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Chapter 9: Biotechnology and DNA Technology Biotechnology Tools of Biotechnology Recombinant DNA Applications of rDNA Products of Genetic Engineering $100 $100 $100 $100 $100 $200 $200 $200 $200 $200 $300 $300 $300 $300 $300 $400 $400 $400 $400 $400 $500 $500 $500 $500 $500 FINAL ROUND © 2013 Pearson Education, Inc. Topic 1: Biotechnology $100 Question Today, chemicals that organisms do NOT naturally make are made by microorganisms and a. b. c. d. plants. fungi. protozoa. helminths. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 1: Biotechnology $100 Answer Today, chemicals that organisms do NOT naturally make are made by microorganisms and a. b. c. d. plants. fungi. protozoa. helminths. BACK TO GAME © 2013 Pearson Education, Inc. Topic 1: Biotechnology $200 Question When E. coli has the ability to produce the hormone insulin, what is the reason? a. b. c. d. It picked up the insulin gene from another cell. It is an ancient gene that now has no function. The insulin gene was inserted into it. It needs to regulate its cell glucose level. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 1: Biotechnology $200 Answer When E. coli has the ability to produce the hormone insulin, what is the reason? a. b. c. d. It picked up the insulin gene from another cell. It is an ancient gene that now has no function. The insulin gene was inserted into it. It needs to regulate its cell glucose level. BACK TO GAME © 2013 Pearson Education, Inc. Topic 1: Biotechnology $300 Question Natural selection is the process a. of inserting genes into cells by rDNA technology. b. whereby organisms with traits that make them better adapted to their environment tend to survive and reproduce more than organisms lacking these traits. c. by which scientists select desirable breeds of animals or strains of plants to cultivate. d. None of the answers is correct. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 1: Biotechnology $300 Answer Natural selection is the process a. of inserting genes into cells by rDNA technology. b. whereby organisms with traits that make them better adapted to their environment tend to survive and reproduce more than organisms lacking these traits. c. by which scientists select desirable breeds of animals or strains of plants to cultivate. d. None of the answers is correct. BACK TO GAME © 2013 Pearson Education, Inc. Topic 1: Biotechnology $400 Question In the field of genetic engineering, cDNA is important because it a. b. c. d. lacks exons. lacks introns. is really RNA. is really reverse transcriptase. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 1: Biotechnology $400 Answer In the field of genetic engineering, cDNA is important because it a. b. c. d. lacks exons. lacks introns. is really RNA. is really reverse transcriptase. BACK TO GAME © 2013 Pearson Education, Inc. Topic 1: Biotechnology $500 Question The procedure during which cells can take up DNA from the surrounding environment is called a. b. c. d. electroporation. protoplast fusion. transformation. microinjection. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 1: Biotechnology $500 Answer The procedure during which cells can take up DNA from the surrounding environment is called a. b. c. d. electroporation. protoplast fusion. transformation. microinjection. BACK TO GAME © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $100 Question Which technique amplifies small samples of DNA to quantities that are large enough for analysis? a. b. c. d. hybridization PCR RFLP DNA fingerprinting ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $100 Answer Which technique amplifies small samples of DNA to quantities that are large enough for analysis? a. b. c. d. hybridization PCR RFLP DNA fingerprinting BACK TO GAME © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $200 Question Which of the following techniques is used to identify bacteria carrying a specific gene? a. b. c. d. cloning Western blot transformation DNA probe ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $200 Answer Which of the following techniques is used to identify bacteria carrying a specific gene? a. b. c. d. cloning Western blot transformation DNA probe BACK TO GAME © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $300 Question If a restriction enzyme made a staggered cut in DNA, the ends would be termed _____ ends. a. b. c. d. flat sticky adhesive blunt ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $300 Answer If a restriction enzyme made a staggered cut in DNA, the ends would be termed _____ ends. a. b. c. d. flat sticky adhesive blunt BACK TO GAME © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $400 Question A population of genetically identical cells carrying a desired vector is called a. b. c. d. a clone. a species. a library. PCR. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $400 Answer A population of genetically identical cells carrying a desired vector is called a. b. c. d. a clone. a species. a library. PCR. BACK TO GAME © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $500 Question Which of the following methods is especially valuable in the genetic manipulation of plant and algal cells? a. b. c. d. protoplast fusion viral transduction transformation cloning ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 2: Tools of Biotechnology $500 Answer Which of the following methods is especially valuable in the genetic manipulation of plant and algal cells? a. b. c. d. protoplast fusion viral transduction transformation cloning BACK TO GAME © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $100 Question Which of the following is NOT a method for inserting foreign DNA into cells? a. b. c. d. transformation microinjection restriction digestion electroporation ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $100 Answer Which of the following is NOT a method for inserting foreign DNA into cells? a. b. c. d. transformation microinjection restriction digestion electroporation BACK TO GAME © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $200 Question Which of the following is a eukaryote used in genetic engineering? a. b. c. d. Agrobacterium tumefaciens Pseudomonas aeruginosa Saccharomyces cerevisiae Thermus aquaticus ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $200 Answer Which of the following is a eukaryote used in genetic engineering? a. b. c. d. Agrobacterium tumefaciens Pseudomonas aeruginosa Saccharomyces cerevisiae Thermus aquaticus BACK TO GAME © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $300 Question What are the two main sources of DNA that scientists use to obtain the genes in which they are interested? a. b. c. d. genomic libraries and PCR synthetic DNA and plasmids synthetic DNA and genomic libraries PCR and plasmids ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $300 Answer What are the two main sources of DNA that scientists use to obtain the genes in which they are interested? a. b. c. d. genomic libraries and PCR synthetic DNA and plasmids synthetic DNA and genomic libraries PCR and plasmids BACK TO GAME © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $400 Question Restriction enzymes are a. b. c. d. animal enzymes that splice RNA. bacterial enzymes that destroy phage DNA. animal enzymes that destroy RNA. viral enzymes that destroy host DNA. