Download OPEN UNIVERSITY LIBRARY PROGRAMME SEQUENCE LIST s 100/15 (1972). Tape No. 6HT/70550-

OPEN UNIVERSITY LIBRARY
Title:
PROGRAMME SEQUENCE LIST
Science course unit 15.
Producer:
J i r n StevensonContributors:
M . J . Pentz y
Stephen Hurry>
Steven Rose.
Summary:
s 100/15 (1972).
Tape No. 6HT/70550Project No. 00521/2190.
O.U. film no.
Date Recorded. 10.2.1972
1st TX. 23.4.1972.
Form
VTR.
Duration
24'03'V
591.8764
Class nos.
574.19254
cu
Part 1: Some of the techniques of the salivary amylase home
experiment are demonstrated. Part 2: The metabolism of
glucose in isolated slices of rat liver is followed.
m
Seq.
T i me.
1 • 02"
Footage.
16
Mike Pentz introduces the programme. He
points out that biological processes are
chemical•
78
Stephen Hurry, with part of the S 1OO home
experiment kit, goes through some of the procedures in the salivary amylase experiment
which students may have had trouble with. He
starts by running various solutions through
the colorimeter to arrive at a standard curve.
This is plotted on a large graph.
87
Hurry next demonstrates how a saliva sample
is collected and prepared for the main part
of the experiment.
1
5 * 20"
1|
5-55"
Sequence List.
6«5O"
100
Hurry plots the values which were obtained
from a salivary amylase experiment conducted
off camera.
7'28"
108
Mike Pentz introduces Steven Rose.
126
For part 2 of the programme, Steven Rose will
demonstrate techniques for studying reaction
sequences of glycolysis and glucose oxidation.
He begins by drawing the metabolic pathway by
which glucose is converted during glycolysis
to pyruvate which goes to C O 2 and lactate.
Rose explains why a radio-active trace would
be added to the glucose for a experiment.
143
Rose dissects out some liver tissue from a
rat and prepares it for the experiment.
8«49"
1O'O7"
Rose, with a Warburg manometer, explains how
the apparatus works. He then explains how the
COg produced in the experiment is absorbed.
Sound Cue
....1 use it.
By the way..•
S 1OO/15 ( 1972)
. . . 2. . .
PROGRAMME SEQUENCE LIST
OPEN UNIVERSITY LIBRARY
Continuation
Time
13 • OO"
15'2O"
l8«42"
23 I 27"
Sequence List
Footage
180
Rose begins the experiment to measure oxygen
uptake in the liver tissue.
2O8
Rose examines three manometers which had been
incubating for some time. He then stops the
reactions by destroying the enzymes with a dilute acid.
248
Rose explains and then demonstrates the use of
a scintillation counter for measuring COj.
puoduction from the reaction.
3O1
Rose explains and demonstrates a technique for
analysing lactic acid production enzymically.
He uses a graph which has plotted the absorbent
spectra of NAD and NADHz. obtained from a spectra
photometer. Rose looks at a curve which was
obtained for a run lasting about ^ hour.
Rose sums up.
24•O3"
3O8
Credits.
Sound Cue
...of the tissue.
Let's come back...