rearrangement will be sa ...
animal science nutrition laboratory standard procedures and safety
... nitrogen and crude protein content can be calculated. An example of this calculation is shown in Table 3-4. Generally, samples to be analyzed for kjeldahl nitrogen are weighed and wrapped in filter paper and then placed in the reaction vessel. Containing the sample in filter paper is simply a method ...
... nitrogen and crude protein content can be calculated. An example of this calculation is shown in Table 3-4. Generally, samples to be analyzed for kjeldahl nitrogen are weighed and wrapped in filter paper and then placed in the reaction vessel. Containing the sample in filter paper is simply a method ...
Supplementary Methods Quantitative mass spectrometry
... Briefly, peptides (4 µl) were separated by reverse phase chromatography-nLC (Acclaim PepMap RSLC, 1.8 μm x 75 μm x 50 cm) at a flow rate of 250 nL/min over a 90 min ACN gradient from 4 – 40% mobile phase B (A, 0.1% FA; B, 97% ACN in 0.1% FA) and ionized by ESI directly into the mass spectrometer (L ...
... Briefly, peptides (4 µl) were separated by reverse phase chromatography-nLC (Acclaim PepMap RSLC, 1.8 μm x 75 μm x 50 cm) at a flow rate of 250 nL/min over a 90 min ACN gradient from 4 – 40% mobile phase B (A, 0.1% FA; B, 97% ACN in 0.1% FA) and ionized by ESI directly into the mass spectrometer (L ...
Sample Preparation Guidelines for 2
... that interfere with the labeling of the protein sample with CyDye Fluor minimal dyes. These reagents include thiols used as reducing agents and polyamines used as IPG buffers (ampholytes). Samples must also be free of ionic contaminants that hamper effective isoelectric focusing of the proteins duri ...
... that interfere with the labeling of the protein sample with CyDye Fluor minimal dyes. These reagents include thiols used as reducing agents and polyamines used as IPG buffers (ampholytes). Samples must also be free of ionic contaminants that hamper effective isoelectric focusing of the proteins duri ...
TOPIC 6: CARBOHYDRATE ANALYSIS
... • Low molecular weight CHO are developed by attaching the CHO of interest to a protein and then injecting it into an animal • Antibodies specific to CHO molecule is developed then and can be extracted for determining the specific CHO concentration. • Advantages: extremely sensitive, specific, easy t ...
... • Low molecular weight CHO are developed by attaching the CHO of interest to a protein and then injecting it into an animal • Antibodies specific to CHO molecule is developed then and can be extracted for determining the specific CHO concentration. • Advantages: extremely sensitive, specific, easy t ...
ITC - University of Victoria
... calorimeters became available. Evolved from a specialist method to a widely used technique ...
... calorimeters became available. Evolved from a specialist method to a widely used technique ...
Phosphatase Assay
... The volume of sample required for the assay depends on the amount of enzyme present in the solution being evaluated. This can be estimated in the following way. First, assume that the total volume of the sample assayed is 100 µL. This means that all other components add up to a total volume of 2.900 ...
... The volume of sample required for the assay depends on the amount of enzyme present in the solution being evaluated. This can be estimated in the following way. First, assume that the total volume of the sample assayed is 100 µL. This means that all other components add up to a total volume of 2.900 ...
Choosing the metabolomics platform
... – Not all compounds can be analyzed by GC‐MS – Although amino acids, sugars, fatty acids, amines and organic acids can be derivatized, complex polyphenol glycosides and polar lipids are too unstable, even when derivatized, at the temperatures used to elute them – Approximate mass limit of 400 Da ...
... – Not all compounds can be analyzed by GC‐MS – Although amino acids, sugars, fatty acids, amines and organic acids can be derivatized, complex polyphenol glycosides and polar lipids are too unstable, even when derivatized, at the temperatures used to elute them – Approximate mass limit of 400 Da ...
Laemmli Buffer Recipe Preparation
... Purpose of the Laemmli buffer The beta 2-mercaptoethanol reduces intra and inter-molecular disulfide bonds of the proteins to allow proper separation not by shape but by size. The SDS detergent binds to all the proteins positive charges which occur at a regular interval, thus giving each protein the ...
... Purpose of the Laemmli buffer The beta 2-mercaptoethanol reduces intra and inter-molecular disulfide bonds of the proteins to allow proper separation not by shape but by size. The SDS detergent binds to all the proteins positive charges which occur at a regular interval, thus giving each protein the ...
