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ENERGY Life depends on energy, which is stored in the chemical bonds of energy storing compounds. One of the most important energy compounds is ATP (adenosine triphosphate). I. ATP A. made of a sugar, adenine, and 3 phosphates 1. ATP releases chemical energy whenever a bond holding a phosphate is broken A A S P P P Energy released when bond is broken Adenine 2. The result is ADP – adenosine diphosphate A Adenine A S P P B. ATP is used for doing work in the cell 1. provides energy for the mechanical functions of cells 2. provides energy for active transport of molecules and ions across the cell membrane 3. used in making and breaking down large molecules C. Because cells are constantly at work, they need an endless supply of ATP 1. ATP comes from attaching a phosphate to an ADP 2. The cycle of making and breaking down ATP molecules occurs constantly in cells 10 million new ATP molecules are made EVERY SECOND in the cell II. Sources of Energy Organisms are classified into 2 groups according to how they get food: autotrophs and heterotrophs A. Autotrophs: can make their own food Ex. green plants use CO2 , water, and sunlight to make food 1. the foods made by autotrophs are mainly carbohydrates such as glucose 2. autotrophs are called producers because they produce their own food 3. producers are vital to the world: they are a source of food for all other organisms, directly or indirectly B. Heterotrophs: organisms that can’t make their own food 1. must eat, or consume, other organisms for food 2. heterotrophs are called consumers I. Photosynthesis Photosynthesis is the process by which autotrophs convert sunlight into a usable form of energy A. autotrophs that perform photosynthesis contain pigments 1. pigment: molecule that absorbs certain wavelengths of light and reflects others whatever wavelength is reflected is the color you see 2. chlorophyll: pigment that is used in photosynthesis absorbs blue, violet, and red light reflects green light, gives the green color of many plants B. In many autotrophs, the pigments (including chlorophyll) are in specialized organelles chlorophyll is located in the chloroplasts II. Photosynthesis in a Nutshell A. 6 CO2 + 6 H2O carbon dioxide water Light energy C6H12O6 + 6 O2 glucose oxygen 1. The energy stored in glucose is used later to produce ATP. B. Photosynthesis does NOT happen all at once. There are 2 distinct stages. 1. The first stage is called the light dependent reactions. a. begins with light hitting the chloroplast b. water is split into hydrogen ions, oxygen, and excited electrons oxygen diffuses out of the chloroplast NADPH and ATP are produced (NADPH is another energy storing molecule) LIGHT REACTIONS OCCUR IN THE THYLAKOID. 2. The second stage of photosynthesis is called the Calvin Cycle. (also called the “dark reactions”) a. the ATP and NADPH from the light dependent reactions are used in the Calvin cycle b. the Calvin cycle uses CO2 to produce sugar (glucose is a type of sugar) III. Details: The Light Reactions A. occur in different areas of the thylakoid called Photosystem I and Photosystem II, which are light collecting units of the chloroplast 1. Sunlight comes into Photosystem II and splits water into H+ ions (hydrogen ions), oxygen (O2), and energized electrons (e-- ). 2. The excited e— go through the electron transport chain to Photosystem I. carrier molecules (of the e— transport chain) use the electrons’ energy to actively transport H+ ions from the stroma to the thylakoid IV. Calvin Cycle – Details (a.k.a. Dark Reactions) A. The Calvin Cycle : 1. Requires the products of the light dependent reactions 2. Requires the input of CO2 3. Takes place in the stroma of the chloroplast B. Process: 1. CO2 is used to build molecules of glucose 2. ATP and NADPH are used for energy and hydrogen (from light dependent reactions) 3. 1 molecule of glucose is made for every 6 molecules of CO2 in the cycle V. Now what? A. Photosynthesis gets glucose from the energy of the sun 1. autotrophs and heterotrophs convert glucose to ATP and use the ATP for energy 2. any glucose not used by the autotrophs right away is stored as starch 3. when autotrophs are consumed by heterotrophs, the starch is broken down into glucose 4. glucose is broken down to release energy in the process of CELLULAR RESPIRATION Cellular Respiration *** All organisms rely on cellular respiration for the energy they need to carry out life functions. I. Cellular Respiration A. process of getting ATP molecules from glucose B. usually occurs in the mitochondria II. 2 types of cellular respiration: Aerobic and Anaerobic A. Aerobic respiration requires oxygen 1. produces 36 ATP molecules from each glucose molecule 2. has 3 distinct phases: a. glycolysis glucose 2 pyruvate + 2 ATP b. Krebs Cycle pyruvate Acetyl-CoA Krebs Cycle CO2 + NADH + FADH2 + ATP NADH and FADH2 are energy storing molecules. Since you get 2 pyruvate from each glucose, the Krebs cycle gets 2 more ATP. *** So far, glycolysis and the Krebs cycle have gotten 4 ATP. 3. Electron Transport: transfers energy in the electrons of NADH and FADH2 to ATP a. this is the part of aerobic cellular respiration that requires oxygen b. electron transport generates 32 ATP molecules **Aerobic respiration generates a total of 36 ATP molecules. B. The other type of cellular respiration is anaerobic respiration (also known as fermentation). 1. releases energy from food molecules in the absence of oxygen 2. 2 types of anaerobic respiration: a. alcoholic fermentation b. lactic acid fermentation 3. In both types of anaerobic fermentation, only 2 ATP molecules are made from each molecule of glucose. C. Alcoholic Fermentation – Steps 1. glycolysis (just as in aerobic respiration) glucose 2 pyruvate + 2 ATP 2. pyruvate ethanol + CO2 This process is used to raise bread, make wine, and brew beer by using yeasts which perform alcoholic fermentation. D. Lactic Acid Fermentation 1. Animal cells can’t perform alcoholic fermentation, but some animal cells can convert pyruvate to lactic acid. Example: muscle cells switch to anaerobic respiration when there isn’t enough O2 from breathing, such as during strenuous exercise. Muscle fatigue and soreness is from a build up of lactic acid. 2. Steps of Lactic Acid Fermentation a. glycolysis: just like in aerobic respiration and alcoholic fermentation b. pyruvate + NADH lactic acid + NAD+