Midterm Exam Advanced Biochemistry II (Answer) 1. At equilibrium
... Suppose skeletal muscle were devoid of lactate dehydrogenase. Could it carry out strenuous physical activity; that is, could it generate ATP at a high rate by glycolysis? Explain. Answer The key point here is that NAD_ must be regenerated from NADH in order for glycolysis to continue. Some tissues, ...
... Suppose skeletal muscle were devoid of lactate dehydrogenase. Could it carry out strenuous physical activity; that is, could it generate ATP at a high rate by glycolysis? Explain. Answer The key point here is that NAD_ must be regenerated from NADH in order for glycolysis to continue. Some tissues, ...
Enzymes
... Glycolytic enzymes and their functions in the metabolic pathway of glycolysis. Several enzymes can work together in a specific order, creating metabolic pathways. In a metabolic pathway, one enzyme takes the product of another enzyme as a substrate. After the catalytic reaction, the product is then ...
... Glycolytic enzymes and their functions in the metabolic pathway of glycolysis. Several enzymes can work together in a specific order, creating metabolic pathways. In a metabolic pathway, one enzyme takes the product of another enzyme as a substrate. After the catalytic reaction, the product is then ...
Kevin Ahern's Biochemistry Course (BB 350) at Oregon State University
... two enzymes are enoyl-CoA isomerase and 2,4 dienoyl-CoA reductase (also known as Dina). The first enzyme catalyzes conversion of cis bonds between carbons 3 and 4 to trans bonds between carbons 2 and 3 so it can be oxidized in beta oxidation. Dina catalyzes conversion of two double bonds into one ci ...
... two enzymes are enoyl-CoA isomerase and 2,4 dienoyl-CoA reductase (also known as Dina). The first enzyme catalyzes conversion of cis bonds between carbons 3 and 4 to trans bonds between carbons 2 and 3 so it can be oxidized in beta oxidation. Dina catalyzes conversion of two double bonds into one ci ...
The lower Vmax is consistent with the mutation resulting in a
... In normal freshly isolated B lymphocytes and in cultured Daudi cells (an established leukemic B lymphocyte cell line used in tissue culturing) Leu-13 is absent from this complex or present at low levels. However, addition of pure recombinant Leu-13 to either fresh B lymphocytes or Daudi cells rever ...
... In normal freshly isolated B lymphocytes and in cultured Daudi cells (an established leukemic B lymphocyte cell line used in tissue culturing) Leu-13 is absent from this complex or present at low levels. However, addition of pure recombinant Leu-13 to either fresh B lymphocytes or Daudi cells rever ...
05. Clinical enzymology (1)
... more inside the RBC than in plasma, and therefore minor amount of hemolysis will result in a false-positive test. ...
... more inside the RBC than in plasma, and therefore minor amount of hemolysis will result in a false-positive test. ...
Problem set answers
... 4. AlphaCP1 is a polyC/U binding protein that binds to polyC/U-containing 3' UTR regions of certain mRNA species to stabilize the message and suppress translation. Phosphorylation of aCP1 by S6 protein kinase is thought to result in reduced binding to the mRNA and release of the protein from the po ...
... 4. AlphaCP1 is a polyC/U binding protein that binds to polyC/U-containing 3' UTR regions of certain mRNA species to stabilize the message and suppress translation. Phosphorylation of aCP1 by S6 protein kinase is thought to result in reduced binding to the mRNA and release of the protein from the po ...
Pset 1 Solutions
... An eye lens is comprised of cells that are created when an eye is formed and are retained for its lifetime. These cells lack organelles and can be regarded as “sacs” that are filled with a loose uniform arrangement of water-soluble structural proteins called crystallins. The uniform distribution of ...
... An eye lens is comprised of cells that are created when an eye is formed and are retained for its lifetime. These cells lack organelles and can be regarded as “sacs” that are filled with a loose uniform arrangement of water-soluble structural proteins called crystallins. The uniform distribution of ...
Metabol Nutri-ClinEnz Med 2_6 Nov 2012
... I. Clinical Correlations of Enzymes: Principles of Diagnostic Enzymology 1. Introduction: A human cell contains 28,602 different proteins, 2,709 proteins of which are enzymes. They are assigned roles in ~ 135 metabolic pathways (2,645 metabolites) They are distributed in: cytosol, nucleus, rough an ...
... I. Clinical Correlations of Enzymes: Principles of Diagnostic Enzymology 1. Introduction: A human cell contains 28,602 different proteins, 2,709 proteins of which are enzymes. They are assigned roles in ~ 135 metabolic pathways (2,645 metabolites) They are distributed in: cytosol, nucleus, rough an ...
