Gene Section PRUNE (prune exopolyphosphatase) Atlas of Genetics and Cytogenetics
... be divided into two main groups on the basis of a Cterminal motif that is very well conserved within each group, but not across the groups. All the members of this super-family possess four other motifs that contain highly conserved charged residues predicted to be responsible for binding ions and c ...
... be divided into two main groups on the basis of a Cterminal motif that is very well conserved within each group, but not across the groups. All the members of this super-family possess four other motifs that contain highly conserved charged residues predicted to be responsible for binding ions and c ...
1 a dictyostelium mutant with reduced lysozyme levels compensates
... alyA gene, was knocked out by homologous recombination. The mutant cells had greatly reduced enzymatic activity and grew inefficiently when bacteria were the sole food source. Over time, the mutant gained the ability to internalize bacteria more efficiently, so that the defect in digestion was compe ...
... alyA gene, was knocked out by homologous recombination. The mutant cells had greatly reduced enzymatic activity and grew inefficiently when bacteria were the sole food source. Over time, the mutant gained the ability to internalize bacteria more efficiently, so that the defect in digestion was compe ...
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... C02H-terminal parts can be incorporated into this membrane in vivo [Bremer et al. (1982) Eur. J. Biochem. 222, 223 - 2311. The possibility that these fragments can be used, via gene fusions, as vehicles to transport other proteins to the outer membrane has been investigated. To test whether fragment ...
... C02H-terminal parts can be incorporated into this membrane in vivo [Bremer et al. (1982) Eur. J. Biochem. 222, 223 - 2311. The possibility that these fragments can be used, via gene fusions, as vehicles to transport other proteins to the outer membrane has been investigated. To test whether fragment ...
Characterization of transport systems for cysteine, lysine, alanine
... a minimum of 30 min before transfer to similar medium containing radiolabeled AA. The strips were then incubated for 0, 30, 60, 90, and 120 min and repeatedly washed in cold 5% TCA to remove extracellular AA. Radiolabel uptake was estimated as described previously. ...
... a minimum of 30 min before transfer to similar medium containing radiolabeled AA. The strips were then incubated for 0, 30, 60, 90, and 120 min and repeatedly washed in cold 5% TCA to remove extracellular AA. Radiolabel uptake was estimated as described previously. ...
malt - CSU, Chico
... Steeping and germination The dry barley is hydrated to 4546% moisture with repeated steeping. Additives to steep water included gibberillins and calcium hydroxide. At 45% moisture, the barley has a small germ tube extending from it. The barley is then germinated in large aerated bunkers at 15°C It ...
... Steeping and germination The dry barley is hydrated to 4546% moisture with repeated steeping. Additives to steep water included gibberillins and calcium hydroxide. At 45% moisture, the barley has a small germ tube extending from it. The barley is then germinated in large aerated bunkers at 15°C It ...
High Density Cobalt Agarose
... imidazole is sufficient to elute the protein. It is also possible to apply a concentration gradient of imidazole (0 - 0.5 M). Most proteins are eluted at imidazole concentrations around 250 mM. Other reagents that can be used as competitive ligands are histidine and ammonium chloride. Please note: Us ...
... imidazole is sufficient to elute the protein. It is also possible to apply a concentration gradient of imidazole (0 - 0.5 M). Most proteins are eluted at imidazole concentrations around 250 mM. Other reagents that can be used as competitive ligands are histidine and ammonium chloride. Please note: Us ...
S100 Protein (P)
... specific application. Use TBS for washing steps. Limitations: The optimum antibody dilution and protocols for a specific application can vary. These include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue section thickness and detection kit used. Due to the superio ...
... specific application. Use TBS for washing steps. Limitations: The optimum antibody dilution and protocols for a specific application can vary. These include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue section thickness and detection kit used. Due to the superio ...
440-kD Ankyrins: Structure of the Major
... PstI; V,PvulI. Individual clones are numbered on the right. (B) Derived amino acid sequence of the 440-kD ankyrinB insert region. 2,085 amino acids were deduced from the cDNA sequence. The first and last amino acid represent the start and end of the insert region. Potential protein phosphorylation s ...
... PstI; V,PvulI. Individual clones are numbered on the right. (B) Derived amino acid sequence of the 440-kD ankyrinB insert region. 2,085 amino acids were deduced from the cDNA sequence. The first and last amino acid represent the start and end of the insert region. Potential protein phosphorylation s ...
The Dock and Lock Method: A Novel
... Plains, New Jersey ; and 3 Garden State Cancer Center, Center for Molecular Medicine and Immunology, Belleville, NewJersey ...
... Plains, New Jersey ; and 3 Garden State Cancer Center, Center for Molecular Medicine and Immunology, Belleville, NewJersey ...
The SUPERFAMILY database in structural genomics
... determination. Some approaches which may be aided by the use of the SUPERFAMILY database are described here. 3.1.1. Novel folds. There are a limited number of common protein folds in nature (Chothia, 1992), many of which have at least one representative structure solved. There are, however, many fol ...
... determination. Some approaches which may be aided by the use of the SUPERFAMILY database are described here. 3.1.1. Novel folds. There are a limited number of common protein folds in nature (Chothia, 1992), many of which have at least one representative structure solved. There are, however, many fol ...
Comparative Analysis of Two C-Terminal Kinesin Motor
... Xenopus XCTK2 is required for the formation of wellfocused asters and bipolar spindles in Xenopus egg extracts [Walczak et al., 1997]. HSET is required, in combination with NuMA, for the formation of asters in cell-free systems and chromosomal movement in cultured cells [Gaglio et al., 1997; Gordon ...
