Kay Hofmann - Tresch Group
... • The number of observed peptides divided by the number of observable peptides per protein. • Related to the logarithm of protein abundance ...
... • The number of observed peptides divided by the number of observable peptides per protein. • Related to the logarithm of protein abundance ...
Growth Hormone Releasing Hormone (GHRH) - Cloud
... Accurate Molecular Mass: 43.5kDa as determined by SDS-PAGE reducing conditions. Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.) Note: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice ...
... Accurate Molecular Mass: 43.5kDa as determined by SDS-PAGE reducing conditions. Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.) Note: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice ...
Mouse Direct PCR Kit
... • Protease Plus: For rapid and efficient digestion of mouse tissue in only 30 minutes! • 2 x M-PCR OPTI Mix: Includes Biotool's optimized Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer. ...
... • Protease Plus: For rapid and efficient digestion of mouse tissue in only 30 minutes! • 2 x M-PCR OPTI Mix: Includes Biotool's optimized Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer. ...
Characterization of Extracellular Protease Lactic Acid Bacteria From
... Total and specific enzyme activity increasing extracellular protease activity of crude into pure extracellular proteases. This suggests that the use of 50% ammonium sulfate (w/v) precipitation able to increasing protease activity. This is in accordance with the nature of protein will precipitate due ...
... Total and specific enzyme activity increasing extracellular protease activity of crude into pure extracellular proteases. This suggests that the use of 50% ammonium sulfate (w/v) precipitation able to increasing protease activity. This is in accordance with the nature of protein will precipitate due ...
TRYPSIN / LYS
... Specificity: Serine endopeptidase that specifically hydrolyzes proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved. Optimum pH: 8.0 Description Trypsin specifically hydrolyzes peptide bonds at the carboxylic sides of l ...
... Specificity: Serine endopeptidase that specifically hydrolyzes proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved. Optimum pH: 8.0 Description Trypsin specifically hydrolyzes peptide bonds at the carboxylic sides of l ...
Construct name
... regime of data dependent MS to MS/MS switch. A blank run ensuring lack of cross ...
... regime of data dependent MS to MS/MS switch. A blank run ensuring lack of cross ...
R. Prabhakar - Emerson Center for Scientific Computation
... Department of Chemistry, University of Miami, Coral Gables, FL 33146 Theoretical and computational techniques are used to study complex processes that are required in a wide range of biological, biotechnological and industrial applications. We have applied these techniques to investigate the mechani ...
... Department of Chemistry, University of Miami, Coral Gables, FL 33146 Theoretical and computational techniques are used to study complex processes that are required in a wide range of biological, biotechnological and industrial applications. We have applied these techniques to investigate the mechani ...
Protein Extraction Protocol
... 1. Obtain ice, and use it to chill the extraction buffer and the mortar and pestle. Clearly label two 1.5 ml microcentrifuge tubes for each plant tissue from which you want to obtain protein with the name of the plant and your groups initials. 2. From each plant cut up 1 g of fresh plant tissue as f ...
... 1. Obtain ice, and use it to chill the extraction buffer and the mortar and pestle. Clearly label two 1.5 ml microcentrifuge tubes for each plant tissue from which you want to obtain protein with the name of the plant and your groups initials. 2. From each plant cut up 1 g of fresh plant tissue as f ...
lecture 17
... AAA proteases mediate the degradation of membrane proteins in bacteria, mitochondria and chloroplasts (i.e., compartments of eubacterial origin) bacterial Lon, FtsH combine proteolytic and chaperone activities in one system, acting as quality-control machineries ...
... AAA proteases mediate the degradation of membrane proteins in bacteria, mitochondria and chloroplasts (i.e., compartments of eubacterial origin) bacterial Lon, FtsH combine proteolytic and chaperone activities in one system, acting as quality-control machineries ...
magnetic GFP-Trap -M for Immunoprecipitation of GFP
... magnetic GFP-Trap®-M for Immunoprecipitation of GFP-Fusion Proteins (gtm) For the immunoprecipitation of GFP-fusion-proteins from cellular extracts. Only for research applications, not for diagnostic or therapeutic use 1. Introduction Green fluorescent proteins (GFP) and variants thereof are widely ...
... magnetic GFP-Trap®-M for Immunoprecipitation of GFP-Fusion Proteins (gtm) For the immunoprecipitation of GFP-fusion-proteins from cellular extracts. Only for research applications, not for diagnostic or therapeutic use 1. Introduction Green fluorescent proteins (GFP) and variants thereof are widely ...
Determination of Amino Acid Sequence
... Sequencing Large Proteins Breaking disulfide bonds Oxidation by performic acid Reduction and carboxymethylation Cleaving the polypeptide chain Using proteases Cleavage of peptide bond next to particular amino acid residues ...
... Sequencing Large Proteins Breaking disulfide bonds Oxidation by performic acid Reduction and carboxymethylation Cleaving the polypeptide chain Using proteases Cleavage of peptide bond next to particular amino acid residues ...
Critical role of amino acid 23 in mediating activity - Craik Lab
... of illnesses and over one million deaths each year [1]. Treatment of malaria has relied on a small number of drugs, and the emergence of drug-resistant parasites poses a major challenge. Thus the identification and characterization of new targets for anti-malarial chemotherapy is an urgent priority. ...
... of illnesses and over one million deaths each year [1]. Treatment of malaria has relied on a small number of drugs, and the emergence of drug-resistant parasites poses a major challenge. Thus the identification and characterization of new targets for anti-malarial chemotherapy is an urgent priority. ...
Antiviral agents
... differences is far from the active site so high possibility to get inhibitors against both a the same time ...
