Fritz Lipmann - Nobel Lecture
... there towards a general concept of transfer of activated groupings by carrier as the fundamental reaction in biosynthesis8,9. Although in the related manner the appearance of acetyl phosphate as a metabolic intermediary first focussed attention to possible mechanisms for the metabolic elaboration of ...
... there towards a general concept of transfer of activated groupings by carrier as the fundamental reaction in biosynthesis8,9. Although in the related manner the appearance of acetyl phosphate as a metabolic intermediary first focussed attention to possible mechanisms for the metabolic elaboration of ...
25. biosynthesis of lipids
... This reaction is very similar to other biotin-dependent carboxylation reactions, such as those catalyzed by pyruvate carboxylase and propionyl-CoA carboxylase. Acetyl-CoA carboxylase is also important because it is a regulatory step ; citrate acts as an allosteric activator for the animal enzyme, bu ...
... This reaction is very similar to other biotin-dependent carboxylation reactions, such as those catalyzed by pyruvate carboxylase and propionyl-CoA carboxylase. Acetyl-CoA carboxylase is also important because it is a regulatory step ; citrate acts as an allosteric activator for the animal enzyme, bu ...
Saccharomyces cerevisiae Metabolic engineering of for production
... importance in industrial biotechnology, it is increasingly being complemented by metabolic engineering, which has been defined as ‘the improvement of cellular activities by manipulation of enzymatic, transport, and regulatory functions of the cell with the use of recombinant DNA technology’ (Bailey, ...
... importance in industrial biotechnology, it is increasingly being complemented by metabolic engineering, which has been defined as ‘the improvement of cellular activities by manipulation of enzymatic, transport, and regulatory functions of the cell with the use of recombinant DNA technology’ (Bailey, ...
Improvement of poly-γ-glutamic acid (PGA)
... provided the maximum PGA production and growth steadily in L-glutamic acid supplementsubmerged fermentation at 37oC during 12-72 h. The characteristics of genomic DNA band of the five mutant isolates by random primer (5’-AGTCAGCCAC-3’) with a random amplified polymorphic DNA (RAPD) technique were si ...
... provided the maximum PGA production and growth steadily in L-glutamic acid supplementsubmerged fermentation at 37oC during 12-72 h. The characteristics of genomic DNA band of the five mutant isolates by random primer (5’-AGTCAGCCAC-3’) with a random amplified polymorphic DNA (RAPD) technique were si ...
Mitochondrial fatty acid oxidation alterations in heart failure
... heart disease and diabetic cardiomyopathies, changes in cardiac mitochondrial energy metabolism contribute to contractile dysfunction and to a decrease in cardiac efficiency. Specific metabolic changes include a relative increase in cardiac fatty acid oxidation rates and an uncoupling of glycolysis ...
... heart disease and diabetic cardiomyopathies, changes in cardiac mitochondrial energy metabolism contribute to contractile dysfunction and to a decrease in cardiac efficiency. Specific metabolic changes include a relative increase in cardiac fatty acid oxidation rates and an uncoupling of glycolysis ...
Original papers QJM
... Large neutral amino acids circulating in blood plasma are precursors for the synthesis in brain of the monoamines, as well as other putative neurotransmitters such as carnosine, and important cofactors such as S-adenosyl-methionine,18 and alterations in their concentrations or turnover in the brain ...
... Large neutral amino acids circulating in blood plasma are precursors for the synthesis in brain of the monoamines, as well as other putative neurotransmitters such as carnosine, and important cofactors such as S-adenosyl-methionine,18 and alterations in their concentrations or turnover in the brain ...
TQ bank Lab 4
... Over 90% of streptococcal infections are caused by _________________. A. Streptococcus B. Neisseria C. Beta-hemolytic group A streptococci D. Haemophilus Blood agar is usually made with defibrinated sheep blood, ______________ and nutrient agar. A. Sodium chloride B. Pig blood C. Protein D. Potassiu ...
... Over 90% of streptococcal infections are caused by _________________. A. Streptococcus B. Neisseria C. Beta-hemolytic group A streptococci D. Haemophilus Blood agar is usually made with defibrinated sheep blood, ______________ and nutrient agar. A. Sodium chloride B. Pig blood C. Protein D. Potassiu ...
Recommended minimal standards for description of new
... Staphylococcus and the description of newly recognized species in this genus. Assignment of a strain to the genus Staphylococcus requires that it is a Grampositive coccus that forms clusters, produces catalase, has an appropriate cell wall structure (including peptidoglycan type and teichoic acid pr ...
... Staphylococcus and the description of newly recognized species in this genus. Assignment of a strain to the genus Staphylococcus requires that it is a Grampositive coccus that forms clusters, produces catalase, has an appropriate cell wall structure (including peptidoglycan type and teichoic acid pr ...
