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國立彰化師範大學 95 學年度碩士班招生考試試題
系所：生物技術研究所
科目：生物化學
☆☆請在答案紙上作答☆☆
共 4 頁，第 1 頁
一、選擇題 各 2 分 共 30 分
1. The conversion of palmitoyl-CoA to stearyl-CoA requires
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(a). Δ desaturase
(b). fatty acid synthase
(c). malonyl-ACP (acyl carrier protein)
+
(d). NAD
(e). NADPH
2. The following metabolic pathways occur in the cytoplasm except:
(a). the pentose phosphate pathway.
(b). TCA cycle
(c). cholesterol biosynthesis
(d). fatty acid biosythesis
(e). nucleotide biosynthesis.
3. Which of the following is NOT a step in the phosphoinositide cascade?
(a). hormone binds a cell surface receptor
(b). occupied receptor causes GTP-GDP exchange
(c).activated PLC cleaves PIP2 to DAG and IP3
(d). DAG binds a receptor in the endoplasmic reticulum, which releases Ca2+ and activates
protein kinase C
(e). phosphorylation of cellular proteins by protein kinase C produces cellular response
4.
Acetyl-CoA is a key molecule in metabolism, because it can be converted into all of the
following except:
(a). cholesterol
(b). cysteine
(c). ketone bodies
(d). fatty acids
(e). TCA cycle intermediates (e.g., citrate)
(f). all of the above
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國立彰化師範大學 95 學年度碩士班招生考試試題
系所：生物技術研究所
科目：生物化學
☆☆請在答案紙上作答☆☆
共 4 頁，第 2 頁
5. The synthesis of a fatty acid by fatty acid synthase will
(a). produce a 16 carbon polyunsaturated fatty acid.
(b). require NADH as a source of reducing agents.
(c). use acetyl-CoA as a source of 2 C units.
(d). be driven by release of CO2
(e). make a C16 fatty acid in which the last 2 carbons added are carbon 15 and 16.
6. Enzymes
(a). Increase the velocity of chemical reactions.
(b). Change the free energies of the reactants and products.
(c). Increase the free energy of activation.
(d). Are consumed during the reactions they catalyze.
(e). Change the equilibrium of the reactions they catalyze.
7.
In a plot of l/V against 1/[S] for an enzyme-catalyzed reaction, the presence of a competitive
inhibitor will alter the:
(a). Vmax.
(b). intercept on the l/V axis.
(c). intercept on the l/[S] axis.
(d). curvature of the plot.
(e). pKa of the plot.
8. In the Michaelis-Menten plot below, the uninhibited reaction is described by curve 1.
The effect of a competitive inhibitor is represented by curve:
(a). 1.
(b). 2.
(c). 3.
(d). 4.
(e). None of the curves
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國立彰化師範大學 95 學年度碩士班招生考試試題
系所：生物技術研究所
科目：生物化學
☆☆請在答案紙上作答☆☆
共 4 頁，第 3 頁
9. All of the following enzymes are required in the catabolism of purine nucleotides EXCEPT:
(a). xanthine oxidase
(b). purine nucleoside phosphorylase
(c). 5'-nucleotidase
(d). adenosine deaminase
(e). ribonucleotide reductase
10. The direct sources of nitrogen that are used to make urea via the Urea Cycle are:
(a). citrulline and ornithine
(b). arginine and aspartate
(c). arginine and citrulline
(d). ammonia and arginine
(e). aspartate and ammonia
11. Which product in glycolysis also involve in serine synthesis?
(a) G6P. (b) F6P. (c) 3PG. (d) G3P. (e) Pyruvate.
12. How many ATP are generated by converting 1 mole of glucose to lactate?
(a) 1 mole. (b) 2 mole. (c) 4mole. (d) 30 mole. (e) 36 mole. (f) 38 mole.
13. Which of following do not involved in pyruvate oxidation and the citric acid cycle?
(a) Lipoic acid. (b) FAD. (c) NAD+. (d) Coenzyme A (e) Ascorbic acid
(f) Thiamine pyrophosphate.
14. Which of following belong to essential amino acid?
(a) Asparagin. (b) Tyrosine. (c) Glutamine. (d) Isoleucine. (e) Serine.
15. Avidin is a protein that binds extremely tightly to biotin. Therefore, it is a potent inhibitor of
biotin-requiring enzyme reactions. Consider glucose biosynthesis from each of the following
substrate and predict which of these pathways would be inhibited by avidin?
(a) Lactate. (b) Oxaloacetate. (c) Fructose-6-phosphate. (d)Phosphoenolpyruvate.
(e) Fructose-1,6-biphosphate.
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國立彰化師範大學 95 學年度碩士班招生考試試題
系所：生物技術研究所
科目：生物化學
☆☆請在答案紙上作答☆☆
共 4 頁，第 4 頁
二、問答題 共 70 分
1.
Fill in the blank to the left of each description of a metabolic process with the appropriate letter
from those listed in the box. There is only one answer for each blank, 1 point each (3%)
A. Transamination
B. Urea Cycle
C. Glycogenolysis
E. Decarboxylation
F. Compartmentation
G. Ketogenesis
I. Lipogenesis
J. Gluconeogenesis
K. Glycogenesis
D. Deamination
H. β-Oxidation
L. Glycolysis
______
The process converting ammonia originating from amino acids into nitrogenous waste
excreted by the kidneys.
______
The process of converting fatty acids (even-chain) into acetyl-CoA units in the
mitochondria.
______
The removal of an amine group from an amino acid, which produces free ammonia.
2. Use the skeletal muscle contraction system as a model for cellular communication. Describe 6
common themes of cellular communication. (12%)
3. About “Pentose phosphate pathway”. What functions is it? What’s the oxidative part of it? What
enzymes are involved in the nonoxidative part? (10%)
4. Glucagon activates liver phosphorylase via cAMP as its secondary messenger. Muscle does the
same with epinephrine stimulation. Why the cAMP has quite different effects on liver and muscle
glycolysis. Explain detail. (5%)
5. How is amino nitrogen in peripheral tissue transport to the liver for conversion to urea? (5%)
6. Please draw the Amino Acids: Q, C, E and R.(8%)
7. Please draw the Watson-Crick model of DNA.(8%)
8. Please describe the secondary structures of polypeptides.(8%)
9. Please describe the differences of the Gram positive and Gram negative cell walls.(6%)
10. Please describe the saturated fatty acid and the unsaturated fatty acid.(5%)
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