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Chapter 15-16 Packet
1.
Outline 15a
2.
Outline 15b
3.
Outline 16a
4.
Outline 16b
5.
Ch.15 Review
6.
Ch.16 Review
7.
4 X 6 card
Ch.15-16 Review
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Know difference between in vivo and ex vivo gene therapy
Know what restriction enzymes, “sticky ends” are, their function, and how they are
useful in recombinant technology, where restriction enzymes come from
Know the functions of promoter, operator, enhancer, regulator sites on DNA
Know the different levels of control/their order in gene activity/expression in
eukaryotes
Know everything about the lac operon; iducible /repressor operon and CAP
transcription activator
Know everything about the trp operon; repressible operon, repressor
Know how gene control/regulation differs in prokaryotes and eukaryotes
Know how different types of mutations affect translation differently, point
mutations, frameshift mutations
Know exactly how sickle cell anemia is caused
Know how mutations in certain genes can lead to cancer, factors that increase
likelihood of developing cancer, examples of carcinogens
Know what “jumping genes” are
Know the steps used to clone genes in bacteria producing recombinant DNA
Know how DNA ligase is used in recombinant technology
Know what PCR is and is used for
Know what gel electrophoresis is and what it is used for
Know what barr bodies, heterochromatin and euchromatin are
Know how reverse transcriptase is useful in cloning genes, where it comes from,
what cDNA is
Know why bacteria cannot translate eukaryotic genes, and how this is overcome
Know various examples of transgenic plant/products
Know what is meant by a vector in biotechnology
Know examples of uses of biotechnology
Know terms: bioinformatics, genomics, proteomics
Know what gene therapy is, and how it has been used
Free Response
Know the steps of how to create recombinant DNA/genetic engineering/cloning
genes to produce desired gene products/genes
Example 98-2 on sample essays handout would be a good example to know