* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download PowerPoint-Präsentation
Survey
Document related concepts
Cell membrane wikipedia , lookup
Biochemical switches in the cell cycle wikipedia , lookup
Endomembrane system wikipedia , lookup
Cellular differentiation wikipedia , lookup
Cell culture wikipedia , lookup
Programmed cell death wikipedia , lookup
Extracellular matrix wikipedia , lookup
Organ-on-a-chip wikipedia , lookup
Cell growth wikipedia , lookup
Transcript
PhD Position for the … Biochemical and Molecular Analysis of the Cell Wall Glyco(prote)ome of Lactobacillus buchneri Universität für Bodenkultur Wien Dept. of NanoBiotechnology NanoGlycobiology Group Zentrum für Nanobiotechnologie NanoGlykobiologie-Gruppe Time schedule: Start: February-April 2012; Duration: 3 years Short summary of project Lactobacillus buchneri strain CD034 possesses a complex cell wall architecture. The main focus of the work lies particularly on the characterization of the glycosylated cell wall constituents of this organism, such as the two-dimensionally crystallizing S-layer glycoprotein, a putative secondary cell wall polymer (SCWP) involved in S-layer anchoring, and the identification of a predicted exopolysaccharide (EPS) which is known from other lactic acid bacteria. In this context it should be mentioned that between S-layer carrying and S-layer deficient bacteria strain-specific differences may exist regarding architecture, composition and function of specific cell wall constituents. The presence of an S-layer glycoprotein on this GRAS organism, which makes it an interesting candidate for possible future glycan-related medical and/or biotechnological applications. Research goals Bioinformatic analysis of the genome of L. buchneri CD034 for loci Schematic presentation of the cell wall of harboring genes involved in the biosynthesis of glycosylated cell Lactobacillus strains with focus on glycowall components of L. buchneri CD034 (in collaboration with Prof. sylated components R. Grabherr, IAM, BOKU). Expression and purification of selected cell wall components from L. buchneri CD034. Structural and functional characterization of glycosylated cell wall components of L. buchneri CD034 (in collaboration with Prof. P. Kosma and Prof. F. Altmann, Chemie, BOKU). Requirements The candidate must hold a MSc degree and should have a sound background in molecular biology, microbiology, biochemistry. Practical experience in the field of glycobiology is of advantage but not mandatory. The highly motivated candidate should be an excellent team player with the ability to work independently in an interdisciplinary environment. We offer Motivating scientific environment at the Vienna Institute of Biotechnology, cutting edge research in nanoglycobiology, weekly seminars, participation in workshops and international conferences. Stays in partner laboratories abroad are possible. Funding opportunity according to the guidelines of FWF (http://www.fwf.ac.at/de/projects/einzelprojekte.html). Possibility of embedding the PhD thesis into the International PhD Programme BioToP (http://biotop.boku.ac.at/). Applicants Please e-mail your application with motivation, full CV and references along with contact addresses to: Ao.Univ. Prof. Dr. Paul MESSNER Department für NanoBiotechnologie, Universität für Bodenkultur Wien, Muthgasse 11, 1190 Wien e-mail: [email protected]; Phone: 47654-2202 University of Natural Resources and Applied Life Sciences, Vienna Centre for Nanobiotechnology