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Homology between the DNA-binding domain of the GCN4
Homology between the DNA-binding domain of the GCN4

... domains of the protein: DNA binding and transcriptional activation (13). The DNA-binding function depends on the integrity of the carboxyl-terminal segment of 60 amino acids (13). This highly basic region is also the domain showing homology to jun protein. Transcriptional activation by GCN4 requires ...
locating domains
locating domains

... indicate where proteins can be divided into domains. • Secondary structure prediction methods will often predict regions of proteins to have different protein structural classes. For example, one region of a sequence may be predicted to contain only a helices and another to contain only b sheets. Th ...
Desferrioxamine-mediated Iron Uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake.
Desferrioxamine-mediated Iron Uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake.

... molecular weight compounds that specifically bind ferric iron. Siderophores bind ferric iron with high affinity, and the siderophore-iron complex can be captured by specific cellular transport systems. Most bacteria and fungi synthesize at least one type of siderophore but may express transporters s ...
01. Amino Acids
01. Amino Acids

... In kinetic resolution, two enantiomers show different reaction rates in a chemical reaction, thereby creating an excess of the less reactive enantiomer. This excess goes through a maximum and disappears on full completion of the reaction. Kinetic resolution is a very old concept in organic chemistry ...
1X Equilibration/Wash Buffer (pH 7.0)
1X Equilibration/Wash Buffer (pH 7.0)

... 5. Resuspend the pellet in 2 ml of 1X Equilibration/Wash Buffer (pH 7.0) per 20–25 ml of 6. Gently agitate or stir the sample until it becomes translucent. ...
F1: Multiple alignment and its meaning
F1: Multiple alignment and its meaning

... Active sites (i.e. binding sites) are hard to mutate successfully (why?) Structure-defining amino acids are hard to change successfully (why?) ...
U4L26 Nitrogen - The University of Sydney
U4L26 Nitrogen - The University of Sydney

... – Oxidative deamination of glutamate • Regenerates 2-oxoglutarate • Releases ammonia ...
Biochemistry
Biochemistry

... (4) The code is nearly universal. The same codon specifies the same amino acid in almost all species studied; however , some differences have been found in the codons used in mitochondria. Lastly, the Genetic Code in the table above has also been called "The Universal Genetic Code". It is known as " ...
Lecture 0 - Introduction  - LCQB
Lecture 0 - Introduction - LCQB

... Glycine can adopt a much wider range of conformations than the other residues and thus plays a structurally very important role ...
Structural Bioinformatics - LCQB
Structural Bioinformatics - LCQB

... Glycine can adopt a much wider range of conformations than the other residues and thus plays a structurally very important role ...
Document
Document

... •Enzymes - proteins that catalyze chemical and biochemical reactions within living cell and outside. This group of proteins probably is the biggest and most important group of the proteins. •Hormones - proteins that are responsible for the regulation of many processes in organisms. Hormones are usu ...
World Journal of Microbiology and Biotechnology
World Journal of Microbiology and Biotechnology

... thread (although possibly less frequently than wild type cells), and form (some) bacteroid-filled V. hirsuta plant cells (De Maagd et al. 1988, 1989b; Priefer I989). In all these cases the nodules are small, white and devoid of nitrogenase activity. Thus, it appears that complete LPS is not essentia ...
Characterization of AtAAP1 function in amino acid uptake by the root
Characterization of AtAAP1 function in amino acid uptake by the root

... acid-supplied cultures, which would have lowered 13C labeling in cultures compared to that expected from the hypothesized protein content of the hyphae. We did see indirect evidence for gluconeogenesis from amino acids, in that the labeling levels when supplied with 13Clabeled amino acids were highe ...
Macromolecules
Macromolecules

... Protein Folding Within the Cell • Proteins called chaperonins assist in proper folding of newly synthesized proteins • Some chaperonins “trap” proteins in a molecular cage to prevent interference in the ...
lecture08_08
lecture08_08

