Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download WV Department of Agriculture
Transcript
Animal Health Division Brenda Keavey Aquatic Health Certifications West Virginia Department of Agriculture’s Laboratory in Charleston History • first fish bacterial cultures performed - 1990’s • WV Dept. of Education samples • Few requests from producers (approximately 2 requests annually) http://www.picsearch.com/pictures/animals/fish/fish%20to-z/trout.html West Virginia Department of Agriculture’s Laboratory in Charleston Progression of Laboratory Testing for Fish 2006 2007 Sampling training at Micro Technologies Inc. Diagnostic testing training at Micro Technologies Inc. Sampling procedures training Testing areas included bacterial, viral, molecular and parasitic West Virginia Department of Agriculture’s Laboratory in Charleston Progression of Laboratory Testing for Fish 2008 Obtained Viral Hemorrhagic Septicemia Virus (VHSV) permit for positive cultures. Prepared for DNR testing – USDA grant for surveillance of VHSV. Performed viral cell culture transfers and testing samples for approximately 1 year. 2009 Suspended cell culture testing due to lack of samples and direction of lab’s role in aquaculture. 2010 Cell culture testing reinstated at the end of the year due to the formation of the Aquaculture Board. West Virginia Department of Agriculture’s Laboratory in Charleston Current Laboratory Testing Goals for 2011- Bacterial Cultures Bacterial cultures for a number of organisms Aeromonas species Streptococcus species Carnobacterium piscicola Vibrio species Edwardsiella species Yersinia ruckeri Flavobacterium species West Virginia Department of Agriculture’s Laboratory in Charleston Current Laboratory Testing Goals for 2011 - Training Rob Nottingham, Laboratory Supervisor Animal Health Division Microbiologist Regulatory and Environmental Affairs Division West Virginia Department of Agriculture’s Laboratory in Charleston Current Laboratory Testing Goals for 2011- Certifications Become certified by USDA to perform cell culture for Viral Hemorrhagic Septicemia Virus (VHSV) Infectious Hematopoeitic Necrosis Virus (IHNV) Cytopathic effect in FHM cell line 4 days after inoculation Courtesy of AFS Bluebook West Virginia Department of Agriculture’s Laboratory in Charleston Laboratory Testing Goals for 2011 – 2012 Bacterial Diseases of Fish Renibacterium salmoninarum (Bacterial Kidney Disease) Cultural Techniques Fluorescent Antibody Test (FAT) Polymerase Chain Reaction (PCR) Enzymed-Linked Immunosorbent Assay (ELISA) Courtesy of 2010 edition of the American Fisheries Society, Fish Health Section Blue Book West Virginia Department of Agriculture’s Laboratory in Charleston Laboratory Testing Goals for 2011 – 2012 Viral Diseases of Fish Infectious Pancreatic Necrosis Virus (IPN) Cell Culture Fluorescent Antibody Test (FAT) Polymerase Chain Reaction (PCR) Birnavirus: The Queen's University of Belfast, kindly provided by Prof Stewart McNulty West Virginia Department of Agriculture’s Laboratory in Charleston Laboratory Testing Goals for 2011 – 2012 Parasitic Diseases of Fish Tetracapsuloides bryosalmonae Proliferative Kidney Disease (PKD) Presumptive diagnosis - staining confirmation histopathology Courtesy of 2010 edition of the American Fisheries Society, Fish Health Section Blue Book West Virginia Department of Agriculture’s Laboratory in Charleston Laboratory Testing Goals for 2011 – 2012 Parasitic Diseases of Fish Ceratomyxa shasta Myxobolus cerebralis (Whirling Disease) ANY QUESTIONS?
