Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
THIRD SEMESTER – NOVEMBER 2007
AE 19
BT 3812 - PLANT BIOTECHNOLOGY
Date : 24/10/2007
Time : 9:00 - 12:00
Dept. No.
Max. : 100 Marks
PART - A
ANSWER ALL THE QUESTIONS.
I. Choose the correct answer:
(5 x 1 = 5)
1. What is the contribution of Karry Mullis’s to Biology?
a) Polymerase Chain Reaction (b) Sequencing (c) discovery of double helix
(d) discovery of transposons
2. First Biotechnology company in India is
(a) Biocon
(b) Shantha Biotech (c) Bharath Biotech
3. T DNA means
(a) Transfer DNA
(c) Transfected DNA
(d) Avesthagen
(b) Transformed DNA
(d) Transposed DNA
4. Fruit ripening hormone is
(a) Ethylene
b) Kinetin
c)Auxin
d) Gibberellic acid
5. MS medium is used for
a) Plant tissue culture b) Bacterial culture c) Yeast culture d) Animal cell culture
II. State True or False, if false give reason
(5 x 1 = 5)
6. Gene silencing can be used for turning off a gene
7. Meristem culture is used to eliminate virus in tissue culture
8. Barbara McClintok discovered jumping genes
9. Particle gun bombardment technique cannot be used for gene transfer in plants
10. Haploid set of chromosome (n) of an organism is termed as genome
III. Complete the following:
(5 x 1=5)
11. Hormones used to induce roots in vitro is______________________
12. USDA stands for _________
13. Jacob and Monad discovered ___________________
14. T - DNA is found in _______________________
15. Kanamycin can be used as a ___________________ marker
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IV. Define the following in one or two sentences within 50 words only (5 x 1=5)
16.
17.
18.
19.
20.
Which is the best method for transformation of dicots?
What is a constitutive promoter?
What is a reporter gene?
What is somatic embryogenesis?
What is the germicidal range of UV rays used in plant tissue culture?
PART B
V. Answer any five questions within 350 words only
(5 x 8= 40)
21 Explain Trp operon
22. Give an account on Human Genome Project
23. Explain Rubisco synthesis
24. Give an account on Cytoplasmic Male Sterility (CMS)
25. What are the differences between positive and negative regulation of genes in
bacteria?
26. Distinguish between a shuttle vector and expression vector and mention their uses
27. Enumerate the roles of macro and micro nutrients used in plant tissue culture
28. Explain the methods for haploid production
PART C
VI. Answer the following in detail, not more than 1500 words each (2x 20= 40)
29. (a) Describe the Lac operon
OR
(b) Describe the protocol for somatic hybridization. How are cybrids detected?
30 (a) Describe the Agrobacterium mediated gene transfer method.
OR
(b) Explain chloroplast and mitochondrial genomes (DNA)
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