A New Type of a Multifunctional ß
... and dehydrogenation reactions. In particular, they differ in stereospecificity of substrates required and products formed. All in all, the different enzymes of both reaction steps are assigned to two different metabolic routes. The first and most widespread route consists of a 2-enoyl-CoA hydratase, ...
... and dehydrogenation reactions. In particular, they differ in stereospecificity of substrates required and products formed. All in all, the different enzymes of both reaction steps are assigned to two different metabolic routes. The first and most widespread route consists of a 2-enoyl-CoA hydratase, ...
Metabolic fuels: regulating fluxes to select mix
... from the circulation is constrained by trans-sarcolemmal transfer. Fuel selection is performed by recruiting different muscles, different fibers within the same muscles or different pathways within the same fibers. Electromyographic analyses show that shivering humans can modulate carbohydrate oxida ...
... from the circulation is constrained by trans-sarcolemmal transfer. Fuel selection is performed by recruiting different muscles, different fibers within the same muscles or different pathways within the same fibers. Electromyographic analyses show that shivering humans can modulate carbohydrate oxida ...
The Ostrich (Struthio camelus) egg
... T a b l e 3. N-terminal s e q u e n c e o f t h e ostrich l y s o z o m e d e t e r m i n e d b y a Sequencer. C o m p a r i s o n w i t h t h e partial s t r u c t u r e s o f s w a n (12) a n d goose (11) l y s o z y m e s : o n l y the r e p l a c e m e n t s were noted. M e t h o d o f i d e n t ...
... T a b l e 3. N-terminal s e q u e n c e o f t h e ostrich l y s o z o m e d e t e r m i n e d b y a Sequencer. C o m p a r i s o n w i t h t h e partial s t r u c t u r e s o f s w a n (12) a n d goose (11) l y s o z y m e s : o n l y the r e p l a c e m e n t s were noted. M e t h o d o f i d e n t ...
Tissue culture infectivity of different strains of infectious bursal
... Two types of strains of serotype I of infectious bursal disease virus (IBDV) have been described, on the basis of their ability (IBDV-TC) or inability (IBDV-BU) to infect chicken embryonic cells in culture. However, both types infect B lymphocytes in the bursa of Fabricius of young chickens. To dete ...
... Two types of strains of serotype I of infectious bursal disease virus (IBDV) have been described, on the basis of their ability (IBDV-TC) or inability (IBDV-BU) to infect chicken embryonic cells in culture. However, both types infect B lymphocytes in the bursa of Fabricius of young chickens. To dete ...
A New Type of a Multifunctional ß-Oxidation
... and dehydrogenation reactions. In particular, they differ in stereospecificity of substrates required and products formed. All in all, the different enzymes of both reaction steps are assigned to two different metabolic routes. The first and most widespread route consists of a 2-enoyl-CoA hydratase, ...
... and dehydrogenation reactions. In particular, they differ in stereospecificity of substrates required and products formed. All in all, the different enzymes of both reaction steps are assigned to two different metabolic routes. The first and most widespread route consists of a 2-enoyl-CoA hydratase, ...
Mutation of a Highly Conserved Aspartic Acid in the Adrenergic
... fluid was added and the samples counted. Determination of basal activity was performed in quadruplicate, ligand-stimulated activity in duplicate. In each experiment, the expression of the receptor constructs was assessed in a binding assay, performed as described above, using a single saturating con ...
... fluid was added and the samples counted. Determination of basal activity was performed in quadruplicate, ligand-stimulated activity in duplicate. In each experiment, the expression of the receptor constructs was assessed in a binding assay, performed as described above, using a single saturating con ...
From transporter to transceptor
... from a high level of the transporter in the membrane. The Gap1 general amino acid permease in the yeast Saccharomyces cerevisiae has been studied as a model system for substrate-regulated intracellular trafficking. Research on this protein has revealed a complex set of regulatory mechanisms, not onl ...
... from a high level of the transporter in the membrane. The Gap1 general amino acid permease in the yeast Saccharomyces cerevisiae has been studied as a model system for substrate-regulated intracellular trafficking. Research on this protein has revealed a complex set of regulatory mechanisms, not onl ...
The Antiallergic Mast Cell Stabilizers Lodoxamide and Bufrolin as
... 2012; Funke et al., 2013; Neetoo-Isseljee et al., 2013), many of these are either of modest potency and/or display markedly different potency at human and rodent orthologs of GPR35 (Jenkins et al., 2010; Funke et al., 2013; Neetoo-Isseljee et al., 2013). This has posed challenges both in efforts to ...
... 2012; Funke et al., 2013; Neetoo-Isseljee et al., 2013), many of these are either of modest potency and/or display markedly different potency at human and rodent orthologs of GPR35 (Jenkins et al., 2010; Funke et al., 2013; Neetoo-Isseljee et al., 2013). This has posed challenges both in efforts to ...
Protein digestion and amino acid absorption along
... acids. This phenomenon was recently confirmed by Murai et a/. (1984). The free amino acid fraction was highest in carp intestinal content 3 h after a meal, but 6 h after feeding (Dabrowski, 1983a) it had decreased to less than the level found in the present trial 2.5 h after feeding. However, water ...
