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SBI 4U Mrs. Holterman Name: __________________ Date: __________________ Glycolysis Questions Using the diagram provided and page 65-66, answer the following questions. 1. Where does glycolysis occur? Cytoplasm in cells of almost all species 2. a) How many ATP are used? 2 b) How many ATP are made? 4 3. What happens to the Pi group lost from ATP? It becomes part of the glucose molecule (or its derivative – F6P). 4. Where does the Pi group come from when ATP is made? The glucose derivative (BPG and PEP) 5. How many enzymes are used in glycolysis? 10 6. Identify coupled reactions in Glycolysis. 1, 3, 6, 7, 10 7. Explain substrate-level phosphorylation in your own words. When a substrate gains a phosphate from another molecule. 8. What type of enzyme is used in the step: DHAP G3P? Isomerase F1,6BP G3P? Lyase PEP Pyruvate? Kinase 9. If you burn glucose, you get 2870 kJ of energy. ATP stores 30.5 kJ. How efficient is glycolysis alone? (Keep in mind your answers to questions 1 and 2) % efficiency = (energy output)/(energy input) x 100% % efficiency = 61kJ/2870kJ x 100% = 2% 10. Identify all redox reactions in glycolysis. a. What is reduced in each of these reactions? NAD+ b. What is oxidized? Home/SBI 4U/Cell Metabolism/Glycolysis SBI 4U Mrs. Holterman Name: __________________ Date: __________________ G3P 11. Highlight where the atoms come from and/or go to in step 1, 3, 6, 7, 9, & 10. 12. Write the overall chemical equation for glycolysis. Glucose + 2ATP + 4 ADP + 2Pi + 2NAD+ → 2 Pyruvate + 2 ADP + 4 ATP + 2 NADH + 2H2O Home/SBI 4U/Cell Metabolism/Glycolysis