Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIOTECHNOLOGY
FIRST SEMESTER – NOVEMBER 2014
BT 1826 - BIOCHEMISTRY & BIOPHYSICS
Date : 03/11/2014
Time : 01:00-04:00
Dept. No.
PART –A
Answer all the questions:
I. Choose the correct answer:
1.
d. ΔG= ΔH
c. Histidine
d. Arginine
b. Serine
b. Fumarase
c. Succinate dehydrogenase
b. vertical angle
d. near vertical angle
Which of the following separates substances based on the boiling point?
a. Ion exchange chromatography
c. Mass spectrometer
b. Polyacrylamide gel electrophoresis
d. Gas chromatography
II. State whether the following are true or false; if false give reason:
6.
7.
8.
9.
10.
(5x1= 5)
The energy of the universe keeps changing.
The peptide bond has partial double bond character.
Serine is synthesized from 3-phosphoglycerate.
Metal chelate chromatography is a type of affinity chromatography.
MALDI is a detector used in mass spectrometer.
III. Complete the following:
11.
12.
13.
14.
15.
d. Citrate synthase
Choose the ideal rotor for pelleting during differential separation of biological particles?
a. fixed angle
c. swinging bucket
5.
c. ΔG = +ve
Identify the membrane bound enzyme in the following
a. Hexokinase
4.
b. ΔG= -ve
Pick out the neutral amino acid from the following:
a. Aspartate
3.
(20 Marks)
(5x1= 5)
Which of the following is true for an exergonic reaction?
a. ΔG= 0
2.
Max. : 100 Marks
The number of phosphoanhydride bonds in ATP is ________
Chitin is a polymer of __________________.
________________ is the precursor for de novo purine synthesis.
____________ is used as light source in analytical centrifuges.
_______________dye is used for staining SDS PAGE gels.
(5x1= 5)
IV. Answer the following, each within 50 words only:
16.
17.
18.
19.
20.
(5x1= 5)
Define pH.
What are enantiomers?
Mention the pathways involved in the synthesis of nuclei acid.
Which tool is used in the functional study of brain?
What is the principle behind nuclear magnetic spectroscopy?
PART-B
Answer the following, each within 500 words; Draw diagrams wherever necessary:
(5x8=40 marks)
21. a. Explain the action of phosphate buffer and bicarbonate buffer in maintaining the blood pH.
OR
b. Mention any eight properties of water.
22. a. Comment on the various membrane lipids.
OR
b. Classify amino acids based on the R groups they possess.
23. a. Write an overview on the complete metabolism of glucose.
OR
b. Write an account on phenylketonuria and Gaucher’s disease.
24. a. Illustrate the principle and instrumentation of UV spectroscopy.
OR
b. Employ a non-invasive technique to image fetal movements. .
25. a. Explain the torsion angles using Ramachandran plot.
OR
b. Briefly explain Bragg’s law and biological crystal preparation methods for X-ray diffraction.
PART-C
Answer any two of the following, each within 1500 words; Draw diagrams wherever necessary:
(2x20= 40 marks)
26. Elaborate on pH scale and pH meter. Add a note on Henderson Hasselbalch equation.
27. Write in detail about the steps involved in synthesis and degradation of fatty acids.
28. Analyze biomolecular separation using analytical centrifugation and preparative centrifugation.
29. Describe separation of proteins by electrophoresis based on their size and charge.
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