... acids. This phenomenon was recently confirmed by Murai et a/. (1984). The free amino acid fraction was highest in carp intestinal content 3 h after a meal, but 6 h after feeding (Dabrowski, 1983a) it had decreased to less than the level found in the present trial 2.5 h after feeding. However, water ...
The Antiallergic Mast Cell Stabilizers Lodoxamide and Bufrolin as
... 2012; Funke et al., 2013; Neetoo-Isseljee et al., 2013), many of these are either of modest potency and/or display markedly different potency at human and rodent orthologs of GPR35 (Jenkins et al., 2010; Funke et al., 2013; Neetoo-Isseljee et al., 2013). This has posed challenges both in efforts to ...
... 2012; Funke et al., 2013; Neetoo-Isseljee et al., 2013), many of these are either of modest potency and/or display markedly different potency at human and rodent orthologs of GPR35 (Jenkins et al., 2010; Funke et al., 2013; Neetoo-Isseljee et al., 2013). This has posed challenges both in efforts to ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... Gly553 in determining the acyl-CoA specificity of both enzymes. Models for the enzymes containing Met (wt CrAT and COT G553M) are structurally equivalent and reveal a shallow cavity that it is only accessible to short-chain acyl-CoAs. In contrast, the model for CrAT mutant M564G is very similar to t ...
... Gly553 in determining the acyl-CoA specificity of both enzymes. Models for the enzymes containing Met (wt CrAT and COT G553M) are structurally equivalent and reveal a shallow cavity that it is only accessible to short-chain acyl-CoAs. In contrast, the model for CrAT mutant M564G is very similar to t ...
Properties and Kinetic Analysis of UDP
... UDP-glucuronic acid is used by many pathogenic bacteria in the construction of an antiphagocytic capsule that is required for virulence. The enzyme UDP-glucose dehydrogenase catalyzes the NAD1-dependent 2-fold oxidation of UDP-glucose and provides a source of the acid. In the present study the recom ...
... UDP-glucuronic acid is used by many pathogenic bacteria in the construction of an antiphagocytic capsule that is required for virulence. The enzyme UDP-glucose dehydrogenase catalyzes the NAD1-dependent 2-fold oxidation of UDP-glucose and provides a source of the acid. In the present study the recom ...
Amino acid composition of the major ampullate gland silk
... Oneof the most difficult problems in the study of structural proteins (e.g., silk, collagen, elastin, resilin, and keratin) is solubilization without degradation of the polymer (Lucas et al. 1958). N. clavipes dragline silk, like other insect and arachnid fibroins, does not dissolve in water; nor do ...
... Oneof the most difficult problems in the study of structural proteins (e.g., silk, collagen, elastin, resilin, and keratin) is solubilization without degradation of the polymer (Lucas et al. 1958). N. clavipes dragline silk, like other insect and arachnid fibroins, does not dissolve in water; nor do ...
Paenibacillus xanthinilyticus sp. nov., isolated from agricultural soil
... Fig. 2. Phylogenetic tree reconstructed from a comparative analysis of 16S rRNA gene sequences showing the relationships between strain 11N27T and related species. Bootstrap values (expressed as percentages of 1000 replications) .50 % are shown at branch points. Filled circles indicate that the corr ...
... Fig. 2. Phylogenetic tree reconstructed from a comparative analysis of 16S rRNA gene sequences showing the relationships between strain 11N27T and related species. Bootstrap values (expressed as percentages of 1000 replications) .50 % are shown at branch points. Filled circles indicate that the corr ...
Arterial Blood Gas Interpretation: The Basics
... the carotid and aortic bodies. Consequently PCO2 falls below normal, equation (1) is pushed towards the left and H+ ion concentration falls. This respiratory compensation contributes to increase the acidic pH towards normal, but not completely. The respiratory system responds to metabolic acidosis q ...
... the carotid and aortic bodies. Consequently PCO2 falls below normal, equation (1) is pushed towards the left and H+ ion concentration falls. This respiratory compensation contributes to increase the acidic pH towards normal, but not completely. The respiratory system responds to metabolic acidosis q ...
Exercises 7/8/9/10/17 Endospore Stain: Exercise 7
... Acid fast microbes are pathogenic for humans, therefore observing them is critical. The diagnostic value of acid-fast stain is that pathogens retain color even in presence of acid. For example tuberculosis would retain the red dye carbolfuchsin even after it was washed with a powerful solvent made o ...
... Acid fast microbes are pathogenic for humans, therefore observing them is critical. The diagnostic value of acid-fast stain is that pathogens retain color even in presence of acid. For example tuberculosis would retain the red dye carbolfuchsin even after it was washed with a powerful solvent made o ...
Hepoxilin
Hepoxilins (HxA3 and HxB3) are nonclassic eicosanoid hormones involved in inflammation.