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $400 Answer Restriction enzymes are a. b. c. d. animal enzymes that splice RNA. bacterial enzymes that destroy phage DNA. animal enzymes that destroy RNA. viral enzymes that destroy host DNA. BACK TO GAME © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $500 Question Which enzyme is used to synthesize cDNA? a. b. c. d. RNA polymerase complementary polymerase reverse transcriptase DNA polymerase ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 3: Recombinant DNA $500 Answer Which enzyme is used to synthesize cDNA? a. b. c. d. RNA polymerase complementary polymerase reverse transcriptase DNA polymerase BACK TO GAME © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $100 Question In plants, the Ti plasmid can cause a. b. c. d. crown gall disease. tobacco mosaic disease. Pseudomonas. soft rot disease. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $100 Answer In plants, the Ti plasmid can cause a. b. c. d. crown gall disease. tobacco mosaic disease. Pseudomonas. soft rot disease. BACK TO GAME © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $200 Question Molecule-sized robots used to detect contamination in food are an example of a. b. c. d. forensic microbiology. nanotechnology. biotechnology. bioinformatics. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $200 Answer Molecule-sized robots used to detect contamination in food are an example of a. b. c. d. forensic microbiology. nanotechnology. biotechnology. bioinformatics. BACK TO GAME © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $300 Question What can be used to screen a sample for multiple pathogens simultaneously? a. b. c. d. DNA chips Southern blotting shotgun sequencing colony hybridization ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $300 Answer What can be used to screen a sample for multiple pathogens simultaneously? a. b. c. d. DNA chips Southern blotting shotgun sequencing colony hybridization BACK TO GAME © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $400 Question When mammalian cells are used to make protein products in genetic engineering, there is a(n) a. b. c. d. high risk of toxin production. increase in allergic reactions. low risk of toxin production. low rate of protein production. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $400 Answer When mammalian cells are used to make protein products in genetic engineering, there is a(n) a. b. c. d. high risk of toxin production. increase in allergic reactions. low risk of toxin production. low rate of protein production. BACK TO GAME © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $500 Question Which bacterium has been genetically modified to produce a human protein, gamma interferon? a. b. c. d. Bacillus thuringiensis Staphylococcus aureus Escherichia coli Pseudomonas fluorescens ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 4: Applications of rDNA $500 Answer Which bacterium has been genetically modified to produce a human protein, gamma interferon? a. b. c. d. Bacillus thuringiensis Staphylococcus aureus Escherichia coli Pseudomonas fluorescens BACK TO GAME © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $100 Question The Ti plasmid naturally occurs in a. b. c. d. Thermus aquaticus. Saccharomyces cerevisiae. Bacillus thuringiensis. Agrobacterium tumefaciens. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $100 Answer The Ti plasmid naturally occurs in a. b. c. d. Thermus aquaticus. Saccharomyces cerevisiae. Bacillus thuringiensis. Agrobacterium tumefaciens. BACK TO GAME © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $200 Question The method of using RFLPs to identify bacterial or viral pathogens is called a. b. c. d. proteomics. DNA fingerprinting. genetic screening. DNA sequencing. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $200 Answer The method of using RFLPs to identify bacterial or viral pathogens is called a. b. c. d. proteomics. DNA fingerprinting. genetic screening. DNA sequencing. BACK TO GAME © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $300 Question One of the earliest commercial products of rDNA technology was a. b. c. d. rennin. cellulase. malaria vaccine. human growth hormone. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $300 Answer One of the earliest commercial products of rDNA technology was a. b. c. d. rennin. cellulase. malaria vaccine. human growth hormone. BACK TO GAME © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $400 Question Which of the following is NOT a therapeutic application of biotechnology? a. b. c. d. creating herbicide-resistant plants producing DNA vaccines gene therapy synthesizing subunit vaccines ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $400 Answer Which of the following is NOT a therapeutic application of biotechnology? a. b. c. d. creating herbicide-resistant plants producing DNA vaccines gene therapy synthesizing subunit vaccines BACK TO GAME © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $500 Question If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is a. b. c. d. inserting the Ti into Agrobacterium. transformation of an animal cell. transformation of E. coli with Ti. splicing Ti into a plasmid. ANSWER BACK TO GAME © 2013 Pearson Education, Inc. Topic 5: Products of Genetic Engineering $500 Answer If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is a. b. c. d. inserting the Ti into Agrobacterium. transformation of an animal cell. transformation of E. coli with Ti. splicing Ti into a plasmid. BACK TO GAME © 2013 Pearson Education, Inc. FINAL ROUND Question One surprising finding in the Human Genome Project was that “junk DNA,” which does NOT code for a functional product, makes up what percentage of the human genome? a. b. c. d. 90% 95% 98% 85% ANSWER BACK TO GAME © 2013 Pearson Education, Inc. FINAL ROUND Answer One surprising finding in the Human Genome Project was that “junk DNA,” which does NOT code for a functional product, makes up what percentage of the human genome? a. b. c. d. 90% 95% 98% 85% BACK TO GAME © 2013 Pearson Education, Inc.