... Xenopus XCTK2 is required for the formation of wellfocused asters and bipolar spindles in Xenopus egg extracts [Walczak et al., 1997]. HSET is required, in combination with NuMA, for the formation of asters in cell-free systems and chromosomal movement in cultured cells [Gaglio et al., 1997; Gordon ...
Role of Bioinformatics Tools in Biological Research GPS
... probable intron and exon regions are compared to filter/remove wrong exons; NNSPLICE program is used to reassign splicing signal site positions ...
... probable intron and exon regions are compared to filter/remove wrong exons; NNSPLICE program is used to reassign splicing signal site positions ...
Bis2A 09.0 Membranes: Components and Structure
... The mosaic characteristic of the membrane, described in the uid mosaic model, helps to illustrate its nature. The integral proteins and lipids exist in the membrane as separate but loosely attached molecules. These resemble the separate, multicolored tiles of a mosaic picture, and they oat, moving ...
... The mosaic characteristic of the membrane, described in the uid mosaic model, helps to illustrate its nature. The integral proteins and lipids exist in the membrane as separate but loosely attached molecules. These resemble the separate, multicolored tiles of a mosaic picture, and they oat, moving ...
Chimeric phosphorylation indicator
... munoprecipitation assays, and tWo-hybrid screens require destroying large numbers of the cells or transferring genes to heterologous organisms. As such, these methods have poor temporal and spatial resolution, and are insu?icient to ...
... munoprecipitation assays, and tWo-hybrid screens require destroying large numbers of the cells or transferring genes to heterologous organisms. As such, these methods have poor temporal and spatial resolution, and are insu?icient to ...
Cell signaling under salt, water and cold stresses Jian
... stress, the SOS3–SOS2 kinase complex also seems to activate the Na+/H+ antiporter activity in isolated plasma membrane vesicles (Q Qiu, K Schumaker, J-K Zhu, unpublished data). Future genetic suppressor and enhancer screens in sos mutant backgrounds, protein interaction screens with the SOS proteins ...
... stress, the SOS3–SOS2 kinase complex also seems to activate the Na+/H+ antiporter activity in isolated plasma membrane vesicles (Q Qiu, K Schumaker, J-K Zhu, unpublished data). Future genetic suppressor and enhancer screens in sos mutant backgrounds, protein interaction screens with the SOS proteins ...
Paper Title
... First approach was based on the idea of selecting a subset of properties with high orthogonality. To prove this idea, 544 properties from AAIndex were clustered by K-means algorithm [20] into {5, 6, 7, 8, 9, 10} clusters and 20,000 of experiments were proceeded on MMP dataset for each cluster count ...
... First approach was based on the idea of selecting a subset of properties with high orthogonality. To prove this idea, 544 properties from AAIndex were clustered by K-means algorithm [20] into {5, 6, 7, 8, 9, 10} clusters and 20,000 of experiments were proceeded on MMP dataset for each cluster count ...
The origin of the eukaryotic cell: A genomic investigation
... cholesterol, and steroids are synthesized in the smooth ER. The rough ER is so designated because it is a membrane decorated by ribosomes, where proteins are synthesized, folded, and packaged for transport to the Golgi apparatus. This relationship between the smooth and rough ER is indicative of an ...
... cholesterol, and steroids are synthesized in the smooth ER. The rough ER is so designated because it is a membrane decorated by ribosomes, where proteins are synthesized, folded, and packaged for transport to the Golgi apparatus. This relationship between the smooth and rough ER is indicative of an ...
Cloning and characterization of the nuclear
... The synthesis of the D2 protein requires the products of at least three nuclear genes (NAC1, AC115, and NAC2). The NAC2 gene encodes a product which is required for the stabilization of psbD mRNA [27]. Mutation of the NAC2 gene results in the complete absence of psbD mRNA [27]. To confer stability, ...
... The synthesis of the D2 protein requires the products of at least three nuclear genes (NAC1, AC115, and NAC2). The NAC2 gene encodes a product which is required for the stabilization of psbD mRNA [27]. Mutation of the NAC2 gene results in the complete absence of psbD mRNA [27]. To confer stability, ...
The Chemical Building Blocks of Life
... Carbon and hydrogen atoms both have very similar electronegativities, so electrons in C—C and C—H bonds are evenly distributed, and there are no significant differences in charge over the molecular surface. For this reason, hydrocarbons are nonpolar. Most organic molecules that are produced by cells ...
... Carbon and hydrogen atoms both have very similar electronegativities, so electrons in C—C and C—H bonds are evenly distributed, and there are no significant differences in charge over the molecular surface. For this reason, hydrocarbons are nonpolar. Most organic molecules that are produced by cells ...
How to study proteins by circular dichroism
... (see Section 6) and that the CD instrument is properly calibrated and operated (see Sections 7 and 8). Sophisticated analysis will not transform bad data into good data! 3.2. Tertiary structure fingerprint The spectra in the region 260– 320 nm arise from the aromatic amino acids. Each of the amino a ...
... (see Section 6) and that the CD instrument is properly calibrated and operated (see Sections 7 and 8). Sophisticated analysis will not transform bad data into good data! 3.2. Tertiary structure fingerprint The spectra in the region 260– 320 nm arise from the aromatic amino acids. Each of the amino a ...