Diapositive 1
... the primary sequence that define the journey of a given protein in the cell and its final localization. A single protein may contain several targeting and sorting signals. A signal sequence consists of about 20 amino acids at the N-terminal end of the primary sequence of a protein. It allows insert ...
... the primary sequence that define the journey of a given protein in the cell and its final localization. A single protein may contain several targeting and sorting signals. A signal sequence consists of about 20 amino acids at the N-terminal end of the primary sequence of a protein. It allows insert ...
Enzymes
... Regulation of Enzyme Synthesis The four mechanisms described above regulate the activity of enzymes already present within the cell. What about enzymes that are not needed or are needed but not present? Here, too, control mechanisms are at work that regulate the rate at which new enzymes are synthes ...
... Regulation of Enzyme Synthesis The four mechanisms described above regulate the activity of enzymes already present within the cell. What about enzymes that are not needed or are needed but not present? Here, too, control mechanisms are at work that regulate the rate at which new enzymes are synthes ...
Technical Information - BIOLOG Life Science Institute
... Kirchberger, T.;Wagner, G.; Xu, J.; Cordiglieri, C.; Wang, P.; Gasser, A.; Fliegert, R.; Bruhn, S.; Flügel, A.; Lund, F.E.; Zhang, L.-h.; Potter, B.V.L.; Guse, A.H., Br. J. Pharmacol., 149, 337 - 344 (2006): "Cellular Effects and Metabolic Stability of N1-cyclic Inosine Diphosphoribose and its Deri ...
... Kirchberger, T.;Wagner, G.; Xu, J.; Cordiglieri, C.; Wang, P.; Gasser, A.; Fliegert, R.; Bruhn, S.; Flügel, A.; Lund, F.E.; Zhang, L.-h.; Potter, B.V.L.; Guse, A.H., Br. J. Pharmacol., 149, 337 - 344 (2006): "Cellular Effects and Metabolic Stability of N1-cyclic Inosine Diphosphoribose and its Deri ...
4. Organic Cmpd
... There are several types of lipids, but all contain subunits of glycerol and fatty acids made of carbon, hydrogen, and oxygen. It is different from a carbohydrate because of the ratio and because the smaller units do not link together to form a chemical chain ...
... There are several types of lipids, but all contain subunits of glycerol and fatty acids made of carbon, hydrogen, and oxygen. It is different from a carbohydrate because of the ratio and because the smaller units do not link together to form a chemical chain ...
Molecular Interactions in Cell events
... This changes the trypsinogen into the active form trypsin Trypsin then helps to activate more trypsinogen molecules ...
... This changes the trypsinogen into the active form trypsin Trypsin then helps to activate more trypsinogen molecules ...
allosteric activator
... the organism cope with changing conditions and stress in its environment. ...
... the organism cope with changing conditions and stress in its environment. ...
Chapter 7 - Coenzymes
... 1) retinol and 2) retinoic acid - binds to intracellular protein receptors --> regulates gene expression 2) retinal - prosthetic group of rhodopsin Vitamin D Exists as several lipids; 1) D3 - made in skin exposed to sunlight. 2) D2 - additive in fortified milk Deficiency causes rickets in children o ...
... 1) retinol and 2) retinoic acid - binds to intracellular protein receptors --> regulates gene expression 2) retinal - prosthetic group of rhodopsin Vitamin D Exists as several lipids; 1) D3 - made in skin exposed to sunlight. 2) D2 - additive in fortified milk Deficiency causes rickets in children o ...
Executive Stress Formula
... for enzyme supplementation. Developed by leading scientists and used by millions of people worldwide, Wobenzym N has proven to be a safe and useful product. In the human body, hundreds of chemical reactions occur during the course of normal metabolic processes. Many chemical reactions require signif ...
... for enzyme supplementation. Developed by leading scientists and used by millions of people worldwide, Wobenzym N has proven to be a safe and useful product. In the human body, hundreds of chemical reactions occur during the course of normal metabolic processes. Many chemical reactions require signif ...
Sol. RUBISC - askIITians
... Some chemicals prevent the enzyme to function, are known as inhibitors. Enzymatic competitive inhibition is done by the substrate which very closely resembles the substrate in its molecular structure. Enzyme + Inhibitor =Enzyme inhibitor complex.Eg.malonate inhibits the action of succinate dehydroge ...
... Some chemicals prevent the enzyme to function, are known as inhibitors. Enzymatic competitive inhibition is done by the substrate which very closely resembles the substrate in its molecular structure. Enzyme + Inhibitor =Enzyme inhibitor complex.Eg.malonate inhibits the action of succinate dehydroge ...