... • Most alpha helices are ~12 residues long Most beta strands are ~6 residues long  Look at all windows of size 6/12  Calculate a score for each window. If >threshold  predict this is an alpha helix/beta sheet ...
SUPPLEMENTAL DATA FOR DUPLICATED SACCHAROMYCES
SUPPLEMENTAL DATA FOR DUPLICATED SACCHAROMYCES

... - G121D: Asp side chain is possibly too far to form a hydrogen bond to FMN. S3.3. Cellular localization There are conflicting reports for the location of PST2 and RFS1. In earlier studies, the green fluorescent protein (GFP)-fusion protein of PST2 localized to the cytoplasm in a punctuate pattern [5 ...
Transcript
Transcript

... Individual polypeptide chains fold into a tertiary structure and a bunch of secondary structures based on its primary structure. a. It can form a complex when it interacts with other subunits or other polypeptide chains that are not covalently linked or not coded on same gene. Either between two dif ...
chapter 3
chapter 3

... isomer form, whereas the amino acids in a large number of the plants they fed upon were in the D isomer form? 4. Many types of proteins can be isolated only in quantities that are too small for the direct determination of a primary amino acid sequence. Recent advances in gene cloning and amplificati ...
Enzymes..
Enzymes..

... E. Quantity of enzyme is not consumed during the enzymatic reaction. Find the differences between enzymes and inorganic catalysts A. High specificity B. They catalyze only energetically possible reactions C. They do not vary a reaction direction D. They accelerate reaction equilibrium beginning, but ...
Amino Acid Sequence and Antigenicity of the Amino
Amino Acid Sequence and Antigenicity of the Amino

... 168 kDa membrane protein (Hu et al., 1982; Feldner et al., 1982). Besides its function as an adhesin the 168 kDa protein is a major immunogen of M . pneumoniae. In early stages of human infection it is so far the only protein antigen known against which antibodies are developed by all patients (Leit ...
Characterization of a AT-Bromoacetyl-L-Thyroxine Affinity
Characterization of a AT-Bromoacetyl-L-Thyroxine Affinity

... increases the degradation of this short-lived membranebound protein in cultured glial cells by dynamically regulating the polymerization of the actin cytoskeleton through an energy-dependent mechanism that does not require protein synthesis or the nuclear T 3 receptor (2). This extranuclear action o ...
Hydrodynamic Studies on the Manganese
Hydrodynamic Studies on the Manganese

... In higher plants, three extrinsic proteins, with apparent molecular masses of 33, 24, and 17 kDa, are required for high rates of oxygen evolution at physiological inorganic cofactor concentrations. In cyanobacteria, only the 33 kDa component is present with the functions of the 23 and 17 kDa protein ...
bioc-2200-a-biol-2200-a-mock-final-exam
bioc-2200-a-biol-2200-a-mock-final-exam

... 15. What is true about cholesterol? a. is a steroid precursor b. interacts only with hydrophobic tails of phospholipids c. always increases membrane fluidity d. cholesterol is just horrible for you 16. Which is true about glycerophospholipids? a. they can sometimes be sphingolipids, depending on the ...
A Distinct and Parallel Pathway for the Nuclear Import of an mRNA
A Distinct and Parallel Pathway for the Nuclear Import of an mRNA

... and one member, human Crm1, was identified via its interaction with a repeat-containing nucleoporin (Fornerod et al., 1997a; Görlich et al., 1997; Wolff et al., 1997). Homology to known members of the b karyopherin family suggested that these proteins may function as karyopherins, raising the possib ...
Use of infrared and visible light radiation as modulator of protein
Use of infrared and visible light radiation as modulator of protein

... particular proteins and their target at a distance [10,11]. Thus protein interactions can be viewed as a resonant energy transfer between the interacting molecules. This energy can be transferred through oscillations of a physical field, possibly electromagnetic in nature [1]. Since there is eviden ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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