Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – ZOOLOGY
FOURTH SEMESTER – April 2009
UF 37
ZO 4811 - BIOTECHNOLOGY
Date & Time: 23/04/2009 / 9:00 - 12:00 Dept. No.
Max. : 100 Marks
PART - A
Answer the following in a few sentences
(10 x 2 = 20
1. Distinguish probe from primer
2. What is palindrome sequences ?
3. State the significance of micro satellite
4. Name any two plants toxic to insects
5. Reveal the significance of Ti plasmid
6. What do you know about micro manipulation technique?
7. Comment on ipt, gox, rip and sac B genes
8. Outline the classification of biofertilizers
9. Define tripsinization and mention its significance
10. Expand :RFLP and PECAM
PART — B
Briefly answer any FOUR of the following
(4 x 10 = 40)
11. Illustrae the various steps involved in southern blotting technique with a note on its
applications
12. Tabulate any ten restriction enzymes with respect to recognition sequence and splicing
13. How do you treat petroleum sludge and oil spills biotechnologically ?
14. Design a bioleaching plant and add a note on its processing and applications.
15. Tabulate the differences between monoclonal and polyclonal antibodies
16. Propose a protocol for human skin (organ) culture with note on its applications
PART—C
Write detail answer to any TWO of the following
(2 x 20 = 40)
17. Describe any two methods of sequencing DNA
18. How do you produce transgenic animals? Explain any two methods with due illustrations
19. Examine the strategies of gene therapy in terms of malignant, cardiovascular diseases and
nervous disorders.
20. How do you produce bio proteins using single cells?
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