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Genetic Testing Jeopardy!
Waterford Union High School
Rules
• Each team sends one person per turn. They
cannot get help from their team
• First to “buzz” in gets 15 seconds to answer.
• If the first person to buzz cannot get the answer,
the second team can buzz in. Once they buzz in,
they too have 15 seconds. They also cannot get
help.
• You either gain or lose points on each turn.
• If no team buzzes within 10 seconds, the question
ends and the answer is given.
Sanger
Method
PCR
Electroph.
DNA FP’s
& RFA
Southern
Blotting
ELISA
This &
That
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Sanger Method
• This is a “fake” nucleotide that is dyed and
allows DNA to be read letter by letter by
stopping the addition of bases at random
points
• ddNTPs
Sanger Method
• This separates the DNA fragments from
longest to shortest
• Electrophoresis Gel
Sanger Method
• These are the kind of cells we’d use to
sequence DNA in most cases
• White blood cells
Sanger Method
• These are the portions of DNA that code for
actual genes and have an Open Reading Frame
• Exons
Sanger Method
• This is what is used to replicate the DNA for
the Sanger Method
• E. coli
PCR Electrophoresis
• These are the regions of DNA that have
random repeats of short sequences of bases
• STRs
PCR Electrophoresis
• Based on the information in the table below,
this suspect’s DNA was found at the scene of
the crime
• Suspect 2
PCR Electrophoresis
• This is the enzyme taken from hot springs that
is used in PCR to make copies of DNA in a
heating/cooling environment like that of PCR
• Taq polymerase
PCR Electrophoresis
• DNA is denatured in PCR using this
• Heat
PCR Electrophoresis
• This is the term for the process of separating
DNA fragments after they’ve been cut and
amplified
• Electrophoresis
DNA Fingerprints & Restriction
Fragment Analysis
• This is when variations in a person’s DNA are
examined to determine what aspects are
unique to an individual
• DNA Fingerprinting
DNA Fingerprints & Restriction
Fragment Analysis
• This where patterns created by cut DNA in a
gel are examined to determine the source of
DNA, paternity, or diagnose a disease.
• Restriction Fragment Analysis
DNA Fingerprints & Restriction
Fragment Analysis
• This kind of DNA is used to create the DNA
Fingerprint because it contains the most
variability and STRs
• Introns
DNA Fingerprints & Restriction
Fragment Analysis
• Human DNA is ___% the same from person to
person
• 99.9
DNA Fingerprints & Restriction
Fragment Analysis
• This is necessary to see the DNA after it is run
through a gel
• Dying
Southern Blotting
• This is the genetic test we’d use to diagnose a
genetic disease
• Southern Blotting
Southern Blotting
• To conduct Southern Blotting, we’d use this to
transfer DNA from the gel.
• A membrane
Southern Blotting
• To conduct Southern Blotting, we’d use this to
find a specific gene in question due to the fact
that this has a complementary sequence to
that DNA
• Probe
Southern Blotting
• A probe will usually do this if a gene is present
• Glow (bioluminescence)
Southern Blotting
• Southern Blotting does not work for this kind
of disease
• Infectious
ELISA
• This is the kind of test we’d use for infectious
disease.
• ELISA
ELISA
• ELISA can detect infectious disease by looking
for this substance
• Proteins (or antibodies)
ELISA
• These are produced to identify substances in
your body
• Antibodies
ELISA
• These are recognized by antibodies and are a
sign of a disease in the body
• Antigens
ELISA
• During ELISA, if a disease is present, the blood
sample will ______
• Show a color
This & That
• This is the kind of DNA that codes for a protein
• Exon
This & That
• This is the kind of DNA that does not code for
a protein
• Intron
This & That
• This is how we know if a stretch of DNA is an
exon or an intron
• Open Reading Frame
This & That
• This is the term for an antibody generator
• Antigen
This & That
• This is the enzyme that cuts DNA
• Restriction enzyme