SDS-PAGE Sample Loading Buffer - G
... Add an equal volume of SDS-PAGE Sample Loading Buffer [2X] to the tube containing protein solution. For reducing gels, add reducing agent to a final concentration of 2-5% β-mercaptoethanol or 5-20mM DTT. Vortex the tube to mix the contents. Place the sample tube in a boiling water bath for 5-10 minu ...
... Add an equal volume of SDS-PAGE Sample Loading Buffer [2X] to the tube containing protein solution. For reducing gels, add reducing agent to a final concentration of 2-5% β-mercaptoethanol or 5-20mM DTT. Vortex the tube to mix the contents. Place the sample tube in a boiling water bath for 5-10 minu ...
Mass Spectrometry
... Why MALDI? -Less sensitive to salts -Lower PRACTICAL detection limits -Easier to interpret spectra(less multiple charges) -Quick and easy -Higher mass detection -Higher Throughput(1000>samples per hour) ...
... Why MALDI? -Less sensitive to salts -Lower PRACTICAL detection limits -Easier to interpret spectra(less multiple charges) -Quick and easy -Higher mass detection -Higher Throughput(1000>samples per hour) ...
Expressing 15N labeled protein
... (i.e. 10 mL of 20% arabinose for BAI cells) Purification: As normal, however protein needs to be at a final concentration of ~0.5 – 1 mM in 25 mM phosphate buffer, pH 6.5 or lower. 0.1% sodium azide may be added to inhibit bacterial and fungal growth. A reference compound will be added to the sample ...
... (i.e. 10 mL of 20% arabinose for BAI cells) Purification: As normal, however protein needs to be at a final concentration of ~0.5 – 1 mM in 25 mM phosphate buffer, pH 6.5 or lower. 0.1% sodium azide may be added to inhibit bacterial and fungal growth. A reference compound will be added to the sample ...
Mass spectrometric characterization of a conformational epitope of
... 3Boehringer Ingelheim Pharma GmbH, ZNS Research Dept., Biberach, Germany. ...
... 3Boehringer Ingelheim Pharma GmbH, ZNS Research Dept., Biberach, Germany. ...
REQUEST FOR DNA TEST FOR TNS
... DNA will be extracted from the samples and stored at UNSW. The major diseases under study are ceroid lipofuscinosis (CL), collie eye anomaly (CEA) and Trapped Neutrophil Syndrome (TNS). The samples may be used in the study of other diseases if the research is extended in the future unless specific i ...
... DNA will be extracted from the samples and stored at UNSW. The major diseases under study are ceroid lipofuscinosis (CL), collie eye anomaly (CEA) and Trapped Neutrophil Syndrome (TNS). The samples may be used in the study of other diseases if the research is extended in the future unless specific i ...
Pochonia chlamydosporia - Biological Engineering
... the fungal polyketides, is produced by Pochonia chlamydosporia. It inhibits the Hsp90 molecular chaperone, another important target for cancer chemotherapy. Recently, gene clusters for biosynthesis of radicicol from Pochonia chlamydosporia were sequenced. However, the function of each enzyme is stil ...
... the fungal polyketides, is produced by Pochonia chlamydosporia. It inhibits the Hsp90 molecular chaperone, another important target for cancer chemotherapy. Recently, gene clusters for biosynthesis of radicicol from Pochonia chlamydosporia were sequenced. However, the function of each enzyme is stil ...
The Future of Biosensors Professor Brian Birch LIRANS University of Luton UK
... Direct immersion ...
... Direct immersion ...
Sample Preparation for Peptides/Proteins
... compounds less than ~3500 Da in mass, THAP works best and for masses higher than that, HPA is the matrix of choice. Some groups still use DHB for smaller oligonucleotides, but the most commonly used matrix for this class of compound is THAP. You will find the protocols for sample preparation for oli ...
... compounds less than ~3500 Da in mass, THAP works best and for masses higher than that, HPA is the matrix of choice. Some groups still use DHB for smaller oligonucleotides, but the most commonly used matrix for this class of compound is THAP. You will find the protocols for sample preparation for oli ...
S3 Fig - PLoS ONE
... (UC=uncut) or presence (C=cut) of Tobacco Etch Virus (TEV) protease. Equal volumes of sample were loaded onto the gel after overnight incubation and centrifugation. The fusion protein is only stable when not dialyzed, or dialyzed against 25 mM Tris pH 8.0, 5 mM MgCl2, 100 mM NaCl. The mechanism behi ...
... (UC=uncut) or presence (C=cut) of Tobacco Etch Virus (TEV) protease. Equal volumes of sample were loaded onto the gel after overnight incubation and centrifugation. The fusion protein is only stable when not dialyzed, or dialyzed against 25 mM Tris pH 8.0, 5 mM MgCl2, 100 mM NaCl. The mechanism behi ...