The metabolic advantage of tumor cells Open Access Maurice Israël
... blockade also limits the supply of fumarate to the Krebs cycle. The latter, utilizes the a ketoglutarate provided by the transaminase reaction, since a ketoglutarate coming via aconitase slows down. Indeed, NO and peroxynitrite increase in tumors and probably block aconitase. The Figure 1 indicates ...
... blockade also limits the supply of fumarate to the Krebs cycle. The latter, utilizes the a ketoglutarate provided by the transaminase reaction, since a ketoglutarate coming via aconitase slows down. Indeed, NO and peroxynitrite increase in tumors and probably block aconitase. The Figure 1 indicates ...
Integrity and purity of the mitochondrial fraction
... positive for MitoTracker staining (Fig. S1A). The mitochondrial fraction was also essentially free of cytoplasmic contaminants such as GAPDH (absent by western blot, Fig. S1B), and the cytosolic enzyme lactate dehydrogenase. The activity of the latter enzyme accounted for < 20% of that found in cyto ...
... positive for MitoTracker staining (Fig. S1A). The mitochondrial fraction was also essentially free of cytoplasmic contaminants such as GAPDH (absent by western blot, Fig. S1B), and the cytosolic enzyme lactate dehydrogenase. The activity of the latter enzyme accounted for < 20% of that found in cyto ...
EXAM 2 Lecture 15 1. What are cofactors? A: They are small organic
... 5. What does it mean to be loosely bound? What are two examples he provided? A: molecule or ion will bind to the enzyme, carry out reaction, then dissociate from the enzyme; ATP and Mg2+ 6. What does it mean to be tightly bound? What are five examples he provided? A: may be covalently attached to an ...
... 5. What does it mean to be loosely bound? What are two examples he provided? A: molecule or ion will bind to the enzyme, carry out reaction, then dissociate from the enzyme; ATP and Mg2+ 6. What does it mean to be tightly bound? What are five examples he provided? A: may be covalently attached to an ...
How to build a glycinergic postsynaptic ...
... a 93x103Mr GlyR-associated protein, is proposed to be crucial for anchoring GlyRs at postsynaptic membranes by interaction with subsynaptic tubulin. Consistent with this view, the presence of ‘membrane-bound’ tubulin in brain synaptosomes and postsynaptic densities has been reported by several inves ...
... a 93x103Mr GlyR-associated protein, is proposed to be crucial for anchoring GlyRs at postsynaptic membranes by interaction with subsynaptic tubulin. Consistent with this view, the presence of ‘membrane-bound’ tubulin in brain synaptosomes and postsynaptic densities has been reported by several inves ...
bioc-2200-a-biol-2200-a-mock-final-exam
... 15. What is true about cholesterol? a. is a steroid precursor b. interacts only with hydrophobic tails of phospholipids c. always increases membrane fluidity d. cholesterol is just horrible for you 16. Which is true about glycerophospholipids? a. they can sometimes be sphingolipids, depending on the ...
... 15. What is true about cholesterol? a. is a steroid precursor b. interacts only with hydrophobic tails of phospholipids c. always increases membrane fluidity d. cholesterol is just horrible for you 16. Which is true about glycerophospholipids? a. they can sometimes be sphingolipids, depending on the ...
Ch.24Pt.6_000
... sequence of cycles needed to produce a C16 fatty acid from acetyl ACP. Malonyl ACP adds 2 carbons at each cycle. ...
... sequence of cycles needed to produce a C16 fatty acid from acetyl ACP. Malonyl ACP adds 2 carbons at each cycle. ...
Micronutrient Cofactors
... which DP ribosylation of EF-2 which blocks protein synthesis in host cell Cholera toxin and Pertussis toxin modify a specific Gprotein with ADP ribose which leads to deregulated protein kinase A Vitamin B3 deficiency causes pellagra 4 Ds: diarrhea, dermatitis, dementia, and death relationship betwee ...
... which DP ribosylation of EF-2 which blocks protein synthesis in host cell Cholera toxin and Pertussis toxin modify a specific Gprotein with ADP ribose which leads to deregulated protein kinase A Vitamin B3 deficiency causes pellagra 4 Ds: diarrhea, dermatitis, dementia, and death relationship betwee ...
Lipid signaling
Lipid signaling, broadly defined, refers to any biological signaling event involving a lipid messenger that binds a protein target, such as a receptor, kinase or phosphatase, which in turn mediate the effects of these lipids on specific cellular responses. Lipid signaling is thought to be qualitatively different from other classical signaling paradigms (such as monoamine neurotransmission) because lipids can freely diffuse through membranes (see osmosis.) One consequence of this is that lipid messengers cannot be stored in vesicles prior to release and so are often biosynthesized ""on demand"" at their intended site of action. As such, many lipid signaling molecules cannot circulate freely in solution but, rather, exist bound to special carrier proteins in serum.