Download Biosafety in Microbiological and Biomedical Laboratories, pp

Biosafety in Microbiological and Biomedical Laboratories. 4th ed. JY Richmond,
RW McKinney, eds. 1999. Health and Human Services Dept, Public Health Service,
Centers for Disease Control and Prevention, National Institute of Health. ISBN:
0788185136.
Text can be downloaded from the following website:
http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm
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Section VIIG – Arboviruses and Related Zoonotic Viruses (pp. 183-199)
QUESTIONS
1) Arboviruses are a large group of viruses that are spread by certain invertebrate animals
(arthropods), most commonly blood-sucking insects (In USA. spread mainly by
mosquitoes) T/F
2) Most people infected with arboviruses have few or no symptoms, but arboviruses can
cause serious and potentially fatal inflammation of the brain (encephalitis) as well as
other complications. T/F
3) Prevention depends mainly on public health action to control mosquitoes and on
individual action to avoid mosquito bites. T/F
4) To export bluetongue virus and eastern encephalomyelitis virus Department of
Commerce license are not required. T/F
5) Importation and domestic transfer for the above agents can be obtained from ----------.
6)Infection of newly hatched chicken with eastern and western encephalomyelitis virus is
hazardous and should be undertaken only by immunized personnel under BSL3
conditions. T/F
7) Investigational vaccines against eastern and western equine encephalomyelitis viruses
are available through __________ and _____________________.
8) The use of these above two vaccine is recommended for personnel who works directly
and regularly in the laboratory. T/F
9) Dengu virus in the laboratory requires strict adherence to ___________.
10) The U.S. Departments of Health and Human Services (HHS) and Agriculture
(USDA) published final rules, which implement the provisions of the USA PATRIOT
Act and Public Health Security and Bioterrorism Preparedness and Response Act of 2002
setting forth the requirements for possession, use, and transfer of select agents and toxins.
T/F
11) The final rules (42 C.F.R. Part 73, 7 C.F.R. Part 331, and 9 C.F.R. Part 121) were
published in the Federal Register on March 18, 2005. T/F
12) A single form number will be used (Effective March 18th 2005) for each of the
identical forms used by HHS and USDA (e.g. "Application for Laboratory Registration
for Possession, Use, and Transfer of Select Agents and Toxins" which was previously
reference as CDC Form 0.1319 or APHIS Form 2040 will now be reference as
APHIS/CDC Form 1). T/F
13) Arenavirus: One of a family of viruses called Arenaviridae whose members are
generally associated with diseases not transmitted by rodents to humans. T/F
14) The first arenavirus, lymphocytic choriomeningitis virus (LCMV), was isolated in
1933, of significant important to laboratory animal medicine. T/F
15) Arboviruses assigned to BSL2 of significance to Veterinary medicine includes
__________________ and __________________.
16) Vaccine strains of ABSL3/4 which may be handled at BSL2
a)
b)
c)
d)
e)
Junin
Yellow fever
Rift Valley Fever
Venezulean equine encephalomyelitis
All of the above
17) Arboviruses and certain other viruses assigned to BSL3.
a)
b)
c)
d)
e)
Venezulean equine encephalomyelitis
Yellow fever
West Nile
Vessicular stomatitis
All of the above
18) A licensed attenuated live virus is available for immunization against Yellow fever
and is recommended for all personnel who works with yellow fever infected animals. T/F
19) For Venezulean equine encephalomyelitis , investigational (IND) vaccine TC-83
provides excellent protection against many epizootic strains. T/F
20) Decisions regarding unidentified virus isolated from human beings should be handled
at BSL-3 or preferably at BSl-4, unless there is confidence that the agent is not infectious
via the aerosol route. T/F
21) Arbovirus, Arenavirusa and Filovirus assigned to BSL-4
a) Congo-crimean hemorrhagic fever
b) Ebola
c) Lassa
d) Marburg
e) Kyasanur Forest disease
f) All of the above
Section VIIG – Arboviruses and Related Zoonotic Viruses (pp. 183-199)
ANSWERS
1) T
2) T
3) T
4) F
5) USDA/APHIS/VS
6) T
7) CDC and U.S. ARMY Medical Research Inst. For Infectious Diseases
8) T
9) BSL-2 practices.
10) T
11) T
12) T
13) F
14) T
15) Eastern and western encephalomyelitis virus
16) e
17) e
18) T
19) T
20) T
21) f