... differences is far from the active site so high possibility to get inhibitors against both a the same time ...
Disentangling factors of gene expression regulation in human
... a cell can be regulated both at transcription and translation stage. The relative contribution of each of these steps has been a long‐standing subject of research and discussions in the literature, however the conclusions of different studies have often been contradictory, the extent of reported ...
... a cell can be regulated both at transcription and translation stage. The relative contribution of each of these steps has been a long‐standing subject of research and discussions in the literature, however the conclusions of different studies have often been contradictory, the extent of reported ...
information. - Magellan BioScience
... motifs that can be identified to have new activity. These can be discovered as new leads for drug or other research. From these leads a smaller peptide can be made or changes in the sequence can be studied to increase the activity. Considering 3 amino acids as a motif (similar to an antigenic determ ...
... motifs that can be identified to have new activity. These can be discovered as new leads for drug or other research. From these leads a smaller peptide can be made or changes in the sequence can be studied to increase the activity. Considering 3 amino acids as a motif (similar to an antigenic determ ...
Supplementary Table 1: A complete list of proteins identified with
... Supplementary Table 1: A complete list of proteins identified with two or more peptides using MaxQuant (version 1.2.2.5) from experiments using anti-acetyl-lysine immunoprecipitation and SILAC (stable isotope labeling with amino acids in cell culture) analysis of MOLM-13 cells treated with nutlin-3 ...
... Supplementary Table 1: A complete list of proteins identified with two or more peptides using MaxQuant (version 1.2.2.5) from experiments using anti-acetyl-lysine immunoprecipitation and SILAC (stable isotope labeling with amino acids in cell culture) analysis of MOLM-13 cells treated with nutlin-3 ...
Quiz-2
... Thr-Thr-Lys-Ala-gly-Tyr 9. Primary structure of cytochrome c from tissue sample of unknown species was compared to some of the known species. Based on the following results describe the evolutionary relationship of the unknown species to the known one. No of AA changed Known species ...
... Thr-Thr-Lys-Ala-gly-Tyr 9. Primary structure of cytochrome c from tissue sample of unknown species was compared to some of the known species. Based on the following results describe the evolutionary relationship of the unknown species to the known one. No of AA changed Known species ...
Mass spectrometric characterization of a conformational epitope of
... analysis of the ligand eluate [1]. ESI-MS systems from all major manufacturers can be coupled, using a newly developed software. Recent applications of the online bioaffinity- MS show broad bioanalytical potential for direct interaction studies from biological material, as diverse as antigen-antibod ...
... analysis of the ligand eluate [1]. ESI-MS systems from all major manufacturers can be coupled, using a newly developed software. Recent applications of the online bioaffinity- MS show broad bioanalytical potential for direct interaction studies from biological material, as diverse as antigen-antibod ...
TheraGest - ProThera
... down fat, carbohydrate, and protein. Digestive enzymes assist the body in breaking down large food particles into smaller particles that can be efficiently absorbed by the intestinal tract. Improper digestion can lead to abdominal discomfort such as bloating, feeling of fullness and constipation as ...
... down fat, carbohydrate, and protein. Digestive enzymes assist the body in breaking down large food particles into smaller particles that can be efficiently absorbed by the intestinal tract. Improper digestion can lead to abdominal discomfort such as bloating, feeling of fullness and constipation as ...
Mass Spectrometry-Grade Endoproteinases
... The Thermo Scientific Mass Spectrometry-Grade Endoproteinases offer specific cleavage at multiple sites (Table 1) that enable exploration of primary protein structure. Effective protein characterization and identification by mass spectrometry (MS) begins with protein digestion. Trypsin is the protea ...
... The Thermo Scientific Mass Spectrometry-Grade Endoproteinases offer specific cleavage at multiple sites (Table 1) that enable exploration of primary protein structure. Effective protein characterization and identification by mass spectrometry (MS) begins with protein digestion. Trypsin is the protea ...
XXIV Reunião Anual da FeSBE
... with activity thrombin-like from B. andianus snake venom. Methods: A new serine protease with thrombin-like activity from B. andianus snake venom (TLBan) was obtained through two chromatographic steps, molecular exclusion chromatography on G-75 column (1 cm x 60 cm) and RP-HPLC on μBondapak C ...
... with activity thrombin-like from B. andianus snake venom. Methods: A new serine protease with thrombin-like activity from B. andianus snake venom (TLBan) was obtained through two chromatographic steps, molecular exclusion chromatography on G-75 column (1 cm x 60 cm) and RP-HPLC on μBondapak C ...
Avoiding Proteolysis During Protein Chromatography.
... This temperature will not only reduce the activity of proteinases, but will also aid in stabilizing the target protein (reduction in thermal denaturation). Kulakowska-Bodzon and co-workers (24) provide an excellent review on protein preparation from various cell types for proteomic work. In general, ...
... This temperature will not only reduce the activity of proteinases, but will also aid in stabilizing the target protein (reduction in thermal denaturation). Kulakowska-Bodzon and co-workers (24) provide an excellent review on protein preparation from various cell types for proteomic work. In general, ...
When it comes to reliable automation of protein digestion for LC and
... When it comes to reliable automation of in-gel and in-solution protein digestion followed by sample preparation for LC-MS, the choice is simple. The ProPrep LC is the only instrument that has been specifically designed for this purpose. The ProPrep LC gives you peace of mind and your samples the bes ...
... When it comes to reliable automation of in-gel and in-solution protein digestion followed by sample preparation for LC-MS, the choice is simple. The ProPrep LC is the only instrument that has been specifically designed for this purpose. The ProPrep LC gives you peace of mind and your samples the bes ...